TOP3B (NM_001282113) Human Tagged ORF Clone

CAT#: RG239704

TOP3B (GFP-tagged) - Human topoisomerase (DNA) III beta (TOP3B), transcript variant 3

Specifications

Product Data

• Tag: TurboGFP
• Symbol: TOP3B
• Synonyms: TOP3B1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG239704 representing NM_001282113.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGAAGACTGTGCTCATGGTTGCTGAAAAGCCGTCCTTGGCACAGTCAATTGCCAAAATCCTCTCTAGA
  GGGAGCCTGTCCTCACACAAAGGGCTGAACGGGGCCTGCTCAGTCCACGAGTACACTGGGACCTTTGCT
  GGCCAGCCAGTGCGCTTCAAGATGACGTCTGTCTGTGGTCACGTGATGACCCTGGATTTCCTGGGAAAA
  TACAACAAATGGGACAAAGTGGACCCCGCAGAACTGTTCAGCCAAGCTCCCACGGAGAAGAAAGAAGCT
  AACCCCAAGCTGAACATGGTGAAGTTCCTGCAGGTGGAGGGCAGAGGCTGCGACTACATCGTGCTGTGG
  CTGGACTGCGACAAGGAGGGGGAGAACATCTGCTTTGAGGTTCTTGATGCTGTTCTGCCCGTCATGAAC
  AAGGCCCATGGTGGCGAGAAGACCGTGTTCCGGGCCAGGTTTAGCTCCATCACGGACACAGACATCTGT
  AATGCCATGGCCTGCCTAGGCGAGCCTGACCACAACGAGGCGCTCTCAGTGGATGCTCGCCAGGAGCTG
  GACCTGCGAATCGGCTGTGCATTCACCAGGTTTCAGACTAAATATTTCCAGGGGAAATACGGTGATTTA
  GACAGCTCTCTCATCTCCTTTGGGCCGTGTCAGACTCCAACCCTGGGATTCTGTGTGGAGAGACATGAT
  AAAATCCAGTCCTTCAAACCAGAGACCTACTGGGTGCTGCAGGCCAAGGTTAACACTGACAAAGACAGA
  TCTCTCCTTTTGGACTGGGACCGAGTAAGAGTGTTTGACCGGGAGATCGCACAGATGTTTTTAAACATG
  ACAAAGCTGGAGAAGGAAGCCCAGGTGGAGGCCACAAGCAGGAAAGAAAAGGCCAAGCAGAGGCCCCTG
  GCCCTGAACACTGTGGAGATGCTGCGTGTGGCCAGCTCTTCTCTGGGCATGGGGCCGCAGCACGCCATG
  CAGACGGCTGAGCGGCTCTACACGCAAGGCTACATCAGCTACCCACGGACAGAGACCACCCACTACCCT
  GAGAACTTTGACCTGAAGGGCTCTCTGCGGCAGCAGGCCAACCACCCCTACTGGGCCGACACGGTGAAG
  CGGTTGTTAGCAGAAGGTATCAACCGCCCGCGGAAAGGCCATGACGCCGGCGACCATCCCCCCATCACC
  CCCATGAAGTCTGCCACAGAGGCCGAATTAGGGGGTGACGCGTGGCGGCTCTATGAGTACATCACCAGA
  CACTTCATCGCCACGGTCAGCCATGACTGCAAGTACCTGCAGAGCACCATCTCCTTCAGAATTGGGCCC
  GAGCTCTTCACCTGCTCCGGGAAGACCGTCCTCTCACCAGGCTTCACGGAGGTCATGCCCTGGCAGAGC
  GTGCCCCTGGAGGAGAGCCTGCCCACTTGCCAGCGGGGTGATGCCTTCCCTGTGGGCGAGGTGAAGATG
  CTGGAGAAGCAGACGAACCCACCCGACTACCTGACGGAGGCCGAGCTCATCACGCTCATGGAGAAGCAT
  GGCATCGGCACGGATGCCAGCATCCCTGTGCATATCAACAACATCTGCCAGCGCAACTATGTCACGGTG
  GAGAGCGGGCGCCGGCTCAAGCCCACCAACCTCGGCATCGTCCTGGTGCACGGCTACTATAAGATTGAT
  GCAGAGCTGGTGCTCCCCACCATCCGCAGTGCAGTGGAGAAGCAGCTGAACCTGATCGCCCAGGGCAAG
  GCCGACTACCGCCAGGTCCTGGGCCACACCCTGGACGTGTTCAAGAGGAAGTTCCACTACTTTGTCGAC
  TCCATTGCTGGCATGGATGAGTTGATGGAGGTGTCTTTCTCGCCCCTGGCGGCCACAGGCAAGCCCCTC
  TCACGCTGTGGGAAGTGCCACCGCTTCATGAAGTACATCCAGGCCAAGCCAAGCCGCCTGCACTGCTCC
  CACTGCGATGAGACCTACACGCTCCCCCAGAACGGCACCATCAAGCTCTACAAGGAGCTCCGCTGCCCT
  CTGGATGACTTCGAGCTGGTCCTGTGGTCATCAGGCTCTCGGGGCAAGAGCTACCCGCTGTGCCCCTAC
  TGCTACAACCACCCACCCTTCCGAGACATGAAGAAAGGCATGGGCTGCAACGAGTGTACGCACCCCTCC
  TGCCAGCACTCGCTGAGCATGCTGGGCATCGGCCAGTGCGTGGAATGTGAGAGCGGGGTGCTGGTGCTG
  GACCCCACCTCGGGCCCCAAGTGGAAGGTGGCCTGCAACAAGTGCAACGTGGTAGCGCACTGCTTCGAG
  AACGCCCACCGCGTGCGGGTGTCCGCCGACACCTGCAGTGTCTGTGAGGCCGCCTTGCTTGATGTGGAC
  TTCAACAAGGCCAAGTCCCCACTCCCGGGCGATGAGACGCAGCACATGGGCTGCGTCTTTTGTGACCCC
  GTCTTCCAGGAGCTGGTGGAGCTGAAGCATGCGGCCTCCTGCCACCCCATGCACCGCGGTGGACCAGGG
  AGAAGGCAGGGTCGAGGGCGGGGCCGGGCCAGGAGGCCCCCTGGGAAGCCCAACCCCAGACGGCCCAAG
  GACAAGATGTCAGCCCTGGCCGCCTACTTTGTA
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG239704
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MKTVLMVAEKPSLAQSIAKILSRGSLSSHKGLNGACSVHEYTGTFAGQPVRFKMTSVCGHVMTLDFLGK
  YNKWDKVDPAELFSQAPTEKKEANPKLNMVKFLQVEGRGCDYIVLWLDCDKEGENICFEVLDAVLPVMN
  KAHGGEKTVFRARFSSITDTDICNAMACLGEPDHNEALSVDARQELDLRIGCAFTRFQTKYFQGKYGDL
  DSSLISFGPCQTPTLGFCVERHDKIQSFKPETYWVLQAKVNTDKDRSLLLDWDRVRVFDREIAQMFLNM
  TKLEKEAQVEATSRKEKAKQRPLALNTVEMLRVASSSLGMGPQHAMQTAERLYTQGYISYPRTETTHYP
  ENFDLKGSLRQQANHPYWADTVKRLLAEGINRPRKGHDAGDHPPITPMKSATEAELGGDAWRLYEYITR
  HFIATVSHDCKYLQSTISFRIGPELFTCSGKTVLSPGFTEVMPWQSVPLEESLPTCQRGDAFPVGEVKM
  LEKQTNPPDYLTEAELITLMEKHGIGTDASIPVHINNICQRNYVTVESGRRLKPTNLGIVLVHGYYKID
  AELVLPTIRSAVEKQLNLIAQGKADYRQVLGHTLDVFKRKFHYFVDSIAGMDELMEVSFSPLAATGKPL
  SRCGKCHRFMKYIQAKPSRLHCSHCDETYTLPQNGTIKLYKELRCPLDDFELVLWSSGSRGKSYPLCPY
  CYNHPPFRDMKKGMGCNECTHPSCQHSLSMLGIGQCVECESGVLVLDPTSGPKWKVACNKCNVVAHCFE
  NAHRVRVSADTCSVCEAALLDVDFNKAKSPLPGDETQHMGCVFCDPVFQELVELKHAASCHPMHRGGPG
  RRQGRGRGRARRPPGKPNPRRPKDKMSALAAYFV
  SGPTRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLL
  SHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGF
  PEDSVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGP
  MFAFRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001282113
• ORF Size: 2586 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001282113.2
• RefSeq Size: 2901 bp
• RefSeq ORF: 2589 bp
• Locus ID: 8940
• Cytogenetics: 22q11.22
• Protein Pathways: Homologous recombination
• MW: 96.7 kDa
• Gene Summary: This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus relaxing the supercoils and altering the topology of DNA. The enzyme interacts with DNA helicase SGS1 and plays a role in DNA recombination, cellular aging and maintenance of genome stability. Low expression of this gene may be related to higher survival rates in breast cancer patients. This gene has a pseudogene on chromosome 22. Alternate splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Aug 2013]