TLE 1 (TLE1) (NM_001303103) Human Tagged ORF Clone

CAT#: RG239546

TLE1 (GFP-tagged) - Human transducin-like enhancer of split 1 (E(sp1) homolog, Drosophila) (TLE1), transcript variant 1

Specifications

Product Data

• Tag: TurboGFP
• Symbol: TLE1
• Synonyms: ESG; ESG1; GRG1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG239546 representing NM_001303103.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGTTCCCGCAGAGCCGGCACCCGACGCCGCACCAGGCTGCAGGCCAGCCCTTCAAGTTCACTATCCCG
  GAGTCCCTGGACCGGATTAAAGAGGAATTCCAGTTCCTGCAGGCGCAGTATCACAGCCTTAAATTGGAA
  TGTGAGAAACTGGCAAGTGAAAAGACAGAAATGCAGAGGCACTATGTGATGTATTATGAAATGTCATAT
  GGATTAAACATTGAAATGCACAAACAGACTGAAATCGCCAAGAGATTGAATACGATTTGTGCACAAGTC
  ATCCCATTTCTGTCTCAGGAACATCAACAACAGGTGGCCCAGGCTGTTGAACGTGCCAAACAGGTGACC
  ATGGCAGAATTGAATGCCATCATCGGGGTACGTGGCCTACCAGGTCTACCTCCTACACAGCAGCAGTTG
  CAAGCTCAGCATCTTTCTCATGGCCACGGACCCCCAGTTCCCCTTACGCCTCACCCTTCGGGACTTCAG
  CCTCCTGGAATCCCGCCCCTCGGGGGCAGTGCCGGCCTTCTTGCGCTGTCTAGTGCTCTGAGTGGGCAG
  TCTCACTTGGCAATAAAAGATGACAAGAAGCACCACGATGCAGAGCACCACAGAGACAGAGAGCCGGGC
  ACAAGTAATTCCCTCCTGGTCCCAGACAGTCTAAGAGGCACAGATAAACGCAGAAATGGACCTGAATTT
  TCCAATGACATCAAGAAAAGGAAGGTGGATGATAAGGACTCCAGCCACTATGACAGTGATGGTGACAAA
  AGCGATGACAACTTAGTTGTGGATGTGTCTAATGAGGACCCTTCTTCTCCGCGAGCAAGCCCTGCCCAC
  TCGCCCCGGGAAAATGGAATCGACAAAAATCGCCTGCTAAAGAAGGATGCTTCTAGCAGTCCAGCTTCC
  ACGGCCTCCTCGGCAAGTTCCACTTCTTTGAAATCCAAAGAAATGAGCTTGCATGAAAAAGCCAGCACG
  CCTGTTCTGAAATCCAGCACACCAACGCCTCGGAGCGACATGCCAACGCCGGGCACCAGCGCCACTCCA
  GGCCTCCGTCCAGGTCTCGGCAAGCCTCCAGCCATAGACCCCCTCGTTAACCAAGCGGCAGCTGGCTTG
  AGGACACCCCTGGCAGTGCCCGGCCCATATCCTGCTCCTTTTGGGATGGTCCCCCACGCTGGCATGAAC
  GGCGAGCTGACCAGCCCAGGCGCTGCCTACGCCAGTTTACACAACATGTCGCCCCAGATGAGCGCCGCA
  GCCGCCGCGGCCGCCGTGGTGGCCTACGGGCGCTCCCCCATGGTGGGGTTTGATCCTCCCCCTCACATG
  AGAGTACCTACCATTCCTCCAAACCTGGCAGGAATCCCTGGGGGGAAACCTGCATACTCCTTCCACGTT
  ACTGCAGACGGTCAGATGCAGCCTGTCCCTTTTCCCCCCGACGCCCTCATCGGACCCGGAATCCCCCGG
  CATGCTCGCCAGATCAACACCCTCAACCACGGGGAGGTGGTGTGCGCTGTGACCATCAGCAACCCCACG
  AGACACGTGTACACAGGCGGGAAGGGCTGCGTCAAGGTCTGGGACATCAGCCACCCTGGCAATAAGAGC
  CCTGTCTCCCAGCTCGACTGTCTGAACAGAGACAATTATATCCGTTCCTGTAAATTGCTACCCGATGGC
  TGCACTCTCATAGTGGGAGGGGAAGCCAGTACTTTGTCCATTTGGGACCTGGCGGCTCCAACCCCGCGC
  ATCAAGGCGGAGCTGACGTCCTCGGCCCCCGCCTGCTACGCCCTGGCCATCAGCCCCGATTCCAAGGTC
  TGCTTCTCATGCTGCAGCGACGGCAACATCGCTGTGTGGGATCTGCACAACCAGACACTAGTGAGGCAA
  TTCCAGGGCCACACAGACGGAGCCAGCTGTATTGACATTTCTAATGATGGCACCAAGCTCTGGACGGGT
  GGTTTGGACAACACAGTCAGGTCCTGGGACCTGCGCGAGGGGCGGCAGCTGCAGCAGCACGACTTCACC
  TCCCAGATCTTCTCCCTGGGGTACTGCCCCACCGGGGAGTGGCTGGCAGTGGGCATGGAGAGCAGCAAT
  GTGGAGGTGCTGCACGTGAACAAGCCTGACAAGTACCAGCTGCACCTGCATGAGAGCTGCGTGCTGTCC
  CTGAAATTTGCTTACTGTGGTAAATGGTTTGTGAGTACTGGAAAAGATAACCTCCTCAATGCTTGGCGG
  ACCCCCTATGGAGCCAGCATATTCCAGTCCAAAGAGTCCTCGTCAGTGCTTAGCTGTGACATCTCTGTG
  GATGATAAGTACATAGTCACTGGCTCGGGGGACAAGAAGGCTACAGTCTATGAAGTCATCTAC
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG239546
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MFPQSRHPTPHQAAGQPFKFTIPESLDRIKEEFQFLQAQYHSLKLECEKLASEKTEMQRHYVMYYEMSY
  GLNIEMHKQTEIAKRLNTICAQVIPFLSQEHQQQVAQAVERAKQVTMAELNAIIGVRGLPGLPPTQQQL
  QAQHLSHGHGPPVPLTPHPSGLQPPGIPPLGGSAGLLALSSALSGQSHLAIKDDKKHHDAEHHRDREPG
  TSNSLLVPDSLRGTDKRRNGPEFSNDIKKRKVDDKDSSHYDSDGDKSDDNLVVDVSNEDPSSPRASPAH
  SPRENGIDKNRLLKKDASSSPASTASSASSTSLKSKEMSLHEKASTPVLKSSTPTPRSDMPTPGTSATP
  GLRPGLGKPPAIDPLVNQAAAGLRTPLAVPGPYPAPFGMVPHAGMNGELTSPGAAYASLHNMSPQMSAA
  AAAAAVVAYGRSPMVGFDPPPHMRVPTIPPNLAGIPGGKPAYSFHVTADGQMQPVPFPPDALIGPGIPR
  HARQINTLNHGEVVCAVTISNPTRHVYTGGKGCVKVWDISHPGNKSPVSQLDCLNRDNYIRSCKLLPDG
  CTLIVGGEASTLSIWDLAAPTPRIKAELTSSAPACYALAISPDSKVCFSCCSDGNIAVWDLHNQTLVRQ
  FQGHTDGASCIDISNDGTKLWTGGLDNTVRSWDLREGRQLQQHDFTSQIFSLGYCPTGEWLAVGMESSN
  VEVLHVNKPDKYQLHLHESCVLSLKFAYCGKWFVSTGKDNLLNAWRTPYGASIFQSKESSSVLSCDISV
  DDKYIVTGSGDKKATVYEVIY
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001303103
• ORF Size: 2340 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001303103.1, NP_001290032.1
• RefSeq Size: 4167 bp
• RefSeq ORF: 2343 bp
• Locus ID: 7088
• Cytogenetics: 9q21.32
• Protein Families: Transcription Factors
• MW: 84.6 kDa
• Gene Summary: ''