MSL3L1 (MSL3) (NM_001282174) Human Tagged ORF Clone

CAT#: RG237808

MSL3 (GFP-tagged) - Human male-specific lethal 3 homolog (Drosophila) (MSL3), transcript variant 6

Specifications

Product Data

• Tag: TurboGFP
• Symbol: MSL3
• Synonyms: MSL3L1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG237808 representing NM_001282174.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGAAAATGATGAAAACTAAAGAAGAACCAGAGCTTCAAACAAGAAGGGAAATGGAAGAAAGAACAATA
  ACTATAGAAATCCCTGAAGTTCTGAAGAAGCAGCTGGAGGATGATTGTTACTACATTAACAGGAGGAAA
  CGGTTAGTGAAACTTCCATGCCAGACCAACATCATAACGATTTTGGAATCCTATGTGAAGCATTTTGCT
  ATCAATGCAGCCTTTTCAGCCAATGAGAGGCCTCGTCACCATCACGTTATGCCACATGCCAACATGAAC
  GTGCATTATATCCCAGCAGAAAAGAATGTTGACCTTTGTAAGGAGATGGTGGATGGATTAAGAATAACC
  TTTGATTACACTCTCCCGTTGGTTTTACTCTATCCATATGAACAAGCTCAGTATAAAAAGGTGACTTCG
  TCTAAATTTTTTCTTCCAATTAAGGAAAGTGCCACAAGCACTAACAGGAGCCAGGAGGAACTCTCTCCC
  AGTCCGCCTTTGTTGAATCCATCCACGCCACAGTCCACAGAGAGTCAGCCGACCACCGGTGAACCAGCC
  ACCCCCAAAAGGCGCAAAGCTGAGCCAGAAGCATTGCAGTCTCTGAGGCGGTCCACGCGCCACAGTGCC
  AACTGTGACAGGCTTTCTGAGAGCAGCGCTTCACCTCAGCCCAAGCGCCGGCAGCAGGACACATCCGCC
  AGCATGCCCAAGCTCTTCCTGCACCTGGAAAAGAAGACACCTGTGCATAGCAGATCATCTTCACCTATT
  CCTCTGACTCCTAGCAAGGAAGGGAGTGCTGTGTTTGCTGGCTTTGAAGGGAGAAGAACTAATGAAATA
  AACGAGGTCCTCTCCTGGAAGCTTGTGCCTGACAATTACCCCCCAGGTGACCAGCCGCCTCCACCCTCT
  TACATTTATGGGGCACAACATTTGCTGCGATTGTTTGTGAAACTTCCAGAAATCCTTGGAAAGATGTCC
  TTTTCTGAGAAGAATCTGAAGGCTTTATTGAAGCACTTTGATCTCTTTTTGAGGTTTTTAGCAGAATAC
  CACGATGACTTCTTCCCAGAGTCGGCTTATGTCGCTGCCTGTGAGGCACATTACAGCACCAAGAACCCC
  CGGGCAATTTAT
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG237808
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MKMMKTKEEPELQTRREMEERTITIEIPEVLKKQLEDDCYYINRRKRLVKLPCQTNIITILESYVKHFA
  INAAFSANERPRHHHVMPHANMNVHYIPAEKNVDLCKEMVDGLRITFDYTLPLVLLYPYEQAQYKKVTS
  SKFFLPIKESATSTNRSQEELSPSPPLLNPSTPQSTESQPTTGEPATPKRRKAEPEALQSLRRSTRHSA
  NCDRLSESSASPQPKRRQQDTSASMPKLFLHLEKKTPVHSRSSSPIPLTPSKEGSAVFAGFEGRRTNEI
  NEVLSWKLVPDNYPPGDQPPPPSYIYGAQHLLRLFVKLPEILGKMSFSEKNLKALLKHFDLFLRFLAEY
  HDDFFPESAYVAACEAHYSTKNPRAIY
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001282174
• ORF Size: 1116 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001282174.1, NP_001269103.1
• RefSeq Size: 2276 bp
• RefSeq ORF: 1119 bp
• Locus ID: 10943
• Cytogenetics: Xp22.2
• Protein Families: Transcription Factors
• MW: 43.2 kDa
• Gene Summary: This gene encodes a nuclear protein that is similar to the product of the Drosophila male-specific lethal-3 gene. The Drosophila protein plays a critical role in a dosage-compensation pathway, which equalizes X-linked gene expression in males and females. Thus, the human protein is thought to play a similar function in chromatin remodeling and transcriptional regulation, and it has been found as part of a complex that is responsible for histone H4 lysine-16 acetylation. This gene can undergo X inactivation. Alternative splicing results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 2, 7 and 8. [provided by RefSeq, Jul 2010]