NUDT12 (NM_001300741) Human Tagged ORF Clone

CAT#: RG238268

NUDT12 (GFP-tagged) - Human nudix (nucleoside diphosphate linked moiety X)-type motif 12 (NUDT12), transcript variant 2

Specifications

Product Data

• Tag: TurboGFP
• Symbol: NUDT12
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG238268 representing NM_001300741.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGTCTTCTGTAAAAAGAAGTCTGAAGCAAGAAATAGTTACTCAGTTTCACTGTTCAGCTGCTGAAGGA
  GATATTGCCAAGTTAACAGGAATACTCAGTCATTCTCCATCTCTTCTCAATGAAACTTCTGAAAATGGC
  TGGACTGCTTTAATGTGTGACAGATCAATTGTCAATAAATCAAGGCAGACTGCACTGGACATTGCTGTA
  TTTTGGGGTTATAAGCATATAGCTAATTTACTAGCTACTGCTAAAGGTGGGAAGAAGCCTTGGTTCCTA
  ACGAATGAAGTGGAAGAATGTGAAAATTATTTTAGCAAAACACTACTGGACCGGAAAAGTGAAAAGAGA
  AATAATTCTGACTGGCTGCTAGCTAAAGAAAGCCATCCAGCCACAGTTTTTATTCTTTTCTCAGATTTA
  AATCCCTTGGTTACTCTAGGTGGCAATAAAGAAAGTTTCCAACAGCCAGAAGTTAGGCTTTGTCAGCTG
  AACTACACAGATATAAAGGATTATTTGGCCCAGCCTGAGAAGATCACCTTGATTTTTCTTGGAGTAGAA
  CTTGAAATAAAAGACAAACTACTTAATTATGCTGGTGAAGTCCCGAGAGAGGAGGAAGATGGATTGGTT
  GCCTGGTTTGCTCTAGGTATAGATCCTATTGCTGCTGAAGAATTCAAGCAAAGACATGAAAATTGTTAC
  TTTCTTCATCCTCCTATGCCAGCCCTTCTGCAATTGAAAGAAAAAGAAGCTGGGGTTGTAGCTCAAGCA
  AGATCTGTTCTTGCCTGGCACAGTCGATACAAGTTTTGCCCAACCTGTGGAAATGCAACTAAAATTGAA
  GAAGGTGGCTATAAGAGATTATGTTTAAAAGAAGACTGTCCTAGTCTCAATGGCGTCCATAATACCTCA
  TACCCAAGAGTTGATCCAGTAGTAATCATGCAAGTTATTCATCCAGATGGGACCAAATGCCTTTTAGGC
  AGGCAGAAAAGATTTCCCCCAGGCATGTTTACTTGCCTTGCTGGATTTATTGAGCCTGGAGAGACAATA
  GAAGATGCTGTTAGGAGAGAAGTAGAAGAGGAAAGTGGAGTCAAAGTTGGCCATGTTCAGTATGTTGCT
  TGTCAACCATGGCCAATGCCTTCCTCCTTAATGATTGGTTGCTTAGCTCTAGCAGTGTCTACAGAAATT
  AAAGTTGACAAGAATGAAATAGAGGATGCCCGCTGGTTCACTAGAGAACAGGTCCTGGATGTTCTGACC
  AAAGGGAAGCAGCAGGCATTCTTTGTGCCACCAAGCCGAGCTATTGCACATCAATTAATCAAACACTGG
  ATTAGAATAAATCCTAATCTC
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG238268
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MSSVKRSLKQEIVTQFHCSAAEGDIAKLTGILSHSPSLLNETSENGWTALMCDRSIVNKSRQTALDIAV
  FWGYKHIANLLATAKGGKKPWFLTNEVEECENYFSKTLLDRKSEKRNNSDWLLAKESHPATVFILFSDL
  NPLVTLGGNKESFQQPEVRLCQLNYTDIKDYLAQPEKITLIFLGVELEIKDKLLNYAGEVPREEEDGLV
  AWFALGIDPIAAEEFKQRHENCYFLHPPMPALLQLKEKEAGVVAQARSVLAWHSRYKFCPTCGNATKIE
  EGGYKRLCLKEDCPSLNGVHNTSYPRVDPVVIMQVIHPDGTKCLLGRQKRFPPGMFTCLAGFIEPGETI
  EDAVRREVEEESGVKVGHVQYVACQPWPMPSSLMIGCLALAVSTEIKVDKNEIEDARWFTREQVLDVLT
  KGKQQAFFVPPSRAIAHQLIKHWIRINPNL
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001300741
• ORF Size: 1332 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001300741.2
• RefSeq Size: 3460 bp
• RefSeq ORF: 1335 bp
• Locus ID: 83594
• Cytogenetics: 5q21.2
• Protein Pathways: Nicotinate and nicotinamide metabolism
• MW: 50.5 kDa
• Gene Summary: Nucleotides are involved in numerous biochemical reactions and pathways within the cell as substrates, cofactors, and effectors. Nudix hydrolases, such as NUDT12, regulate the concentrations of individual nucleotides and of nucleotide ratios in response to changing circumstances (Abdelraheim et al., 2003 [PubMed 12790796]). [supplied by OMIM, Mar 2008]