ENT2 (SLC29A2) (NM_001300868) Human Tagged ORF Clone

CAT#: RG238332

SLC29A2 (GFP-tagged) - Human solute carrier family 29 (equilibrative nucleoside transporter), member 2 (SLC29A2), transcript variant 1

Specifications

Product Data

• Tag: TurboGFP
• Symbol: SLC29A2
• Synonyms: DER12; ENT2; HNP36
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG238332 representing NM_001300868.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGCGCGAGGAGACGCCCCGCGGGACAGCTACCACCTGGTCGGGATCAGCTTCTTCATCCTGGGGCTG
  GGCACCCTCCTTCCCTGGAACTTCTTCATCACCGCCATCCCGTACTTCCAGGCGCGACTGGCCGGGGCC
  GGCAACAGCACAGCCAGGATCCTGAGCACCAACCACACGGGTCCCGAGGATGCCTTCAACTTCAACAAT
  TGGGTGACGCTGCTGTCCCAGCTGCCCCTGCTGCTCTTCACCCTCCTCAACTCCTTCCTGTACCAGTGC
  GTCCCGGAGACGGTGCGCATTCTGGGCAGCCTGCTGGCCATACTGCTGCTCTTTGCCCTGACAGCAGCG
  CTGGTCAAGGTGGACATGAGCCCCGGACCCTTCTTCTCCATCACCATGGCCTCCGTCTGCTTCATCAAC
  TCCTTCAGTGCAGTCCTACAGGGCAGCCTCTTCGGGCAGCTGGGCACCATGCCCTCCACCTACAGCACC
  CTCTTCCTCAGCGGCCAGGGCCTGGCTGGGATCTTTGCTGCCCTTGCCATGCTCCTGTCCATGGCCAGT
  GGCGTGGACGCCGAGACCTCTGCCCTGGGGTACTTTATCACGCCCTGTGTGGGCATCCTCATGTCCATC
  GTGTGTTACCTGAGCCTGCCTCACCTGAAGTTTGCCCGCTACTACCTGGCCAATAAATCATCCCAGGCC
  CAAGCTCAGGAGCTGGAGACCAAAGCTGAGCTCCTCCAGTCTGATGAGAACGGGATTCCCAGTAGTCCC
  CAGAAAGTAGCTCTGACCCTGGATCTTGACCTGGAGAAGGAGCCGGAATCAGAGCCAGATGAGCCCCAG
  AAGCCAGGAAAACCTTCAGTCTTCACTGTCTTCCAGAAGATCTGGCTGACAGCGCTGTGCCTTGTGTTG
  GTCTTCACAGTCACCCTGTCCGTCTTCCCCGCCATCACAGCCATGGTGACCAGCTCCACCAGTCCTGGG
  AAGTGGAGTCAGTTCTTCAACCCCATCTGCTGCTTCCTCCTCTTCAACATCATGGACTGGCTGGGACGG
  AGCCTGACCTCTTACTTCCTGTGGCCAGACGAGGACAGCCGGCTGCTGCCCCTGCTGGTCTGCCTGCGG
  TTCCTGTTCGTGCCCCTCTTCATGCTGTGCCACGTGCCCCAGAGGTCCCGGCTGCCCATCCTCTTCCCA
  CAGGATGCCTACTTCATCACCTTCATGCTGCTCTTTGCCGTTTCTAATGGCTACCTGGTGTCCCTCACC
  ATGTGCCTGGCGCCCAGGCAGGTGCTGCCACACGAGAGGGAGGTGGCCGGCGCCCTCATGACCTTCTTC
  CTGGCCCTGGGACTTTCCTGTGGAGCCTCCCTCTCCTTCCTCTTCAAGGCGCTGCTC
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG238332
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MARGDAPRDSYHLVGISFFILGLGTLLPWNFFITAIPYFQARLAGAGNSTARILSTNHTGPEDAFNFNN
  WVTLLSQLPLLLFTLLNSFLYQCVPETVRILGSLLAILLLFALTAALVKVDMSPGPFFSITMASVCFIN
  SFSAVLQGSLFGQLGTMPSTYSTLFLSGQGLAGIFAALAMLLSMASGVDAETSALGYFITPCVGILMSI
  VCYLSLPHLKFARYYLANKSSQAQAQELETKAELLQSDENGIPSSPQKVALTLDLDLEKEPESEPDEPQ
  KPGKPSVFTVFQKIWLTALCLVLVFTVTLSVFPAITAMVTSSTSPGKWSQFFNPICCFLLFNIMDWLGR
  SLTSYFLWPDEDSRLLPLLVCLRFLFVPLFMLCHVPQRSRLPILFPQDAYFITFMLLFAVSNGYLVSLT
  MCLAPRQVLPHEREVAGALMTFFLALGLSCGASLSFLFKALL
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001300868
• ORF Size: 1368 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001300868.1, NP_001287797.1
• RefSeq Size: 2730 bp
• RefSeq ORF: 1371 bp
• Locus ID: 3177
• Cytogenetics: 11q13.2
• Protein Families: Transmembrane
• MW: 50.1 kDa
• Gene Summary: 'The uptake of nucleosides by transporters, such as SLC29A2, is essential for nucleotide synthesis by salvage pathways in cells that lack de novo biosynthetic pathways. Nucleoside transport also plays a key role in the regulation of many physiologic processes through its effect on adenosine concentration at the cell surface (Griffiths et al., 1997 [PubMed 9396714]).[supplied by OMIM, Nov 2008]'