EYA4 (NM_001301013) Human Tagged ORF Clone

CAT#: RG239190

EYA4 (GFP-tagged) - Human EYA transcriptional coactivator and phosphatase 4 (EYA4), transcript variant 6

Specifications

Product Data

• Tag: TurboGFP
• Symbol: EYA4
• Synonyms: CMD1J; DFNA10
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG239190 representing NM_001301013.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGAAGACTCCCAGGATTTAAATGAACAATCAGTAAAGAAAACGTGCACAGAATCAGATGTTTCACAA
  TCTCAGAATTCCAGGTCTATGGAAATGCAGGACCTAGCAAGTCCTCATACTCTTGTTGGAGGTGGTGAT
  ACTCCAGGTAGCTCCAAACTGGAAAAATCTAATCTCAGCAGCACATCAGTTACTACAAATGGGACAGGA
  GGGGAAAACATGACTGTTTTAAACACAGCAGACTGGTTGCTGAGTTGCAACACCCCCTCTTCTGCAACA
  ATGTCTCTTCTTGCAGTCAAAACAGAGCCCTTGAACAGCAGTGAAACCACAGCCACGACTGGAGATGGA
  GCGCTTGACACTTTTACTGGGTCAGTAATTACAAGTAGTGGCTACAGCCCCAGATCAGCACATCAGTAT
  TCCCCACAGCTGTATCCTTCCAAGCCCTATCCACACATTCTTTCTACACCAGCAGCTCAAACAATGTCT
  GCCTATGCAGGCCAGACTCAGTATTCGGGGATGCAGCAGCCAGCCGTCTACACAGCCTACTCACAGACA
  GGACAGCCCTACAGCTTGCCCACTTACGATTTGGGTGTGATGTTGCCAGCCATCAAGACAGAGAGTGGA
  CTTTCCCAAACTCAGTCCCCATTACAGAGTGGCTGCCTCAGTTACAGCCCAGGGTTCTCTACCCCACAG
  CCAGGCCAGACACCTTATTCTTACCAAATGCCAGGTTCTAGTTTTGCACCATCATCTACTATTTATGCA
  AATAATTCAGTTTCCAATTCAACGAATTTCAGTGGTTCACAACAGGATTATCCATCCTATACAGCCTTT
  GGCCAAAACCAGTATGCACAGTATTATTCAGCATCAACGTATGGAGCGTATATGACATCGAATAACACA
  GCCGATGGCACACCCTCTTCAACCTCTACTTATCAGTTGCAGGAATCTCTCCCAGGACTGACTAACCAA
  CCAGGTACAGATCTTCACCCAGGAGAGTTCGATACCATGCAGAGTCCCTCCACACCCATCAAAGATCTT
  GATGAGAGAACCTGTAGGAGTTCTGGGTCAAAGTCCAGAGGAAGAGGCCGGAAAAATAATCCCTCCCCG
  CCTCCTGATAGTGACCTGGAGCGTGTGTTTGTCTGGGATTTGGATGAAACCATCATTGTTTTTCACTCA
  CTGCTCACCGGGTCTTATGCACAGAAGTATGGCAAGGATCCCCCCATGGCTGTAACCCTTGGACTCCGC
  ATGGAAGAAATGATTTTTAATCTTGCTGATACTCATTTGTTTTTTAATGATTTAGAGGAGTGTGATCAA
  GTTCATATAGATGATGTTTCCTCTGATGATAATGGGCAGGACTTAAGTACCTACAGTTTTGCAACTGAT
  GGCTTCCATGCAGCTGCAAGTAGTGCAAACCTTTGTTTGCCAACAGGTGTAAGAGGAGGGGTTGACTGG
  ATGAGGAAGTTGGCTTTTCGTTACAGAAGAGTAAAAGAATTATATAACACCTACAAGAACAACGTTGGA
  GGACTCCTTGGCCCTGCCAAGAGGGATGCCTGGCTACAGTTAAGGGCAGAGATTGAAGGTCTGACAGAT
  TCCTGGCTAACAAATGCACTTAAGTCTTTATCAATTATTAGCACTAGGAGTAACTGCATAAATGTCTTG
  GTAACGACAACTCAACTGATCCCAGCACTTGCGAAGGTTCTACTCTATAGTTTAGGAGGTGCTTTCCCC
  ATTGAGAATATTTACAGTGCAACTAAAATAGGAAAAGAAAGTTGCTTTGAACGAATAATGCAAAGGTTT
  GGCAGAAAAGTAGTGTATGTTGTAATTGGGGATGGTGTAGAAGAAGAACAGGCAGCAAAAAAGCACAAC
  ATGCCCTTCTGGAGGATATCCAGTCACTCAGACCTCCTGGCTCTCCACCAAGCACTGGAATTAGAGTAT
  TTG
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG239190
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MEDSQDLNEQSVKKTCTESDVSQSQNSRSMEMQDLASPHTLVGGGDTPGSSKLEKSNLSSTSVTTNGTG
  GENMTVLNTADWLLSCNTPSSATMSLLAVKTEPLNSSETTATTGDGALDTFTGSVITSSGYSPRSAHQY
  SPQLYPSKPYPHILSTPAAQTMSAYAGQTQYSGMQQPAVYTAYSQTGQPYSLPTYDLGVMLPAIKTESG
  LSQTQSPLQSGCLSYSPGFSTPQPGQTPYSYQMPGSSFAPSSTIYANNSVSNSTNFSGSQQDYPSYTAF
  GQNQYAQYYSASTYGAYMTSNNTADGTPSSTSTYQLQESLPGLTNQPGTDLHPGEFDTMQSPSTPIKDL
  DERTCRSSGSKSRGRGRKNNPSPPPDSDLERVFVWDLDETIIVFHSLLTGSYAQKYGKDPPMAVTLGLR
  MEEMIFNLADTHLFFNDLEECDQVHIDDVSSDDNGQDLSTYSFATDGFHAAASSANLCLPTGVRGGVDW
  MRKLAFRYRRVKELYNTYKNNVGGLLGPAKRDAWLQLRAEIEGLTDSWLTNALKSLSIISTRSNCINVL
  VTTTQLIPALAKVLLYSLGGAFPIENIYSATKIGKESCFERIMQRFGRKVVYVVIGDGVEEEQAAKKHN
  MPFWRISSHSDLLALHQALELEYL
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001301013
• ORF Size: 1935 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001301013.1, NP_001287942.1
• RefSeq Size: 5573 bp
• RefSeq ORF: 1938 bp
• Locus ID: 2070
• Cytogenetics: 6q23.2
• Protein Families: Druggable Genome, Phosphatase, Transcription Factors
• MW: 70.6 kDa
• Gene Summary: This gene encodes a member of the eyes absent (EYA) family of proteins. The encoded protein may act as a transcriptional activator through its protein phosphatase activity, and it may be important for eye development, and for continued function of the mature organ of Corti. Mutations in this gene are associated with postlingual, progressive, autosomal dominant hearing loss at the deafness, autosomal dominant non-syndromic sensorineural 10 locus. The encoded protein is also a putative oncogene that mediates DNA repair, apoptosis, and innate immunity following DNA damage, cellular damage, and viral attack. Defects in this gene are also associated with dilated cardiomyopathy 1J. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2014]