PI 3 Kinase catalytic subunit gamma (PIK3CG) (NM_001282426) Human Tagged ORF Clone

CAT#: RG239965

PIK3CG (GFP-tagged) - Human phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit gamma (PIK3CG), transcript variant 2

Specifications

Product Data

• Tag: TurboGFP
• Symbol: PIK3CG
• Synonyms: p110gamma; p120-PI3K; PI3CG; PI3K; PI3Kgamma; PIK3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG239965 representing NM_001282426.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGAGCTGGAGAACTATAAACAGCCCGTGGTGCTGAGAGAGGACAACTGCCGAAGGCGCCGGAGGATG
  AAGCCGCGCAGTGCTGCGGCCAGCCTGTCCTCCATGGAGCTCATCCCCATCGAGTTCGTGCTGCCCACC
  AGCCAGCGCAAATGCAAGAGCCCCGAAACGGCGCTGCTGCACGTGGCCGGCCACGGCAACGTGGAGCAG
  ATGAAGGCCCAGGTGTGGCTGCGAGCGCTGGAGACCAGCGTGGCGGCGGACTTCTACCACCGGCTGGGA
  CCGCATCACTTCCTCCTGCTCTATCAGAAGAAGGGGCAGTGGTACGAGATCTACGACAAGTACCAGGTG
  GTGCAGACTCTGGACTGCCTGCGCTACTGGAAGGCCACGCACCGGAGCCCGGGCCAGATCCACCTGGTG
  CAGCGGCACCCGCCCTCCGAGGAGTCCCAAGCCTTCCAGCGGCAGCTCACGGCGCTGATTGGCTATGAC
  GTCACTGACGTCAGCAACGTGCACGACGATGAGCTGGAGTTCACGCGCCGTGGCTTGGTGACCCCGCGC
  ATGGCGGAGGTGGCCAGCCGCGACCCCAAGCTCTACGCCATGCACCCGTGGGTGACGTCCAAGCCCCTC
  CCGGAGTACCTGTGGAAGAAGATTGCCAACAACTGCATCTTCATCGTCATTCACCGCAGCACCACCAGC
  CAGACCATTAAGGTCTCACCCGACGACACCCCCGGCGCCATCCTGCAGAGCTTCTTCACCAAGATGGCC
  AAGAAGAAATCTCTGATGGATATTCCCGAAAGCCAAAGCGAACAGGATTTTGTGCTGCGCGTCTGTGGC
  CGGGATGAGTACCTGGTGGGCGAAACGCCCATCAAAAACTTCCAGTGGGTGAGGCACTGCCTCAAGAAC
  GGAGAAGAGATTCACGTGGTACTGGACACGCCTCCAGACCCGGCCCTAGACGAGGTGAGGAAGGAAGAG
  TGGCCACTGGTGGATGACTGCACGGGAGTCACCGGCTACCATGAGCAGCTTACCATCCACGGCAAGGAC
  CACGAGAGTGTGTTCACCGTGTCCCTGTGGGACTGCGACCGCAAGTTCAGGGTCAAGATCAGAGGCATT
  GATATCCCCGTCCTGCCTCGGAACACCGACCTCACAGTTTTTGTAGAGGCAAACATCCAGCATGGGCAA
  CAAGTCCTTTGCCAAAGGAGAACCAGCCCCAAACCCTTCACAGAGGAGGTGCTGTGGAATGTGTGGCTT
  GAGTTCAGTATCAAAATCAAAGACTTGCCCAAAGGGGCTCTACTGAACCTCCAGATCTACTGCGGTAAA
  GCTCCAGCACTGTCCAGCAAGGCCTCTGCAGAGTCCCCCAGTTCTGAGTCCAAGGGCAAAGTTCAGCTT
  CTCTATTATGTGAACCTGCTGCTGATAGACCACCGTTTCCTCCTGCGCCGTGGAGAATACGTCCTCCAC
  ATGTGGCAGATATCTGGGAAGGGAGAAGACCAAGGAAGCTTCAATGCTGACAAACTCACGTCTGCAACT
  AACCCAGACAAGGAGAACTCAATGTCCATCTCCATTCTTCTGGACAATTACTGCCACCCGATAGCCCTG
  CCTAAGCATCAGCCCACCCCTGACCCGGAAGGGGACCGGGTTCGAGCAGAAATGCCCAACCAGCTTCGC
  AAGCAATTGGAGGCGATCATAGCCACTGATCCACTTAACCCTCTCACAGCAGAGGACAAAGAATTGCTC
  TGGCATTTTAGATACGAAAGCCTTAAGCACCCAAAAGCATATCCTAAGCTATTTAGTTCAGTGAAATGG
  GGACAGCAAGAAATTGTGGCCAAAACATACCAATTGTTGGCCAGAAGGGAAGTCTGGGATCAAAGTGCT
  TTGGATGTTGGGTTAACAATGCAGCTCCTGGACTGCAACTTCTCAGATGAAAATGTAAGAGCCATTGCA
  GTTCAGAAACTGGAGAGCTTGGAGGACGATGATGTTCTGCATTACCTTCTACAATTGGTCCAGGCTGTG
  AAATTTGAACCATACCATGATAGCGCCCTTGCCAGATTTCTGCTGAAGCGTGGTTTAAGAAACAAAAGA
  ATTGGTCACTTTTTGTTTTGGTTCTTGAGAAGTGAGATAGCCCAGTCCAGACACTATCAGCAGAGGTTC
  GCTGTGATTCTGGAAGCCTATCTGAGGGGCTGTGGCACAGCCATGCTGCACGACTTTACCCAACAAGTC
  CAAGTAATCGAGATGTTACAAAAAGTCACCCTTGATATTAAATCGCTCTCTGCTGAAAAGTATGACGTC
  AGTTCCCAAGTTATTTCACAACTTAAACAAAAGCTTGAAAACCTGCAGAATTCTCAACTCCCCGAAAGC
  TTTAGAGTTCCATATGATCCTGGACTGAAAGCAGGAGCGCTGGCAATTGAAAAATGTAAAGTAATGGCC
  TCCAAGAAAAAACCACTATGGCTTGAGTTTAAATGTGCCGATCCTACAGCCCTATCAAATGAAACAATT
  GGAATTATCTTTAAACATGGTGATGATCTGCGCCAAGACATGCTTATTTTACAGATTCTACGAATCATG
  GAGTCTATTTGGGAGACTGAATCTTTGGATCTATGCCTCCTGCCATATGGTTGCATTTCAACTGGTGAC
  AAAATAGGAATGATCGAGATTGTGAAAGACGCCACGACAATTGCCAAAATTCAGCAAAGCACAGTGGGC
  AACACGGGAGCATTTAAAGATGAAGTCCTGAATCACTGGCTCAAAGAAAAATCCCCTACTGAAGAAAAG
  TTTCAGGCAGCAGTGGAGAGATTTGTTTATTCCTGTGCAGGCTACTGTGTGGCAACCTTTGTTCTTGGA
  ATAGGCGACAGACACAATGACAATATTATGATCACCGAGACAGGAAACCTATTTCATATTGACTTCGGG
  CACATTCTTGGGAATTACAAAAGTTTCCTGGGCATTAATAAAGAGAGAGTGCCATTTGTGCTAACCCCT
  GACTTCCTCTTTGTGATGGGAACTTCTGGAAAGAAGACAAGCCCACACTTCCAGAAATTTCAGGACATC
  TGTGTTAAGGCTTATCTAGCCCTTCGTCATCACACAAACCTACTGATCATCCTGTTCTCCATGATGCTG
  ATGACAGGAATGCCCCAGTTAACAAGCAAAGAAGACATTGAATATATCCGGGATGCCCTCACAGTGGGG
  AAAAATGAGGAGGATGCTAAAAAGTATTTTCTTGATCAGATCGAAGTTTGCAGAGACAAAGGATGGACT
  GTGCAGTTTAATTGGTTTCTACATCTTGTTCTTGGCATCAAACAAGGAGAGAAACATTCAGCC
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG239965
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MELENYKQPVVLREDNCRRRRRMKPRSAAASLSSMELIPIEFVLPTSQRKCKSPETALLHVAGHGNVEQ
  MKAQVWLRALETSVAADFYHRLGPHHFLLLYQKKGQWYEIYDKYQVVQTLDCLRYWKATHRSPGQIHLV
  QRHPPSEESQAFQRQLTALIGYDVTDVSNVHDDELEFTRRGLVTPRMAEVASRDPKLYAMHPWVTSKPL
  PEYLWKKIANNCIFIVIHRSTTSQTIKVSPDDTPGAILQSFFTKMAKKKSLMDIPESQSEQDFVLRVCG
  RDEYLVGETPIKNFQWVRHCLKNGEEIHVVLDTPPDPALDEVRKEEWPLVDDCTGVTGYHEQLTIHGKD
  HESVFTVSLWDCDRKFRVKIRGIDIPVLPRNTDLTVFVEANIQHGQQVLCQRRTSPKPFTEEVLWNVWL
  EFSIKIKDLPKGALLNLQIYCGKAPALSSKASAESPSSESKGKVQLLYYVNLLLIDHRFLLRRGEYVLH
  MWQISGKGEDQGSFNADKLTSATNPDKENSMSISILLDNYCHPIALPKHQPTPDPEGDRVRAEMPNQLR
  KQLEAIIATDPLNPLTAEDKELLWHFRYESLKHPKAYPKLFSSVKWGQQEIVAKTYQLLARREVWDQSA
  LDVGLTMQLLDCNFSDENVRAIAVQKLESLEDDDVLHYLLQLVQAVKFEPYHDSALARFLLKRGLRNKR
  IGHFLFWFLRSEIAQSRHYQQRFAVILEAYLRGCGTAMLHDFTQQVQVIEMLQKVTLDIKSLSAEKYDV
  SSQVISQLKQKLENLQNSQLPESFRVPYDPGLKAGALAIEKCKVMASKKKPLWLEFKCADPTALSNETI
  GIIFKHGDDLRQDMLILQILRIMESIWETESLDLCLLPYGCISTGDKIGMIEIVKDATTIAKIQQSTVG
  NTGAFKDEVLNHWLKEKSPTEEKFQAAVERFVYSCAGYCVATFVLGIGDRHNDNIMITETGNLFHIDFG
  HILGNYKSFLGINKERVPFVLTPDFLFVMGTSGKKTSPHFQKFQDICVKAYLALRHHTNLLIILFSMML
  MTGMPQLTSKEDIEYIRDALTVGKNEEDAKKYFLDQIEVCRDKGWTVQFNWFLHLVLGIKQGEKHSA
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001282426
• ORF Size: 3306 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001282426.2
• RefSeq Size: 7069 bp
• RefSeq ORF: 3309 bp
• Locus ID: 5294
• Cytogenetics: 7q22.3
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS
• Protein Pathways: Acute myeloid leukemia, Apoptosis, B cell receptor signaling pathway, Chemokine signaling pathway, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, ErbB signaling pathway, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Focal adhesion, Glioma, Inositol phosphate metabolism, Insulin signaling pathway, Jak-STAT signaling pathway, Leukocyte transendothelial migration, Melanoma, mTOR signaling pathway, Natural killer cell mediated cytotoxicity, Neurotrophin signaling pathway, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Phosphatidylinositol signaling system, Progesterone-mediated oocyte maturation, Prostate cancer, Regulation of actin cytoskeleton, Renal cell carcinoma, Small cell lung cancer, T cell receptor signaling pathway, Toll-like receptor signaling pathway, Type II diabetes mellitus, VEGF signaling pathway
• MW: 126.9 kDa
• Gene Summary: 'Phosphoinositide 3-kinases (PI3Ks) phosphorylate inositol lipids and are involved in the immune response. The protein encoded by this gene is a class I catalytic subunit of PI3K. Like other class I catalytic subunits (p110-alpha p110-beta, and p110-delta), the encoded protein binds a p85 regulatory subunit to form PI3K. This gene is located in a commonly deleted segment of chromosome 7 previously identified in myeloid leukemias. Several transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jun 2015]'