SMAP1 (NM_001281440) Human Tagged ORF Clone

CAT#: RG238340

SMAP1 (GFP-tagged) - Human small ArfGAP 1 (SMAP1), transcript variant 4

Specifications

Product Data

• Tag: TurboGFP
• Symbol: SMAP1
• Synonyms: SMAP-1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG238340 representing NM_001281440.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGAGCCCTACCTGCCTGCCCACCTGACAGCTTTTGTACAAGGCTTTTATCTCTGCTTCCTGAAAAAG
  AGTATGGTGGTCTTTTTCGGTCCTCGATGGGCTTCCTGGAATATTGGTGTGTTTATTTGCATCAGATGT
  GCTGGAATTCATAGAAATCTTGGGGTTCATATATCCAGGGTCAAATCAGTCAACCTAGACCAATGGACA
  GCAGAACAGATACAGTGCATGCAAGATATGGGAAATACTAAAGCAAGACTACTCTATGAAGCCAATCTT
  CCAGAGAACTTTCGAAGACCACAGACAGATCAAGCAGTGGAATTTTTCATCAGAGATAAATATGAAAAG
  AAGAAATACTACGATAAAAATGCCATAGCTATTACAAATATTTCCTCCTCTGATGCTCCTCTTCAGCCT
  TTGGTATCCTCTCCTTCTCTGCAAGCTGCTGTTGACAAAAATAAATTGGAGAAAGAAAAGGAAAAAAAA
  AAGGAAGAGAAAAAGAGAGAAAAGGAGCCAGAAAAGCCGGCAAAACCACTTACAGCTGAAAAGCTGCAG
  AAGAAAGATCAGCAACTGGAGCCTAAAAAAAGTACCAGCCCTAAAAAAGCTGCGGAGCCCACTGTGGAT
  CTTTTAGGACTTGATGGCCCTGCTGTGGCACCAGTGACCAACGGGAACACAACGGTGCCACCCCTGAAC
  GATGATCTGGACATCTTTGGACCGATGATTTCTAATCCCTTACCTGCAACTGTCATGCCCCCAGCTCAG
  GGGACACCCTCTGCACCAGCAGCTGCAACCCTGTCTACAGTAACATCTGGGGATCTAGATTTATTCACT
  GAGCAAACTACAAAATCAGAAGAAGTGGCAAAGAAACAACTTTCCAAAGACTCCATCTTATCTCTGTAT
  GGCACAGGAACCATTCAACAGCAAAGTACTCCTGGTGTATTTATGGGACCCACAAATATACCATTTACC
  TCACAAGCACCAGCTGCATTTCAGGGCTTTCCATCGATGGGCGTGCCTGTGCCTGCAGCTCCTGGCCTT
  ATAGGAAATGTGATGGGACAGAGTCCAAGCATGATGGTGGGCATGCCCATGCCCAATGGGTTTATGGGA
  AATGCACAAACTGGTGTGATGCCACTTCCTCAGAACGTTGTTGGCCCCCAAGGAGGAATGGTGGGACAA
  ATGGGTGCACCCCAGAGTAAGTTTGGCCTGCCGCAAGCTCAGCAGCCCCAGTGGAGCCTCTCACAGATG
  AATCAGCAGATGGCTGGCATGAGTATCAGTAGTGCAACCCCTACTGCAGGTTTTGGCCAGCCCTCCAGC
  ACAACAGCAGGATGGTCTGGAAGCTCATCAGGTCAGACTCTCAGCACACAACTGTGGAAA
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG238340
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MEPYLPAHLTAFVQGFYLCFLKKSMVVFFGPRWASWNIGVFICIRCAGIHRNLGVHISRVKSVNLDQWT
  AEQIQCMQDMGNTKARLLYEANLPENFRRPQTDQAVEFFIRDKYEKKKYYDKNAIAITNISSSDAPLQP
  LVSSPSLQAAVDKNKLEKEKEKKKEEKKREKEPEKPAKPLTAEKLQKKDQQLEPKKSTSPKKAAEPTVD
  LLGLDGPAVAPVTNGNTTVPPLNDDLDIFGPMISNPLPATVMPPAQGTPSAPAAATLSTVTSGDLDLFT
  EQTTKSEEVAKKQLSKDSILSLYGTGTIQQQSTPGVFMGPTNIPFTSQAPAAFQGFPSMGVPVPAAPGL
  IGNVMGQSPSMMVGMPMPNGFMGNAQTGVMPLPQNVVGPQGGMVGQMGAPQSKFGLPQAQQPQWSLSQM
  NQQMAGMSISSATPTAGFGQPSSTTAGWSGSSSGQTLSTQLWK
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001281440
• ORF Size: 1371 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001281440.1, NP_001268369.1
• RefSeq Size: 3165 bp
• RefSeq ORF: 1374 bp
• Locus ID: 60682
• Cytogenetics: 6q13
• Protein Pathways: Endocytosis
• MW: 49.7 kDa
• Gene Summary: The protein encoded by this gene is similar to the mouse stromal membrane-associated protein-1. This similarity suggests that this human gene product is also a type II membrane glycoprotein involved in the erythropoietic stimulatory activity of stromal cells. Alternate splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]