CARD15 (NOD2) (NM_001293557) Human Tagged ORF Clone

CAT#: RG239883

NOD2 (GFP-tagged) - Human nucleotide-binding oligomerization domain containing 2 (NOD2), transcript variant 2

Specifications

Product Data

• Tag: TurboGFP
• Symbol: NOD2
• Synonyms: ACUG; BLAU; BLAUS; CARD15; CD; CLR16.3; IBD1; NLRC2; NOD2B; PSORAS1; YAOS
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG239883 representing NM_001293557.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGTGCTCGCAGGAGGCTTTTCAGGCACAGAGGAGCCAGCTGGTCGAGCTGCTGGTCTCAGGGTCCCTG
  GAAGGCTTCGAGAGTGTCCTGGACTGGCTGCTGTCCTGGGAGGTCCTCTCCTGGGAGGACTACGAGGGC
  TTCCACCTCCTGGGCCAGCCTCTCTCCCACTTGGCCAGGCGCCTTCTGGACACCGTCTGGAATAAGGGT
  ACTTGGGCCTGTCAGAAGCTCATCGCGGCTGCCCAAGAAGCCCAGGCCGACAGCCAGTCCCCCAAGCTG
  CATGGCTGCTGGGACCCCCACTCGCTCCACCCAGCCCGAGACCTGCAGAGTCACCGGCCAGCCATTGTC
  AGGAGGCTCCACAGCCATGTGGAGAACATGCTGGACCTGGCATGGGAGCGGGGTTTCGTCAGCCAGTAT
  GAATGTGATGAAATCAGGTTGCCGATCTTCACACCGTCCCAGAGGGCAAGAAGGCTGCTTGATCTTGCC
  ACGGTGAAAGCGAATGGATTGGCTGCCTTCCTTCTACAACATGTTCAGGAATTACCAGTCCCATTGGCC
  CTGCCTTTGGAAGCTGCCACATGCAAGAAGTATATGGCCAAGCTGAGGACCACGGTGTCTGCTCAGTCT
  CGCTTCCTCAGTACCTATGATGGAGCAGAGACGCTCTGCCTGGAGGACATATACACAGAGAATGTCCTG
  GAGGTCTGGGCAGATGTGGGCATGGCTGGACCCCCGCAGAAGAGCCCAGCCACCCTGGGCCTGGAGGAG
  CTCTTCAGCACCCCTGGCCACCTCAATGACGATGCGGACACTGTGCTGGTGGTGGGTGAGGCGGGCAGT
  GGCAAGAGCACGCTCCTGCAGCGGCTGCACTTGCTGTGGGCTGCAGGGCAAGACTTCCAGGAATTTCTC
  TTTGTCTTCCCATTCAGCTGCCGGCAGCTGCAGTGCATGGCCAAACCACTCTCTGTGCGGACTCTACTC
  TTTGAGCACTGCTGTTGGCCTGATGTTGGTCAAGAAGACATCTTCCAGTTACTCCTTGACCACCCTGAC
  CGTGTCCTGTTAACCTTTGATGGCTTTGACGAGTTCAAGTTCAGGTTCACGGATCGTGAACGCCACTGC
  TCCCCGACCGACCCCACCTCTGTCCAGACCCTGCTCTTCAACCTTCTGCAGGGCAACCTGCTGAAGAAT
  GCCCGCAAGGTGGTGACCAGCCGTCCGGCCGCTGTGTCGGCGTTCCTCAGGAAGTACATCCGCACCGAG
  TTCAACCTCAAGGGCTTCTCTGAACAGGGCATCGAGCTGTACCTGAGGAAGCGCCATCATGAGCCCGGG
  GTGGCGGACCGCCTCATCCGCCTGCTCCAAGAGACCTCAGCCCTGCACGGTTTGTGCCACCTGCCTGTC
  TTCTCATGGATGGTGTCCAAATGCCACCAGGAACTGTTGCTGCAGGAGGGGGGGTCCCCAAAGACCACT
  ACAGATATGTACCTGCTGATTCTGCAGCATTTTCTGCTGCATGCCACCCCCCCAGACTCAGCTTCCCAA
  GGTCTGGGACCCAGTCTTCTTCGGGGCCGCCTCCCCACCCTCCTGCACCTGGGCAGACTGGCTCTGTGG
  GGCCTGGGCATGTGCTGCTACGTGTTCTCAGCCCAGCAGCTCCAGGCAGCACAGGTCAGCCCTGATGAC
  ATTTCTCTTGGCTTCCTGGTGCGTGCCAAAGGTGTCGTGCCAGGGAGTACGGCGCCCCTGGAATTCCTT
  CACATCACTTTCCAGTGCTTCTTTGCCGCGTTCTACCTGGCACTCAGTGCTGATGTGCCACCAGCTTTG
  CTCAGACACCTCTTCAATTGTGGCAGGCCAGGCAACTCACCAATGGCCAGGCTCCTGCCCACGATGTGC
  ATCCAGGCCTCGGAGGGAAAGGACAGCAGCGTGGCAGCTTTGCTGCAGAAGGCCGAGCCGCACAACCTT
  CAGATCACAGCAGCCTTCCTGGCAGGGCTGTTGTCCCGGGAGCACTGGGGCCTGCTGGCTGAGTGCCAG
  ACATCTGAGAAGGCCCTGCTCCGGCGCCAGGCCTGTGCCCGCTGGTGTCTGGCCCGCAGCCTCCGCAAG
  CACTTCCACTCCATCCCGCCAGCTGCACCGGGTGAGGCCAAGAGCGTGCATGCCATGCCCGGGTTCATC
  TGGCTCATCCGGAGCCTGTACGAGATGCAGGAGGAGCGGCTGGCTCGGAAGGCTGCACGTGGCCTGAAT
  GTTGGGCACCTCAAGTTGACATTTTGCAGTGTGGGCCCCACTGAGTGTGCTGCCCTGGCCTTTGTGCTG
  CAGCACCTCCGGCGGCCCGTGGCCCTGCAGCTGGACTACAACTCTGTGGGTGACATTGGCGTGGAGCAG
  CTGCTGCCTTGCCTTGGTGTCTGCAAGGCTCTGTATTTGCGCGATAACAATATCTCAGACCGAGGCATC
  TGCAAGCTCATTGAATGTGCTCTTCACTGCGAGCAATTGCAGAAGTTAGCTCTATTCAACAACAAATTG
  ACTGACGGCTGTGCACACTCCATGGCTAAGCTCCTTGCATGCAGGCAGAACTTCTTGGCATTGAGGCTG
  GGGAATAACTACATCACTGCCGCGGGAGCCCAAGTGCTGGCCGAGGGGCTCCGAGGCAACACCTCCTTG
  CAGTTCCTGGGATTCTGGGGCAACAGAGTGGGTGACGAGGGGGCCCAGGCCCTGGCTGAAGCCTTGGGT
  GATCACCAGAGCTTGAGGTGGCTCAGCCTGGTGGGGAACAACATTGGCAGTGTGGGTGCCCAAGCCTTG
  GCACTGATGCTGGCAAAGAACGTCATGCTAGAAGAACTCTGCCTGGAGGAGAACCATCTCCAGGATGAA
  GGTGTATGTTCTCTCGCAGAAGGACTGAAGAAAAATTCAAGTTTGAAAATCCTGAAGTTGTCCAATAAC
  TGCATCACCTACCTAGGGGCAGAAGCCCTCCTGCAGGCCCTTGAAAGGAATGACACCATCCTGGAAGTC
  TGGCTCCGAGGGAACACTTTCTCTCTAGAGGAGGTTGACAAGCTCGGCTGCAGGGACACCAGACTCTTG
  CTT
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG239883
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MCSQEAFQAQRSQLVELLVSGSLEGFESVLDWLLSWEVLSWEDYEGFHLLGQPLSHLARRLLDTVWNKG
  TWACQKLIAAAQEAQADSQSPKLHGCWDPHSLHPARDLQSHRPAIVRRLHSHVENMLDLAWERGFVSQY
  ECDEIRLPIFTPSQRARRLLDLATVKANGLAAFLLQHVQELPVPLALPLEAATCKKYMAKLRTTVSAQS
  RFLSTYDGAETLCLEDIYTENVLEVWADVGMAGPPQKSPATLGLEELFSTPGHLNDDADTVLVVGEAGS
  GKSTLLQRLHLLWAAGQDFQEFLFVFPFSCRQLQCMAKPLSVRTLLFEHCCWPDVGQEDIFQLLLDHPD
  RVLLTFDGFDEFKFRFTDRERHCSPTDPTSVQTLLFNLLQGNLLKNARKVVTSRPAAVSAFLRKYIRTE
  FNLKGFSEQGIELYLRKRHHEPGVADRLIRLLQETSALHGLCHLPVFSWMVSKCHQELLLQEGGSPKTT
  TDMYLLILQHFLLHATPPDSASQGLGPSLLRGRLPTLLHLGRLALWGLGMCCYVFSAQQLQAAQVSPDD
  ISLGFLVRAKGVVPGSTAPLEFLHITFQCFFAAFYLALSADVPPALLRHLFNCGRPGNSPMARLLPTMC
  IQASEGKDSSVAALLQKAEPHNLQITAAFLAGLLSREHWGLLAECQTSEKALLRRQACARWCLARSLRK
  HFHSIPPAAPGEAKSVHAMPGFIWLIRSLYEMQEERLARKAARGLNVGHLKLTFCSVGPTECAALAFVL
  QHLRRPVALQLDYNSVGDIGVEQLLPCLGVCKALYLRDNNISDRGICKLIECALHCEQLQKLALFNNKL
  TDGCAHSMAKLLACRQNFLALRLGNNYITAAGAQVLAEGLRGNTSLQFLGFWGNRVGDEGAQALAEALG
  DHQSLRWLSLVGNNIGSVGAQALALMLAKNVMLEELCLEENHLQDEGVCSLAEGLKKNSSLKILKLSNN
  CITYLGAEALLQALERNDTILEVWLRGNTFSLEEVDKLGCRDTRLLL
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001293557
• ORF Size: 3039 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001293557.2
• RefSeq Size: 4459 bp
• RefSeq ORF: 3042 bp
• Locus ID: 64127
• Cytogenetics: 16q12.1
• Protein Families: Druggable Genome
• Protein Pathways: NOD-like receptor signaling pathway
• MW: 113 kDa
• Gene Summary: This gene is a member of the Nod1/Apaf-1 family and encodes a protein with two caspase recruitment (CARD) domains and six leucine-rich repeats (LRRs). The protein is primarily expressed in the peripheral blood leukocytes. It plays a role in the immune response to intracellular bacterial lipopolysaccharides (LPS) by recognizing the muramyl dipeptide (MDP) derived from them and activating the NFKB protein. Mutations in this gene have been associated with Crohn disease and Blau syndrome. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jun 2014]