H6PD (NM_001282587) Human Tagged ORF Clone

CAT#: RG239593

H6PD (GFP-tagged) - Human hexose-6-phosphate dehydrogenase (glucose 1-dehydrogenase) (H6PD), transcript variant 1

Specifications

Product Data

• Tag: TurboGFP
• Symbol: H6PD
• Synonyms: CORTRD1; G6PDH; GDH
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG239593 representing NM_001282587.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGTTAGCAGAGCCTTTTAACTGGCACCCAGGCATGTGGAATATGCTCATAGTGGCGATGTGCTTGGCC
  CTTCTGGGCTGCCTGCAAGCCCAGGAGCTCCAGGGACATGTCTCCATAATCCTGCTGGGAGCAACTGGG
  GACCTGGCTAAGAAGTACTTATGGCAGGGACTGTTCCAGCTGTACCTGGATGAAGCGGGGAGGGGTCAC
  AGTTTTAGCTTCCATGGAGCTGCTCTGACAGCCCCCAAGCAGGGTCAAGAGCTCATGGCCAAGGCCCTG
  GAATCCCTCTCCTGCCCCAAGGACATGGCACCCAGTCACTGTGCAGAGCACAAGGATCAGTTCCTGCAG
  CTGAGCCAGTACCGCCAACTGAAGACGGCCGAGGACTATCAGGCCCTGAACAAGGACATCGAGGCACAG
  CTCCAGCACGCAGGCCTCCGGGAGGCTGGCAGGATCTTCTACTTCTCAGTGCCACCCTTCGCCTATGAA
  GACATTGCCCGCAACATCAACAGTAGCTGCCGGCCAGGCCCGGGCGCCTGGCTGCGGGTTGTCCTTGAG
  AAACCCTTTGGCCATGACCACTTCTCAGCCCAGCAGCTGGCCACAGAACTCGGGACCTTTTTCCAGGAG
  GAGGAGATGTACCGGGTGGACCATTACTTAGGCAAGCAGGCTGTGGCGCAGATCCTGCCTTTCCGAGAC
  CAGAACCGCAAGGCTTTGGACGGCCTCTGGAACCGGCACCATGTGGAGCGGGTGGAGATCATCATGAAA
  GAGACCGTGGATGCTGAAGGCCGCACCAGCTTCTATGAGGAGTACGGTGTCATTCGCGACGTCCTCCAG
  AACCATCTGACGGAGGTCCTCACCCTCGTGGCCATGGAGCTGCCCCACAATGTCAGCAGTGCGGAGGCT
  GTGCTGCGGCACAAGCTTCAGGTCTTCCAGGCGCTGCGGGGCCTGCAGAGGGGCAGTGCCGTCGTGGGC
  CAGTACCAGTCTTACAGTGAGCAGGTGCGCAGAGAGCTGCAGAAGCCAGACAGCTTCCACAGCCTGACG
  CCGACCTTCGCAGCCGTCCTAGTGCACATTGACAACCTTCGCTGGGAGGGCGTGCCTTTCATCCTGATG
  TCTGGCAAAGCCTTGGACGAGAGAGTGGGCTACGCTCGGATCTTGTTCAAGAACCAGGCCTGCTGTGTG
  CAGAGCGAAAAGCACTGGGCCGCGGCGCAGAGCCAGTGCCTGCCCCGGCAGCTCGTCTTCCACATCGGC
  CATGGCGACCTGGGCAGCCCTGCCGTGCTGGTCAGCAGGAACCTGTTCAGGCCCTCCCTGCCCTCCAGC
  TGGAAGGAAATGGAGGGACCACCTGGGCTCCGCCTTTTCGGCAGCCCTCTGTCCGATTACTACGCCTAC
  AGCCCTGTGCGGGAGCGGGACGCCCACTCCGTCCTCTTATCCCATATCTTCCATGGCCGGAAGAATTTC
  TTCATCACCACAGAGAACTTGCTGGCCTCCTGGAACTTCTGGACCCCTCTGCTGGAGAGCCTGGCCCAT
  AAGGCCCCACGCCTCTACCCTGGAGGAGCTGAGAATGGCCGTCTGTTGGACTTTGAGTTCAGTAGCGGC
  CGGTTGTTCTTTTCCCAGCAGCAGCCGGAGCAGCTGGTGCCAGGGCCAGGGCCGGCCCCAATGCCCAGT
  GACTTCCAGGTCCTCAGGGCCAAGTACCGAGAGAGCCCGCTGGTCTCCGCCTGGTCCGAGGAGCTGATC
  TCTAAGCTGGCTAATGACATCGAGGCCACCGCTGTGCGAGCCGTGCGGCGCTTTGGCCAGTTCCACCTG
  GCACTGTCGGGGGGCTCGAGCCCCGTGGCCCTGTTCCAGCAGCTGGCCACGGCGCACTATGGCTTCCCC
  TGGGCCCACACGCACCTGTGGCTGGTTGACGAGCGCTGCGTCCCACTCTCAGACCCGGAGTCCAACTTC
  CAGGGCCTGCAGGCCCACCTGCTGCAGCACGTCCGGATCCCCTACTACAACATCCACCCCATGCCTGTG
  CACCTGCAGCAGCGGCTCTGCGCCGAGGAGGACCAGGGCGCCCAGATCTATGCCAGGGAGATCTCAGCC
  CTGGTGGCCAACAGCAGCTTCGACCTGGTGCTGCTGGGCATGGGTGCCGACGGGCACACAGCCTCCCTC
  TTCCCACAGTCACCCACTGGCCTGGATGGCGAGCAGCTGGTCGTGCTGACCACGAGCCCCTCCCAGCCA
  CACCGCCGCATGAGCCTTAGCCTGCCTCTCATCAACCGCGCCAAGAAGGTGGCAGTCCTGGTCATGGGC
  AGGATGAAGCGTGAGATCACCACGCTGGTGAGCCGGGTGGGCCATGAGCCCAAGAAGTGGCCCATCTCG
  GGTGTCCTGCCGCACTCCGGCCAGCTGGTGTGGTACATGGACTACGACGCCTTCCTGGGA
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG239593
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MLAEPFNWHPGMWNMLIVAMCLALLGCLQAQELQGHVSIILLGATGDLAKKYLWQGLFQLYLDEAGRGH
  SFSFHGAALTAPKQGQELMAKALESLSCPKDMAPSHCAEHKDQFLQLSQYRQLKTAEDYQALNKDIEAQ
  LQHAGLREAGRIFYFSVPPFAYEDIARNINSSCRPGPGAWLRVVLEKPFGHDHFSAQQLATELGTFFQE
  EEMYRVDHYLGKQAVAQILPFRDQNRKALDGLWNRHHVERVEIIMKETVDAEGRTSFYEEYGVIRDVLQ
  NHLTEVLTLVAMELPHNVSSAEAVLRHKLQVFQALRGLQRGSAVVGQYQSYSEQVRRELQKPDSFHSLT
  PTFAAVLVHIDNLRWEGVPFILMSGKALDERVGYARILFKNQACCVQSEKHWAAAQSQCLPRQLVFHIG
  HGDLGSPAVLVSRNLFRPSLPSSWKEMEGPPGLRLFGSPLSDYYAYSPVRERDAHSVLLSHIFHGRKNF
  FITTENLLASWNFWTPLLESLAHKAPRLYPGGAENGRLLDFEFSSGRLFFSQQQPEQLVPGPGPAPMPS
  DFQVLRAKYRESPLVSAWSEELISKLANDIEATAVRAVRRFGQFHLALSGGSSPVALFQQLATAHYGFP
  WAHTHLWLVDERCVPLSDPESNFQGLQAHLLQHVRIPYYNIHPMPVHLQQRLCAEEDQGAQIYAREISA
  LVANSSFDLVLLGMGADGHTASLFPQSPTGLDGEQLVVLTTSPSQPHRRMSLSLPLINRAKKVAVLVMG
  RMKREITTLVSRVGHEPKKWPISGVLPHSGQLVWYMDYDAFLG
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001282587
• ORF Size: 2406 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001282587.2
• RefSeq Size: 9043 bp
• RefSeq ORF: 2409 bp
• Locus ID: 9563
• Cytogenetics: 1p36.22
• Protein Pathways: Metabolic pathways, Pentose phosphate pathway
• MW: 90.6 kDa
• Gene Summary: There are 2 forms of glucose-6-phosphate dehydrogenase. G form is X-linked and H form, encoded by this gene, is autosomally linked. This H form shows activity with other hexose-6-phosphates, especially galactose-6-phosphate, whereas the G form is specific for glucose-6-phosphate. Both forms are present in most tissues, but H form is not found in red cells. [provided by RefSeq, Jul 2008]