PRMT7 (NM_001290018) Human Tagged ORF Clone

CAT#: RG239320

PRMT7 (GFP-tagged) - Human protein arginine methyltransferase 7 (PRMT7), transcript variant 3

Specifications

Product Data

• Tag: TurboGFP
• Symbol: PRMT7
• Synonyms: SBIDDS
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG239320 representing NM_001290018.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGAAGATCTTCTGCAGTCGGGCCAATCCGACCACGGGGTCTGTGGAGTGGCTGGAGGAGGATGAACAC
  TATGATTACCACCAGGAGATTGCAAGGTCATCTTATGCAGATATGCTACATGACAAAGACAGAAATGTA
  AAATACTACCAAGGTATCCGGGCTGCCGTGAGCAGGGTGAAGGACAGAGGACAGAAGGCCTTGGTTCTC
  GACATTGGCACTGGCACGGGACTCTTGTCAATGATGGCGGTCACAGCAGGTGCCGACTTCTGCTATGCC
  ATCGAGGTTTTCAAGCCTATGGCTGATGCTGCTGTGAAGATTGTGGAGAAAAATGGCTTTAGTGATAAG
  ATTAAGGTTATCAACAAGCATTCCACCGAGGTGACTGTAGGTCCAGAGGGTGACATGCCATGCCGTGCC
  AACATCCTGGTCACAGAGTTGTTTGACACAGAGCTGATCGGGGAGGGGGCGCTGCCCTCCTATGAGCAC
  GCACACAGGCATCTCGTGGAGGAAAATTGTGAGGCCGTGCCCCACAGAGCCACCGTCTATGCACAGCTG
  GTGGAGTCCGGGAGGATGTGGTCGTGGAACAAGCTATTTCCCATCCACGTGCAGACCAGCCTCGGAGAG
  CAGGTCATCGTCCCTCCCGTTGACGTGGAGAGCTGCCCTGGCGCACCCTCTGTCTGTGACATTCAGCTG
  AACCAGGTGTCACCAGCCGACTTTACAGTCCTCAGCGATGTGCTGCCCATGTTCAGCATAGACTTCAGC
  AAGCAAGTCAGTAGCTCAGCAGCCTGCCATAGCAGGCGGTTTGAACCTCTGACATCTGGCCGAGCTCAG
  GTGGTTCTCTCGTGGTGGGACATTGAAATGGACCCTGAGGGGAAGATCAAGTGCACCATGGCCCCCTTC
  TGGGCACACTCAGACCCAGAGGAGATGCAGTGGCGGGACCACTGGATGCAGTGTGTGTACTTCCTGCCA
  CAAGAGGAGCCTGTGGTGCAGGGCTCAGCGCTCTATCTGGTAGCCCACCACGATGACTACTGCGTATGG
  TACAGCCTGCAGAGGACCAGCCCTGAAAAGAATGAGAGAGTCCGCCAGATGCGCCCCGTGTGTGACTGC
  CAGGCTCACCTGCTCTGGAACCGGCCTCGGTTTGGAGAGATCAATGACCAGGACAGAACTGATCGATAC
  GTCCAGGCTCTGAGGACCGTGCTGAAGCCAGACAGCGTGTGCCTGTGTGTCAGCGATGGCAGCCTGCTC
  TCCGTGCTGGCCCATCACCTGGGGGTGGAGCAGGTGTTTACAGTCGAGAGTTCAGCAGCTTCTCACAAA
  CTGTTGAGAAAAATCTTCAAGGCTAACCACTTGGAAGATAAAATTAACATCATAGAGAAACGGCCGGAA
  TTATTAACAAATGAGGACCTACAGGGCAGAAAGGTCTCTCTCCTCCTGGGCGAGCCGTTCTTCACTACC
  AGCCTGCTGCCGTGGCACAACCTCTACTTCTGGTACGTGCGGACCGCTGTGGACCAGCACCTGGGGCCA
  GGTGCCATGGTGATGCCCCAGGCAGCCTCGCTGCACGCTGTGGTTGTGGAGTTCAGGGACCTGTGGCGG
  ATCCGGAGCCCCTGTGGTGACTGCGAAGGCTTCGACGTGCACATCATGGACGACATGATTAAGCGTGCC
  CTGGACTTCAGGGAGAGCAGGGAAGCTGAGCCCCACCCGCTGTGGGAGTACCCATGCCGCAGCCTCTCC
  GAGCCCTGGCAGATCCTGACCTTTGACTTCCAGCAGCCGGTGCCCCTGCAGCCCCTGTGTGCCGAGGGC
  ACCGTGGAGCTCAGAAGGCCCGGGCAGAGCCACGCAGCGGTGCTATGGATGGAGTACCACCTGACCCCG
  GAGTGCACGCTCAGCACTGGCCTCCTGGAGCCTGCAGACCCCGAGGGGGGCTGCTGCTGGAACCCCCAC
  TGCAAGCAGGCCGTCTACTTCTTCAGCCCTGCCCCAGATCCCAGAGCACTGCTGGGTGGCCCACGGACT
  GTCAGCTATGCAGTGGAGTTTCACCCCGACACAGGCGACATCATCATGGAGTTCAGGCATGCAGATACC
  CCAGAC
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG239320
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MKIFCSRANPTTGSVEWLEEDEHYDYHQEIARSSYADMLHDKDRNVKYYQGIRAAVSRVKDRGQKALVL
  DIGTGTGLLSMMAVTAGADFCYAIEVFKPMADAAVKIVEKNGFSDKIKVINKHSTEVTVGPEGDMPCRA
  NILVTELFDTELIGEGALPSYEHAHRHLVEENCEAVPHRATVYAQLVESGRMWSWNKLFPIHVQTSLGE
  QVIVPPVDVESCPGAPSVCDIQLNQVSPADFTVLSDVLPMFSIDFSKQVSSSAACHSRRFEPLTSGRAQ
  VVLSWWDIEMDPEGKIKCTMAPFWAHSDPEEMQWRDHWMQCVYFLPQEEPVVQGSALYLVAHHDDYCVW
  YSLQRTSPEKNERVRQMRPVCDCQAHLLWNRPRFGEINDQDRTDRYVQALRTVLKPDSVCLCVSDGSLL
  SVLAHHLGVEQVFTVESSAASHKLLRKIFKANHLEDKINIIEKRPELLTNEDLQGRKVSLLLGEPFFTT
  SLLPWHNLYFWYVRTAVDQHLGPGAMVMPQAASLHAVVVEFRDLWRIRSPCGDCEGFDVHIMDDMIKRA
  LDFRESREAEPHPLWEYPCRSLSEPWQILTFDFQQPVPLQPLCAEGTVELRRPGQSHAAVLWMEYHLTP
  ECTLSTGLLEPADPEGGCCWNPHCKQAVYFFSPAPDPRALLGGPRTVSYAVEFHPDTGDIIMEFRHADT
  PD
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001290018
• ORF Size: 2076 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001290018.1, NP_001276947.1
• RefSeq Size: 2984 bp
• RefSeq ORF: 2079 bp
• Locus ID: 54496
• Cytogenetics: 16q22.1
• Protein Families: Druggable Genome
• MW: 78.9 kDa
• Gene Summary: This gene encodes a member of the protein arginine N-methyltransferase family of proteins. The encoded enzyme transfers single methyl groups to arginine residues to generate monomethylarginines on histone proteins as well as other protein substrates. This enzyme plays a role in a wide range of biological processes, including neuronal differentiation, male germ line imprinting, small nuclear ribonucleoprotein biogenesis, and regulation of the Wnt signaling pathway. Mutations in this gene underlie multiple related syndromes in human patients characterized by intellectual disability, short stature and other features. The encoded protein may promote breast cancer cell invasion and metastasis in human patients. [provided by RefSeq, May 2017]