Chimaerin 2 (CHN2) (NM_001293071) Human Tagged ORF Clone

CAT#: RG238206

CHN2 (GFP-tagged) - Human chimerin 2 (CHN2), transcript variant 5

Specifications

Product Data

• Tag: TurboGFP
• Symbol: CHN2
• Synonyms: ARHGAP3; BCH; CHN2-3; RHOGAP3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG238206 representing NM_001293071.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGCTGAAGAATACCAGCCTCCTATATGGAAATCATACTGTGGAAAACAGACCAAAATATTATGGAAGA
  GAGTTTCATGGGATCATCTCTCGGGAGCAGGCGGATGAGCTTCTTGGAGGCGTGGAGGGTGCCTACATC
  CTTAGAGAAAGCCAGCGGCAACCAGGATGCTACACGCTGGCTCTCAGGTTTGGAAACCAGACCTTAAAC
  TACAGGCTCTTCCACGACGGGAAACACTTTGTGGGTGAGAAGAGGTTTGAGTCGATTCATGATCTGGTG
  ACAGATGGCTTGATAACACTGTACATAGAAACAAAAGCTGCCGAGTACATTTCAAAAATGACAACTAAC
  CCCATCTATGAACACATTGGATATGCCACCCTACTCAGAGAAAAAGTATCCAGAAGGCTGAGCAGGTCT
  AAAAATGAACCAAGAAAAACAAACGTCACACATGAAGAACACACAGCGGTGGAAAAGATCTCCTCCCTG
  GTTCGAAGGGCTGCCCTCACACACAACGACAACCACTTCAATTATGAGAAGACACACAACTTTAAGGTC
  CACACGTTCCGAGGCCCACACTGGTGTGAATATTGTGCCAATTTCATGTGGGGGCTCATCGCCCAAGGG
  GTCCGGTGCTCAGACTGTGGATTGAACGTACACAAACAGTGTTCCAAGCACGTTCCCAATGACTGCCAA
  CCTGATCTCAAGAGGATCAAGAAAGTGTACTGTTGTGACCTCACAACACTTGTGAAGGCTCACAACACT
  CAGAGACCCATGGTGGTAGACATATGCATTCGGGAAATTGAAGCAAGAGGATTAAAATCGGAAGGCCTT
  TACAGAGTCTCTGGGTTCACTGAACACATTGAAGATGTCAAAATGGCATTTGACAGAGATGGTGAAAAG
  GCCGATATATCTGCCAATGTCTATCCAGACATAAACATCATCACTGGAGCCCTTAAACTGTATTTCAGA
  GACTTACCCATCCCTGTCATCACATATGATACCTATTCCAAATTTATAGATGCAGCAAAAATCTCCAAT
  GCAGATGAGAGGCTGGAAGCCGTCCATGAAGTGCTGATGCTGCTGCCTCCTGCCCACTATGAAACCCTC
  CGGTACCTAATGATCCACCTCAAAAAGGTTACTATGAATGAAAAAGACAATTTCATGAATGCAGAAAAT
  CTGGGGATCGTGTTTGGGCCCACTCTGATGAGGCCCCCTGAGGACAGCACCCTGACCACCCTGCATGAT
  ATGCGGTACCAAAAGCTGATTGTGCAGATTTTAATAGAAAACGAAGACGTTTTATTC
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG238206
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MLKNTSLLYGNHTVENRPKYYGREFHGIISREQADELLGGVEGAYILRESQRQPGCYTLALRFGNQTLN
  YRLFHDGKHFVGEKRFESIHDLVTDGLITLYIETKAAEYISKMTTNPIYEHIGYATLLREKVSRRLSRS
  KNEPRKTNVTHEEHTAVEKISSLVRRAALTHNDNHFNYEKTHNFKVHTFRGPHWCEYCANFMWGLIAQG
  VRCSDCGLNVHKQCSKHVPNDCQPDLKRIKKVYCCDLTTLVKAHNTQRPMVVDICIREIEARGLKSEGL
  YRVSGFTEHIEDVKMAFDRDGEKADISANVYPDINIITGALKLYFRDLPIPVITYDTYSKFIDAAKISN
  ADERLEAVHEVLMLLPPAHYETLRYLMIHLKKVTMNEKDNFMNAENLGIVFGPTLMRPPEDSTLTTLHD
  MRYQKLIVQILIENEDVLF
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001293071
• ORF Size: 1299 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001293071.1, NP_001280000.1
• RefSeq Size: 3405 bp
• RefSeq ORF: 1302 bp
• Locus ID: 1124
• Cytogenetics: 7p14.3
• MW: 50.5 kDa
• Gene Summary: 'This gene encodes a guanosine triphosphate (GTP)-metabolizing protein that contains a phorbol-ester/diacylglycerol (DAG)-type zinc finger, a Rho-GAP domain, and an SH2 domain. The encoded protein translocates from the cytosol to the Golgi apparatus membrane upon binding by diacylglycerol (DAG). Activity of this protein is important in cell proliferation and migration, and expression changes in this gene have been detected in cancers. A mutation in this gene has also been associated with schizophrenia in men. Alternative transcript splicing and the use of alternative promoters results in multiple transcript variants. [provided by RefSeq, May 2014]'