PLAGL1 (NM_001289046) Human Tagged ORF Clone

CAT#: RG238380

PLAGL1 (GFP-tagged) - Human pleiomorphic adenoma gene-like 1 (PLAGL1), transcript variant 17

Specifications

Product Data

• Tag: TurboGFP
• Symbol: PLAGL1
• Synonyms: LOT1; ZAC; ZAC1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG238380 representing NM_001289046.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGCCACGTTCCCCTGCCAGTTATGTGGCAAGACGTTCCTCACCCTGGAGAAGTTCACGATTCACAAT
  TATTCCCACTCCAGGGAGCGGCCGTACAAGTGTGTGCAGCCTGACTGTGGCAAAGCCTTTGTTTCCAGA
  TATAAATTGATGAGGCATATGGCTACCCATTCTCCCCAGAAATCTCACCAGTGTGCTCACTGTGAGAAG
  ACGTTCAACCGGAAAGACCACCTGAAAAACCACCTCCAGACCCACGACCCCAACAAAATGGCCTTTGGG
  TGTGAGGAGTGTGGGAAGAAGTACAACACCATGCTGGGCTATAAGAGGCACCTGGCCCTCCATGCGGCC
  AGCAGTGGGGACCTCACCTGTGGGGTCTGTGCCCTGGAGCTAGGGAGCACCGAGGTGCTACTGGACCAC
  CTCAAAGCCCATGCGGAAGAGAAGCCCCCTAGCGGAACCAAGGAAAAGAAGCACCAGTGCGACCACTGT
  GAAAGATGCTTCTACACCCGGAAGGATGTGCGACGCCACCTGGTGGTCCACACAGGATGCAAGGACTTC
  CTGTGCCAGTTCTGTGCCCAGAGATTTGGGCGCAAGGATCACCTCACCCGGCATACCAAGAAGACCCAC
  TCACAGGAGCTGATGAAAGAGAGCTTGCAGACCGGAGACCTTCTGAGCACCTTCCACACCATCTCGCCT
  TCATTCCAACTGAAGGCTGCTGCCTTGCCTCCTTTCCCTTTAGGAGCTTCTGCCCAGAACGGGCTTGCA
  AGTAGCTTGCCAGCTGAGGTCCATAGCCTCACCCTCAGTCCCCCAGAACAAGCCGCCCAGCCTATGCAG
  CCGCTGCCAGAGTCCCTGGCCTCCCTCCACCCCTCGGTATCCCCTGGCTCTCCTCCGCCACCCCTTCCC
  AATCACAAGTACAACACCACTTCTACCTCATACTCCCCACTTGCAAGCCTGCCCCTCAAAGCAGATACT
  AAAGGTTTTTGCAATATCAGTTTGTTTGAGGACTTGCCTCTGCAAGAGCCTCAGTCACCTCAAAAGCTC
  AACCCAGGTTTTGATCTGGCTAAGGGAAATGCTGGTAAAGTAAACCTGCCCAAGGAGCTGCCTGCAGAT
  GCTGTGAACCTAACAATACCTGCCTCTCTGGACCTGTCCCCCCTGTTGGGCTTCTGGCAGCTGCCCCCT
  CCTGCTACCCAAAATACCTTTGGGAATAGCACTCTTGCCCTGGGGCCTGGGGAATCTTTGCCCCACAGG
  TTAAGCTGTCTGGGGCAGCAGCAGCAAGAACCCCCACTTGCCATGGGCACTGTGAGCCTGGGCCAGCTC
  CCCCTGCCCCCCATCCCTCATGTGTTCTCAGCTGGCACTGGCTCTGCCATCCTGCCTCATTTCCATCAT
  GCATTCAGA
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG238380
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MATFPCQLCGKTFLTLEKFTIHNYSHSRERPYKCVQPDCGKAFVSRYKLMRHMATHSPQKSHQCAHCEK
  TFNRKDHLKNHLQTHDPNKMAFGCEECGKKYNTMLGYKRHLALHAASSGDLTCGVCALELGSTEVLLDH
  LKAHAEEKPPSGTKEKKHQCDHCERCFYTRKDVRRHLVVHTGCKDFLCQFCAQRFGRKDHLTRHTKKTH
  SQELMKESLQTGDLLSTFHTISPSFQLKAAALPPFPLGASAQNGLASSLPAEVHSLTLSPPEQAAQPMQ
  PLPESLASLHPSVSPGSPPPPLPNHKYNTTSTSYSPLASLPLKADTKGFCNISLFEDLPLQEPQSPQKL
  NPGFDLAKGNAGKVNLPKELPADAVNLTIPASLDLSPLLGFWQLPPPATQNTFGNSTLALGPGESLPHR
  LSCLGQQQQEPPLAMGTVSLGQLPLPPIPHVFSAGTGSAILPHFHHAFR
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001289046
• ORF Size: 1389 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001289046.2
• RefSeq Size: 3690 bp
• RefSeq ORF: 1392 bp
• Locus ID: 5325
• Cytogenetics: 6q24.2
• Protein Families: Transcription Factors
• MW: 50.8 kDa
• Gene Summary: 'This gene encodes a C2H2 zinc finger protein that functions as a suppressor of cell growth. This gene is often deleted or methylated and silenced in cancer cells. In addition, overexpression of this gene during fetal development is thought to be the causal factor for transient neonatal diabetes mellitus (TNDM). Alternative splicing and the use of alternative promoters results in multiple transcript variants encoding two different protein isoforms. The P1 downstream promoter of this gene is imprinted, with preferential expression from the paternal allele in many tissues. [provided by RefSeq, Nov 2015]'