C2 (NM_001282457) Human Tagged ORF Clone

CAT#: RG238614

C2 (GFP-tagged) - Human complement component 2 (C2), transcript variant 4

Specifications

Product Data

• Tag: TurboGFP
• Symbol: C2
• Synonyms: ARMD14; CO2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG238614 representing NM_001282457.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGGGACAAGGTCCGCTATCGCTGCTCCTCGAATCTTGTGCTCACGGGGTCTTCGGAGCGGGAGTGCC
  AGGGCAACGGGGTCTGGAGTGGAACGGAGCCCATCTGCCGCCATCTTCAGCTTTGAGATCAATGTGAGC
  GTTGCCATTATCACCTTTGCCTCAGAGCCCAAAGTCCTCATGTCTGTCCTGAACGACAACTCCCGGGAT
  ATGACTGAGGTGATCAGCAGCCTGGAAAATGCCAACTATAAAGATCATGAAAATGGAACTGGGACTAAC
  ACCTATGCGGCCTTAAACAGTGTCTATCTCATGATGAACAACCAAATGCGACTCCTCGGCATGGAAACG
  ATGGCCTGGCAGGAAATCCGACATGCCATCATCCTTCTGACAGATGGAAAGTCCAATATGGGTGGCTCT
  CCCAAGACAGCTGTTGACCATATCAGAGAGATCCTGAACATCAACCAGAAGAGGAATGACTATCTGGAC
  ATCTATGCCATCGGGGTGGGCAAGCTGGATGTGGACTGGAGAGAACTGAATGAGCTAGGGTCCAAGAAG
  GATGGTGAGAGGCATGCCTTCATTCTGCAGGACACAAAGGCTCTGCACCAGGTCTTTGAACATATGCTG
  GATGTCTCCAAGCTCACAGACACCATCTGCGGGGTGGGGAACATGTCAGCAAACGCCTCTGACCAGGAG
  AGGACACCCTGGCATGTCACTATTAAGCCCAAGAGCCAAGAGACCTGCCGGGGGGCCCTCATCTCCGAC
  CAATGGGTCCTGACAGCAGCTCATTGCTTCCGCGATGGCAACGACCACTCCCTGTGGAGGGTCAATGTG
  GGAGACCCCAAATCCCAGTGGGGCAAAGAATTCCTTATTGAGAAGGCGGTGATCTCCCCAGGGTTTGAT
  GTCTTTGCCAAAAAGAACCAGGGAATCCTGGAGTTCTATGGTGATGACATAGCTCTGCTGAAGCTGGCC
  CAGAAAGTAAAGATGTCCACCCATGCCAGGCCCATCTGCCTTCCCTGCACGATGGAGGCCAATCTGGCT
  CTGCGGAGACCTCAAGGCAGCACCTGTAGGGACCATGAGAATGAACTGCTGAACAAACAGAGTGTTCCT
  GCTCATTTTGTCGCCTTGAATGGGAGCAAACTGAACATTAACCTTAAGATGGGAGTGGAGTGGACAAGC
  TGTGCCGAGGTTGTCTCCCAAGAAAAAACCATGTTCCCCAACTTGACAGATGTCAGGGAGGTGGTGACA
  GACCAGTTCCTATGCAGTGGGACCCAGGAGGATGAGAGTCCCTGCAAGGGAGAATCTGGGGGAGCAGTT
  TTCCTTGAGCGGAGATTCAGGTTTTTTCAGGTGGGTCTGGTGAGCTGGGGTCTTTACAACCCCTGCCTT
  GGCTCTGCTGACAAAAACTCCCGCAAAAGGGCCCCTCGTAGCAAGGTCCCGCCGCCACGAGACTTTCAC
  ATCAATCTCTTCCGCATGCAGCCCTGGCTGAGGCAGCACCTGGGGGATGTCCTGAATTTTTTACCCCTC
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG238614
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MGTRSAIAAPRILCSRGLRSGSARATGSGVERSPSAAIFSFEINVSVAIITFASEPKVLMSVLNDNSRD
  MTEVISSLENANYKDHENGTGTNTYAALNSVYLMMNNQMRLLGMETMAWQEIRHAIILLTDGKSNMGGS
  PKTAVDHIREILNINQKRNDYLDIYAIGVGKLDVDWRELNELGSKKDGERHAFILQDTKALHQVFEHML
  DVSKLTDTICGVGNMSANASDQERTPWHVTIKPKSQETCRGALISDQWVLTAAHCFRDGNDHSLWRVNV
  GDPKSQWGKEFLIEKAVISPGFDVFAKKNQGILEFYGDDIALLKLAQKVKMSTHARPICLPCTMEANLA
  LRRPQGSTCRDHENELLNKQSVPAHFVALNGSKLNINLKMGVEWTSCAEVVSQEKTMFPNLTDVREVVT
  DQFLCSGTQEDESPCKGESGGAVFLERRFRFFQVGLVSWGLYNPCLGSADKNSRKRAPRSKVPPPRDFH
  INLFRMQPWLRQHLGDVLNFLPL
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001282457
• ORF Size: 1518 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001282457.2
• RefSeq Size: 2069 bp
• RefSeq ORF: 1521 bp
• Locus ID: 717
• Cytogenetics: 6p21.33
• Protein Families: Druggable Genome, Protease, Secreted Protein
• Protein Pathways: Complement and coagulation cascades, Systemic lupus erythematosus
• MW: 56.9 kDa
• Gene Summary: 'Component C2 is a serum glycoprotein that functions as part of the classical pathway of the complement system. Activated C1 cleaves C2 into C2a and C2b. The serine proteinase C2a then combines with complement factor 4b to create the C3 or C5 convertase. Deficiency of C2 has been reported to associated with certain autoimmune diseases and SNPs in this gene have been associated with altered susceptibility to age-related macular degeneration. This gene localizes within the class III region of the MHC on the short arm of chromosome 6. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described in publications but their full-length sequence has not been determined.[provided by RefSeq, Mar 2009]'