XRN1 (NM_001282859) Human Tagged ORF Clone

CAT#: RG238350

XRN1 (GFP-tagged) - Human 5'-3' exoribonuclease 1 (XRN1), transcript variant 4

Specifications

Product Data

• Tag: TurboGFP
• Symbol: XRN1
• Synonyms: SEP1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG238350 representing NM_001282859.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGGAGTCCCCAAGTTTTACAGATGGATCTCAGAGCGGTATCCCTGTCTCAGCGAAGTGGTGAAAGAG
  CATCAGATTCCTGAATTTGACAACTTGTACCTGGATATGAATGGAATTATACATCAGTGCTCCCATCCT
  AATGATGATGATGTTCACTTTAGAATTTCAGATGATAAAATCTTTACTGATATTTTTCACTACCTGGAG
  GTGTTGTTTCGCATTATTAAACCCAGGAAAGTGTTCTTTATGGCTGTAGATGGTGTGGCTCCTCGAGCA
  AAAATGAACCAGCAGCGTGGGAGGCGTTTTAGGTCAGCAAAGGAGGCAGAAGACAAAATTAAAAAGGCA
  ATAGAGAAGGGAGAAACTCTTCCTACAGAGGCCAGATTTGATTCCAACTGTATCACACCAGGAACTGAA
  TTTATGGCCAGGTTACATGAACATCTGAAGTATTTTGTAAATATGAAAATTTCCACAGACAAGTCATGG
  CAAGGAGTTACCATCTACTTCTCAGGCCATGAGACTCCTGGAGAAGGAGAGCATAAAATCATGGAATTT
  ATCAGATCCGAGAAAGCAAAGCCAGATCATGATCCAAACACCAGACACTGTCTTTATGGTTTAGATGCT
  GACTTGATTATGCTTGGATTAACAAGTCATGAGGCACATTTTTCTCTCTTAAGAGAAGAAGTTCGATTT
  GGTGGCAAAAAAACACAACGGGTATGTGCTCCAGAAGAAACTACATTTCACCTTCTACACTTGTCTTTA
  ATGAGAGAGTATATTGACTATGAGTTTTCAGTATTAAAAGAAAAGATCACATTTAAATATGATATTGAA
  AGGATAATAGATGATTGGATTTTGATGGGGTTTCTTGTTGGTAATGATTTTATCCCTCATCTACCTCAT
  TTACATATTAATCATGATGCACTGCCTCTTCTTTATGGAACATATGTTACCATCCTGCCAGAACTTGGG
  GGTTATATTAATGAAAGTGGGCACCTCAACTTACCTCGATTTGAGAAATACCTTGTGAAACTATCAGAT
  TTTGATCGGGAGCACTTCAGTGAAGTTTTTGTGGACCTAAAATGGTTTGAAAGCAAAGTTGGTAACAAG
  TACCTCAATGAAGCAGCAGGTGTCGCAGCAGAAGAAGCCAGGAACTACAAGGAAAAGAAAAAGTTAAAG
  GGCCAGGAAAATTCTCTGTGTTGGACTGCTTTAGACAAAAATGAAGGCGAAATGATAACTTCTAAGGAT
  AATTTAGAAGATGAGACTGAAGATGATGACCTATTTGAAACTGAGTTTAGACAATATAAAAGAACATAT
  TACATGACGAAGATGGGGGTTGACGTAGTATCTGAGTACGTTTTTGCCAATGCTTTTATTTTAAAA
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG238350
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MGVPKFYRWISERYPCLSEVVKEHQIPEFDNLYLDMNGIIHQCSHPNDDDVHFRISDDKIFTDIFHYLE
  VLFRIIKPRKVFFMAVDGVAPRAKMNQQRGRRFRSAKEAEDKIKKAIEKGETLPTEARFDSNCITPGTE
  FMARLHEHLKYFVNMKISTDKSWQGVTIYFSGHETPGEGEHKIMEFIRSEKAKPDHDPNTRHCLYGLDA
  DLIMLGLTSHEAHFSLLREEVRFGGKKTQRVCAPEETTFHLLHLSLMREYIDYEFSVLKEKITFKYDIE
  RIIDDWILMGFLVGNDFIPHLPHLHINHDALPLLYGTYVTILPELGGYINESGHLNLPRFEKYLVKLSD
  FDREHFSEVFVDLKWFESKVGNKYLNEAAGVAAEEARNYKEKKKLKGQENSLCWTALDKNEGEMITSKD
  NLEDETEDDDLFETEFRQYKRTYYMTKMGVDVVSEYVFANAFILK
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001282859
• ORF Size: 1377 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001282859.1, NP_001269788.1
• RefSeq Size: 2152 bp
• RefSeq ORF: 1380 bp
• Locus ID: 54464
• Cytogenetics: 3q23
• Protein Pathways: RNA degradation
• MW: 54.3 kDa
• Gene Summary: This gene encodes a member of the 5'-3' exonuclease family. The encoded protein may be involved in replication-dependent histone mRNA degradation, and interacts directly with the enhancer of mRNA-decapping protein 4. In addition to mRNA metabolism, a similar protein in yeast has been implicated in a variety of nuclear and cytoplasmic functions, including homologous recombination, meiosis, telomere maintenance, and microtubule assembly. Mutations in this gene are associated with osteosarcoma, suggesting that the encoded protein may also play a role in bone formation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]