LIPI (NM_001303000) Human Tagged ORF Clone

CAT#: RG238319

LIPI (GFP-tagged) - Human lipase, member I (LIPI), transcript variant deltaE7

Specifications

Product Data

• Tag: TurboGFP
• Symbol: LIPI
• Synonyms: CT17; LPDL; mPA-PLA1 beta; PLA1C; PRED5
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG238319 representing NM_001303000.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGAGAGTATACATTTTTCTTTGTTTGATGTGCTGGGTGAGATCTGATAATAAAAGACCATGCCTTGAA
  TTCTCTCAGCTAAGTGTAAAGGATTCCTTCAGAGATTTATTTATTCCGAGAATAGAGACCATTCTGATG
  ATGTATACAAGGAACAACCTAAACTGTGCTGAGCCACTGTTTGAACAAAATAACTCACTTAATGTTAAT
  TTCAACACACAAAAGAAAACAGTCTGGCTTATTCACGGATACAGACCAGTAGGCTCCATCCCATTATGG
  CTTCAGAACTTCGTAAGGATTTTGCTGAATGAAGAAGATATGAATGTAATTGTAGTAGACTGGAGCCGG
  GGTGCTACAACTTTTATTTATAATAGAGCAGTTAAAAACACCAGAAAAGTTGCTGTGAGTTTGAGTGTG
  CACATTAAAAATCTTTTGAAGCATGGTGCATCTCTTGACAATTTTCATTTCATAGGTGTGAGCTTAGGG
  GCTCATATCAGTGGATTTGTTGGAAAGATATTTCATGGTCAACTTGGAAGAATAACAGGTCTTGACCCT
  GCTGGGCCAAGGTTCTCCAGAAAACCACCATATAGCAGATTAGATTACACGGATGCAAAGTTTGTGGAT
  GTCATCCATTCTGACTCCAATGGTTTAGGCATTCAAGAGCCCTTGGGACATATAGATTTTTATCCAAAT
  GGAGGAAATAAACAACCTGGCTGTCCTAAATCAATTTTCTCAGGAATTCAATTCATTAAATGCAACCAC
  CAGAGAGCAGTTCACTTGTTCATGGCATCTTTAGAAACAAACTGCAATTTTATTTCATTTCCTTGTCGT
  TCATACAAAGATTACAAGACTAGCTTATGTGTGGACTGTGACTGTTTTAAGGAAAAATCATGTCCTCGG
  CTGGGTTATCAAGCCAAGCTATTTAAAGGTGTTTTAAAAGAAAGGATGGAAGGAAGACCTCTTAGGACC
  ACTGTGTTTTTGGATACAAGTGCCTATTATTTTGTTCTCAGTATAATTGTTCCAGATAAAACTATGATG
  GATGGCTCGTTTTCATTTAAATTATTAAATCAGCTTGGAATGATTGAAGAGCCAAGGCTTTATGAAAAG
  AACAAACCATTTTATAAACTTCAAGAAGTCAAGATTCTTGCTCAATTTTATAATGACTTTGTAAATATT
  TCAAGCATTGGTTTGACATATTTCCAGAGCTCAAATCTGCAGTGTTCCACATGCACATACAAGATCCAG
  AGTCTCATGTTAAAATCACTTACATACCCAGAAAGACCACCACTTTGCAGGTATAATATTGTACTTAAA
  GACAGAGAGGAAGTGTTTCTTAATCCAAACACATGTACACCAAAGAACACA
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG238319
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MRVYIFLCLMCWVRSDNKRPCLEFSQLSVKDSFRDLFIPRIETILMMYTRNNLNCAEPLFEQNNSLNVN
  FNTQKKTVWLIHGYRPVGSIPLWLQNFVRILLNEEDMNVIVVDWSRGATTFIYNRAVKNTRKVAVSLSV
  HIKNLLKHGASLDNFHFIGVSLGAHISGFVGKIFHGQLGRITGLDPAGPRFSRKPPYSRLDYTDAKFVD
  VIHSDSNGLGIQEPLGHIDFYPNGGNKQPGCPKSIFSGIQFIKCNHQRAVHLFMASLETNCNFISFPCR
  SYKDYKTSLCVDCDCFKEKSCPRLGYQAKLFKGVLKERMEGRPLRTTVFLDTSAYYFVLSIIVPDKTMM
  DGSFSFKLLNQLGMIEEPRLYEKNKPFYKLQEVKILAQFYNDFVNISSIGLTYFQSSNLQCSTCTYKIQ
  SLMLKSLTYPERPPLCRYNIVLKDREEVFLNPNTCTPKNT
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001303000
• ORF Size: 1362 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001303000.2
• RefSeq Size: 1591 bp
• RefSeq ORF: 1365 bp
• Locus ID: 149998
• Cytogenetics: 21q11.2
• Protein Families: Secreted Protein
• MW: 52.7 kDa
• Gene Summary: The protein encoded by this gene is a phospholipase that hydrolyzes phosphatidic acid to produce lysophosphatidic acid. Defects in this gene are a cause of susceptibility to familial hypertrigliceridemia. This gene is also expressed at high levels in Ewing family tumor cells. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]