CTP synthase (CTPS1) (NM_001301237) Human Tagged ORF Clone

CAT#: RG238218

CTPS1 (GFP-tagged) - Human CTP synthase 1 (CTPS1), transcript variant 2

Specifications

Product Data

• Tag: TurboGFP
• Symbol: CTPS1
• Synonyms: CTPS; GATD5; IMD24
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG238218 representing NM_001301237.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGCCCTTTATTGAGGCCTTCCGTCAGTTCCAATTCAAGGTCAAAAGAGAGAACTTTTGTAACATCCAC
  GTCAGTCTAGTTCCCCAGCCAAGTTCAACAGGGGAACAGAAGACTAAACCTACCCAGAATAGTGTTCGG
  GAACTTAGAGGACTTGGGCTTTCCCCAGATCTGGTTGTATGCAGGTGCTCAAATCCACTTGACACATCA
  GTGAAGGAGAAAATATCAATGTTCTGCCATGTTGAGCCTGAACAAGTGATCTGTGTCCACGATGTCTCA
  TCCATCTACCGAGTCCCCTTGTTGTTAGAGGAGCAAGGGGTTGTAGATTATTTTCTTCGAAGACTTGAC
  CTTCCTATTGAGAGGCAGCCAAGAAAAATGCTGATGAAATGGAAAGAGATGGCTGACAGATATGATCGC
  TTGCTGGAGACCTGCTCTATTGCCCTTGTGGGCAAATACACGAAGTTCTCAGACTCCTATGCCTCTGTC
  ATTAAGGCTCTGGAGCATTCTGCACTGGCCATCAACCACAAATTGGAAATCAAGTACATAGATTCTGCG
  GACTTGGAGCCCATCACCTCGCAAGAAGAGCCCGTGCGCTACCACGAAGCTTGGCAGAAGCTCTGTAGT
  GCTCATGGAGTGCTGGTTCCAGGAGGATTTGGTGTTCGAGGAACAGAAGGAAAAATCCAAGCAATTGCC
  TGGGCTCGGAATCAGAAAAAGCCTTTTTTGGGCGTGTGCTTAGGGATGCAGTTGGCAGTGGTTGAATTC
  TCAAGAAACGTGCTGGGATGGCAAGATGCCAATTCTACAGAGTTTGACCCTACGACCAGTCATCCCGTG
  GTCGTAGACATGCCAGAACACAACCCAGGGCAGATGGGCGGAACCATGAGGCTGGGCAAGAGGAGAACC
  CTGTTCCAGACCAAGAACTCAGTCATGAGGAAACTCTATGGAGACGCAGACTACTTGGAAGAGAGGCAC
  CGCCACCGATTTGAGGTGAATCCAGTCTGGAAAAAGTGTTTGGAAGAACAAGGCTTGAAGTTTGTTGGC
  CAAGATGTTGAAGGAGAGAGAATGGAAATTGTGGAGTTAGAAGATCATCCCTTTTTTGTTGGGGTTCAG
  TACCACCCTGAGTTCCTGTCCAGGCCTATCAAGCCCTCCCCACCATACTTTGGCCTCCTCCTGGCCTCT
  GTGGGGCGGCTCTCACATTACCTCCAGAAAGGCTGCAGGCTCTCACCCAGGGACACCTATAGTGACAGG
  AGTGGAAGCAGCTCCCCTGACTCTGAAATCACCGAACTGAAGTTTCCATCAATAAATCATGAC
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG238218
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MPFIEAFRQFQFKVKRENFCNIHVSLVPQPSSTGEQKTKPTQNSVRELRGLGLSPDLVVCRCSNPLDTS
  VKEKISMFCHVEPEQVICVHDVSSIYRVPLLLEEQGVVDYFLRRLDLPIERQPRKMLMKWKEMADRYDR
  LLETCSIALVGKYTKFSDSYASVIKALEHSALAINHKLEIKYIDSADLEPITSQEEPVRYHEAWQKLCS
  AHGVLVPGGFGVRGTEGKIQAIAWARNQKKPFLGVCLGMQLAVVEFSRNVLGWQDANSTEFDPTTSHPV
  VVDMPEHNPGQMGGTMRLGKRRTLFQTKNSVMRKLYGDADYLEERHRHRFEVNPVWKKCLEEQGLKFVG
  QDVEGERMEIVELEDHPFFVGVQYHPEFLSRPIKPSPPYFGLLLASVGRLSHYLQKGCRLSPRDTYSDR
  SGSSSPDSEITELKFPSINHD
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001301237
• ORF Size: 1305 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001301237.2
• RefSeq Size: 2412 bp
• RefSeq ORF: 1308 bp
• Locus ID: 1503
• Cytogenetics: 1p34.2
• Protein Pathways: Metabolic pathways, Pyrimidine metabolism
• MW: 50.1 kDa
• Gene Summary: 'This gene encodes an enzyme responsible for the catalytic conversion of UTP (uridine triphosphate) to CTP (cytidine triphospate). This reaction is an important step in the biosynthesis of phospholipids and nucleic acids. Activity of this proten is important in the immune system, and loss of function of this gene has been associated with immunodeficiency. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]'