FUBP1 (NM_001303433) Human Tagged ORF Clone

CAT#: RG239260

FUBP1 (GFP-tagged) - Human far upstream element (FUSE) binding protein 1 (FUBP1), transcript variant 1

Specifications

Product Data

• Tag: TurboGFP
• Symbol: FUBP1
• Synonyms: FBP; FUBP; hDH V
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG239260 representing NM_001303433.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGCAGACTATTCAACAGTGCCTCCCCCCTCTTCTGGCTCAGCTGGTGGCGGTGGTGGCGGCGGTGGT
  GGTGGAGGAGTTAACGACGCTTTCAAAGATGCACTGCAGAGAGCCCGGCAGATTGCAGCAAAAATTGGA
  GGTGATGCAGGGACATCACTGAATTCAAATGACTATGGTTATGGGGGACAAAAAAGACCTTTAGAAGAT
  GGAGATGGCTCTTGGACAAGTCCGAGCAGTACAACACACTGGGAGGGAATGCCCTCTCCTTTTAAAGAT
  CAACCAGATGCTAAGAAAGTTGCTCCTCAAAATGACTCTTTTGGAACACAGTTACCACCGATGCATCAG
  CAGCAAAGCAGATCTGTAATGACAGAAGAATACAAAGTTCCAGATGGAATGGTTGGATTCATAATTGGC
  AGAGGAGGTGAACAGATCTCACGCATACAACAGGAATCTGGATGCAAAATACAGATAGCTCCTGACAGT
  GGTGGCCTTCCAGAAAGGTCCTGTATGTTAACTGGAACACCTGAATCTGTCCAGTCAGCAAAACGGTTA
  CTGGACCAGATTGTTGAAAAAGGAAGACCAGCTCCTGGCTTCCATCATGGCGATGGACCGGGAAATGCA
  GTTCAAGAAATCATGATTCCAGCTAGCAAGGCAGGATTAGTCATTGGAAAAGGGGGAGAAACTATTAAA
  CAGCTTCAGGAACGGGCTGGAGTTAAAATGGTTATGATTCAAGACGGGCCGCAGAACACTGGTGCTGAC
  AAACCTCTTAGGATTACAGGAGACCCATATAAAGTTCAACAAGCCAAGGAAATGGTGTTAGAGTTAATT
  CGTGATCAAGGCGGTTTCAGAGAAGTTCGGAATGAGTATGGGTCAAGAATAGGAGGAAATGAAGGGATA
  GATGTCCCCATTCCAAGATTTGCTGTTGGCATTGTAATAGGAAGAAATGGAGAGATGATCAAAAAAATA
  CAAAATGATGCTGGTGTTCGCATTCAGTTTAAGCCAGATGATGGGACAACACCCGAAAGGATAGCACAA
  ATAACAGGACCTCCAGACCGATGTCAACATGCTGCAGAAATTATTACAGACCTTCTTCGAAGTGTTCAG
  GCTGGTAATCCTGGTGGACCTGGACCTGGTGGTCGAGGAAGAGGTAGAGGTCAAGGCAACTGGAACATG
  GGACCACCTGGTGGACTACAGGAATTTAATTTTATTGTGCCAACTGGGAAAACTGGATTAATAATAGGA
  AAAGGAGGTGAAACCATAAAAAGCATAAGCCAGCAGTCTGGTGCAAGAATAGAACTTCAGAGAAATCCT
  CCACCAAATGCAGATCCTAATATGAAGTTATTTACAATTCGTGGCACTCCACAACAGATAGACTATGCT
  CGGCAACTCATAGAAGAAAAGATTGGTGGCCCAGTAAATCCTTTAGGGCCACCTGTACCCCATGGGCCC
  CATGGTGTCCCAGGCCCCCATGGACCTCCTGGGCCTCCAGGGCCTGGAACTCCAATGGGACCATACAAC
  CCTGCACCTTATAATCCTGGACCACCAGGCCCGGCTCCTCATGGTCCTCCAGCCCCATATGCTCCCCAG
  GGATGGGGAAATGCATATCCACACTGGCAGCAGCAGGCTCCTCCTGATCCAGCTAAGGCAGGAACGGAT
  CCAAATTCAGCAGCTTGGGCTGCTTATTACGCTCACTATTATCAACAGCAAGCACAGCCACCACCAGCA
  GCCCCTGCAGGTGCACCAACTACAACTCAAACTAATGGACAAGGAGATCAGCAGAATCCAGCCCCAGCT
  GGACAGGTTGATTATACCAAGGCTTGGGAAGAGTACTACAAGAAAATGGGTCAGGCAGTTCCTGCTCCG
  ACTGGGGCTCCTCCAGGTGGTCAGCCAGATTATAGTGCAGCCTGGGCTGAGTATTATAGACAACAAGCA
  GCCTATTATGCCCAGACAAGTCCCCAGGGAATGCCACAGCATCCTCCAGCACCTCAGGGCCAA
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG239260
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MADYSTVPPPSSGSAGGGGGGGGGGGVNDAFKDALQRARQIAAKIGGDAGTSLNSNDYGYGGQKRPLED
  GDGSWTSPSSTTHWEGMPSPFKDQPDAKKVAPQNDSFGTQLPPMHQQQSRSVMTEEYKVPDGMVGFIIG
  RGGEQISRIQQESGCKIQIAPDSGGLPERSCMLTGTPESVQSAKRLLDQIVEKGRPAPGFHHGDGPGNA
  VQEIMIPASKAGLVIGKGGETIKQLQERAGVKMVMIQDGPQNTGADKPLRITGDPYKVQQAKEMVLELI
  RDQGGFREVRNEYGSRIGGNEGIDVPIPRFAVGIVIGRNGEMIKKIQNDAGVRIQFKPDDGTTPERIAQ
  ITGPPDRCQHAAEIITDLLRSVQAGNPGGPGPGGRGRGRGQGNWNMGPPGGLQEFNFIVPTGKTGLIIG
  KGGETIKSISQQSGARIELQRNPPPNADPNMKLFTIRGTPQQIDYARQLIEEKIGGPVNPLGPPVPHGP
  HGVPGPHGPPGPPGPGTPMGPYNPAPYNPGPPGPAPHGPPAPYAPQGWGNAYPHWQQQAPPDPAKAGTD
  PNSAAWAAYYAHYYQQQAQPPPAAPAGAPTTTQTNGQGDQQNPAPAGQVDYTKAWEEYYKKMGQAVPAP
  TGAPPGGQPDYSAAWAEYYRQQAAYYAQTSPQGMPQHPPAPQGQ
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001303433
• ORF Size: 1995 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001303433.1, NP_001290362.1
• RefSeq Size: 2585 bp
• RefSeq ORF: 1998 bp
• Locus ID: 8880
• Cytogenetics: 1p31.1
• Protein Families: Stem cell - Pluripotency, Transcription Factors
• MW: 70.3 kDa
• Gene Summary: The protein encoded by this gene is a single stranded DNA-binding protein that binds to multiple DNA elements, including the far upstream element (FUSE) located upstream of c-myc. Binding to FUSE occurs on the non-coding strand, and is important to the regulation of c-myc in undifferentiated cells. This protein contains three domains, an amphipathic helix N-terminal domain, a DNA-binding central domain, and a C-terminal transactivation domain that contains three tyrosine-rich motifs. The N-terminal domain is thought to repress the activity of the C-terminal domain. This protein is also thought to bind RNA, and contains 3'-5' helicase activity with in vitro activity on both DNA-DNA and RNA-RNA duplexes. Aberrant expression of this gene has been found in malignant tissues, and this gene is important to neural system and lung development. Binding of this protein to viral RNA is thought to play a role in several viral diseases, including hepatitis C and hand, foot and mouth disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]