Hsp105 (HSPH1) (NM_001286504) Human Tagged ORF Clone

CAT#: RG239699

HSPH1 (GFP-tagged) - Human heat shock 105kDa/110kDa protein 1 (HSPH1), transcript variant 3

Specifications

Product Data

• Tag: TurboGFP
• Symbol: HSPH1
• Synonyms: HSP105; HSP105A; HSP105B; NY-CO-25
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG239699 representing NM_001286504.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGCAACGGCGGCCGTTCTCCGGGGACCGGCTGCCCATTGGGTAGAATCTTTCCAGAAGGCTCGAGAA
  GAAGGAAGCGGAAGTGGCACGTGGAGGGGCCGGTGGAGGCGCCGGTCAGTCATATCATTTGGATCAAAA
  AATAGAACAATCGGAGTTGCAGCCAAAAATCAGCAAATCACTCATGCAAACAATACGGTGTCTAACTTC
  AAAAGATTTCATGGCCGAGCATTCAATGACCCCTTCATTCAAAAGGAGAAGGAAAACTTGAGTTACGAT
  TTGGTTCCATTGAAAAATGGTGGAGTTGGAATAAAGGTAATGTACATGGGTGAAGAACATCTATTTAGT
  GTGGAGCAGATAACAGCCATGTTGTTGACTAAGCTGAAGGAAACTGCTGAAAACAGCCTCAAGAAACCA
  GTAACAGATTGTGTTATTTCAGTCCCCTCCTTCTTTACAGATGCTGAGAGGCGATCTGTGTTAGATGCT
  GCACAGATTGTTGGCCTAAACTGTTTAAGACTTATGAATGACATGACAGCTGTTGCTTTGAATTACGGA
  ATTTATAAGCAGGATCTCCCAAGCCTGGATGAGAAACCTCGGATAGTGGTTTTTGTTGATATGGGACAT
  TCAGCTTTTCAAGTGTCTGCTTGTGCTTTTAACAAGGGAAAATTGAAGGTACTGGGAACAGCTTTTGAT
  CCTTTCTTAGGAGGAAAAAACTTCGATGAAAAGTTAGTGGAACATTTTTGTGCAGAATTTAAAACTAAG
  TACAAGTTGGATGCAAAATCCAAAATACGAGCACTCCTACGTCTGTATCAGGAATGTGAAAAACTGAAA
  AAGCTAATGAGCTCTAACAGCACAGACCTTCCACTGAATATCGAATGCTTTATGAATGATAAAGATGTT
  TCCGGAAAGATGAACAGGTCACAATTTGAAGAACTCTGTGCTGAACTTCTGCAAAAGATAGAAGTACCC
  CTTTATTCACTGTTGGAACAAACTCATCTCAAAGTAGAAGATGTGAGTGCAGTTGAGATTGTTGGAGGC
  GCTACACGAATTCCAGCTGTGAAGGAAAGAATTGCCAAATTCTTTGGAAAAGATATTAGCACAACACTC
  AATGCAGATGAAGCAGTAGCCAGAGGATGTGCATTACAGTGTGCAATACTTTCCCCGGCATTTAAAGTT
  AGAGAATTTTCCGTCACAGATGCAGTTCCTTTTCCAATATCTCTGATCTGGAACCATGATTCAGAAGAT
  ACTGAAGGTGTTCATGAAGTCTTTAGTCGAAACCATGCTGCTCCTTTCTCCAAAGTTCTCACCTTTCTG
  AGAAGGGGGCCTTTTGAGCTAGAAGCTTTCTATTCTGATCCCCAAGGAGTTCCATATCCAGAAGCAAAA
  ATAGGCCGCTTTGTAGTTCAGAATGTTTCTGCACAGAAAGATGGAGAAAAATCTAGAGTAAAAGTCAAA
  GTGCGAGTCAACACCCATGGCATTTTCACCATCTCTACGGCATCTATGGTGGAGAAAGTCCCAACTGAG
  GAGAATGAAATGTCTTCTGAAGCTGACATGGAGTGTCTGAATCAGAGACCACCAGAAAACCCAGACACT
  GATAAAAATGTCCAGCAAGACAACAGTGAAGCTGGAACACAGCCCCAGGTACAAACTGATGCTCAACAA
  ACCTCACAGTCTCCCCCTTCACCTGAACTTACCTCAGAAGAAAACAAAATCCCAGATGCTGACAAAGCA
  AATGAAAAAAAAGTTGACCAGCCTCCAGAAGCTAAAAAGCCCAAAATAAAGGTGGTGAATGTTGAGCTG
  CCTATTGAAGCCAACTTGGTCTGGCAGTTAGGGAAAGACCTTCTTAACATGTATATTGAGACAGAGGGT
  AAGATGATAATGCAAGATAAATTGGAAAAAGAAAGGAATGATGCTAAAAATGCAGTTGAGGAATATGTG
  TATGAGTTCAGAGACAAGCTGTGTGGACCATATGAAAAATTTATATGTGAGCAGGATCATCAAAATTTT
  TTGAGACTCCTCACAGAAACTGAAGACTGGCTGTATGAAGAAGGAGAGGACCAAGCTAAACAAGCATAT
  GTTGACAAGTTGGAAGAATTAATGAAAATTGGCACTCCAGTTAAAGTTCGGTTTCAGGAAGCTGAAGAA
  CGGCCAAAAATGTTTGAAGAACTAGGACAGAGGCTGCAGCATTATGCCAAGATAGCAGCTGACTTCAGA
  AATAAGGATGAGAAATACAACCATATTGATGAGTCTGAAATGAAAAAAGTGGAGAAGTCTGTTAATGAA
  GTGATGGAATGGATGAATAATGTCATGAATGCTCAGGCTAAAAAGAGTCTTGATCAGGATCCAGTTGTA
  CGTGCTCAGGAAATTAAAACAAAAATCAAGGAATTGAACAACACATGTGAACCCGTTGTAACACAACCG
  AAACCAAAAATTGAATCACCCAAACTGGAAAGAACTCCAAATGGCCCAAATATTGATAAAAAGGAAGAA
  GATTTAGAAGACAAAAACAATTTTGGTGCTGAACCTCCACATCAGAATGGTGAATGTTACCCTAATGAG
  AAAAATTCTGTTAATATGGACTTGGAC
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG239699
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MATAAVLRGPAAHWVESFQKAREEGSGSGTWRGRWRRRSVISFGSKNRTIGVAAKNQQITHANNTVSNF
  KRFHGRAFNDPFIQKEKENLSYDLVPLKNGGVGIKVMYMGEEHLFSVEQITAMLLTKLKETAENSLKKP
  VTDCVISVPSFFTDAERRSVLDAAQIVGLNCLRLMNDMTAVALNYGIYKQDLPSLDEKPRIVVFVDMGH
  SAFQVSACAFNKGKLKVLGTAFDPFLGGKNFDEKLVEHFCAEFKTKYKLDAKSKIRALLRLYQECEKLK
  KLMSSNSTDLPLNIECFMNDKDVSGKMNRSQFEELCAELLQKIEVPLYSLLEQTHLKVEDVSAVEIVGG
  ATRIPAVKERIAKFFGKDISTTLNADEAVARGCALQCAILSPAFKVREFSVTDAVPFPISLIWNHDSED
  TEGVHEVFSRNHAAPFSKVLTFLRRGPFELEAFYSDPQGVPYPEAKIGRFVVQNVSAQKDGEKSRVKVK
  VRVNTHGIFTISTASMVEKVPTEENEMSSEADMECLNQRPPENPDTDKNVQQDNSEAGTQPQVQTDAQQ
  TSQSPPSPELTSEENKIPDADKANEKKVDQPPEAKKPKIKVVNVELPIEANLVWQLGKDLLNMYIETEG
  KMIMQDKLEKERNDAKNAVEEYVYEFRDKLCGPYEKFICEQDHQNFLRLLTETEDWLYEEGEDQAKQAY
  VDKLEELMKIGTPVKVRFQEAEERPKMFEELGQRLQHYAKIAADFRNKDEKYNHIDESEMKKVEKSVNE
  VMEWMNNVMNAQAKKSLDQDPVVRAQEIKTKIKELNNTCEPVVTQPKPKIESPKLERTPNGPNIDKKEE
  DLEDKNNFGAEPPHQNGECYPNEKNSVNMDLD
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001286504
• ORF Size: 2580 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001286504.1, NP_001273433.1
• RefSeq Size: 5221 bp
• RefSeq ORF: 2583 bp
• Locus ID: 10808
• Cytogenetics: 13q12.3
• Protein Families: Stem cell - Pluripotency
• MW: 97.9 kDa
• Gene Summary: This gene encodes a member of the heat shock protein 70 family of proteins. The encoded protein functions as a nucleotide exchange factor for the molecular chaperone heat shock cognate 71 kDa protein (Hsc70). In addition, this protein plays a distinct but related role as a holdase that inhibits the aggregation of misfolded proteins, including the cystic fibrosis transmembrane conductance regulator (CFTR) protein. Elevated expression of this protein has been observed in numerous human cancers. [provided by RefSeq, Mar 2017]