MCCC1 (NM_001293273) Human Tagged ORF Clone

CAT#: RG239070

MCCC1 (GFP-tagged) - Human methylcrotonoyl-CoA carboxylase 1 (alpha) (MCCC1), transcript variant 2

Specifications

Product Data

• Tag: TurboGFP
• Symbol: MCCC1
• Synonyms: MCC-B; MCCA
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG239070 representing NM_001293273.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGGTGTGGAGGCAAAGAACCATGAAGTACACAACAGCCACAGGCAGATGAAGCATATTCCATCGGCC
  CCGCTCCCTCCCAGCAGAGCTACCTATCTATGGAGAAAATCATTCAAGTGGCCAAGACCTCTGCTGCAC
  AGCACATCCAAATCCATAATGGCTGCTGCTGGAGTACCTGTTGTGGAGGGTTATCATGGTGAGGACCAA
  TCAGACCAGTGCCTGAAGGAACACGCCAGGAGAATTGGCTATCCTGTCATGATTAAAGCCGTCCGGGGT
  GGAGGAGGAAAAGGAATGAGGATTGTTAGATCAGAACAAGAATTTCAAGAACAGTTAGAGTCAGCACGG
  AGAGAAGCTAAGAAGTCTTTCAATGATGATGCTATGCTGATCGAGAAGTTTGTAGACACACCGAGGCAT
  GTAGAAGTCCAGGTGTTTGGTGATCACCATGGCAATGCTGTGTACTTGTTTGAAAGAGACTGTAGTGTG
  CAGAGGCGACATCAGAAGATCATTGAGGAGGCCCCAGCGCCTGGTATTAAATCTGAAGTAAGAAAAAAG
  CTGGGAGAAGCTGCAGTCAGAGCTGCTAAAGCTGTAAATTATGTTGGAGCAGGGACTGTGGAGTTTATT
  ATGGACTCAAAACATAATTTCTGTTTCATGGAGATGAATACAAGGCTGCAAGTGGAACATCCTGTTACT
  GAGATGATCACAGGAACTGACTTGGTGGAGTGGCAGCTTAGAATTGCAGCAGGAGAGAAGATTCCTTTG
  AGCCAGGAAGAAATAACTCTGCAGGGCCATGCCTTCGAAGCTAGAATATATGCAGAAGATCCTAGCAAT
  AACTTCATGCCTGTGGCAGGCCCATTAGTGCACCTCTCTACTCCTCGAGCAGACCCTTCCACCAGGATT
  GAAACTGGAGTACGGCAAGGAGACGAAGTTTCCGTGCATTATGACCCCATGATTGCGAAGCTGGTCGTG
  TGGGCAGCAGATCGCCAGGCGGCATTGACAAAACTGAGGTACAGCCTTCGTCAGTACAATATTGTTGGA
  CTGCACACCAACATTGACTTCTTACTCAACCTGTCTGGCCACCCAGAGTTTGAAGCTGGGAACGTGCAC
  ACTGATTTCATCCCTCAACACCACAAACAGTTGTTGCTCAGTCGGAAGGCTGCAGCCAAAGAGTCTTTA
  TGCCAGGCAGCCCTGGGTCTCATCCTCAAGGAGAAAGCCATGACCGACACTTTCACTCTTCAGGCACAT
  GATCAATTCTCTCCATTTTCGTCTAGCAGTGGAAGAAGACTGAATATCTCGTATACCAGAAACATGACT
  CTTAAAGATGGTAAAAACAATGTAGCCATAGCTGTAACGTATAACCATGATGGGTCTTATAGCATGCAG
  ATTGAAGATAAAACTTTCCAAGTCCTTGGTAATCTTTACAGCGAGGGAGACTGCACTTACCTGAAATGT
  TCTGTTAATGGAGTTGCTAGTAAAGCGAAGCTGATTATCCTGGAAAACACTATTTACCTATTTTCCAAG
  GAAGGAAGTATTGAGATTGACATTCCAGTCCCCAAATACTTATCTTCTGTGAGCTCACAAGAAACTCAG
  GGCGGCCCCTTAGCTCCTATGACTGGAACCATTGAAAAGGTGTTTGTCAAAGCTGGAGACAAAGTGAAA
  GCGGGAGATTCCCTCATGGTTATGATCGCCATGAAGATGGAGCATACCATAAAGTCTCCAAAGGATGGC
  ACAGTAAAGAAAGTGTTCTACAGAGAAGGTGCTCAGGCCAACAGACACACTCCTTTAGTCGAGTTTGAG
  GAGGAAGAATCAGACAAAAGGGAATCGGAA
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG239070
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MGVEAKNHEVHNSHRQMKHIPSAPLPPSRATYLWRKSFKWPRPLLHSTSKSIMAAAGVPVVEGYHGEDQ
  SDQCLKEHARRIGYPVMIKAVRGGGGKGMRIVRSEQEFQEQLESARREAKKSFNDDAMLIEKFVDTPRH
  VEVQVFGDHHGNAVYLFERDCSVQRRHQKIIEEAPAPGIKSEVRKKLGEAAVRAAKAVNYVGAGTVEFI
  MDSKHNFCFMEMNTRLQVEHPVTEMITGTDLVEWQLRIAAGEKIPLSQEEITLQGHAFEARIYAEDPSN
  NFMPVAGPLVHLSTPRADPSTRIETGVRQGDEVSVHYDPMIAKLVVWAADRQAALTKLRYSLRQYNIVG
  LHTNIDFLLNLSGHPEFEAGNVHTDFIPQHHKQLLLSRKAAAKESLCQAALGLILKEKAMTDTFTLQAH
  DQFSPFSSSSGRRLNISYTRNMTLKDGKNNVAIAVTYNHDGSYSMQIEDKTFQVLGNLYSEGDCTYLKC
  SVNGVASKAKLIILENTIYLFSKEGSIEIDIPVPKYLSSVSSQETQGGPLAPMTGTIEKVFVKAGDKVK
  AGDSLMVMIAMKMEHTIKSPKDGTVKKVFYREGAQANRHTPLVEFEEEESDKRESE
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001293273
• ORF Size: 1824 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001293273.1, NP_001280202.1
• RefSeq Size: 2302 bp
• RefSeq ORF: 1827 bp
• Locus ID: 56922
• Cytogenetics: 3q27.1
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Valine, leucine and isoleucine degradation
• MW: 68.4 kDa
• Gene Summary: This gene encodes the large subunit of 3-methylcrotonyl-CoA carboxylase. This enzyme functions as a heterodimer and catalyzes the carboxylation of 3-methylcrotonyl-CoA to form 3-methylglutaconyl-CoA. Mutations in this gene are associated with 3-Methylcrotonylglycinuria, an autosomal recessive disorder of leucine catabolism. [provided by RefSeq, Jul 2008]