Hsp105 (HSPH1) (NM_001286505) Human Tagged ORF Clone

CAT#: RG239552

HSPH1 (GFP-tagged) - Human heat shock 105kDa/110kDa protein 1 (HSPH1), transcript variant 4

Specifications

Product Data

• Tag: TurboGFP
• Symbol: HSPH1
• Synonyms: HSP105; HSP105A; HSP105B; NY-CO-25
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG239552 representing NM_001286505.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGCAACGGCGGCCGTTCTCCGGGGACCGGCTGCCCATTGGGTAGAATCTTTCCAGAAGGCTCGAGAA
  GAAGGAAGCGGAAGTGGCACGTGGAGGGGCCGGTGGAGGCGCCGGTCAGTCATATCATTTGGATCAAAA
  AATAGAACAATCGGAGTTGCAGCCAAAAATCAGCAAATCACTCATGCAAACAATACGGTGTCTAACTTC
  AAAAGATTTCATGGCCGAGCATTCAATGACCCCTTCATTCAAAAGGAGAAGGAAAACTTGAGTTACGAT
  TTGGTTCCATTGAAAAATGGTGGAGTTGGAATAAAGGTAATGTACATGGGTGAAGAACATCTATTTAGT
  GTGGAGCAGATAACAGCCATGTTGTTGACTAAGCTGAAGGAAACTGCTGAAAACAGCCTCAAGAAACCA
  GTAACAGATTGTGTTATTTCAGTACTGGGAACAGCTTTTGATCCTTTCTTAGGAGGAAAAAACTTCGAT
  GAAAAGTTAGTGGAACATTTTTGTGCAGAATTTAAAACTAAGTACAAGTTGGATGCAAAATCCAAAATA
  CGAGCACTCCTACGTCTGTATCAGGAATGTGAAAAACTGAAAAAGCTAATGAGCTCTAACAGCACAGAC
  CTTCCACTGAATATCGAATGCTTTATGAATGATAAAGATGTTTCCGGAAAGATGAACAGGTCACAATTT
  GAAGAACTCTGTGCTGAACTTCTGCAAAAGATAGAAGTACCCCTTTATTCACTGTTGGAACAAACTCAT
  CTCAAAGTAGAAGATGTGAGTGCAGTTGAGATTGTTGGAGGCGCTACACGAATTCCAGCTGTGAAGGAA
  AGAATTGCCAAATTCTTTGGAAAAGATATTAGCACAACACTCAATGCAGATGAAGCAGTAGCCAGAGGA
  TGTGCATTACAGTGTGCAATACTTTCCCCGGCATTTAAAGTTAGAGAATTTTCCGTCACAGATGCAGTT
  CCTTTTCCAATATCTCTGATCTGGAACCATGATTCAGAAGATACTGAAGGTGTTCATGAAGTCTTTAGT
  CGAAACCATGCTGCTCCTTTCTCCAAAGTTCTCACCTTTCTGAGAAGGGGGCCTTTTGAGCTAGAAGCT
  TTCTATTCTGATCCCCAAGGAGTTCCATATCCAGAAGCAAAAATAGGCCGCTTTGTAGTTCAGAATGTT
  TCTGCACAGAAAGATGGAGAAAAATCTAGAGTAAAAGTCAAAGTGCGAGTCAACACCCATGGCATTTTC
  ACCATCTCTACGGCATCTATGGTGGAGAAAGTCCCAACTGAGGAGAATGAAATGTCTTCTGAAGCTGAC
  ATGGAGTGTCTGAATCAGAGACCACCAGAAAACCCAGACACTGATAAAAATGTCCAGCAAGACAACAGT
  GAAGCTGGAACACAGCCCCAGGTACAAACTGATGCTCAACAAACCTCACAGTCTCCCCCTTCACCTGAA
  CTTACCTCAGAAGAAAACAAAATCCCAGATGCTGACAAAGCAAATGAAAAAAAAGTTGACCAGCCTCCA
  GAAGCTAAAAAGCCCAAAATAAAGGTGGTGAATGTTGAGCTGCCTATTGAAGCCAACTTGGTCTGGCAG
  TTAGGGAAAGACCTTCTTAACATGTATATTGAGACAGAGGGTAAGATGATAATGCAAGATAAATTGGAA
  AAAGAAAGGAATGATGCTAAAAATGCAGTTGAGGAATATGTGTATGAGTTCAGAGACAAGCTGTGTGGA
  CCATATGAAAAATTTATATGTGAGCAGGATCATCAAAATTTTTTGAGACTCCTCACAGAAACTGAAGAC
  TGGCTGTATGAAGAAGGAGAGGACCAAGCTAAACAAGCATATGTTGACAAGTTGGAAGAATTAATGAAA
  ATTGGCACTCCAGTTAAAGTTCGGTTTCAGGAAGCTGAAGAACGGCCAAAAATGTTTGAAGAACTAGGA
  CAGAGGCTGCAGCATTATGCCAAGATAGCAGCTGACTTCAGAAATAAGGATGAGAAATACAACCATATT
  GATGAGTCTGAAATGAAAAAAGTGGAGAAGTCTGTTAATGAAGTGATGGAATGGATGAATAATGTCATG
  AATGCTCAGGCTAAAAAGAGTCTTGATCAGGATCCAGTTGTACGTGCTCAGGAAATTAAAACAAAAATC
  AAGGAATTGAACAACACATGTGAACCCGTTGTAACACAACCGAAACCAAAAATTGAATCACCCAAACTG
  GAAAGAACTCCAAATGGCCCAAATATTGATAAAAAGGAAGAAGATTTAGAAGACAAAAACAATTTTGGT
  GCTGAACCTCCACATCAGAATGGTGAATGTTACCCTAATGAGAAAAATTCTGTTAATATGGACTTGGAC
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG239552
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MATAAVLRGPAAHWVESFQKAREEGSGSGTWRGRWRRRSVISFGSKNRTIGVAAKNQQITHANNTVSNF
  KRFHGRAFNDPFIQKEKENLSYDLVPLKNGGVGIKVMYMGEEHLFSVEQITAMLLTKLKETAENSLKKP
  VTDCVISVLGTAFDPFLGGKNFDEKLVEHFCAEFKTKYKLDAKSKIRALLRLYQECEKLKKLMSSNSTD
  LPLNIECFMNDKDVSGKMNRSQFEELCAELLQKIEVPLYSLLEQTHLKVEDVSAVEIVGGATRIPAVKE
  RIAKFFGKDISTTLNADEAVARGCALQCAILSPAFKVREFSVTDAVPFPISLIWNHDSEDTEGVHEVFS
  RNHAAPFSKVLTFLRRGPFELEAFYSDPQGVPYPEAKIGRFVVQNVSAQKDGEKSRVKVKVRVNTHGIF
  TISTASMVEKVPTEENEMSSEADMECLNQRPPENPDTDKNVQQDNSEAGTQPQVQTDAQQTSQSPPSPE
  LTSEENKIPDADKANEKKVDQPPEAKKPKIKVVNVELPIEANLVWQLGKDLLNMYIETEGKMIMQDKLE
  KERNDAKNAVEEYVYEFRDKLCGPYEKFICEQDHQNFLRLLTETEDWLYEEGEDQAKQAYVDKLEELMK
  IGTPVKVRFQEAEERPKMFEELGQRLQHYAKIAADFRNKDEKYNHIDESEMKKVEKSVNEVMEWMNNVM
  NAQAKKSLDQDPVVRAQEIKTKIKELNNTCEPVVTQPKPKIESPKLERTPNGPNIDKKEEDLEDKNNFG
  AEPPHQNGECYPNEKNSVNMDLD
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001286505
• ORF Size: 2346 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001286505.1, NP_001273434.1
• RefSeq Size: 4987 bp
• RefSeq ORF: 2349 bp
• Locus ID: 10808
• Cytogenetics: 13q12.3
• Protein Families: Stem cell - Pluripotency
• MW: 89.3 kDa
• Gene Summary: This gene encodes a member of the heat shock protein 70 family of proteins. The encoded protein functions as a nucleotide exchange factor for the molecular chaperone heat shock cognate 71 kDa protein (Hsc70). In addition, this protein plays a distinct but related role as a holdase that inhibits the aggregation of misfolded proteins, including the cystic fibrosis transmembrane conductance regulator (CFTR) protein. Elevated expression of this protein has been observed in numerous human cancers. [provided by RefSeq, Mar 2017]