DNA polymerase eta (POLH) (NM_001291969) Human Tagged ORF Clone

CAT#: RG239004

POLH (GFP-tagged) - Human polymerase (DNA directed), eta (POLH), transcript variant 2

Specifications

Product Data

• Tag: TurboGFP
• Symbol: POLH
• Synonyms: RAD30; RAD30A; XP-V; XPV
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG239004 representing NM_001291969.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGAAGCTCGTGCATTTGGAGTCACTAGAAGTATGTGGGCAGATGATGCTAAGAAGTTATGTCCAGATC
  TTCTACTGGCACAAGTTCGTGAGTCCCGTGGGAAAGCTAACCTCACCAAAGGGGATGCGAAAACAAGGC
  TTATTTCAATGGCTCGATTCTCTTCAGATTGATAACCTCACCTCTCCAGACCTGCAGCTCACCGTGGGA
  GCAGTGATTGTGGAGGAAATGAGAGCAGCCATAGAGAGGGAGACTGGTTTTCAGTGTTCAGCTGGAATT
  TCACACAATAAGGTCCTGGCAAAACTGGCCTGTGGACTAAACAAGCCCAACCGCCAAACCCTGGTTTCA
  CATGGGTCAGTCCCACAGCTCTTCAGCCAAATGCCCATTCGCAAAATCCGTAGTCTTGGAGGAAAGCTA
  GGGGCCTCTGTCATTGAGATCCTAGGGATAGAATACATGGGTGAACTGACCCAGTTCACTGAATCCCAG
  CTCCAGAGTCATTTTGGGGAGAAGAATGGGTCTTGGCTATATGCCATGTGCCGAGGGATTGAACATGAT
  CCAGTTAAACCCAGGCAACTACCCAAAACCATTGGCTGTAGTAAGAACTTCCCAGGAAAAACAGCTCTT
  GCTACTCGGGAACAGGTACAATGGTGGCTGTTGCAATTAGCCCAGGAACTAGAGGAGAGACTGACTAAA
  GACCGAAATGATAATGACAGGGTAGCCACCCAGCTGGTTGTGAGCATTCGTGTACAAGGAGACAAACGC
  CTCAGCAGCCTGCGCCGCTGCTGTGCCCTTACCCGCTATGATGCTCACAAGATGAGCCATGATGCATTT
  ACTGTCATCAAGAACTGTAATACTTCTGGAATCCAGACAGAATGGTCTCCTCCTCTCACAATGCTTTTC
  CTCTGTGCTACAAAATTTTCTGCCTCTGCCCCTTCATCTTCTACAGACATCACCAGCTTCTTGAGCAGT
  GACCCAAGTTCTCTGCCAAAGGTGCCAGTTACCAGCTCAGAAGCTAAGACCCAGGGAAGTGGCCCAGCG
  GTGACAGCCACTAAGAAAGCAACCACGTCTCTGGAATCATTCTTCCAAAAAGCTGCAGAAAGGCAGAAA
  GTTAAAGAAGCTTCGCTTTCATCTCTTACTGCTCCCACTCAGGCTCCCATGAGCAATTCACCATCCAAG
  CCCTCATTACCTTTTCAAACCAGTCAAAGTACAGGAACTGAGCCCTTCTTTAAGCAGAAAAGTCTGCTT
  CTAAAGCAGAAACAGCTTAATAATTCTTCAGTTTCTTCCCCCCAACAAAACCCATGGTCCAACTGTAAA
  GCATTACCAAACTCTTTACCAACAGAGTATCCAGGGTGTGTCCCTGTTTGTGAAGGGGTGTCGAAGCTA
  GAAGAATCCTCTAAAGCAACTCCTGCAGAGATGGATTTGGCCCACAACAGCCAAAGCATGCACGCCTCT
  TCAGCTTCCAAATCTGTGCTGGAGGTGACTCAGAAAGCAACCCCAAATCCAAGTCTTCTAGCTGCTGAG
  GACCAAGTGCCCTGTGAGAAGTGTGGCTCCCTGGTACCGGTATGGGATATGCCAGAACACATGGACTAT
  CATTTTGCATTGGAGTTGCAGAAATCCTTTTTGCAGCCCCACTCTTCAAACCCCCAGGTTGTTTCTGCC
  GTATCTCATCAAGGCAAAAGAAATCCCAAGAGCCCTTTGGCCTGCACTAATAAACGCCCCAGGCCTGAG
  GGCATGCAAACATTGGAATCATTTTTTAAGCCATTAACACAT
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG239004
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MKLVHLESLEVCGQMMLRSYVQIFYWHKFVSPVGKLTSPKGMRKQGLFQWLDSLQIDNLTSPDLQLTVG
  AVIVEEMRAAIERETGFQCSAGISHNKVLAKLACGLNKPNRQTLVSHGSVPQLFSQMPIRKIRSLGGKL
  GASVIEILGIEYMGELTQFTESQLQSHFGEKNGSWLYAMCRGIEHDPVKPRQLPKTIGCSKNFPGKTAL
  ATREQVQWWLLQLAQELEERLTKDRNDNDRVATQLVVSIRVQGDKRLSSLRRCCALTRYDAHKMSHDAF
  TVIKNCNTSGIQTEWSPPLTMLFLCATKFSASAPSSSTDITSFLSSDPSSLPKVPVTSSEAKTQGSGPA
  VTATKKATTSLESFFQKAAERQKVKEASLSSLTAPTQAPMSNSPSKPSLPFQTSQSTGTEPFFKQKSLL
  LKQKQLNNSSVSSPQQNPWSNCKALPNSLPTEYPGCVPVCEGVSKLEESSKATPAEMDLAHNSQSMHAS
  SASKSVLEVTQKATPNPSLLAAEDQVPCEKCGSLVPVWDMPEHMDYHFALELQKSFLQPHSSNPQVVSA
  VSHQGKRNPKSPLACTNKRPRPEGMQTLESFFKPLTH
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001291969
• ORF Size: 1767 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001291969.2
• RefSeq Size: 8053 bp
• RefSeq ORF: 1770 bp
• Locus ID: 5429
• Cytogenetics: 6p21.1
• Protein Families: Druggable Genome
• MW: 65.3 kDa
• Gene Summary: 'This gene encodes a member of the Y family of specialized DNA polymerases. It copies undamaged DNA with a lower fidelity than other DNA-directed polymerases. However, it accurately replicates UV-damaged DNA; when thymine dimers are present, this polymerase inserts the complementary nucleotides in the newly synthesized DNA, thereby bypassing the lesion and suppressing the mutagenic effect of UV-induced DNA damage. This polymerase is thought to be involved in hypermutation during immunoglobulin class switch recombination. Mutations in this gene result in XPV, a variant type of xeroderma pigmentosum. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2014]'