STT3A (NM_001278503) Human Tagged ORF Clone

CAT#: RG239365

STT3A (GFP-tagged) - Human STT3A, subunit of the oligosaccharyltransferase complex (catalytic) (STT3A), transcript variant 1

Specifications

Product Data

• Tag: TurboGFP
• Symbol: STT3A
• Synonyms: ITM1; STT3-A; TMC
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG239365 representing NM_001278503.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGACTAAGTTTGGATTTTTGCGATTGTCCTATGAGAAGCAGGACACACTTTTGAAGCTTCTCATTCTG
  TCAATGGCTGCTGTATTATCCTTCTCCACTCGTCTGTTTGCTGTCCTGAGATTTGAAAGTGTTATCCAT
  GAGTTTGATCCGTACTTTAATTATCGGACTACCAGGTTCCTGGCTGAGGAGGGGTTTTATAAATTCCAT
  AACTGGTTTGATGACCGAGCCTGGTACCCTTTGGGACGAATCATTGGAGGAACAATTTACCCAGGTTTA
  ATGATCACCTCTGCTGCAATCTACCATGTACTCCATTTTTTCCACATCACCATCGACATTCGGAATGTC
  TGTGTGTTCCTGGCCCCTCTCTTCTCCTCCTTCACCACCATCGTCACGTACCACCTTACCAAAGAGCTC
  AAGGATGCAGGGGCTGGGCTTCTTGCTGCTGCCATGATTGCTGTAGTTCCTGGATATATCTCCCGATCT
  GTGGCTGGCTCCTATGATAATGAAGGGATTGCCATCTTTTGCATGCTACTCACCTACTACATGTGGATC
  AAGGCAGTAAAGACTGGTTCCATCTGTTGGGCAGCTAAGTGTGCCCTTGCTTATTTCTACATGGTCTCG
  TCATGGGGAGGTTATGTGTTCCTGATCAACTTAATTCCTCTCCACGTCCTCGTGCTGATGCTCACAGGC
  CGTTTCTCTCACCGGATCTATGTGGCCTACTGTACTGTTTACTGCCTGGGCACTATACTTTCTATGCAG
  ATCTCCTTTGTGGGTTTCCAGCCTGTCCTTTCATCAGAGCACATGGCAGCCTTTGGGGTCTTTGGTCTC
  TGCCAGATCCATGCCTTTGTGGATTACCTGCGCAGCAAGTTGAATCCACAACAATTTGAAGTTCTTTTC
  CGGAGCGTCATCTCTCTGGTAGGCTTTGTCCTTCTCACCGTGGGAGCTCTCCTCATGCTGACAGGAAAA
  ATATCTCCCTGGACGGGGCGTTTCTACTCGCTGCTGGATCCCTCTTATGCTAAGAACAACATCCCCATC
  ATTGCTTCTGTGTCTGAGCATCAGCCCACAACCTGGTCCTCATACTATTTTGACCTGCAGCTCCTCGTC
  TTCATGTTTCCAGTTGGCCTCTATTACTGCTTTAGCAACCTGTCTGATGCCCGGATTTTTATCATCATG
  TATGGTGTGACCAGCATGTACTTTTCAGCTGTAATGGTGCGTCTAATGCTAGTGTTGGCACCTGTTATG
  TGCATTCTCTCTGGCATTGGAGTCTCCCAGGTGCTGTCCACATACATGAAGAATCTGGACATAAGTCGT
  CCAGACAAGAAGAGCAAGAAGCAACAGGATTCCACCTACCCTATTAAGAATGAAGTGGCAAGTGGGATG
  ATACTGGTCATGGCTTTCTTTCTCATCACCTACACCTTTCATTCAACCTGGGTGACCAGTGAGGCCTAC
  TCTTCTCCGTCCATTGTACTATCTGCCCGTGGTGGGGATGGCAGTAGGATCATATTTGATGACTTCCGA
  GAAGCATATTATTGGCTTCGTCATAATACTCCAGAGGATGCGAAGGTCATGTCCTGGTGGGATTATGGC
  TATCAGATTACAGCTATGGCAAACCGAACAATTTTAGTGGACAATAACACATGGAATAATACCCATATT
  TCTCGAGTAGGGCAGGCAATGGCGTCCACAGAGGAAAAAGCCTATGAGATCATGAGGGAGCTCGATGTC
  AGCTATGTGCTGGTCATTTTTGGAGGCCTCACTGGGTATTCCTCTGATGATATCAACAAGTTTCTTTGG
  ATGGTCCGGATTGGAGGGAGCACAGATACAGGCAAACATATCAAGGAGAATGACTATTATACTCCAACT
  GGGGAGTTCCGTGTGGACCGTGAAGGTTCTCCAGTGCTGCTCAACTGCCTCATGTACAAGATGTGTTAC
  TATCGCTTTGGACAGGTTTACACAGAAGCCAAGCGTCCTCCAGGCTTTGACCGTGTCCGAAATGCTGAG
  ATTGGGAATAAAGACTTTGAGCTTGATGTCCTGGAGGAAGCATATACCACAGAACATTGGCTGGTCAGG
  ATATACAAGGTAAAGGACCTGGATAATCGAGGCTTGTCAAGGACA
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG239365
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MTKFGFLRLSYEKQDTLLKLLILSMAAVLSFSTRLFAVLRFESVIHEFDPYFNYRTTRFLAEEGFYKFH
  NWFDDRAWYPLGRIIGGTIYPGLMITSAAIYHVLHFFHITIDIRNVCVFLAPLFSSFTTIVTYHLTKEL
  KDAGAGLLAAAMIAVVPGYISRSVAGSYDNEGIAIFCMLLTYYMWIKAVKTGSICWAAKCALAYFYMVS
  SWGGYVFLINLIPLHVLVLMLTGRFSHRIYVAYCTVYCLGTILSMQISFVGFQPVLSSEHMAAFGVFGL
  CQIHAFVDYLRSKLNPQQFEVLFRSVISLVGFVLLTVGALLMLTGKISPWTGRFYSLLDPSYAKNNIPI
  IASVSEHQPTTWSSYYFDLQLLVFMFPVGLYYCFSNLSDARIFIIMYGVTSMYFSAVMVRLMLVLAPVM
  CILSGIGVSQVLSTYMKNLDISRPDKKSKKQQDSTYPIKNEVASGMILVMAFFLITYTFHSTWVTSEAY
  SSPSIVLSARGGDGSRIIFDDFREAYYWLRHNTPEDAKVMSWWDYGYQITAMANRTILVDNNTWNNTHI
  SRVGQAMASTEEKAYEIMRELDVSYVLVIFGGLTGYSSDDINKFLWMVRIGGSTDTGKHIKENDYYTPT
  GEFRVDREGSPVLLNCLMYKMCYYRFGQVYTEAKRPPGFDRVRNAEIGNKDFELDVLEEAYTTEHWLVR
  IYKVKDLDNRGLSRT
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001278503
• ORF Size: 2115 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001278503.2
• RefSeq Size: 4332 bp
• RefSeq ORF: 2118 bp
• Locus ID: 3703
• Cytogenetics: 11q24.2
• Protein Families: Transmembrane
• Protein Pathways: Metabolic pathways, N-Glycan biosynthesis
• MW: 80.5 kDa
• Gene Summary: 'The protein encoded by this gene is a catalytic subunit of the N-oligosaccharyltransferase (OST) complex, which functions in the endoplasmic reticulum to transfer glycan chains to asparagine residues of target proteins. A separate complex containing a similar catalytic subunit with an overlapping function also exists. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2015]'