RPB2 (POLR2B) (NM_001303268) Human Tagged ORF Clone

CAT#: RG239962

POLR2B (GFP-tagged) - Human polymerase (RNA) II (DNA directed) polypeptide B, 140kDa (POLR2B), transcript variant 2

Specifications

Product Data

• Tag: TurboGFP
• Symbol: POLR2B
• Synonyms: hRPB140; POL2RB; RPB2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG239962 representing NM_001303268.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGCTGGATTGTAATCAGCCACGATATTTGCTGAAGTTTGAACAAATTTATCTTTCCAAGCCTACCCAT
  TGGGAAAGAGATGGTGCTCCTTCACCAATGATGCCCAATGAAGCTAGATTAAGGAATCTCACGTATTCT
  GCTCCGCTTTATGTTGATATAACAAAAACAGTCATTAAAGAAGGTGAAGAACAACTTCAGACTCAGCAT
  CAGAAAACTTTTATAGGAAAAATTCCAATTATGTTGCGGTCAACTTACTGCCTTTTGAATGGCTTGACA
  GATCGTGATCTTTGTGAGTTAAATGAATGCCCTTTGGATCCTGGTGGCTATTTCATTATTAATGGATCA
  GAAAAGGTTCTGATTGCCCAAGAGAAAATGGCAACAAACACAGTTTATGTGTTTGCCAAAAAGGATTCT
  AAATATGCCTACACAGGAGAGTGTAGATCATGTCTTGAGAATTCTTCCCGACCCACCAGTACTATATGG
  GTTAGCATGCTGGCAAGAGGAGGACAGGGTGCCAAGAAGAGTGCTATTGGTCAGCGCATTGTGGCAACT
  CTACCATATATCAAGCAAGAAGTTCCCATCATTATTGTGTTCAGAGCATTAGGTTTTGTGTCCGACAGA
  GATATTTTAGAACATATTATTTATGATTTTGAAGATCCAGAGATGATGGAAATGGTTAAACCTTCTCTC
  GATGAAGCTTTTGTCATCCAAGAACAGAATGTTGCACTAAATTTCATTGGTTCAAGAGGAGCAAAGCCT
  GGTGTTACTAAAGAGAAAAGAATTAAATATGCAAAGGAAGTTTTACAAAAAGAAATGCTCCCTCATGTT
  GGTGTCAGTGATTTTTGTGAGACCAAAAAAGCCTATTTCTTGGGATACATGGTTCATAGGTTACTTCTG
  GCAGCTTTGGGTAGAAGAGAACTAGATGACAGAGATCACTATGGAAACAAGAGATTGGATCTTGCTGGG
  CCGCTGCTTGCATTCTTATTTAGAGGTATGTTTAAGAATTTGCTTAAAGAAGTGCGGATCTATGCACAG
  AAATTTATTGATCGAGGAAAGGATTTTAACTTGGAGTTGGCAATTAAAACACGGATCATATCTGATGGC
  CTAAAATACTCTTTAGCTACTGGAAACTGGGGTGATCAAAAGAAAGCTCATCAAGCCAGAGCTGGAGTA
  TCTCAGGTGTTAAACCGCCTGACTTTTGCGTCTACTCTTTCTCACCTGCGTCGTTTAAATTCTCCTATT
  GGTAGAGACGGCAAGCTAGCAAAACCAAGACAGTTGCATAATACGTTGTGGGGAATGGTGTGTCCTGCC
  GAGACCCCAGAGGGCCATGCTGTAGGACTTGTGAAGAATTTAGCCTTGATGGCGTATATTTCAGTTGGA
  TCTCAACCATCTCCAATTCTGGAATTTTTAGAAGAATGGAGTATGGAAAATTTAGAAGAAATTTCTCCT
  GCAGCTATTGCTGATGCAACCAAGATTTTTGTTAATGGCTGCTGGGTTGGAATACATAAAGATCCCGAA
  CAACTTATGAACACCCTAAGGAAATTGAGACGTCAGATGGACATCATTGTGTCTGAAGTTTCTATGATC
  AGAGATATTCGAGAGAGGGAGATTCGGATCTATACGGATGCAGGCCGTATTTGTAGACCACTTCTGATT
  GTGGAAAAACAAAAGCTACTTTTGAAGAAGAGGCATATTGACCAATTGAAAGAGAGAGAATATAACAAC
  TATAGTTGGCAGGATCTTGTGGCCAGTGGGGTAGTGGAGTATATTGATACCCTGGAAGAAGAAACAGTG
  ATGCTTGCAATGACTCCAGATGATTTACAGGAGAAAGAAGTAGCTTATTGTTCCACATATACACACTGT
  GAGATTCATCCCTCAATGATCCTTGGTGTCTGTGCATCTATTATTCCCTTTCCTGATCATAACCAGTCC
  CCTAGAAACACATACCAGTCTGCTATGGGTAAGCAGGCTATGGGAGTTTACATCACCAACTTCCATGTT
  CGCATGGACACATTGGCCCATGTTCTCTATTATCCTCAAAAGCCACTTGTGACTACACGGTCTATGGAA
  TATCTACGATTTAGAGAGCTGCCAGCAGGCATCAACTCAATTGTGGCCATTGCATCATACACTGGATAT
  AATCAGGAAGACTCTGTTATCATGAATCGTTCAGCTGTAGACCGCGGCTTCTTCAGGTCTGTTTTCTAT
  CGCTCATACAAAGAACAGGAGTCTAAAAAAGGATTTGATCAAGAAGAAGTTTTTGAGAAGCCTACACGT
  GAAACATGCCAGGGCATGAGGCATGCCATTTACGACAAGCTGGATGATGATGGTTTGATAGCTCCAGGG
  GTTCGTGTATCAGGAGATGATGTTATTATAGGCAAAACAGTCACCTTGCCTGAAAATGAAGATGAATTG
  GAGAGCACCAATAGACGCTATACCAAGAGAGACTGTAGCACTTTTCTCAGAACTAGCGAGACGGGCATT
  GTGGATCAGGTTATGGTAACTCTCAATCAGGAAGGATATAAATTTTGTAAAATAAGGGTACGCTCTGTT
  AGGATTCCACAGATTGGAGACAAATTTGCTAGTCGACATGGTCAAAAGGGTACTTGTGGTATTCAGTAT
  AGACAAGAGGATATGCCTTTCACCTGTGAAGGTATCACCCCTGATATCATCATCAATCCCCATGCCATC
  CCCTCTCGTATGACTATTGGTCACTTAATTGAATGCCTTCAAGGGAAGGTATCGGCTAACAAGGGTGAA
  ATTGGTGATGCCACTCCATTTAATGATGCTGTTAACGTGCAGAAGATTTCTAATCTTTTATCTGATTAT
  GGCTATCATCTCAGAGGAAATGAGGTCCTGTACAATGGGTTCACTGGTCGAAAAATCACATCACAAATA
  TTTATTGGCCCCACTTATTACCAGCGTTTGAAGCATATGGTGGATGATAAGATTCACTCTCGTGCTAGG
  GGACCTATTCAGATCCTCAATAGACAGCCCATGGAGGGTAGATCTCGTGATGGTGGCCTGCGTTTTGGA
  GAAATGGAACGAGATTGTCAGATTGCCCATGGAGCAGCCCAGTTTTTAAGGGAAAGATTGTTTGAGGCA
  TCAGATCCATATCAGGTTCATGTTTGCAATCTTTGTGGAATAATGGCGATTGCCAACACCAGGACCCAT
  ACATATGAATGCAGGGGCTGCCGCAATAAAACCCAGATTTCTTTGGTGCGAATGCCTTACGCATGCAAA
  CTATTGTTTCAGGAACTTATGTCTATGAGTATTGCACCGCGAATGATGAGTGTT
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG239962
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MLDCNQPRYLLKFEQIYLSKPTHWERDGAPSPMMPNEARLRNLTYSAPLYVDITKTVIKEGEEQLQTQH
  QKTFIGKIPIMLRSTYCLLNGLTDRDLCELNECPLDPGGYFIINGSEKVLIAQEKMATNTVYVFAKKDS
  KYAYTGECRSCLENSSRPTSTIWVSMLARGGQGAKKSAIGQRIVATLPYIKQEVPIIIVFRALGFVSDR
  DILEHIIYDFEDPEMMEMVKPSLDEAFVIQEQNVALNFIGSRGAKPGVTKEKRIKYAKEVLQKEMLPHV
  GVSDFCETKKAYFLGYMVHRLLLAALGRRELDDRDHYGNKRLDLAGPLLAFLFRGMFKNLLKEVRIYAQ
  KFIDRGKDFNLELAIKTRIISDGLKYSLATGNWGDQKKAHQARAGVSQVLNRLTFASTLSHLRRLNSPI
  GRDGKLAKPRQLHNTLWGMVCPAETPEGHAVGLVKNLALMAYISVGSQPSPILEFLEEWSMENLEEISP
  AAIADATKIFVNGCWVGIHKDPEQLMNTLRKLRRQMDIIVSEVSMIRDIREREIRIYTDAGRICRPLLI
  VEKQKLLLKKRHIDQLKEREYNNYSWQDLVASGVVEYIDTLEEETVMLAMTPDDLQEKEVAYCSTYTHC
  EIHPSMILGVCASIIPFPDHNQSPRNTYQSAMGKQAMGVYITNFHVRMDTLAHVLYYPQKPLVTTRSME
  YLRFRELPAGINSIVAIASYTGYNQEDSVIMNRSAVDRGFFRSVFYRSYKEQESKKGFDQEEVFEKPTR
  ETCQGMRHAIYDKLDDDGLIAPGVRVSGDDVIIGKTVTLPENEDELESTNRRYTKRDCSTFLRTSETGI
  VDQVMVTLNQEGYKFCKIRVRSVRIPQIGDKFASRHGQKGTCGIQYRQEDMPFTCEGITPDIIINPHAI
  PSRMTIGHLIECLQGKVSANKGEIGDATPFNDAVNVQKISNLLSDYGYHLRGNEVLYNGFTGRKITSQI
  FIGPTYYQRLKHMVDDKIHSRARGPIQILNRQPMEGRSRDGGLRFGEMERDCQIAHGAAQFLRERLFEA
  SDPYQVHVCNLCGIMAIANTRTHTYECRGCRNKTQISLVRMPYACKLLFQELMSMSIAPRMMSV
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001303268
• ORF Size: 3297 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001303268.1, NP_001290197.1
• RefSeq Size: 3911 bp
• RefSeq ORF: 3300 bp
• Locus ID: 5431
• Cytogenetics: 4q12
• Protein Families: Transcription Factors
• Protein Pathways: Huntington's disease, Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase
• MW: 125.6 kDa
• Gene Summary: 'This gene encodes the second largest subunit of RNA polymerase II (Pol II), a DNA-dependent RNA polymerase that catalyzes the transcription of DNA into precursors of mRNA, snRNA and microRNA. This subunit and the largest subunit form opposite sides of the center cleft of Pol II. Deletion of the flap loop region of this subunit results in a decrease in the rate of transcriptional elongation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]'