HSPC302 (TBCK) (NM_001290768) Human Tagged ORF Clone

CAT#: RG239411

TBCK (GFP-tagged) - Human TBC1 domain containing kinase (TBCK), transcript variant 5

Specifications

Product Data

• Tag: TurboGFP
• Symbol: TBCK
• Synonyms: HSPC302; IHPRF3; TBCKL
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG239411 representing NM_001290768.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGCCAAGTAAAAAACCATTGCCTTCTGGCCCCAAATCAGATGTATGGTCTCTTGGAATCATTTTATTT
  GAGCTTTGTGTGGGAAGAAAATTATTTCAGAGCTTGGATATTTCTGAAAGACTAAAATTTTTGCTTACT
  TTGGATTGTGTAGATGACACTTTAATAGTTCTGGCTGAAGAGCATGGTTGTTTGGACATTATAAAGGAG
  CTTCCTGAAACTGTGATAGATCTTTTGAATAAGTGCCTTACCTTCCATCCTTCTAAGAGGCCAACCCCA
  GATGAATTAATGAAGGACAAAGTATTCAGTGAGGTATCACCTTTATATACCCCCTTTACCAAACCTGCC
  AGTCTGTTTTCATCTTCTCTGAGATGTGCTGATTTAACTCTGCCTGAGGATATCAGTCAGTTGTGTAAA
  GATATAAATAATGATTACCTGGCAGAAAGATCTATTGAAGAAGTGTATTACCTTTGGTGTTTGGCTGGA
  GGTGACTTGGAGAAAGAGCTTGTCAACAAGGAAATCATTCGATCCAAACCACCTATCTGCACACTCCCC
  AATTTTCTCTTTGAGGATGGTGAAAGCTTTGGACAAGGTCGAGATAGAAGCTCGCTTTTAGATGATACC
  ACTGTGACATTGTCGTTATGCCAGCTAAGAAATAGATTGAAAGATGTTGGTGGAGAAGCATTTTACCCA
  TTACTTGAAGATGACCAGTCTAATTTACCTCATTCAAACAGCAATAATGAGTTGTCTGCAGCTGCCACG
  CTCCCTTTAATCATCAGAGAGAAGGATACAGAGTACCAACTAAATAGAATTATTCTCTTCGACAGGCTG
  CTAAAGGCTTATCCATATAAAAAAAACCAAATCTGGAAAGAAGCAAGAGTTGACATTCCTCCTCTTATG
  AGAGGTTTAACCTGGGCTGCTCTTCTGGGAGTTGAGGGAGCTATTCATGCCAAGTACGATGCAATTGAT
  AAAGACACTCCAATTCCTACAGATAGACAAATTGAAGTGGATATTCCTCGCTGTCATCAGTACGATGAA
  CTGTTATCATCACCAGAAGGTCATGCAAAATTTAGGCGTGTATTAAAAGCCTGGGTAGTGTCTCATCCT
  GATCTTGTGTATTGGCAAGGTCTTGACTCACTTTGTGCTCCATTCCTATATCTAAACTTCAATAATGAA
  GCCTTGGCTTATGCATGTATGTCTGCTTTTATTCCCAAATACCTGTATAACTTCTTCTTAAAAGACAAC
  TCACATGTAATACAAGAGTATCTGACTGTCTTCTCTCAGATGATTGCATTTCATGATCCAGAGCTGAGT
  AATCATCTCAATGAGATTGGTTTCATTCCAGATCTCTATGCCATCCCTTGGTTTCTTACCATGTTTACT
  CATGTATTTCCACTACACAAAATTTTCCACCTCTGGGATACCTTACTACTTGGGAATTCCTCTTTCCCA
  TTCTGTATTGGAGTAGCAATTCTTCAGCAGCTGCGGGACCGGCTTTTGGCTAATGGCTTTAATGAGTGT
  ATTCTTCTCTTCTCCGATTTACCAGAAATTGACATTGAACGCTGTGTGAGAGAATCTATCAACCTGTTT
  TGTTGGACTCCTAAAAGTGCTACTTACAGACAGCATGCTCAACCTCCAAAGCCATCTTCTGACAGCAGT
  GGAGGCAGAAGTTCGGCACCTTATTTCTCTGCTGAGTGTCCAGATCCTCCAAAGACAGATCTGTCAAGA
  GAATCCATCCCATTAAATGACCTGAAGTCAGAAGTATCACCACGGATTTCAGCAGAGGACCTGATTGAC
  TTGTGTGAGCTCACAGTGACAGGCCACTTCAAAACACCCAGCAAGAAAACAAAGTCCAGTAAACCAAAG
  CTCCTGGTGGTTGACATCCGGAATAGTGAAGACTTTATTCGTGGTCACATTTCAGGAAGCATCAACATT
  CCATTCAGTGCTGCCTTCACTGCAGAAGGGGAGCTTACCCAGGGCCCTTACACTGCTATGCTCCAGAAC
  TTCAAAGGGAAGGTCATTGTCATCGTGGGGCATGTGGCAAAACACACAGCTGAGTTTGCAGCTCACCTT
  GTGAAGATGAAATATCCAAGAATCTGTATTCTAGATGGTGGCATTAATAAAATAAAGCCAACAGGCCTC
  CTCACCATCCCATCTCCTCAAATA
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG239411
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MPSKKPLPSGPKSDVWSLGIILFELCVGRKLFQSLDISERLKFLLTLDCVDDTLIVLAEEHGCLDIIKE
  LPETVIDLLNKCLTFHPSKRPTPDELMKDKVFSEVSPLYTPFTKPASLFSSSLRCADLTLPEDISQLCK
  DINNDYLAERSIEEVYYLWCLAGGDLEKELVNKEIIRSKPPICTLPNFLFEDGESFGQGRDRSSLLDDT
  TVTLSLCQLRNRLKDVGGEAFYPLLEDDQSNLPHSNSNNELSAAATLPLIIREKDTEYQLNRIILFDRL
  LKAYPYKKNQIWKEARVDIPPLMRGLTWAALLGVEGAIHAKYDAIDKDTPIPTDRQIEVDIPRCHQYDE
  LLSSPEGHAKFRRVLKAWVVSHPDLVYWQGLDSLCAPFLYLNFNNEALAYACMSAFIPKYLYNFFLKDN
  SHVIQEYLTVFSQMIAFHDPELSNHLNEIGFIPDLYAIPWFLTMFTHVFPLHKIFHLWDTLLLGNSSFP
  FCIGVAILQQLRDRLLANGFNECILLFSDLPEIDIERCVRESINLFCWTPKSATYRQHAQPPKPSSDSS
  GGRSSAPYFSAECPDPPKTDLSRESIPLNDLKSEVSPRISAEDLIDLCELTVTGHFKTPSKKTKSSKPK
  LLVVDIRNSEDFIRGHISGSINIPFSAAFTAEGELTQGPYTAMLQNFKGKVIVIVGHVAKHTAEFAAHL
  VKMKYPRICILDGGINKIKPTGLLTIPSPQI
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001290768
• ORF Size: 2163 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001290768.1, NP_001277697.1
• RefSeq Size: 5410 bp
• RefSeq ORF: 2166 bp
• Locus ID: 93627
• Cytogenetics: 4q24
• Protein Families: Druggable Genome, Protein Kinase
• MW: 81.8 kDa
• Gene Summary: This gene encodes a protein that contains a protein kinase domain, a Rhodanase-like domain and the Tre-2/Bub2/Cdc16 (TBC) domain. The encoded protein is thought to play a role in actin organization, cell growth and cell proliferation by regulating the mammalian target of the rapamycin (mTOR) signaling pathway. This protein may also be involved in the transcriptional regulation of the components of the mTOR complex. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]