PER3 (NM_001289864) Human Tagged ORF Clone

CAT#: RG239744

PER3 (GFP-tagged) - Human period circadian clock 3 (PER3), transcript variant 5

Specifications

Product Data

• Tag: TurboGFP
• Symbol: PER3
• Synonyms: FASPS3; GIG13
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG239744 representing NM_001289864.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGTTGCCATACACCAAAAAGTTTTGAAGTATGCAGGGCATCCTCCCTTTGAACATTCTCCCATTCGA
  TTTTGTACTCAAAACGGAGACTACATCATACTGGATTCCAGTTGGTCCAGCTTTGTGAATCCCTGGAGC
  CGGAAGATTTCTTTCATCATTGGTCGGCATAAAGTTCGAACGAGCCCACTAAATGAGGATGTTTTTGCT
  ACCAAAATTAAAAAGATGAACGATAATGACAAAGACATAACAGAATTACAAGAACAAATTTACAAACTT
  CTCTTACAGCCAGTTCACGTGAGCGTGTCCAGCGGCTACGGGAGCCTGGGGAGCAGCGGGTCGCAGGAG
  CAGCTTGTCAGCATCGCCTCCTCCAGTGAGGCCAGTGGGCACCGTGTGGAGGAGACGAAGGCGGAGCAG
  ATGACCTTGCAGCAGGTCTATGCCAGTGTGAACAAAATTAAAAATCTGGGTCAGCAGCTCTACATTGAG
  TCAATGACCAAATCATCATTCAAGCCAGTGACGGGGACACGCACAGAACCGAATGGTGGTGGTGAGTCA
  GCGAATGGTGGTGGTGAATGTAAGACCTTTACTTCCTTCCACCAAACACTGAAAAACAATAGTGTGTAC
  ACTGAGCCCTGTGAGGATTTGAGGAACGATGAGCACAGCCCATCCTATCAACAGATCAACTGTATCGAC
  AGTGTCATCAGATACCTGAAGAGCTACAACATTCCAGCTTTGAAAAGAAAGTGTATCTCCTGTACAAAT
  ACAACTTCTTCCTCCTCAGAAGAAGACAAACAGAACCACAAGGCAGATGATGTCCAAGCCTTACAAGCT
  GGTTTGCAAATCCCAGCCATACCTAAATCAGAAATGCCAACAAATGGACGGTCCATAGACACAGGAGGA
  GGAGCTCCACAGATCCTGTCCACGGCGATGCTGAGCTTGGGGTCGGGCATAAGCCAATGCGGTTACAGC
  AGCACCATTGTCCATGTCCCACCCCCAGAGACAGCCAGGGATGCTACCCTCTTCTGTGAGCCCTGGACC
  CTGAACATGCAGCCAGCCCCTTTGACCTCGGAAGAATTTAAACACGTGGGGCTCACAGCGGCTGTTCTG
  TCAGCGCACACCCAGAAGGAAGAGCAGAATTATGTTGATAAATTCCGAGAAAAGATCCTGTCATCACCC
  TACAGCTCCTATCTTCAGCAAGAAAGCAGGAGCAAAGCTAAATATTCATATTTTCAAGGAGATTCTACT
  TCCAAGCAGACGCGGTCGGCCGGCTGCAGGAAAGGGAAGCACAAGCGGAAGAAGCTGCCGGAGCCGCCA
  GACAGCAGCAGCTCGAACACCGGCTCTGGTCCCCGCAGGGGAGCGCATCAGAACGCACAGCCCTGCTGC
  CCCTCCGCGGCCTCCTCTCCGCACACCTCGAGCCCGACCTTCCCACCTGCCGCCATGGTGCCCAGCCAG
  GCCCCTTACCTCGTCCCAGCTTTTCCCCTCCCAGCCGCGACCTCACCCGGAAGAGAATACGCAGCCCCC
  GGAACTGCACCGGAAGGCCTGCATGGGCTGCCCTTGTCCGAGGGCTTGCAGCCTTACCCAGCTTTCCCT
  TTTCCTTACTTGGATACTTTTATGACCGTTTTCCTGCCTGACCCCCCTGTCTGTCCTCTGTTGTCGCCA
  TCGTTTTTGCCATGTCCATTCCTGGGGGCGACAGCCTCTTCTGCGATATCACCCTCAATGTCGTCAGCA
  ATGAGTCCAACTCTGGACCCACCCCCTTCAGTCACCAGCCAAAGGAGAGAGGAGGAAAAGTGGGAGGCA
  CAAAGCGAGGGGCACCCGTTCATTACTTCGAGAAGCAGCTCACCCTTGCAGTTAAACTTACTTCAGGAA
  GAGATGCCCAGACCCTCTGAATCTCCAGATCAGATGAGAAGGAACACGTGCCCACAAACTGAGTATTGT
  GTTACAGGCAACAATGGCAGTGAGAGCAGTCCTGCTACTACCGGTGCACTGTCCACGGGGTCACCTCCC
  AGGGAGAATCCATCCCATCCTACTGCCAGCGCTCTGTCCACAGGATCGCCTCCCATGAAGAATCCATCC
  CATCCTACTGCCAGCGCTCTGTCCACAGGATCGCCTCCCATGAAGAATCCATCCCATCCTACTGCCAGC
  ACACTGTCCATGGGATTGCCTCCCAGCAGGACTCCATCCCATCCTACTGCCACTGTTCTGTCCACGGGG
  TCACCTCCCAGCGAATCCCCATCCAGAACTGGTTCAGCAGCATCAGGAAGCAGCGACAGCAGTATATAC
  CTTACTAGTAGTGTTTATTCTTCTAAAATCTCCCAAAATGGGCAGCAATCTCAGGACGTACAGAAAAAA
  GAAACATTTCCTAATGTCGCCGAAGAGCCCATCTGGAGAATGATACGGCAGACACCTGAGCGCATTCTC
  ATGACATACCAGGTACCTGAGAGGGTTAAAGAAGTTGTACTAAAAGAAGACCTGGAAAAGCTAGAAAGT
  ATGAGGCAGCAGCAGCCCCAGTTTTCTCATGGGCAAAAGGAGGAGCTGGCTAAGGTGTATAATTGGATT
  CAAAGCCAGACTGTCACTCAAGAAATCGACATTCAAGCCTGTGTCACTTGTGAAAATGAAGATTCAGCT
  GATGGTGCGGCCACATCCTGTGGTCAGGTTCTGGTAGAAGACAGCTGT
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG239744
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MVAIHQKVLKYAGHPPFEHSPIRFCTQNGDYIILDSSWSSFVNPWSRKISFIIGRHKVRTSPLNEDVFA
  TKIKKMNDNDKDITELQEQIYKLLLQPVHVSVSSGYGSLGSSGSQEQLVSIASSSEASGHRVEETKAEQ
  MTLQQVYASVNKIKNLGQQLYIESMTKSSFKPVTGTRTEPNGGGESANGGGECKTFTSFHQTLKNNSVY
  TEPCEDLRNDEHSPSYQQINCIDSVIRYLKSYNIPALKRKCISCTNTTSSSSEEDKQNHKADDVQALQA
  GLQIPAIPKSEMPTNGRSIDTGGGAPQILSTAMLSLGSGISQCGYSSTIVHVPPPETARDATLFCEPWT
  LNMQPAPLTSEEFKHVGLTAAVLSAHTQKEEQNYVDKFREKILSSPYSSYLQQESRSKAKYSYFQGDST
  SKQTRSAGCRKGKHKRKKLPEPPDSSSSNTGSGPRRGAHQNAQPCCPSAASSPHTSSPTFPPAAMVPSQ
  APYLVPAFPLPAATSPGREYAAPGTAPEGLHGLPLSEGLQPYPAFPFPYLDTFMTVFLPDPPVCPLLSP
  SFLPCPFLGATASSAISPSMSSAMSPTLDPPPSVTSQRREEEKWEAQSEGHPFITSRSSSPLQLNLLQE
  EMPRPSESPDQMRRNTCPQTEYCVTGNNGSESSPATTGALSTGSPPRENPSHPTASALSTGSPPMKNPS
  HPTASALSTGSPPMKNPSHPTASTLSMGLPPSRTPSHPTATVLSTGSPPSESPSRTGSAASGSSDSSIY
  LTSSVYSSKISQNGQQSQDVQKKETFPNVAEEPIWRMIRQTPERILMTYQVPERVKEVVLKEDLEKLES
  MRQQQPQFSHGQKEELAKVYNWIQSQTVTQEIDIQACVTCENEDSADGAATSCGQVLVEDSC
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001289864
• ORF Size: 2670 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001289864.3
• RefSeq Size: 6394 bp
• RefSeq ORF: 2673 bp
• Locus ID: 8863
• Cytogenetics: 1p36.23
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Circadian rhythm - mammal
• MW: 96.8 kDa
• Gene Summary: This gene is a member of the Period family of genes and is expressed in a circadian pattern in the suprachiasmatic nucleus, the primary circadian pacemaker in the mammalian brain. Genes in this family encode components of the circadian rhythms of locomotor activity, metabolism, and behavior. This gene is upregulated by CLOCK/ARNTL heterodimers but then represses this upregulation in a feedback loop using PER/CRY heterodimers to interact with CLOCK/ARNTL. Polymorphisms in this gene have been linked to sleep disorders. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2014]