PIGG (NM_001289052) Human Tagged ORF Clone

CAT#: RG239676

PIGG (GFP-tagged) - Human phosphatidylinositol glycan anchor biosynthesis, class G (PIGG), transcript variant 4

Specifications

Product Data

• Tag: TurboGFP
• Symbol: PIGG
• Synonyms: GPI7; LAS21; MRT53; PRO4405; RLGS1930
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG239676 representing NM_001289052.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGCGGCTGGGCTCCGGGACTTTCGCTACCTGTTGCGTAGCGATCGAGGTGCTAGGGATCGCGGTCTTC
  CTTCGGGGATTCTTCCCGGCTCCCGTTCGTTCCTCTGCCAGAGCGGAACACGGAGCGGAGCCCCCAGCG
  CCCGAACCCTCGGCTGGAGCCAGTTCTAACTGGACCACGCTGCCACCACCTCTCTTCAGTAAAGTTGTT
  ATTGTTCTGATAGATGCCTTGAGAGATGATTTTGTGTTTGGGTCAAAGGGTGTGAAATTTATGCCCTAC
  ACAACTTACCTTGTGGAAAAAGGAGCATCTCACAGTTTTGTGGCTGAAGCAAAGCCACCTACAGTTACT
  ATGCCTCGAATCAAGGAGAGAGAGACGCCTTTACCCAATTTGCTGGTTCTTTGTGGTGACCATGGCATG
  TCTGAAACAGGAAGTCACGGGGCCTCCTCCACCGAGGAGGTGAATACACCTCTGATTTTAATCAGTTCT
  GCGTTTGAAAGGAAACCCGGTGATATCCGACATCCAAAGCACGTCCAACAGACGGATGTGGCTGCGACA
  CTGGCGATAGCACTTGGCTTACCGATTCCAAAAGACAGTGTAGGGAGCCTCCTATTCCCAGTTGTGGAA
  GGAAGACCAATGAGAGAGCAGTTGAGATTTTTACATTTGAATACAGTGCAGCTTAGTAAACTGTTGCAA
  GAGAATGTGCCGTCATATGAAAAAGATCCTGGGTTTGAGCAGTTTAAAATGTCAGAAAGATTGCATGGG
  AACTGGATCAGACTGTACTTGGAGGAAAAGCATTCAGAAGTCCTATTCAACCTGGGCTCCAAGGTTCTC
  AGGCAGTACCTGGATGCTCTGAAGACGCTGAGCTTGTCCCTGAGTGCACAAGTGGCCCAGTACGACATC
  TATTCGATGATGGTGGGGACTGTCGTGGTTTTGGAGGTTCTCACCCTGCTCCTGCTCAGCGTCCCACAG
  GCACTGCGCAGAAAGGCTGAGCTGGAAGTCCCACTGTCATCTCCTGGGTTTTCTCTGCTCTTTTATTTG
  GTGATCCTGGTTCTTTCGGCCGTTCACGTCATTGTGTGCACCTCAGCTGAAAGTTCGTGCTACTTCTGT
  GGCCTCTCGTGGCTGGCGGCAGGTGGGGTGATGGTGCTGGCCTCGGCGCTGCTGTGTGTGATTGTGTCT
  GTTCTGACCAACGTGCTCGTGGGTGGAAACACCCCAAGGAAGAACCCCATGCATCCCAGCTCAAGGTGG
  TCAGAGCTAGACCTTCTTATTCTGTTGGGGACGGCGGGCCACGTCTTGAGCCTGGGCGCCAGCAGCTTC
  GTGGAGGAGGAGCACCAGACCTGGTACTTCCTTGTGAACACCCTGTGTCTAGCTCTGAGCCAAGAAACC
  TACAGAAACTACTTTCTGGGAGATGACGGTGAGCCTCCGTGTGGCCTCTGTGTGGAACAAGGGCATGAC
  GGGGCCACAGCAGCGTGGCAGGACGGGCCTGGCTGTGATGTCCTGGAGCGAGACAAAGGCCACGGAAGC
  CCCTCTACCTCCGAAGTGCTCAGAGGCCGCGAGAAGTGGATGGTGCTGGCCAGTCCGTGGCTAATACTG
  GCCTGCTGCCGGCTGCTGCGCTCCCTAAACCAGACAGGTGTGCAGTGGGCTCACCGGCCTGACCTCGGC
  CACTGGCTCACCAGCTCTGACCACAAAGCCGAGCTCTCTGTCCTGGCTGCCCTCTCCCTCCTCGTAGTT
  TTTGTGCTGGTGCAGAGGGGGTGCTCCCCTGTGTCCAAGGCTGCCCTGGCGCTGGGGCTGCTGGGCGTC
  TACTGCTACCGGGCGGCCATCGGGAGTGTCCGGTTCCCGTGGCGGCCGGACAGCAAGGACATTTCCAAG
  GGTATTATTGAAGCTCGTTTTGTTTATGTCTTTGTCCTTGGCATTCTGTTCACGGGCACCAAAGACTTA
  CTTAAATCTCAAGTCATTGCTGCAGACTTCAAACTCAAGACTGTAGGTTTATGGGAGATATATAGTGGA
  TTAGTTCTTCTGGCAGCCTTGCTCTTTAGACCACATAATCTTCCGGTCTTAGCATTTAGCCTCTTGATT
  CAGACTCTAATGACTAAATTCATCTGGAAGCCCCTGAGACACGATGCAGCTGAGATTACTGTGATGCAT
  TATTGGTTTGGTCAAGCATTCTTCTATTTTCAGGGCAACTCCAACAACATTGCCACCGTGGACATCTCC
  GCAGGCTTCGTGGGCTTAGACACCTACGTGGAAATCCCAGCCGTGCTCCTGACAGCGTTTGGGACGTAC
  GCAGGGCCTGTGCTGTGGGCCAGCCACTTAGTGCACTTCCTGAGCTCAGAAACACGCAGTGGTTCAGCA
  CTGAGTCATGCTTGCTTCTGCTACGCACTGATTTGTTCTATTCCAGTTTTCACGTACATCGTTTTGGTG
  ACATCTCTGCGTTATCATTTATTTATATGGAGTGTATTTTCTCCAAAACTTCTCTACGAGGGAATGCAC
  CTGCTCATTACAGCTGCTGTCTGTGTATTCTTCACGGCAATGGATCAAACCAGACTCACACAGTCT
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG239676
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MRLGSGTFATCCVAIEVLGIAVFLRGFFPAPVRSSARAEHGAEPPAPEPSAGASSNWTTLPPPLFSKVV
  IVLIDALRDDFVFGSKGVKFMPYTTYLVEKGASHSFVAEAKPPTVTMPRIKERETPLPNLLVLCGDHGM
  SETGSHGASSTEEVNTPLILISSAFERKPGDIRHPKHVQQTDVAATLAIALGLPIPKDSVGSLLFPVVE
  GRPMREQLRFLHLNTVQLSKLLQENVPSYEKDPGFEQFKMSERLHGNWIRLYLEEKHSEVLFNLGSKVL
  RQYLDALKTLSLSLSAQVAQYDIYSMMVGTVVVLEVLTLLLLSVPQALRRKAELEVPLSSPGFSLLFYL
  VILVLSAVHVIVCTSAESSCYFCGLSWLAAGGVMVLASALLCVIVSVLTNVLVGGNTPRKNPMHPSSRW
  SELDLLILLGTAGHVLSLGASSFVEEEHQTWYFLVNTLCLALSQETYRNYFLGDDGEPPCGLCVEQGHD
  GATAAWQDGPGCDVLERDKGHGSPSTSEVLRGREKWMVLASPWLILACCRLLRSLNQTGVQWAHRPDLG
  HWLTSSDHKAELSVLAALSLLVVFVLVQRGCSPVSKAALALGLLGVYCYRAAIGSVRFPWRPDSKDISK
  GIIEARFVYVFVLGILFTGTKDLLKSQVIAADFKLKTVGLWEIYSGLVLLAALLFRPHNLPVLAFSLLI
  QTLMTKFIWKPLRHDAAEITVMHYWFGQAFFYFQGNSNNIATVDISAGFVGLDTYVEIPAVLLTAFGTY
  AGPVLWASHLVHFLSSETRSGSALSHACFCYALICSIPVFTYIVLVTSLRYHLFIWSVFSPKLLYEGMH
  LLITAAVCVFFTAMDQTRLTQS
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001289052
• ORF Size: 2550 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001289052.1, NP_001275981.1
• RefSeq Size: 3241 bp
• RefSeq ORF: 2553 bp
• Locus ID: 54872
• Cytogenetics: 4p16.3
• Protein Families: Transmembrane
• Protein Pathways: Glycosylphosphatidylinositol(GPI)-anchor biosynthesis
• MW: 93.7 kDa
• Gene Summary: This gene encodes an enzyme involved in glycosylphosphatidylinositol-anchor biosynthesis. The encoded protein, which is localized to the endoplasmic reticulum, is involved in transferring ethanoloamine phosphate to mannose 2 of glycosylphosphatidylinositol species H7 to form species H8. Allelic variants of this gene have been associated with intellectual disability, hypotonia, and early-onset seizures. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2016]