UBN1 (NM_001288656) Human Tagged ORF Clone

CAT#: RG239972

UBN1 (GFP-tagged) - Human ubinuclein 1 (UBN1), transcript variant 3

Specifications

Product Data

• Tag: TurboGFP
• Symbol: UBN1
• Synonyms: VT; VT4
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG239972 representing NM_001288656.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGTCGGAGCCCCACAGGGTCCAGTTCACCTCTCTCCCAGGTTCCCTGAATCCTGCGTTTTTGAAGAAG
  TCCCGGAAGGAGGAGGCTGGGGCAGGAGAACAGCATCAGGACTGTGAGCCGGCTGCAGCAGCTGTTCGG
  ATTACACTCACCCTCTTTGAACCAGATCACAAACGCTGCCCAGAGTTCTTCTACCCAGAGCTGGTGAAG
  AATATCCGAGGGAAGGTAAAAGGCCTTCAGCCTGGAGATAAGAAGAAAGATCTGTCAGATCCTTTCAAT
  GACGAAGAAAAGGAAAGGCATAAAGTAGAGGCCCTTGCCCGAAAATTTGAAGAAAAATACGGTGGAAAG
  AAACGTAGAAAAGACCGAATACAGGACTTGATCGATATGGGGTATGGTTATGATGAATCCGACTCCTTC
  ATCGATAACTCTGAGGCGTATGATGAGCTTGTTCCTGCTTCTTTGACTACGAAGTATGGAGGATTTTAC
  ATTAACTCGGGAACCCTGCAGTTTAGACAAGCATCAGAGTCTGAAGATGACTTCATTAAAGAAAAGAAG
  AAAAAATCTCCAAAGAAGCGGAAGTTGAAGGAAGGTGGTGAGAAGATAAAGAAGAAGAAAAAAGATGAC
  ACTTATGACAAGGAGAAGAAATCGAAAAAGTCCAAGTTTTCCAAAGCCGGCTTCACAGCCCTCAATGCC
  AGTAAGGAGAAGAAGAAGAAGAAATATTCTGGGGCTTTAAGCGTTAAAGAGATGCTAAAGAAATTTCAG
  AAAGAGAAAGAGGCTCAGAAAAAAAGGGAGGAGGAGCATAAGCCTGTTGCGGTCCCATCAGCGGAAGCT
  CAGGGCCTGCGGGAACTGGAGGGTGCCTCTGACCCCTTGCTCTCACTCTTTGGCTCTACTTCTGACAAC
  GACTTGCTCCAGGCGGCCACTGCCATGGACTCGCTGACGGATTTGGACTTGGAGCATCTGCTCAGTGAG
  TCTCCAGAAGGAAGTCCCTTCCGAGATATGGATGATGGAAGTGATTCCCTTGGGGTGGGATTGGACCAG
  GAATTCAGGCAGCCCTCTTCTCTCCCCGAAGGCCTGCCAGCACCCCTGGAGAAGCGCGTTAAGGAGCTG
  GCTCAGGCTGCCAGAGCTGCTGAGGGGGAGAGCAGACAGAAGTTCTTCACCCAGGATATTAATGGAATC
  CTATTAGACATAGAGGCGCAGACTCGGGAGCTGAGCAGTCAGGTCCGCTCTGGGGTGTATGCCTATCTT
  GCGTCATTCCTGCCCTGCAGCAAGGATGCCCTGCTCAAGCGTGCTCGGAAACTTCACCTCTATGAACAG
  GGGGGCCGTCTGAAGGAGCCTCTCCAGAAGCTCAAGGAAGCCATTGGCAGGGCGATGCCAGAGCAGATG
  GCCAAGTACCAGGACGAATGCCAGGCACACACGCAGGCAAAGGTTGCCAAGATGCTGGAAGAGGAGAAA
  GACAAGGAGCAGAGGGACCGGATTTGTTCGGATGAGGAAGAAGATGAAGAAAAAGGGGGCAGGAGGATA
  ATGGGACCTCGGAAGAAGTTCCAATGGAATGATGAGATCAGGGAGCTACTGTGCCAGGTGGTGAAGATC
  AAACTGGAGAGCCAGGACCTGGAGAGGAACAACAAAGCCCAGGCTTGGGAGGACTGTGTGAAGGGCTTT
  CTGGATGCGGAAGTCAAGCCCCTCTGGCCCAAAGGCTGGATGCAGGCCAGAACTCTGTTTAAGGAGAGC
  AGACGAGGCCATGGGCACCTGACTTCAATCCTGGCCAAGAAGAAAGTTATGGCCCCTTCTAAAATCAAG
  GTGAAGGAATCGTCTACGAAGCCTGATAAAAAGGTTTCTGTCCCATCAGGACAGATTGGTGGCCCCATT
  GCTTTGCCCTCAGATCACCAAACAGGAGGCCTGAGTATTGGGGCCTCGAGCAGGGAGCTCCCATCCCAG
  GCATCGGGCGGCCTTGCTAACCCTCCTCCTGTCAACCTGGAGGACTCATTGGATGAAGACTTGATCCGC
  AATCCAGCCTCCTCGGTGGAAGCCGTGTCCAAGGAATTGGCTGCATTGAATAGCAGAGCAGCTGGGAAC
  TCTGAATTCACACTGCCTGCACCCTCAAAAGCACCTGCAGAAAAAGTTGGAGGCGTTTTATGTACAGAA
  GAAAAAAGGAACTTTGCGAAGCCTAGTCCTTCTGCTCCACCACCAGCTAGCTCTCTGCAGTCACCCCTC
  AATTTTCTGGCAGAACAGGCTCTGGCACTGGGGCAGTCCTCTCAGGAGAAAAAACCAGAGAGTTCTGGC
  TACAAAGAGCTGTCCTGCCAGGCTCCCCTCAATAAGGGCCTGCCAGAAGTACATCAGTCCAAAGCTAAG
  CACCACAGCTTGCCACGGACGTCTCACGGGCCCCAAGTGGCAGTTCCTGTGCCTGGCCCCCAGGTCAAA
  GTCTTTCATGCCGGCACTCAGCAGCAGAAAAACTTCACGCCCCCATCTCCATTTGCCAATAAGCTCCAA
  GGCCCAAAGGCTTCTCCCACACAGTGTCATCGTTCCCTCCTGCAGTTAGTGAAGACAGCGGCCAAAGGC
  CAGGGCTTCCATCCCTCTGCACCAGCCACCTCAGGAGGCCTGTCAGCCTCCAGCAGCAGCTCTCACAAG
  ACCCCAGCCTCGTCCTCTTCTGCCCTGAGCCATCCAGCAAAGCCACATTCAGTCAGCTCTGCAGGCTCA
  TCTTACAAGAATAATCCCTTTGCCAGCTCAATCTCCAAACATGGGGTTTCTTCTGGCAGCTCTTCCTCG
  GGAGGAACACCAGTCCAGAGTTCTGTTTCTGGGAGCCTGGTCCCTGGCATACAGCCTCCCTCCGTGGGA
  CAGGCCACCAGCCGACCCGTCCCAAGTTCAGCAGGGAAAAAAATGCCTGTTTCCCAGAAGTTGACTCTG
  GTAGCCCCTCCAGGCGGTCCAAACGGAGATTCCAGTGGTGGGACCCAGGGAGTGGCAAAGTTGCTGACC
  TCGCCGTCCCTAAAGCCCTCTGCAGTTAGTAGTGTGACATCGTCTACCTCCTTGTCAAAAGGAGCGAGT
  GGGACTGTGCTGCTGGCCGGCTCCTCTTTGATGGCTTCACCCTACAAATCCAGCAGCCCAAAGCTGTCT
  GGGGCCATGAGCTCGAACTCCTTGGGAATTATAACCCCTGTCCCTATTCCTGTCCATGTGCTCTCCTTC
  AGCGCTGACTCCTCTGCCAAAGCAGGGGTCTCCAAGGATGCCATCGTCACAGGCCCTGCCCCCGGGTCC
  TTCCACCATGGCCTTGGCCACAGTGCTTCTCAGCTTCACGGGAAAGGGCCTGCTGTACCACGGAAATTG
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG239972
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MSEPHRVQFTSLPGSLNPAFLKKSRKEEAGAGEQHQDCEPAAAAVRITLTLFEPDHKRCPEFFYPELVK
  NIRGKVKGLQPGDKKKDLSDPFNDEEKERHKVEALARKFEEKYGGKKRRKDRIQDLIDMGYGYDESDSF
  IDNSEAYDELVPASLTTKYGGFYINSGTLQFRQASESEDDFIKEKKKKSPKKRKLKEGGEKIKKKKKDD
  TYDKEKKSKKSKFSKAGFTALNASKEKKKKKYSGALSVKEMLKKFQKEKEAQKKREEEHKPVAVPSAEA
  QGLRELEGASDPLLSLFGSTSDNDLLQAATAMDSLTDLDLEHLLSESPEGSPFRDMDDGSDSLGVGLDQ
  EFRQPSSLPEGLPAPLEKRVKELAQAARAAEGESRQKFFTQDINGILLDIEAQTRELSSQVRSGVYAYL
  ASFLPCSKDALLKRARKLHLYEQGGRLKEPLQKLKEAIGRAMPEQMAKYQDECQAHTQAKVAKMLEEEK
  DKEQRDRICSDEEEDEEKGGRRIMGPRKKFQWNDEIRELLCQVVKIKLESQDLERNNKAQAWEDCVKGF
  LDAEVKPLWPKGWMQARTLFKESRRGHGHLTSILAKKKVMAPSKIKVKESSTKPDKKVSVPSGQIGGPI
  ALPSDHQTGGLSIGASSRELPSQASGGLANPPPVNLEDSLDEDLIRNPASSVEAVSKELAALNSRAAGN
  SEFTLPAPSKAPAEKVGGVLCTEEKRNFAKPSPSAPPPASSLQSPLNFLAEQALALGQSSQEKKPESSG
  YKELSCQAPLNKGLPEVHQSKAKHHSLPRTSHGPQVAVPVPGPQVKVFHAGTQQQKNFTPPSPFANKLQ
  GPKASPTQCHRSLLQLVKTAAKGQGFHPSAPATSGGLSASSSSSHKTPASSSSALSHPAKPHSVSSAGS
  SYKNNPFASSISKHGVSSGSSSSGGTPVQSSVSGSLVPGIQPPSVGQATSRPVPSSAGKKMPVSQKLTL
  VAPPGGPNGDSSGGTQGVAKLLTSPSLKPSAVSSVTSSTSLSKGASGTVLLAGSSLMASPYKSSSPKLS
  GAMSSNSLGIITPVPIPVHVLSFSADSSAKAGVSKDAIVTGPAPGSFHHGLGHSASQLHGKGPAVPRKL
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001288656
• ORF Size: 3312 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001288656.1, NP_001275585.1
• RefSeq Size: 5652 bp
• RefSeq ORF: 3315 bp
• Locus ID: 29855
• Cytogenetics: 16p13.3
• MW: 119 kDa
• Gene Summary: Cellular senescence is a hallmark of tumor suppression and tissue aging. Senescent cells contain domains of heterochromatin, called senescence-associated heterochromatin foci (SAHF), that repress proliferation-promoting genes. The protein encoded by this gene binds to proliferation-promoting genes and is required for SAHF formation, enhancing methylation of histone H3. [provided by RefSeq, Oct 2016]