RPS6KC1 (NM_001287219) Human Tagged ORF Clone

CAT#: RG239689

RPS6KC1 (GFP-tagged) - Human ribosomal protein S6 kinase, 52kDa, polypeptide 1 (RPS6KC1), transcript variant 4

Specifications

Product Data

• Tag: TurboGFP
• Symbol: RPS6KC1
• Synonyms: humS6PKh1; RPK118; RSKL1; S6K-delta-1; S6PKh1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG239689 representing NM_001287219.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGCTTCCAATCAAAATTCTCCCATTAGAACTTTTGGTCTCAATCTTTCTTCGGATTCTTCAGCACTA
  GGGGCTGTTGCTTCTGACAGTGAACAGAGCAAAACAGAAGAAGAACGGGAAAGTCGTAGCCTCTTTCCT
  GGCAGTTTAAAGCCGAAGCTTGGCAAGAGAGATTATTTGGAGAAAGCAGGAGAATTAATAAAGCTGGCT
  TTAAAAAAGGAAGAAGAAGACGACTATGAAGCTGCTTCTGATTTTTATAGGAAGGGAGTTGATTTACTC
  CTAGAAGGTGTTCAAGGAGAGTCAAGCCCTACCCGTCGAGAAGCTGTGAAGAGAAGAACAGCCGAGTAC
  CTCATGCGGGCAGAAAGTATCTCTAGTCTTTATGGGAAACCTCAGCTTGATGATGTATCTCAGGTTTTA
  CTTGTAATGGACACAAGGACAGAACAGACTTTCATTTTAAAAGGTCTAAGGAAAAGCAGTGAATACAGC
  AGGAACAGAAAGACCATCATCCCCCGCTGTGTGCCCAACATGGTGTGTCTGCATAAGTACATCATCTCT
  GAGGAGTCAGTATTTCTTGTGCTGCAGCATGCGGAAGGTGGCAAACTGTGGTCATATATCAGTAAATTT
  CTAAACAGAAGTCCTGAAGAAAGCTTTGACATCAAGGAAGTGAAAAAACCTACACTTGCAAAAGTTCAC
  CTGCAGCAGCCAACTTCTAGTCCTCAGGACAGCAGTAGCTTTGAATCCAGAGGAAGTGATGGTGGAAGC
  ATGCTTAAAGCTCTGCCTTTGAAGAGTAGTCTTACTCCAAGTTCTCAAGATGACAGCAACCAGGAAGAT
  GATGGCCAAGATAGCTCTCCAAAGTGGCCAGATTCTGGTTCAAGTTCAGAAGAAGAATGTACTACTAGT
  TATTTAACATTATGCAATGAATATGGGCAAGAAAAGATTGAACCAGGGTCTTTGAATGAGGAGCCCTTC
  ATGAAGACTGAAGGGAATGGTGTTGATACAAAAGCTATTAAAAGCTTCCCAGCACACCTTGCTGCTGAC
  AGTGACAGCCCCAGCACACAGCTGAGAGCTCACGAGCTGAAGTTCTTCCCCAACGATGACCCAGAAGCA
  GTTAGTTCTCCAAGAACATCAGATTCCCTCAGTAGATCAAAAAATAGCCCCATGGAATTCTTTAGGATA
  GACAGTAAGGATAGCGCAAGTGAACTCCTGGGACTTGACTTTGGAGAAAAATTGTATAGTCTAAAATCA
  GAACCTTTGAAACCATTCTTTACTCTTCCAGATGGAGACAGTGCTTCTAGGAGTTTTAATACTAGTGAA
  AGCAAGGTAGAGTTTAAAGCTCAGGACACCATTAGCAGGGGCTCAGATGACTCAGTGCCAGTTATTTCA
  TTTAAAGATGCTGCTTTTGATGATGTCAGTGGTACTGATGAAGGAAGACCTGATCTTCTTGTAAATTTA
  CCTGGTGAATTGGAGTCAACAAGAGAAGCTGCAGCAATGGGACCTACTAAGTTTACACAAACTAATATA
  GGGATAATAGAAAATAAACTCTTGGAAGCCCCTGATGTTTTATGCCTCAGGCTTAGTACTGAACAATGC
  CAAGCACATGAGGAGAAAGGCATAGAGGAACTGAGTGATCCCTCTGGGCCCAAATCCTATAGTATAACA
  GAGAAACACTATGCACAGGAGGATCCCAGGATGTTATTTGTAGCAGCTGTTGATCATAGTAGTTCAGGA
  GATATGTCTTTGTTACCCAGCTCAGATCCTAAGTTTCAAGGACTTGGAGTGGTTGAGTCAGCAGTAACT
  GCAAACAACACAGAAGAAAGCTTATTCCGTATTTGTAGTCCACTCTCAGGTGCTAATGAATATATTGCA
  AGCACAGACACTTTAAAAACAGAAGAAGTATTGCTGTTTACAGATCAGACTGATGATTTGGCTAAAGAG
  GAACCAACTTCTTTATTCCAGAGAGACTCTGAGACTAAGGGTGAAAGTGGTTTAGTGCTAGAAGGAGAC
  AAGGAAATACATCAGATTTTTGAGGACCTTGATAAAAAATTAGCACTAGCCTCCAGGTTTTACATCCCA
  GAGGGCTGCATTCAAAGATGGGCAGCTGAAATGGTGGTAGCCCTTGATGCTTTACATAGAGAGGGAATT
  GTGTGCCGCGATTTGAACCCAAACAACATCTTATTGAATGATAGAGGACACATTCAGCTAACGTATTTT
  AGCAGGTGGAGTGAGGTTGAAGATTCCTGTGACAGCGATGCCATAGAGAGAATGTACTGTGCCCCAGAG
  GTTGGAGCAATCACTGAAGAAACTGAAGCCTGTGATTGGTGGAGTTTGGGTGCTGTCCTCTTTGAACTT
  CTCACTGGCAAGACTCTGGTTGAATGCCATCCAGCAGGAATAAATACTCACACTACTTTGAACATGCCA
  GAATGTGTCTCTGAAGAGGCTCGCTCACTCATTCAACAGCTCTTGCAGTTCAATCCTCTGGAACGACTT
  GGTGCTGGAGTTGCTGGTGTTGAAGATATCAAATCTCATCCATTTTTTACCCCTGTGGATTGGGCAGAA
  CTGATGAGA
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG239689
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MASNQNSPIRTFGLNLSSDSSALGAVASDSEQSKTEEERESRSLFPGSLKPKLGKRDYLEKAGELIKLA
  LKKEEEDDYEAASDFYRKGVDLLLEGVQGESSPTRREAVKRRTAEYLMRAESISSLYGKPQLDDVSQVL
  LVMDTRTEQTFILKGLRKSSEYSRNRKTIIPRCVPNMVCLHKYIISEESVFLVLQHAEGGKLWSYISKF
  LNRSPEESFDIKEVKKPTLAKVHLQQPTSSPQDSSSFESRGSDGGSMLKALPLKSSLTPSSQDDSNQED
  DGQDSSPKWPDSGSSSEEECTTSYLTLCNEYGQEKIEPGSLNEEPFMKTEGNGVDTKAIKSFPAHLAAD
  SDSPSTQLRAHELKFFPNDDPEAVSSPRTSDSLSRSKNSPMEFFRIDSKDSASELLGLDFGEKLYSLKS
  EPLKPFFTLPDGDSASRSFNTSESKVEFKAQDTISRGSDDSVPVISFKDAAFDDVSGTDEGRPDLLVNL
  PGELESTREAAAMGPTKFTQTNIGIIENKLLEAPDVLCLRLSTEQCQAHEEKGIEELSDPSGPKSYSIT
  EKHYAQEDPRMLFVAAVDHSSSGDMSLLPSSDPKFQGLGVVESAVTANNTEESLFRICSPLSGANEYIA
  STDTLKTEEVLLFTDQTDDLAKEEPTSLFQRDSETKGESGLVLEGDKEIHQIFEDLDKKLALASRFYIP
  EGCIQRWAAEMVVALDALHREGIVCRDLNPNNILLNDRGHIQLTYFSRWSEVEDSCDSDAIERMYCAPE
  VGAITEETEACDWWSLGAVLFELLTGKTLVECHPAGINTHTTLNMPECVSEEARSLIQQLLQFNPLERL
  GAGVAGVEDIKSHPFFTPVDWAELMR
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001287219
• ORF Size: 2562 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001287219.2
• RefSeq Size: 4243 bp
• RefSeq ORF: 2565 bp
• Locus ID: 26750
• Cytogenetics: 1q32.3
• Protein Families: Druggable Genome, Protein Kinase
• MW: 94.9 kDa
• Gene Summary: Sphingosine kinase catalyzes the formation of sphingosine 1 phosphate, a lipid cellular messenger. The protein encoded by this gene can bind to sphingosine kinase and to phosphatidylinositol 3-phosphate, suggesting a role in sphingosine 1 phophate signaling. The encoded protein can also bind to peroxiredoxin-3 and may help transport it to mitochondria. [provided by RefSeq, Mar 2017]