NELL1 (NM_001288713) Human Tagged ORF Clone

CAT#: RG239659

NELL1 (GFP-tagged) - Human NEL-like 1 (chicken) (NELL1), transcript variant 3

Specifications

Product Data

• Tag: TurboGFP
• Symbol: NELL1
• Synonyms: IDH3GL; NRP1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG239659 representing NM_001288713.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGCCGATGGATTTGATTTTAGTTGTGTGGTTCTGTGTGTGCACTGCCAGGACAGCAGAAGATATGAAG
  CCACCCGTGTTATCGCTGATATGGAGAACTAGAGTTCTGAAGTCTCTGCTTCAGCAATCCCTTCAGGGA
  GTGGTGGGCTTTGGGATGGACCCTGACCTTCAGATGGATATCGTCACCGAGCTTGACCTTGTGAACACC
  ACCCTTGGAGTTGCTCAGGTGTCTGGAATGCACAATGCCAGCAAAGCATTTTTATTTCAAGACATAGAA
  AGAGAGATCCATGCAGCTCCTCATGTGAGTGAGAAATTAATTCAGCTGTTCCGGAACAAGAGTGAATTC
  ACCATTTTGGCCACTGTACAGCAGAAGCCATCCACTTCAGGAGTGATACTGTCCATTCGAGAACTGGAG
  CACAGCTATTTTGAACTGGAGAGCAGTGGCCTGAGGGATGAGATTCGGTATCACTACATACACAATGGG
  AAGCCAAGGACAGAGGCACTTCCTTACCGCATGGCAGATGGACAATGGCACAAGGTTGCACTGTCAGTT
  AGCGCCTCTCATCTCCTGCTCCATGTCGACTGTAACAGGATTTATGAGCGTGTGATAGACCCTCCAGAT
  ACCAACCTTCCCCCAGGAATCAATTTATGGCTTGGCCAGCGCAACCAAAAGCATGGCTTATTCAAAGGG
  ATCATCCAAGATGGGAAGATCATCTTTATGCCGAATGGATATATAACACAGTGTCCAAATCTAAATCAC
  ACTTGCCCAACCTGCAGTGATTTCTTAAGCCTGGTGCAAGGAATAATGGATTTACAAGAGCTTTTGGCC
  AAGATGACTGCAAAACTAAATTATGCAGAGACAAGACTTAGTCAATTGGAAAACTGTCATTGTGAGAAG
  ACTTGTCAAGTGAGTGGACTGCTCTATCGAGATCAAGACTCTTGGGTAGATGGTGACCATTGCAGGAAC
  TGCACTTGCAAAAGTGGTGCCGTGGAATGCCGAAGGATGTCCTGTCCCCCTCTCAATTGCTCCCCAGAC
  TCCCTCCCAGTGCACATTGCTGGCCAGTGCTGTAAGGTCTGCCGACCAAAATGTATCTATGGAGGAAAA
  GTTCTTGCAGAAGGCCAGCGGATTTTAACCAAGAGCTGTCGGGAATGCCGAGGTGGAGTTTTAGTAAAA
  ATTACAGAAATGTGTCCTCCTTTGAACTGCTCAGAAAAGGATCACATTCTTCCTGAGAATCAGTGCTGC
  CGTGTCTGTAGAGGTCATAACTTTTGTGCAGAAGGACCTAAATGTGGTGAAAACTCAGAGTGCAAAAAC
  TGGAATACAAAAGCTACTTGTGAGTGCAAGAGTGGTTACATCTCTGTCCAGGGAGACTCTGCCTACTGT
  GAAGATATTGATGAGTGTGCAGCTAAGATGCATTACTGTCATGCCAATACTGTGTGTGTCAACCTTCCT
  GGGTTATATCGCTGTGACTGTGTCCCAGGATACATTCGTGTGGATGACTTCTCTTGTACAGAACACGAT
  GAATGTGGCAGCGGCCAGCACAACTGTGATGAGAATGCCATCTGCACCAACACTGTCCAGGGACACAGC
  TGCACCTGCAAACCGGGCTACGTGGGGAACGGGACCATCTGCAGAGCTTTCTGTGAAGAGGGCTGCAGA
  TACGGTGGAACGTGTGTGGCTCCCAACAAATGTGTCTGTCCATCTGGATTCACAGGAAGCCACTGCGAG
  AAAGATATTGATGAATGTTCAGAGGGAATCATTGAGTGCCACAACCATTCCCGCTGCGTTAACCTGCCA
  GGGTGGTACCACTGTGAGTGCAGAAGCGGTTTCCATGACGATGGGACCTATTCACTGTCCGGGGAGTCC
  TGTATTGACATTGATGAATGTGCCTTAAGAACTCACACCTGTTGGAACGATTCTGCCTGCATCAACCTG
  GCAGGGGGCTTTGACTGTCTCTGCCCCTCTGGGCCCTCCTGCTCTGGTGACTGTCCTCATGAAGGGGGG
  CTGAAGCACAATGGCCAGGTGTGGACCTTGAAAGAAGACAGGTGTTCTGTCTGCTCCTGCAAGGATGGC
  AAGATATTCTGCCGACGGACAGCTTGTGATTGCCAGAATCCAAGTGCTGACCTATTCTGTTGCCCAGAA
  TGTGACACCAGAGTCACAAGTCAATGTTTAGACCAAAATGGTCACAAGCTGTATCGAAGTGGAGACAAT
  TGGACCCATAGCTGTCAGCAGTGTCGGTGTCTGGAAGGAGAGGTAGATTGCTGGCCACTCACTTGCCCC
  AACTTGAGCTGTGAGTATACAGCTATCTTAGAAGGGGAATGTTGTCCCCGCTGTGTCAGTGACCCCTGC
  CTAGCTGATAACATCACCTATGACATCAGAAAAACTTGCCTGGACAGCTATGGTGTTTCACGGCTTAGT
  GGCTCAGTGTGGACGATGGCTGGATCTCCCTGCACAACCTGTAAATGCAAGAATGGAAGAGTCTGTTGT
  TCTGTGGATTTTGAGTGTCTTCAAAATAAT
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG239659
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MPMDLILVVWFCVCTARTAEDMKPPVLSLIWRTRVLKSLLQQSLQGVVGFGMDPDLQMDIVTELDLVNT
  TLGVAQVSGMHNASKAFLFQDIEREIHAAPHVSEKLIQLFRNKSEFTILATVQQKPSTSGVILSIRELE
  HSYFELESSGLRDEIRYHYIHNGKPRTEALPYRMADGQWHKVALSVSASHLLLHVDCNRIYERVIDPPD
  TNLPPGINLWLGQRNQKHGLFKGIIQDGKIIFMPNGYITQCPNLNHTCPTCSDFLSLVQGIMDLQELLA
  KMTAKLNYAETRLSQLENCHCEKTCQVSGLLYRDQDSWVDGDHCRNCTCKSGAVECRRMSCPPLNCSPD
  SLPVHIAGQCCKVCRPKCIYGGKVLAEGQRILTKSCRECRGGVLVKITEMCPPLNCSEKDHILPENQCC
  RVCRGHNFCAEGPKCGENSECKNWNTKATCECKSGYISVQGDSAYCEDIDECAAKMHYCHANTVCVNLP
  GLYRCDCVPGYIRVDDFSCTEHDECGSGQHNCDENAICTNTVQGHSCTCKPGYVGNGTICRAFCEEGCR
  YGGTCVAPNKCVCPSGFTGSHCEKDIDECSEGIIECHNHSRCVNLPGWYHCECRSGFHDDGTYSLSGES
  CIDIDECALRTHTCWNDSACINLAGGFDCLCPSGPSCSGDCPHEGGLKHNGQVWTLKEDRCSVCSCKDG
  KIFCRRTACDCQNPSADLFCCPECDTRVTSQCLDQNGHKLYRSGDNWTHSCQQCRCLEGEVDCWPLTCP
  NLSCEYTAILEGECCPRCVSDPCLADNITYDIRKTCLDSYGVSRLSGSVWTMAGSPCTTCKCKNGRVCC
  SVDFECLQNN
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001288713
• ORF Size: 2514 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001288713.1, NP_001275642.1
• RefSeq Size: 3354 bp
• RefSeq ORF: 2517 bp
• Locus ID: 4745
• Cytogenetics: 11p15.1
• Protein Families: Secreted Protein
• MW: 93.3 kDa
• Gene Summary: 'This gene encodes a cytoplasmic protein that contains epidermal growth factor (EGF)-like repeats. The encoded heterotrimeric protein may be involved in cell growth regulation and differentiation. A similar protein in rodents is involved in craniosynostosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013]'