CYP3A43 (NM_001278921) Human Tagged ORF Clone

CAT#: RG237940

CYP3A43 (GFP-tagged) - Human cytochrome P450, family 3, subfamily A, polypeptide 43 (CYP3A43), transcript variant 4

Specifications

Product Data

• Tag: TurboGFP
• Symbol: CYP3A43
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG237940 representing NM_001278921.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGATCTCATTCCAAACTTTGCCATGGAAACATGGGTTCTTGTGGCTACCAGCCTGGTACTCCTCTAT
  ATTTATGGGACCCATTCACATAAACTTTTTAAGAAGCTGGGAATTCCTGGGCCAACCCCTCTGCCTTTT
  CTGGGAACTATTTTGTTCTACCTTAGGAGGTCTCTGAATAAGATTCCCAGCTGGGCGTGGTGGCTCACG
  CCTGTAATCCCAGCACTTTGGGAGGCTGAAGCAGGAGGATCACCTAAGGTCAGGAGTTCGAGACCAGCT
  TTGCCAACATGGGTCTTTGGAATTTTGACAGAGAATGTAATGAAAAATACGGAGAAATGTGGGGCACTC
  TTTCCATTTCTTACCCCAGTTTTTGAAGCCCTAAATATCGGTTTGTTTCCAAAAGATGTTACCCATTTT
  TTAAAAAATTCCATTGAAAGGATGAAAGAAAGTCGCCTCAAAGATAAACAAAAGCATCGAGTAGATTTC
  TTTCAACAGATGATCGACTCCCAGAATTCCAAAGAAACAAAGTCCCATAAAGCTCTGTCTGATCTGGAG
  CTTGTGGCCCAGTCAATTATCATCATTTTTGCTGCCTATGACACAACTAGCACCACTCTCCCCTTCATT
  ATGTATGAACTGGCCACTCACCCTGATGTCCAGCAGAAACTGCAGGAGGAGATTGACGCAGTTTTACCC
  AATAAGGCACCTGTCACCTACGATGCCCTGGTACAGATGGAGTACCTTGACATGGTGGTGAATGAAACG
  CTCAGATTATTCCCAGTTGTTAGTAGAGTTACGAGAGTCTGCAAGAAAGATATTGAAATCAATGGAGTG
  TTCATTCCCAAAGGGTTAGCAGTGATGGTTCCAATCTATGCTCTTCACCATGACCCAAAGTACTGGACA
  GAGCCTGAGAAGTTCTGCCCTGAAAGGTTCAGTAAGAAGAACAAGGACAGCATAGATCTTTACAGATAC
  ATACCTTTTGGAGCTGGACCCCGAAACTGCATTGGCATGAGGTTTGCTCTCACAAACATAAAACTTGCT
  GTCATTAGAGCACTGCAGAACTTCTCCTTCAAACCTTGTAAAGAGACTCAGATCCCACTGAAATTAGAC
  AATCTACCAATTCTTCAACCAGAAAAACCTATTGTTCTAAAAGTGCACTTAAGAGATGGGATTACAAGT
  GGACCC
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG237940
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MDLIPNFAMETWVLVATSLVLLYIYGTHSHKLFKKLGIPGPTPLPFLGTILFYLRRSLNKIPSWAWWLT
  PVIPALWEAEAGGSPKVRSSRPALPTWVFGILTENVMKNTEKCGALFPFLTPVFEALNIGLFPKDVTHF
  LKNSIERMKESRLKDKQKHRVDFFQQMIDSQNSKETKSHKALSDLELVAQSIIIIFAAYDTTSTTLPFI
  MYELATHPDVQQKLQEEIDAVLPNKAPVTYDALVQMEYLDMVVNETLRLFPVVSRVTRVCKKDIEINGV
  FIPKGLAVMVPIYALHHDPKYWTEPEKFCPERFSKKNKDSIDLYRYIPFGAGPRNCIGMRFALTNIKLA
  VIRALQNFSFKPCKETQIPLKLDNLPILQPEKPIVLKVHLRDGITSGP
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001278921
• ORF Size: 1179 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001278921.2
• RefSeq Size: 1404 bp
• RefSeq ORF: 1182 bp
• Locus ID: 64816
• Cytogenetics: 7q22.1
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Drug metabolism - cytochrome P450, Drug metabolism - other enzymes, Linoleic acid metabolism, Metabolic pathways, Metabolism of xenobiotics by cytochrome P450, Retinol metabolism
• MW: 45.3 kDa
• Gene Summary: This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The encoded protein has a low level of testosterone hydroxylase activity, and may play a role in aging mechanisms and cancer progression. This gene is part of a cluster of cytochrome P450 genes on chromosome 7q21.1. Alternate splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2013]