DGKH (NM_001297429) Human Tagged ORF Clone

CAT#: RG239785

DGKH (GFP-tagged) - Human diacylglycerol kinase, eta (DGKH), transcript variant 6

Specifications

Product Data

• Tag: TurboGFP
• Symbol: DGKH
• Synonyms: DGKeta
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG239785 representing NM_001297429.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGTGGAACATTTCTCAGGGATGCACAACTGGTACGCCTGCTCCCACGCCCGACCCACCTTCTGTAACG
  TGTGCAGAGAGAGTCTTTCTGGAGTCACCTCCCATGGCCTGTCCTGCGAAGGTACACACTGCCTGCAAA
  GATTTATACCATCCAATATGTCCACTTGGTCAATGTAAAGTATCTATCATACCTCCAATTGCACTAAAC
  AGCACCGATTCCGATGGTTTCTGTAGAGCAACATTTTCGTTCTGTGTTAGTCCTCTATTGGTTTTTGTC
  AATTCTAAGAGTGGAGATAATCAGGGAGTAAAGTTCCTCCGTCGCTTTAAACAGTTGCTAAATCCGGCT
  CAGGTGTTTGATTTAATGAATGGAGGTCCTCATTTAGGTTTAAGATTATTTCAGAAGTTTGACAATTTC
  CGGATTCTTGTTTGTGGAGGCGATGGAAGTGTAGGTTGGGTTTTGTCAGAAATCGATAAGCTCAACTTG
  AATAAACAGTGTCAGCTGGGAGTGTTGCCTTTGGGTACAGGAAATGACCTTGCCCGAGTTCTTGGCTGG
  GGAGGTTCATATGACGATGACACCCAACTTCCTCAGATCCTAGAGAAACTGGAACGAGCCAGTACCAAA
  ATGTTGGACAGGTGGAGTATAATGACATATGAACTCAAATTGCCACCAAAAGCTTCCCTACTTCCAGGA
  CCTCCAGAAGCATCTGAAGAATTTTATATGACGATTTATGAAGACTCAGTTGCAACGCATCTTACAAAA
  ATCCTCAATTCTGATGAACATGCAGTGGTCATATCTTCTGCCAAGACGCTATGTGAAACTGTAAAGGAC
  TTCGTTGCCAAAGTAGAAAAGACGTATGACAAAACCTTGGAAAATGCCGTTGTAGCTGATGCCGTGGCC
  AGTAAATGTTCAGTCCTAAACGAGAAGCTCGAACAACTGCTGCAGGCTTTGCACACAGATTCCCAGGCT
  GCGCCTGTTCTCCCTGGCCTCAGCCCTCTCATTGTGGAAGAAGATGCTGTGGAATCGTCCAGTGAAGAG
  TCCCTGGGTGAAAGCAAAGAGCAGCTTGGGGATGACGTTACAAAACCTTCCTCCCAGAAAGCCGTCAAA
  CCAAGGGAAATCATGTTGCGGGCAAATAGTTTAAAGAAAGCAGTGAGGCAAGTCATTGAGGAAGCCGGA
  AAAGTTATGGATGACCCGACAGTTCACCCCTGTGAACCAGCTAATCAGTCCTCTGATTATGACAGCACA
  GAAACAGATGAATCTAAGGAGGAAGCTAAAGATGATGGTGCCAAAGAATCAATAACTGTTAAAACTGCA
  CCTCGGTCTCCAGATGCCCGGGCAAGTTATGGCCATTCCCAAACTGATTCTGTCCCTGGTCCAGCTGTG
  GCAGCCAGCAAAGAAAACCTCCCTGTGCTCAATACCAGAATAATCTGCCCAGGTTTAAGAGCAGGACTG
  GCTGCCTCAATTGCTGGGAGTTCGATTATCAACAAAATGTTACTGGCAAACATTGATCCTTTTGGTGCC
  ACGCCGTTTATTGACCCGGATCTAGATTCCGTAGATGGATATTCAGAAAAATGTGTCATGAACAATTAC
  TTTGGGATTGGATTAGATGCAAAAATTTCATTAGAATTTAATAATAAAAGAGAGGAGCACCCTGAAAAA
  TGCAGGAGCCGAACTAAAAACTTGATGTGGTATGGAGTCCTTGGAACCCGGGAGTTATTACAGAGATCG
  TACAAGAATTTAGAACAAAGGGTTCAACTTGAGTGTGATGGGCAGTATATTCCTCTTCCCAGCTTGCAA
  GGCATAGCCGTGTTGAACATTCCCAGCTATGCTGGAGGCACTAACTTTTGGGGTGGAACTAAAGAGGAT
  GATATATTTGCTGCACCATCCTTTGATGACAAGATCCTGGAAGTTGTAGCAATATTTGATAGCATGCAA
  ATGGCAGTTTCAAGGGTCATTAAACTGCAGCATCATCGAATAGCCCAGTGCCGTACAGTGAAAATCACT
  ATATTTGGTGACGAAGGAGTCCCAGTGCAAGTGGATGGTGAAGCGTGGGTTCAGCCTCCAGGGATTATC
  AAAATTGTGCACAAAAACAGAGCACAAATGCTAACAAGGGACAGAGCCTTTGAGAGCACTCTGAAATCT
  TGGGAAGATAAGCAGAAGTGTGATTCTGGTAAACCAGTTCTCCGAACCCATTTGTACATCCATCACGCC
  ATTGACTTGGCAACAGAAGAGGTGTCGCAGATGCAGCTATGCTCCCAGGCTGCAGAGGAGCTCATTACT
  AGGATATGTGACGCAGCCACAATTCACTGTCTTTTGGAGCAAGAACTGGCCCATGCTGTGAATGCCTGC
  TCCCATGCCCTGAATAAAGCCAACCCAAGGTGCCCGGAGAGTCTTACAAGAGACACTGCCACTGAAATA
  GCCATCAATGTGAAGGCGCTGTATAATGAAACAGAATCTTTGCTAGTTGGCAGGGTTCCTTTGCAGCTG
  GAATCGCCACATGAAGAGCGAGTATCCAATGCCTTACACTCTGTGGAGGTGGAATTACAGAAACTGACA
  GAGATTCCTTGGCTTTATTATATCTTACACCCAAATGAGGATGAGGAACCTCCTATGGATTGCACCAAA
  AGGAACAACAGAAGCACCGTATTTCGAATAGTGCCAAAGTTTAAAAAGGAAAAGGTTCAGAAGCAGAAG
  ACAAGTTCACAGCCTGGATCTGGGGATACCGAAAGTGGGTCATGTGAAGCGAATTCTCCAGGGAAT
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG239785
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MWNISQGCTTGTPAPTPDPPSVTCAERVFLESPPMACPAKVHTACKDLYHPICPLGQCKVSIIPPIALN
  STDSDGFCRATFSFCVSPLLVFVNSKSGDNQGVKFLRRFKQLLNPAQVFDLMNGGPHLGLRLFQKFDNF
  RILVCGGDGSVGWVLSEIDKLNLNKQCQLGVLPLGTGNDLARVLGWGGSYDDDTQLPQILEKLERASTK
  MLDRWSIMTYELKLPPKASLLPGPPEASEEFYMTIYEDSVATHLTKILNSDEHAVVISSAKTLCETVKD
  FVAKVEKTYDKTLENAVVADAVASKCSVLNEKLEQLLQALHTDSQAAPVLPGLSPLIVEEDAVESSSEE
  SLGESKEQLGDDVTKPSSQKAVKPREIMLRANSLKKAVRQVIEEAGKVMDDPTVHPCEPANQSSDYDST
  ETDESKEEAKDDGAKESITVKTAPRSPDARASYGHSQTDSVPGPAVAASKENLPVLNTRIICPGLRAGL
  AASIAGSSIINKMLLANIDPFGATPFIDPDLDSVDGYSEKCVMNNYFGIGLDAKISLEFNNKREEHPEK
  CRSRTKNLMWYGVLGTRELLQRSYKNLEQRVQLECDGQYIPLPSLQGIAVLNIPSYAGGTNFWGGTKED
  DIFAAPSFDDKILEVVAIFDSMQMAVSRVIKLQHHRIAQCRTVKITIFGDEGVPVQVDGEAWVQPPGII
  KIVHKNRAQMLTRDRAFESTLKSWEDKQKCDSGKPVLRTHLYIHHAIDLATEEVSQMQLCSQAAEELIT
  RICDAATIHCLLEQELAHAVNACSHALNKANPRCPESLTRDTATEIAINVKALYNETESLLVGRVPLQL
  ESPHEERVSNALHSVEVELQKLTEIPWLYYILHPNEDEEPPMDCTKRNNRSTVFRIVPKFKKEKVQKQK
  TSSQPGSGDTESGSCEANSPGN
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001297429
• ORF Size: 2757 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001297429.2
• RefSeq Size: 16746 bp
• RefSeq ORF: 2760 bp
• Locus ID: 160851
• Cytogenetics: 13q14.11
• Protein Families: Druggable Genome
• Protein Pathways: Glycerolipid metabolism, Glycerophospholipid metabolism, Metabolic pathways, Phosphatidylinositol signaling system
• MW: 101.5 kDa
• Gene Summary: This gene encodes a member of the diacylglycerol kinase (DGK) enzyme family. Members of this family are involved in regulating intracellular concentrations of diacylglycerol and phosphatidic acid. Variation in this gene has been associated with bipolar disorder. Alternatively spliced transcript variants have been identified. [provided by RefSeq, Jul 2014]