Hsp105 (HSPH1) (NM_001286503) Human Tagged ORF Clone

CAT#: RG239619

HSPH1 (GFP-tagged) - Human heat shock 105kDa/110kDa protein 1 (HSPH1), transcript variant 2

Specifications

Product Data

• Tag: TurboGFP
• Symbol: HSPH1
• Synonyms: HSP105; HSP105A; HSP105B; NY-CO-25
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG239619 representing NM_001286503.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGTCGGTGGTGGGGTTGGACGTGGGCTCGCAGAGCTGCTACATCGCGGTAGCCCGGGCCGGGGGCATC
  GAGACCATCGCCAATGAGTTCAGCGACCGGTGCACCCCGTCAGTCATATCATTTGGATCAAAAAATAGA
  ACAATCGGAGTTGCAGCCAAAAATCAGCAAATCACTCATGCAAACAATACGGTGTCTAACTTCAAAAGA
  TTTCATGGCCGAGCATTCAATGACCCCTTCATTCAAAAGGAGAAGGAAAACTTGAGTTACGATTTGGTT
  CCATTGAAAAATGGTGGAGTTGGAATAAAGGTAATGTACATGGGTGAAGAACATCTATTTAGTGTGGAG
  CAGATAACAGCCATGTTGTTGACTAAGCTGAAGGAAACTGCTGAAAACAGCCTCAAGAAACCAGTAACA
  GATTGTGTTATTTCAGTCCCCTCCTTCTTTACAGATGCTGAGAGGCGATCTGTGTTAGATGCTGCACAG
  ATTGTTGGCCTAAACTGTTTAAGACTTATGAATGACATGACAGCTGTTGCTTTGAATTACGGAATTTAT
  AAGCAGGATCTCCCAAGCCTGGATGAGAAACCTCGGATAGTGGTTTTTGTTGATATGGGACATTCAGCT
  TTTCAAGTGTCTGCTTGTGCTTTTAACAAGGGAAAATTGAAGGTACTGGGAACAGCTTTTGATCCTTTC
  TTAGGAGGAAAAAACTTCGATGAAAAGTTAGTGGAACATTTTTGTGCAGAATTTAAAACTAAGTACAAG
  TTGGATGCAAAATCCAAAATACGAGCACTCCTACGTCTGTATCAGGAATGTGAAAAACTGAAAAAGCTA
  ATGAGCTCTAACAGCACAGACCTTCCACTGAATATCGAATGCTTTATGAATGATAAAGATGTTTCCGGA
  AAGATGAACAGGTCACAATTTGAAGAACTCTGTGCTGAACTTCTGCAAAAGATAGAAGTACCCCTTTAT
  TCACTGTTGGAACAAACTCATCTCAAAGTAGAAGATGTGAGTGCAGTTGAGATTGTTGGAGGCGCTACA
  CGAATTCCAGCTGTGAAGGAAAGAATTGCCAAATTCTTTGGAAAAGATATTAGCACAACACTCAATGCA
  GATGAAGCAGTAGCCAGAGGATGTGCATTACAGTGTGCAATACTTTCCCCGGCATTTAAAGTTAGAGAA
  TTTTCCGTCACAGATGCAGTTCCTTTTCCAATATCTCTGATCTGGAACCATGATTCAGAAGATACTGAA
  GGTGTTCATGAAGTCTTTAGTCGAAACCATGCTGCTCCTTTCTCCAAAGTTCTCACCTTTCTGAGAAGG
  GGGCCTTTTGAGCTAGAAGCTTTCTATTCTGATCCCCAAGGAGTTCCATATCCAGAAGCAAAAATAGGC
  CGCTTTGTAGTTCAGAATGTTTCTGCACAGAAAGATGGAGAAAAATCTAGAGTAAAAGTCAAAGTGCGA
  GTCAACACCCATGGCATTTTCACCATCTCTACGGCATCTATGGTGGAGAAAGTCCCAACTGAGGAGAAT
  GAAATGTCTTCTGAAGCTGACATGGAGTGTCTGAATCAGAGACCACCAGAAAACCCAGACACTGATGCA
  AATGAAAAAAAAGTTGACCAGCCTCCAGAAGCTAAAAAGCCCAAAATAAAGGTGGTGAATGTTGAGCTG
  CCTATTGAAGCCAACTTGGTCTGGCAGTTAGGGAAAGACCTTCTTAACATGTATATTGAGACAGAGGGT
  AAGATGATAATGCAAGATAAATTGGAAAAAGAAAGGAATGATGCTAAAAATGCAGTTGAGGAATATGTG
  TATGAGTTCAGAGACAAGCTGTGTGGACCATATGAAAAATTTATATGTGAGCAGGATCATCAAAATTTT
  TTGAGACTCCTCACAGAAACTGAAGACTGGCTGTATGAAGAAGGAGAGGACCAAGCTAAACAAGCATAT
  GTTGACAAGTTGGAAGAATTAATGAAAATTGGCACTCCAGTTAAAGTTCGGTTTCAGGAAGCTGAAGAA
  CGGCCAAAAATGTTTGAAGAACTAGGACAGAGGCTGCAGCATTATGCCAAGATAGCAGCTGACTTCAGA
  AATAAGGATGAGAAATACAACCATATTGATGAGTCTGAAATGAAAAAAGTGGAGAAGTCTGTTAATGAA
  GTGATGGAATGGATGAATAATGTCATGAATGCTCAGGCTAAAAAGAGTCTTGATCAGGATCCAGTTGTA
  CGTGCTCAGGAAATTAAAACAAAAATCAAGGAATTGAACAACACATGTGAACCCGTTGTAACACAACCG
  AAACCAAAAATTGAATCACCCAAACTGGAAAGAACTCCAAATGGCCCAAATATTGATAAAAAGGAAGAA
  GATTTAGAAGACAAAAACAATTTTGGTGCTGAACCTCCACATCAGAATGGTGAATGTTACCCTAATGAG
  AAAAATTCTGTTAATATGGACTTGGAC
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG239619
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MSVVGLDVGSQSCYIAVARAGGIETIANEFSDRCTPSVISFGSKNRTIGVAAKNQQITHANNTVSNFKR
  FHGRAFNDPFIQKEKENLSYDLVPLKNGGVGIKVMYMGEEHLFSVEQITAMLLTKLKETAENSLKKPVT
  DCVISVPSFFTDAERRSVLDAAQIVGLNCLRLMNDMTAVALNYGIYKQDLPSLDEKPRIVVFVDMGHSA
  FQVSACAFNKGKLKVLGTAFDPFLGGKNFDEKLVEHFCAEFKTKYKLDAKSKIRALLRLYQECEKLKKL
  MSSNSTDLPLNIECFMNDKDVSGKMNRSQFEELCAELLQKIEVPLYSLLEQTHLKVEDVSAVEIVGGAT
  RIPAVKERIAKFFGKDISTTLNADEAVARGCALQCAILSPAFKVREFSVTDAVPFPISLIWNHDSEDTE
  GVHEVFSRNHAAPFSKVLTFLRRGPFELEAFYSDPQGVPYPEAKIGRFVVQNVSAQKDGEKSRVKVKVR
  VNTHGIFTISTASMVEKVPTEENEMSSEADMECLNQRPPENPDTDANEKKVDQPPEAKKPKIKVVNVEL
  PIEANLVWQLGKDLLNMYIETEGKMIMQDKLEKERNDAKNAVEEYVYEFRDKLCGPYEKFICEQDHQNF
  LRLLTETEDWLYEEGEDQAKQAYVDKLEELMKIGTPVKVRFQEAEERPKMFEELGQRLQHYAKIAADFR
  NKDEKYNHIDESEMKKVEKSVNEVMEWMNNVMNAQAKKSLDQDPVVRAQEIKTKIKELNNTCEPVVTQP
  KPKIESPKLERTPNGPNIDKKEEDLEDKNNFGAEPPHQNGECYPNEKNSVNMDLD
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001286503
• ORF Size: 2442 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001286503.1, NP_001273432.1
• RefSeq Size: 5228 bp
• RefSeq ORF: 2445 bp
• Locus ID: 10808
• Cytogenetics: 13q12.3
• Protein Families: Stem cell - Pluripotency
• MW: 92.6 kDa
• Gene Summary: This gene encodes a member of the heat shock protein 70 family of proteins. The encoded protein functions as a nucleotide exchange factor for the molecular chaperone heat shock cognate 71 kDa protein (Hsc70). In addition, this protein plays a distinct but related role as a holdase that inhibits the aggregation of misfolded proteins, including the cystic fibrosis transmembrane conductance regulator (CFTR) protein. Elevated expression of this protein has been observed in numerous human cancers. [provided by RefSeq, Mar 2017]