Calpastatin (CAST) (NM_001284213) Human Tagged ORF Clone

CAT#: RG239168

CAST (GFP-tagged) - Human calpastatin (CAST), transcript variant 14

Specifications

Product Data

• Tag: TurboGFP
• Symbol: CAST
• Synonyms: BS-17; PLACK
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG239168 representing NM_001284213.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGAATCCCACAGAAACCAAGGCTGTAAAAACAGAACCTGAGAAGAAGTCACAGTCAACCAAGCTGTCT
  GTGGTTCATGAGAAAAAATCCCAAGAAGGAAAGCCAAAAGAACACACAGAGCCAAAAAGCCTACCCAAG
  CAGGCATCAGATACAGGAAGTAACGATGCTCACAATAAAAAAGCAGTTTCCAGATCAGCTGAACAGCAG
  CCATCAGAGAAATCAACAGAACCAAAGTCAGGCATGGATGCTGCTTTGGATGACTTAATAGATACTTTA
  GGAGGACCTGAAGAAACTGAAGAAGAAAATACAACGTATACTGGACCAGAAGTTTCAGATCCAATGAGT
  TCCACCTACATAGAGGAATTGGGTAAAAGAGAAGTCACAATTCCTCCAAAATATAGGGAACTATTGGCT
  AAAAAGGAAGGGATCACAGGGCCTCCTGCAGACTCTTCGAAACCCATAGGGCCAGATGATGCTATAGAC
  GCCTTGTCATCTGACTTCACCTGTGGGTCGCCTACAGCTGCTGGAAAGAAAACTGAAAAAGAGGAATCT
  ACAGAAGTTTTAAAAGCTCAGTCAGCAGGGACAGTCAGAAGTGCTGCTCCACCCCAAGAGAAGAAAAGA
  AAGGTGGAGAAGGATACAATGAGTGATCAAGCACTCGAGGCTCTGTCGGCTTCACTGGGCACCCGGCAA
  GCAGAACCTGAGCTCGACCTCCGCTCAATTAAGGAAGTCGATGAGGCAAAAGCTAAAGAAGAAAAACTA
  GAGAAGTGTGGTGAGGATGATGAAACAATCCCATCTGAGTACAGATTAAAACCAGCCACGGATAAAGAT
  GGAAAACCACTATTGCCAGAGCCTGAAGAAAAACCCAAGCCTCGGAGTGAATCAGAACTCATTGATGAA
  CTTTCAGAAGATTTTGACCGGTCTGAATGTAAAGAGAAACCATCTAAGCCAACTGAAAAGACAGAAGAA
  TCTAAGGCCGCTGCTCCAGCTCCTGTGTCGGAGGCTGTGTGTCGGACCTCCATGTGTAGTATACAGTCA
  GCACCCCCTGAGCCGGCTACCTTGAAGGGCACAGTGCCAGATGATGCTGTAGAAGCCTTGGCTGATAGC
  CTGGGGAAAAAGGAAGCAGATCCAGAAGATGGAAAACCTGTGATGGATAAAGTCAAGGAGAAGGCCAAA
  GAAGAAGACCGTGAAAAGCTTGGTGAAAAAGAAGAAACAATTCCTCCTGATTATAGATTAGAAGAGGTC
  AAGGATAAAGATGGAAAGCCACTCCTGCCAAAAGAGTCTAAGGAACAGCTTCCACCCATGAGTGAAGAC
  TTCCTTCTGGATGCTTTGTCTGAGGACTTCTCTGGTCCACAAAATGCTTCATCTCTTAAATTTGAAGAT
  GCTAAACTTGCTGCTGCCATCTCTGAAGTGGTTTCCCAAACCCCAGCTTCAACGACCCAAGCTGGAGCC
  CCACCCCGTGATACCTCGCAGAGTGACAAAGACCTCGATGATGCCTTGGATAAACTCTCTGACAGTCTA
  GGACAAAGGCAGCCTGACCCAGATGAGAACAAACCAATGGAAGATAAAGTAAAGGAAAAAGCTAAAGCT
  GAACATAGAGACAAGCTTGGAGAAAGAGATGACACTATCCCACCTGAATACAGACATCTCCTGGATGAT
  AATGGACAGGACAAACCAGTGAAGCCACCTACAAAGAAATCAGAGGATTCAAAGAAACCTGCAGATGAC
  CAAGACCCCATTGATGCTCTCTCAGGAGATCTGGACAGCTGTCCCTCCACTACAGAAACCTCACAGAAC
  ACAGCAAAGGATAAGTGCAAGAAGGCTGCTTCCAGCTCCAAAGCACCTAAGAATGGAGGTAAAGCGAAG
  GATTCAGCAAAGACAACAGAGGAAACTTCCAAGCCAAAAGATGAC
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG239168
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MNPTETKAVKTEPEKKSQSTKLSVVHEKKSQEGKPKEHTEPKSLPKQASDTGSNDAHNKKAVSRSAEQQ
  PSEKSTEPKSGMDAALDDLIDTLGGPEETEEENTTYTGPEVSDPMSSTYIEELGKREVTIPPKYRELLA
  KKEGITGPPADSSKPIGPDDAIDALSSDFTCGSPTAAGKKTEKEESTEVLKAQSAGTVRSAAPPQEKKR
  KVEKDTMSDQALEALSASLGTRQAEPELDLRSIKEVDEAKAKEEKLEKCGEDDETIPSEYRLKPATDKD
  GKPLLPEPEEKPKPRSESELIDELSEDFDRSECKEKPSKPTEKTEESKAAAPAPVSEAVCRTSMCSIQS
  APPEPATLKGTVPDDAVEALADSLGKKEADPEDGKPVMDKVKEKAKEEDREKLGEKEETIPPDYRLEEV
  KDKDGKPLLPKESKEQLPPMSEDFLLDALSEDFSGPQNASSLKFEDAKLAAAISEVVSQTPASTTQAGA
  PPRDTSQSDKDLDDALDKLSDSLGQRQPDPDENKPMEDKVKEKAKAEHRDKLGERDDTIPPEYRHLLDD
  NGQDKPVKPPTKKSEDSKKPADDQDPIDALSGDLDSCPSTTETSQNTAKDKCKKAASSSKAPKNGGKAK
  DSAKTTEETSKPKDD
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001284213
• ORF Size: 1908 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001284213.3
• RefSeq Size: 4137 bp
• RefSeq ORF: 1911 bp
• Locus ID: 831
• Cytogenetics: 5q15
• MW: 69.5 kDa
• Gene Summary: 'The protein encoded by this gene is an endogenous calpain (calcium-dependent cysteine protease) inhibitor. It consists of an N-terminal domain L and four repetitive calpain-inhibition domains (domains 1-4), and it is involved in the proteolysis of amyloid precursor protein. The calpain/calpastatin system is involved in numerous membrane fusion events, such as neural vesicle exocytosis and platelet and red-cell aggregation. The encoded protein is also thought to affect the expression levels of genes encoding structural or regulatory proteins. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jun 2010]'