AASDH (NM_001286669) Human Tagged ORF Clone

CAT#: RG239811

AASDH (GFP-tagged) - Human aminoadipate-semialdehyde dehydrogenase (AASDH), transcript variant 3

Specifications

Product Data

• Tag: TurboGFP
• Symbol: AASDH
• Synonyms: ACSF4; LYS2; NRPS998; NRPS1098
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG239811 representing NM_001286669.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGCTAAATGATGGAAAAGAGAAATATGAAAAAGAAAAAATAAAAAGCATAAGTTCTGAGCATGTCAAT
  GAAGAAAAAGCAGAAGAACACATGGATCTGAGGCTAAAGCATTGCTTAGCCTATGTTCTACATACATCA
  GGGACTACAGGGATACCGAAGATTGTCAGAGTGCCTCATAAGTGTATAGTACCAAATATCCAGCATTTT
  CGGGTACTTTTTGACATCACACAAGAAGATGTTTTGTTTCTGGCTTCACCTCTGACCTTCGATCCTTCT
  GTTGTGGAAATATTTCTTGCTCTATCAAGTGGTGCCTCTCTGCTTATTGTACCAACTTCCGTCAAGTTG
  CTCCCATCAAAATTAGCCAGCGTTCTCTTTTCCCATCATAGAGTGACTGTTTTGCAGGCAACACCAACA
  TTGCTTAGAAGATTTGGATCTCAGCTTATCAAGTCAACTGTTTTGTCAGCCACTACTTCTCTTCGAGTA
  TTAGCCCTTGGTGGTGAAGCGTTTCCATCATTGACAGTTCTCAGAAGCTGGAGAGGAGAAGGCAATAAA
  ACACAAATATTTAATGTTTATGGTATCACAGAGGTATCAAGTTGGGCGACCATTTATAGGATTCCAGAG
  AAGACTCTTAACTCTACTCTCAAATGTGAATTGCCTGTACAACTGGGATTTCCACTTCTTGGAACAGTA
  GTTGAAGTCAGAGATACTAATGGCTTCACAATTCAGGAAGGCAGTGGCCAAGTATTTTTAGGTGGCAGA
  AACAGAGTGTGTTTTCTTGATGATGAAGTGACAGTACCACTTGGCACAATGCGAGCTACAGGAGACTTT
  GTGACTGTGAAAGATGGAGAGATTTTTTTTTTGGGACGAAAAGACAGTCAGATCAAACGTCATGGCAAA
  CGTCTTAACATTGAACTTGTGCAACAGGTTGCTGAAGAGCTTCAGCAAGTGGAGTCTTGTGCAGTTACA
  TGGTATAATCAGGAAAAATTAATTCTCTTCATGGTGTCTAAAGATGCTTCAGTAAAAGAATACATCTTT
  AAAGAACTGCAGAAATATCTTCCAAGTCATGCAGTCCCGGATGAGCTTGTATTGATCGACTCTCTACCA
  TTTACATCCCACGGCAAAATTGATGTTTCTGAGTTAAACAAGATATATTTAAACTACATAAACTTGAAG
  TCTGAGAATAAGCTCAGTGGGAAAGAGGACCTTTGGGAAAAATTACAGTATTTGTGGAAGTCTACTCTG
  AATCTCCCAGAAGATCTTTTGAGGGTTCCTGATGAGTCACTCTTCTTAAATAGTGGTGGAGATTCCTTA
  AAGTCCATCCGGCTCCTCAGTGAGATTGAAAAACTTGTTGGTACATCAGTACCTGGGCTTCTGGAAATT
  ATTCTCAGCAGTTCCATTTTAGAGATTTATAATCACATCCTTCAAACAGTGGTTCCAGATGAAGATGTG
  ACATTCAGGAAGAGTTGTGCCACAAAAAGGAAACTCAGCGACATTAATCAAGAGGAAGCCAGTGGAACA
  TCTTTACATCAGAAAGCCATCATGACTTTCACTTGCCACAATGAGATTAATGCTTTTGTTGTACTGAGC
  AGAGGGAGTCAAATTTTGTCTCTGAATTCCACTAGGTTTTTAACAAAGTTAGGACATTGCTCTTCAGCC
  TGTCCTTCTGACTCAGTTTCACAGACCAACATTCAAAATTTGAAAGGCTTAAATTCTCCAGTTCTTATT
  GGGAAGTCAAAAGATCCATCCTGTGTTGCAAAAGTTTCTGAAGAGGGGAAACCTGCGATAGGGACTCAG
  AAAATGGAGTTACATGTGAGGTGGAGGTCAGACACAGGCAAATGTGTAGATGCTTCACCGCTGGTTGTA
  ATACCCACTTTTGATAAGTCATCTACAACTGTGTACATTGGTTCCCATTCTCATAGAATGAAGGCAGTT
  GACTTTTACTCTGGGAAGGTAAAATGGGAACAGATTTTGGGAGATCGAATTGAATCCTCAGCATGTGTA
  TCTAAGTGTGGAAACTTTATTGTGGTGGGCTGTTATAATGGATTAGTTTATGTTCTGAAAAGTAATAGT
  GGAGAAAAATACTGGATGTTTACTACTGAAGATGCTGTCAAAAGCTCGGCAACCATGGATCCAACCACA
  GGACTCATTTACATTGGATCTCATGACCAGCACGCATATGCTTTAGATATTTATAGAAAGAAGTGTGTT
  TGGAAGTCAAAATGTGGAGGAACTGTCTTTTCCTCTCCGTGTTTGAACCTGATTCCACATCATTTGTAT
  TTTGCTACATTGGGAGGACTTTTACTGGCTGTAAATCCTGCTACTGGGAACGTTATTTGGAAACATTCC
  TGTGGAAAACCACTCTTCTCTTCCCCACAATGTTGCTCACAGTATATTTGTATTGGCTGTGTAGATGGG
  AATTTACTCTGCTTTACTCACTTTGGAGAACAGGTTTGGCAGTTCTCTACCAGTGGACCAATCTTTTCA
  TCCCCGTGTACCTCACCATCAGAGCAAAAAATATTTTTTGGTTCCCATGATTGCTTTATCTACTGTTGT
  AACATGAAAGGTCACCTGCAGTGGAAATTTGAAACTACTTCAAGGGTCTATGCAACACCGTTTGCTTTC
  CATAACTACAATGGCAGCAATGAAATGTTGCTGGCAGCAGCATCTACTGATGGGAAAGTGTGGATCTTG
  GAATCTCAGAGTGGACAATTGCAAAGTGTTTATGAACTTCCTGGAGAAGTCTTCTCTTCTCCTGTGGTC
  CTGGAATCAATGCTCATTATTGGGTGTAGAGATAATTATGTTTATTGTCTGGATTTATTGGGTGGCAAT
  CAAAAA
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG239811
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MLNDGKEKYEKEKIKSISSEHVNEEKAEEHMDLRLKHCLAYVLHTSGTTGIPKIVRVPHKCIVPNIQHF
  RVLFDITQEDVLFLASPLTFDPSVVEIFLALSSGASLLIVPTSVKLLPSKLASVLFSHHRVTVLQATPT
  LLRRFGSQLIKSTVLSATTSLRVLALGGEAFPSLTVLRSWRGEGNKTQIFNVYGITEVSSWATIYRIPE
  KTLNSTLKCELPVQLGFPLLGTVVEVRDTNGFTIQEGSGQVFLGGRNRVCFLDDEVTVPLGTMRATGDF
  VTVKDGEIFFLGRKDSQIKRHGKRLNIELVQQVAEELQQVESCAVTWYNQEKLILFMVSKDASVKEYIF
  KELQKYLPSHAVPDELVLIDSLPFTSHGKIDVSELNKIYLNYINLKSENKLSGKEDLWEKLQYLWKSTL
  NLPEDLLRVPDESLFLNSGGDSLKSIRLLSEIEKLVGTSVPGLLEIILSSSILEIYNHILQTVVPDEDV
  TFRKSCATKRKLSDINQEEASGTSLHQKAIMTFTCHNEINAFVVLSRGSQILSLNSTRFLTKLGHCSSA
  CPSDSVSQTNIQNLKGLNSPVLIGKSKDPSCVAKVSEEGKPAIGTQKMELHVRWRSDTGKCVDASPLVV
  IPTFDKSSTTVYIGSHSHRMKAVDFYSGKVKWEQILGDRIESSACVSKCGNFIVVGCYNGLVYVLKSNS
  GEKYWMFTTEDAVKSSATMDPTTGLIYIGSHDQHAYALDIYRKKCVWKSKCGGTVFSSPCLNLIPHHLY
  FATLGGLLLAVNPATGNVIWKHSCGKPLFSSPQCCSQYICIGCVDGNLLCFTHFGEQVWQFSTSGPIFS
  SPCTSPSEQKIFFGSHDCFIYCCNMKGHLQWKFETTSRVYATPFAFHNYNGSNEMLLAAASTDGKVWIL
  ESQSGQLQSVYELPGEVFSSPVVLESMLIIGCRDNYVYCLDLLGGNQK
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001286669
• ORF Size: 2835 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001286669.1, NP_001273598.1
• RefSeq Size: 3493 bp
• RefSeq ORF: 2838 bp
• Locus ID: 132949
• Cytogenetics: 4q12
• Protein Pathways: Lysine biosynthesis, Lysine degradation, Metabolic pathways
• MW: 105.4 kDa
• Gene Summary: This gene encodes a member of the non-ribosome peptide syntesase (NRPS) enzyme family. The encoded protein contains an AMP-binding domain, PP-binding (phosphopantetheine, or pantetheine 4'phosphate-binding) domain and the Pyrrolo-quinoline quinon (PQQ) binding domain. The protein is expressed in several adult tissues. [provided by RefSeq, Apr 2016]