EYA4 (NM_001301012) Human Tagged ORF Clone

CAT#: RG238989

EYA4 (GFP-tagged) - Human EYA transcriptional coactivator and phosphatase 4 (EYA4), transcript variant 5

Specifications

Product Data

• Tag: TurboGFP
• Symbol: EYA4
• Synonyms: CMD1J; DFNA10
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG238989 representing NM_001301012.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGAAGACTCCCAGGATTTAAATGAACAATCAGTAAAGAAAACGTGCACAGAATCAGATGTTTCACAA
  TCTCAGAATTCCAGGTCTATGGAAATGCAGGACCTAGCAAGTCCTCATACTCTTGTTGGAGGTGGTGAT
  ACTCCAGGTAGCTCCAAACTGGAAAAATCTAATCTCAGCAGCACATCAGTTACTACAAATGGGACAGGA
  GTAATTACAAGTAGTGGCTACAGCCCCAGATCAGCACATCAGTATTCCCCACAGCTGTATCCTTCCAAG
  CCCTATCCACACATTCTTTCTACACCAGCAGCTCAAACAATGTCTGCCTATGCAGGCCAGACTCAGTAT
  TCGGGGATGCAGCAGCCAGCCGTCTACACAGCCTACTCACAGACAGGACAGCCCTACAGCTTGCCCACT
  TACGATTTGGGTGTGATGTTGCCAGCCATCAAGACAGAGAGTGGACTTTCCCAAACTCAGTCCCCATTA
  CAGAGTGGCTGCCTCAGTTACAGCCCAGGGTTCTCTACCCCACAGCCAGGCCAGACACCTTATTCTTAC
  CAAATGCCAGGTTCTAGTTTTGCACCATCATCTACTATTTATGCAAATAATTCAGTTTCCAATTCAACG
  AATTTCAGTGGTTCACAACAGGATTATCCATCCTATACAGCCTTTGGCCAAAACCAGTATGCACAGTAT
  TATTCAGCATCAACGTATGGAGCGTATATGACATCGAATAACACAGCCGATGGCACACCCTCTTCAACC
  TCTACTTATCAGTTGCAGGAATCTCTCCCAGGACTGACTAACCAACCAGGAGAGTTCGATACCATGCAG
  AGTCCCTCCACACCCATCAAAGATCTTGATGAGAGAACCTGTAGGAGTTCTGGGTCAAAGTCCAGAGGA
  AGAGGCCGGAAAAATAATCCCTCCCCGCCTCCTGATAGTGACCTGGAGCGTGTGTTTGTCTGGGATTTG
  GATGAAACCATCATTGTTTTTCACTCACTGCTCACCGGGTCTTATGCACAGAAGTATGGCAAGGATCCC
  CCCATGGCTGTAACCCTTGGACTCCGCATGGAAGAAATGATTTTTAATCTTGCTGATACTCATTTGTTT
  TTTAATGATTTAGAGGAGTGTGATCAAGTTCATATAGATGATGTTTCCTCTGATGATAATGGGCAGGAC
  TTAAGTACCTACAGTTTTGCAACTGATGGCTTCCATGCAGCTGCAAGTAGTGCAAACCTTTGTTTGCCA
  ACAGGTGTAAGAGGAGGGGTTGACTGGATGAGGAAGTTGGCTTTTCGTTACAGAAGAGTAAAAGAATTA
  TATAACACCTACAAGAACAACGTTGGAGGACTCCTTGGCCCTGCCAAGAGGGATGCCTGGCTACAGTTA
  AGGGCAGAGATTGAAGGTCTGACAGATTCCTGGCTAACAAATGCACTTAAGTCTTTATCAATTATTAGC
  ACTAGGAGTAACTGCATAAATGTCTTGGTAACGACAACTCAACTGATCCCAGCACTTGCGAAGGTTCTA
  CTCTATAGTTTAGGAGGTGCTTTCCCCATTGAGAATATTTACAGTGCAACTAAAATAGGCAAGGAAAGC
  TGTTTTGAGCGTATAGTGTCCAGATTTGGCACTAACATAACTTATGTTGTGATTGGAGATGGCCGAGAT
  GAGGAGCATGCCGCTAACCAGCACAACATGCCCTTCTGGAGGATATCCAGTCACTCAGACCTCCTGGCT
  CTCCACCAAGCACTGGAATTAGAGTATTTG
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG238989
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MEDSQDLNEQSVKKTCTESDVSQSQNSRSMEMQDLASPHTLVGGGDTPGSSKLEKSNLSSTSVTTNGTG
  VITSSGYSPRSAHQYSPQLYPSKPYPHILSTPAAQTMSAYAGQTQYSGMQQPAVYTAYSQTGQPYSLPT
  YDLGVMLPAIKTESGLSQTQSPLQSGCLSYSPGFSTPQPGQTPYSYQMPGSSFAPSSTIYANNSVSNST
  NFSGSQQDYPSYTAFGQNQYAQYYSASTYGAYMTSNNTADGTPSSTSTYQLQESLPGLTNQPGEFDTMQ
  SPSTPIKDLDERTCRSSGSKSRGRGRKNNPSPPPDSDLERVFVWDLDETIIVFHSLLTGSYAQKYGKDP
  PMAVTLGLRMEEMIFNLADTHLFFNDLEECDQVHIDDVSSDDNGQDLSTYSFATDGFHAAASSANLCLP
  TGVRGGVDWMRKLAFRYRRVKELYNTYKNNVGGLLGPAKRDAWLQLRAEIEGLTDSWLTNALKSLSIIS
  TRSNCINVLVTTTQLIPALAKVLLYSLGGAFPIENIYSATKIGKESCFERIVSRFGTNITYVVIGDGRD
  EEHAANQHNMPFWRISSHSDLLALHQALELEYL
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001301012
• ORF Size: 1755 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001301012.1, NP_001287941.1
• RefSeq Size: 5393 bp
• RefSeq ORF: 1758 bp
• Locus ID: 2070
• Cytogenetics: 6q23.2
• Protein Families: Druggable Genome, Phosphatase, Transcription Factors
• MW: 64.4 kDa
• Gene Summary: This gene encodes a member of the eyes absent (EYA) family of proteins. The encoded protein may act as a transcriptional activator through its protein phosphatase activity, and it may be important for eye development, and for continued function of the mature organ of Corti. Mutations in this gene are associated with postlingual, progressive, autosomal dominant hearing loss at the deafness, autosomal dominant non-syndromic sensorineural 10 locus. The encoded protein is also a putative oncogene that mediates DNA repair, apoptosis, and innate immunity following DNA damage, cellular damage, and viral attack. Defects in this gene are also associated with dilated cardiomyopathy 1J. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2014]