POLR1B (NM_001282779) Human Tagged ORF Clone

CAT#: RG239865

POLR1B (GFP-tagged) - Human polymerase (RNA) I polypeptide B, 128kDa (POLR1B), transcript variant 7

Specifications

Product Data

• Tag: TurboGFP
• Symbol: POLR1B
• Synonyms: RPA2; RPA135; Rpo1-2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG239865 representing NM_001282779.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGTGAAATCCAAGCTTTGCAACTTACGTAACCTTCCCCCACAAGCCCTCATTGAGCACCATGAGGAG
  GCAGAGGAAATGGGGGGCTATTTTATAATCAATGGCATTGAAAAAGTCATCCGAATGTTGATTATGCCT
  CGGAGAAATTTTCCCATTGCAATGATAAGACCAAAATGGAAAACCAGAGGGCCTGGTTATACTCAGTAT
  GGAGTTTCAATGCACTGTGTGAGGGAAGAACATTCCGCTGTCAATATGAACCTCCACTACTTGGAAAAT
  GGCACTGTTATGTTGAACTTTATTTACCGAAAAGAACTGTTCTTTCTTCCTTTGGGATTTGCACTTAAG
  GCACTTGTCAGCTTTTCTGATTATCAGATCTTTCAGGAGCTCATCAAAGGAAAAGAGGATGATTCTTTC
  CTTAGGAACTCTGTTTCTCAGATGTTAAGGATTGTAATGGAAGAGGGTTGTTCGACACAAAAACAGGTC
  CTTAACTACCTAGGTGAATGCTTCAGAGTAAAACTCAATGTTCCTGACTGGTACCCAAATGAGCAAGCT
  GCGGAGTTCCTGTTTAACCAGTGCATCTGTATCCACTTGAAATCCAATACTGAAAAGTTTTATATGCTT
  TGTCTCATGACGCGAAAGCTCTTTGCTTTAGCCAAAGGAGAGTGCATGGAGGACAATCCTGATAGTTTG
  GTGAACCAGGAAGTCCTCACACCGGGTCAGCTCTTCCTTATGTTCCTGAAGGAAAAACTGGAAGGTTGG
  TTAGTGTCTATTAAAATAGCTTTTGATAAGAAGGCTCAGAAGACCAGTGTTTCCATGAACACTGACAAT
  TTGATGAGGATTTTTACAATGGGCATAGACCTTACAAAACCATTTGAATACCTTTTTGCTACTGGGAAT
  CTGCGTTCTAAAACAGGTCTTGGCCTCCTACAAGATTCTGGACTTTGTGTTGTGGCTGACAAGCTGAAC
  TTCATACGCTACCTCTCCCATTTCCGCTGCGTGCACAGAGGGGCTGATTTTGCCAAGATGAGGACCACC
  ACAGTACGCAGGCTGCTGCCAGAGTCCTGGGGCTTCCTTTGTCCCGTGCATACCCCAGACGGGGAGCCC
  TGTGGCCTGATGAACCACCTAACTGCCGTATGTGAGGTTGTCACACAGTTTGTGTATACGGCATCTATT
  CCAGCTTTACTGTGCAACTTGGGGGTCACTCCCATTGATGGAGCTCCCCACCGATCATACAGTGAGTGC
  TACCCTGTCCTGCTGGACGGTGTCATGGTTGGCTGGGTGGATAAGGATCTTGCTCCAGGCATCGCAGAT
  TCTCTTCGTCATTTTAAGGTGTTGAGAGAGAAAAGAATTCCTCCCTGGATGGAAGTGGTCCTTATACCC
  ATGACAGGAAAACCAAGTCTGTACCCAGGATTGTTCCTTTTTACCACTCCTTGTAGACTGGTACGGCCT
  GTGCAGAACTTAGCATTGGGCAAAGAAGAGCTAATTGGAACTATGGAACAGATCTTCATGAATGTCGCT
  ATCTTTGAGGATGAAGTTTTTGCTGGAGTTACCACACACCAGGAACTCTTTCCACACAGCCTGCTGAGT
  GTGATTGCCAACTTCATCCCTTTCTCTGATCACAACCAGAGTCCACGGAACATGTACCAATGCCAGATG
  GGTAAGCAAACTATGGGCTTTCCACTTCTCACTTATCAAGACCGATCGGATAACAAACTGTATCGTCTT
  CAGACTCCTCAGAGTCCCTTGGTGAGACCCTCCATGTATGATTATTATGACATGGATAACTATCCAATT
  GGGACCAATGCCATCGTTGCTGTGATTTCTTACACTGGCTATGATATGGAAGATGCCATGATTGTGAAT
  AAGGCCTCTTGGGAACGAGGCTTTGCCCATGGAAGTGTCTACAAGTCTGAGTTCATAGACCTCTCTGAA
  AAAATTAAACAAGGAGATAGTAGCCTGGTGTTTGGCATCAAACCTGGTGACCCACGCGTTCTGCAGAAG
  TTAGATGACGATGGATTGCCGTTTATAGGAGCAAAACTGCAGTACGGAGATCCGTATTACAGCTACCTC
  AACCTCAACACCGGGGAAAGTTTTGTGATGTACTATAAGAGTAAAGAAAATTGTGTTGTGGATAACATC
  AAAGTGTGCAGTAATGACACTGGGAGTGGAAAATTCAAGTGTGTTTGCATCACTATGAGAGTGCCTCGG
  AACCCAACTATCGGAGATAAATTTGCCAGTCGCCATGGGCAGAAGGGCATTTTAAGCAGATTGTGGCCG
  GCTGAGGACATGCCTTTTACTGAGAGTGGGATGGTCCCAGACATTCTGTTCAATCCCCATGGTTTTCCA
  TCCCGCATGACCATTGGGATGTTAATTGAGAGTATGGCCGGGAAGTCTGCAGCTTTGCATGGTCTCTGC
  CATGATGCTACACCCTTCATCTTCTCAGAGGAGAACTCGGCCTTAGAATACTTTGGTGAGATGTTAAAG
  GCTGCTGGCTACAATTTCTATGGCACCGAGAGGTTATATAGTGGCATCAGTGGGCTAGAACTGGAAGCA
  GACATCTTCATAGGAGTGGTTTATTATCAGCGCTTACGCCATATGGTCTCAGACAAATTTCAAGTAAGG
  ACAACTGGAGCCCGAGACAGAGTCACCAACCAGCCTATTGGGGGAAGAAATGTCCAGGGTGGAATCCGT
  TTTGGGGAGATGGAACGGGATGCGCTTTTAGCTCATGGTACATCTTTTCTCCTTCATGACCGCCTCTTC
  AACTGCTCAGATCGGTCGGTAGCCCATGTGTGTGTGAAGTGTGGCAGTTTACTCTCTCCACTGTTGGAG
  AAGCCACCCCCTTCTTGGTCTGCCATGCGCAACAGAAAATACAACTGTACTCTGTGTAGTCGCAGTGAC
  ACTATCGATACTGTTTCTGTGCCTTATGTTTTTCGGTATTTTGTAGCTGAACTGGCAGCTATGAACATC
  AAAGTGAAACTGGATGTTGTT
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG239865
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MVKSKLCNLRNLPPQALIEHHEEAEEMGGYFIINGIEKVIRMLIMPRRNFPIAMIRPKWKTRGPGYTQY
  GVSMHCVREEHSAVNMNLHYLENGTVMLNFIYRKELFFLPLGFALKALVSFSDYQIFQELIKGKEDDSF
  LRNSVSQMLRIVMEEGCSTQKQVLNYLGECFRVKLNVPDWYPNEQAAEFLFNQCICIHLKSNTEKFYML
  CLMTRKLFALAKGECMEDNPDSLVNQEVLTPGQLFLMFLKEKLEGWLVSIKIAFDKKAQKTSVSMNTDN
  LMRIFTMGIDLTKPFEYLFATGNLRSKTGLGLLQDSGLCVVADKLNFIRYLSHFRCVHRGADFAKMRTT
  TVRRLLPESWGFLCPVHTPDGEPCGLMNHLTAVCEVVTQFVYTASIPALLCNLGVTPIDGAPHRSYSEC
  YPVLLDGVMVGWVDKDLAPGIADSLRHFKVLREKRIPPWMEVVLIPMTGKPSLYPGLFLFTTPCRLVRP
  VQNLALGKEELIGTMEQIFMNVAIFEDEVFAGVTTHQELFPHSLLSVIANFIPFSDHNQSPRNMYQCQM
  GKQTMGFPLLTYQDRSDNKLYRLQTPQSPLVRPSMYDYYDMDNYPIGTNAIVAVISYTGYDMEDAMIVN
  KASWERGFAHGSVYKSEFIDLSEKIKQGDSSLVFGIKPGDPRVLQKLDDDGLPFIGAKLQYGDPYYSYL
  NLNTGESFVMYYKSKENCVVDNIKVCSNDTGSGKFKCVCITMRVPRNPTIGDKFASRHGQKGILSRLWP
  AEDMPFTESGMVPDILFNPHGFPSRMTIGMLIESMAGKSAALHGLCHDATPFIFSEENSALEYFGEMLK
  AAGYNFYGTERLYSGISGLELEADIFIGVVYYQRLRHMVSDKFQVRTTGARDRVTNQPIGGRNVQGGIR
  FGEMERDALLAHGTSFLLHDRLFNCSDRSVAHVCVKCGSLLSPLLEKPPPSWSAMRNRKYNCTLCSRSD
  TIDTVSVPYVFRYFVAELAAMNIKVKLDVV
  SGPTRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLL
  SHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGF
  PEDSVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGP
  MFAFRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001282779
• ORF Size: 2988 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001282779.2
• RefSeq Size: 5356 bp
• RefSeq ORF: 2991 bp
• Locus ID: 84172
• Cytogenetics: 2q14.1
• Protein Families: Transcription Factors
• Protein Pathways: Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase
• MW: 113.3 kDa
• Gene Summary: Eukaryotic RNA polymerase I (pol I) is responsible for the transcription of ribosomal RNA (rRNA) genes and production of rRNA, the primary component of ribosomes. Pol I is a multisubunit enzyme composed of 6 to 14 polypeptides, depending on the species. Most of the mass of the pol I complex derives from the 2 largest subunits, Rpa1 and Rpa2 in yeast. POLR1B is homologous to Rpa2 (Seither and Grummt, 1996 [PubMed 8921381]). [supplied by OMIM, Mar 2008]