PDE1B (NM_001288768) Human Tagged ORF Clone

CAT#: RG237971

PDE1B (GFP-tagged) - Human phosphodiesterase 1B, calmodulin-dependent (PDE1B), transcript variant 3

Specifications

Product Data

• Tag: TurboGFP
• Symbol: PDE1B
• Synonyms: HEL-S-79p; PDE1B1; PDES1B
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG237971 representing NM_001288768.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGTTCCGGAGAACATACACCTCTGTGGGCCCCACTTACTCTACTGCGGTTCTCAACTGTCTCAAGAAC
  CTGGATCTCTGGTGCTTTGATGTCTTTTCCTTGAACCAGGCAGCAGATGACCATGCCCTGAGGACCATT
  GTTTTTGAGTTGCTGACTCGGCATAACCTCATCAGCCGCTTCAAGATTCCCACTGTGTTTTTGATGAGT
  TTCCTGGATGCCTTGGAGACAGGCTATGGGAAGTACAAGAATCCTTACCACAACCAGATCCACGCAGCC
  GATGTTACCCAGACAGTCCATTGCTTCTTGCTCCGCACAGGGATGGTGCACTGCCTGTCGGAGATTGAG
  CTCCTGGCCATCATCTTTGCTGCAGCTATCCATGATTATGAGCACACGGGCACTACCAACAGCTTCCAC
  ATCCAGACCAAGTCAGAATGTGCCATCGTGTACAATGATCGTTCAGTGCTGGAGAATCACCACATCAGC
  TCTGTTTTCCGATTGATGCAGGATGATGAGATGAACATTTTCATCAACCTCACCAAGGATGAGTTTGTA
  GAACTCCGAGCCCTGGTCATTGAGATGGTGTTGGCCACAGACATGTCCTGCCATTTCCAGCAAGTGAAG
  ACCATGAAGACAGCCTTGCAACAGCTGGAGAGGATTGACAAGCCCAAGGCCCTGTCTCTACTGCTCCAT
  GCTGCTGACATCAGCCACCCAACCAAGCAGTGGTTGGTCCACAGCCGTTGGACCAAGGCCCTCATGGAG
  GAATTCTTCCGTCAGGGTGACAAGGAGGCAGAGTTGGGCCTGCCCTTTTCTCCACTCTGTGACCGCACT
  TCCACTCTAGTGGCACAGTCTCAGATAGGGTTCATCGACTTCATTGTGGAGCCCACATTCTCTGTGCTG
  ACTGACGTGGCAGAGAAGAGTGTTCAGCCCCTGGCGGATGAGGACTCCAAGTCTAAAAACCAGCCCAGC
  TTTCAGTGGCGCCAGCCCTCTCTGGATGTGGAAGTGGGAGACCCCAACCCTGATGTGGTCAGCTTTCGT
  TCCACCTGGGTCAAGCGCATTCAGGAGAATAAGCAGAAATGGAAGGAACGGGCAGCAAGTGGCATCACC
  AACCAGATGTCCATTGACGAGCTGTCCCCCTGTGAAGAAGAGGCCCCCCCATCCCCTGCCGAAGATGAA
  CACAACCAGAATGGGAATCTGGAT
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG237971
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MFRRTYTSVGPTYSTAVLNCLKNLDLWCFDVFSLNQAADDHALRTIVFELLTRHNLISRFKIPTVFLMS
  FLDALETGYGKYKNPYHNQIHAADVTQTVHCFLLRTGMVHCLSEIELLAIIFAAAIHDYEHTGTTNSFH
  IQTKSECAIVYNDRSVLENHHISSVFRLMQDDEMNIFINLTKDEFVELRALVIEMVLATDMSCHFQQVK
  TMKTALQQLERIDKPKALSLLLHAADISHPTKQWLVHSRWTKALMEEFFRQGDKEAELGLPFSPLCDRT
  STLVAQSQIGFIDFIVEPTFSVLTDVAEKSVQPLADEDSKSKNQPSFQWRQPSLDVEVGDPNPDVVSFR
  STWVKRIQENKQKWKERAASGITNQMSIDELSPCEEEAPPSPAEDEHNQNGNLD
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001288768
• ORF Size: 1197 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001288768.1, NP_001275697.1
• RefSeq Size: 3525 bp
• RefSeq ORF: 1200 bp
• Locus ID: 5153
• Cytogenetics: 12q13.2
• Protein Families: Druggable Genome
• Protein Pathways: Calcium signaling pathway, Progesterone-mediated oocyte maturation, Purine metabolism
• MW: 46 kDa
• Gene Summary: 'The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase (PDE) family, and PDE1 subfamily. Members of the PDE1 family are calmodulin-dependent PDEs that are stimulated by a calcium-calmodulin complex. This PDE has dual-specificity for the second messengers, cAMP and cGMP, with a preference for cGMP as a substrate. cAMP and cGMP function as key regulators of many important physiological processes. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.[provided by RefSeq, Jul 2011]'