ITGA8 (NM_001291494) Human Tagged ORF Clone

CAT#: RG239915

ITGA8 (GFP-tagged) - Human integrin, alpha 8 (ITGA8), transcript variant 2

Specifications

Product Data

• Tag: TurboGFP
• Symbol: ITGA8
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG239915 representing NM_001291494.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGTCGCCCGGGGCCAGCCGCGGTCCCCGGGGAAGCCAGGCGCCGCTGATCGCGCCCCTCTGCTGCGCC
  GCGGCCGCGCTGGGGATGTTGCTGTGGTCCCCCGCCTGTCAGGCGTTCAACCTGGACGTGGAAAAGCTC
  ACAGTGTACAGCGGCCCCAAGGGCAGCTACTTCGGCTACGCCGTGGACTTCCACATACCCGACGCCCGC
  ACAGCGAGTGTCTTGGTGGGGGCGCCCAAAGCCAACACCAGCCAGCCCGATATCGTGGAAGGGGGAGCC
  GTCTATTACTGTCCTTGGCCCGCGGAGGGGTCTGCGCAGTGCAGGCAGATACCGTTTGACACCACCAAC
  AACAGAAAGATCAGAGTTAATGGAACCAAAGAACCTATCGAGTTCAAATCCAATCAGTGGTTTGGAGCA
  ACAGTGAAAGCTCACAAAGGAAAAGTTGTGGCCTGTGCTCCTTTATATCACTGGAGAACTCTTAAACCG
  ACACCAGAAAAGGACCCAGTTGGCACCTGCTATGTAGCAATTCAGAACTTCAGCGCCTATGCCGAGTTC
  TCTCCTTGCCGGAACAGCAATGCTGATCCGGAAGGCCAGGGTTACTGCCAAGCAGGATTTAGTCTGGAT
  TTTTATAAGAATGGAGACCTTATTGTGGGAGGACCTGGGAGTTTCTACTGGCAAGGACAAGTGATCACT
  GCCAGTGTTGCAGATATCATTGCAAATTACTCATTCAAGGATATCCTCAGGAAACTGGCAGGAGAAAAG
  CAGACGGAAGTGGCTCCAGCTTCCTATGATGACAGTTACCTTGAATTGGTTGCTGGAATTCCAAGAGGA
  GCACAGAATTTTGGATATGTTTCCATCATTAACTCTACGGATATGACGTTTATTCAGAATTTCACGGGA
  GAACAGATGGCATCTTATTTTGGATATACCGTTGTCGTATCAGATGTTAACAGTGATGGACTGGATGAT
  GTCCTGGTTGGGGCACCTCTCTTTATGGAACGTGAATTTGAGAGCAACCCCAGAGAAGTAGGGCAAATC
  TACCTGTATTTGCAAGTGAGCTCTCTCCTCTTCAGAGACCCCCAGATCCTCACTGGCACCGAGACGTTT
  GGGAGATTCGGTAGTGCTATGGCACACTTAGGAGACCTGAACCAAGATGGATACAATGACATTGCCATC
  GGAGTGCCTTTTGCAGGCAAGGATCAAAGAGGCAAAGTGCTCATTTATAATGGGAACAAAGATGGCTTA
  AACACCAAGCCTTCCCAAGTTCTGCAAGGAGTGTGGGCCTCACATGCTGTCCCTTCCGGATTTGGCTTT
  ACTTTAAGAGGAGATTCAGACATAGACAAGAATGATTACCCAGATTTGATTGTGGGTGCATTTGGAACA
  GGAAAAGTCGCTGTTTACAGAGCAAGACCGGTTGTGACTGTAGATGCCCAGCTTCTGCTGCACCCAATG
  ATTATCAATCTTGAAAATAAAACTTGCCAGGTTCCAGACTCTATGACATCTGCTGCCTGCTTTTCTTTA
  AGAGTATGTGCATCTGTCACAGGCCAGAGCATTGCAAACACAATAGTCTTGATGGCAGAGGTGCAATTA
  GATTCCCTGAAACAGAAAGGAGCTATTAAACGGACGCTCTTCCTTGATAACCATCAGGCTCATCGCGTC
  TTCCCTCTTGTGATAAAAAGGCAGAAATCCCACCAGTGCCAGGATTTCATCGTTTACCTTCGAGATGAA
  ACTGAATTCCGAGATAAATTATCTCCAATCAACATTAGTTTGAATTACAGTTTGGACGAATCCACCTTT
  AAAGAAGGCCTGGAAGTGAAACCAATATTGAACTACTACAGAGAAAACATTGTTAGTGAACAGGCTCAC
  ATTCTGGTGGACTGTGGAGAAGACAATCTGTGTGTTCCTGACTTGAAGCTGTCGGCTAGACCAGATAAG
  CATCAGGTAATCATTGGAGATGAAAATCACCTTATGCTCATAATAAATGCAAGAAATGAAGGGGAAGGA
  GCATATGAAGCTGAACTCTTTGTAATGATACCAGAAGAGGCAGATTATGTTGGAATCGAACGCAACAAC
  AAGGGATTTCGACCACTGAGCTGTGAGTACAAGATGGAAAATGTAACCAGGATGGTGGTGTGTGACCTT
  GGGAACCCTATGGTGTCTGGAACAAATTATTCCCTGGGCCTCCGATTTGCAGTTCCACGTCTTGAGAAA
  ACAAACATGAGCATTAACTTCGATCTCCAAATCAGAAGTTCCAACAAGGACAATCCAGACAGCAATTTT
  GTGAGCCTGCAAATCAACATCACTGCTGTAGCGCAGGTGGAAATAAGAGGAGTGTCACACCCTCCGCAG
  ATTGTTCTGCCCATTCATAACTGGGAACCAGAAGAGGAGCCCCACAAAGAGGAGGAGGTTGGACCATTG
  GTGGAACATATTTATGAGCTGCACAATATTGGACCAAGTACCATCAGTGACACCATCCTGGAGGTGGGC
  TGGCCTTTCTCTGCCCGGGATGAATTTCTTCTCTATATTTTCCATATTCAAACTCTGGGACCTCTGCAG
  TGCCAACCAAATCCTAATATCAATCCACAGGATATAAAGCCTGCTGCCTCCCCAGAGGACACCCCTGAG
  CTCAGCGCCTTTTTGCGAAACTCTACTATTCCTCATCTTGTCAGGAAGAGGGATGTACATGTGGTCGAA
  TTCCACAGACAGAGCCCTGCAAAAATACTGAATTGTACAAATATCGAGTGTTTACAAATCTCCTGTGCA
  GTGGGACGACTCGAAGGAGGAGAAAGCGCAGTCCTGAAAGTCAGGTCACGATTATGGGCCCACACCTTC
  CTCCAGAGAAAAAATGATCCCTATGCTCTTGCATCCCTGGTGTCCTTTGAAGTTAAGAAGATGCCTTAT
  ACAGATCAGCCAGCAAAACTCCCAGAAGGAAGCATAGTAATTAAGACATCAGTTATTTGGGCAACTCCG
  AATGTTTCCTTCTCAATCCCATTATGGGTAATAATACTAGCAATACTTCTTGGATTGTTGGTTCTCGCC
  ATTTTAACCTTAGCTTTATGGAAGTGTGGATTCTTTGACAGAGCCAGACCTCCTCAGGAGGACATGACC
  GACAGGGAACAGCTGACAAATGACAAGACCCCTGAGGCA
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG239915
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MSPGASRGPRGSQAPLIAPLCCAAAALGMLLWSPACQAFNLDVEKLTVYSGPKGSYFGYAVDFHIPDAR
  TASVLVGAPKANTSQPDIVEGGAVYYCPWPAEGSAQCRQIPFDTTNNRKIRVNGTKEPIEFKSNQWFGA
  TVKAHKGKVVACAPLYHWRTLKPTPEKDPVGTCYVAIQNFSAYAEFSPCRNSNADPEGQGYCQAGFSLD
  FYKNGDLIVGGPGSFYWQGQVITASVADIIANYSFKDILRKLAGEKQTEVAPASYDDSYLELVAGIPRG
  AQNFGYVSIINSTDMTFIQNFTGEQMASYFGYTVVVSDVNSDGLDDVLVGAPLFMEREFESNPREVGQI
  YLYLQVSSLLFRDPQILTGTETFGRFGSAMAHLGDLNQDGYNDIAIGVPFAGKDQRGKVLIYNGNKDGL
  NTKPSQVLQGVWASHAVPSGFGFTLRGDSDIDKNDYPDLIVGAFGTGKVAVYRARPVVTVDAQLLLHPM
  IINLENKTCQVPDSMTSAACFSLRVCASVTGQSIANTIVLMAEVQLDSLKQKGAIKRTLFLDNHQAHRV
  FPLVIKRQKSHQCQDFIVYLRDETEFRDKLSPINISLNYSLDESTFKEGLEVKPILNYYRENIVSEQAH
  ILVDCGEDNLCVPDLKLSARPDKHQVIIGDENHLMLIINARNEGEGAYEAELFVMIPEEADYVGIERNN
  KGFRPLSCEYKMENVTRMVVCDLGNPMVSGTNYSLGLRFAVPRLEKTNMSINFDLQIRSSNKDNPDSNF
  VSLQINITAVAQVEIRGVSHPPQIVLPIHNWEPEEEPHKEEEVGPLVEHIYELHNIGPSTISDTILEVG
  WPFSARDEFLLYIFHIQTLGPLQCQPNPNINPQDIKPAASPEDTPELSAFLRNSTIPHLVRKRDVHVVE
  FHRQSPAKILNCTNIECLQISCAVGRLEGGESAVLKVRSRLWAHTFLQRKNDPYALASLVSFEVKKMPY
  TDQPAKLPEGSIVIKTSVIWATPNVSFSIPLWVIILAILLGLLVLAILTLALWKCGFFDRARPPQEDMT
  DREQLTNDKTPEA
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001291494
• ORF Size: 3144 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001291494.1, NP_001278423.1
• RefSeq Size: 6937 bp
• RefSeq ORF: 3147 bp
• Locus ID: 8516
• Cytogenetics: 10p13
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Arrhythmogenic right ventricular cardiomyopathy (ARVC), Cell adhesion molecules (CAMs), Dilated cardiomyopathy, ECM-receptor interaction, Focal adhesion, Hypertrophic cardiomyopathy (HCM), Regulation of actin cytoskeleton
• MW: 116.4 kDa
• Gene Summary: Integrins are heterodimeric transmembrane receptor proteins that mediate numerous cellular processes including cell adhesion, cytoskeletal rearrangement, and activation of cell signaling pathways. Integrins are composed of alpha and beta subunits. This gene encodes the alpha 8 subunit of the heterodimeric integrin alpha8beta1 protein. The encoded protein is a single-pass type 1 membrane protein that contains multiple FG-GAP repeats. This repeat is predicted to fold into a beta propeller structure. This gene regulates the recruitment of mesenchymal cells into epithelial structures, mediates cell-cell interactions, and regulates neurite outgrowth of sensory and motor neurons. The integrin alpha8beta1 protein thus plays an important role in wound-healing and organogenesis. Mutations in this gene have been associated with renal hypodysplasia/aplasia-1 (RHDA1) and with several animal models of chronic kidney disease. Alternate splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Apr 2014]