PRKCBP1 (ZMYND8) (NM_001281776) Human Tagged ORF Clone

CAT#: RG239976

ZMYND8 (GFP-tagged) - Human zinc finger, MYND-type containing 8 (ZMYND8), transcript variant 10

Specifications

Product Data

• Tag: TurboGFP
• Symbol: ZMYND8
• Synonyms: PRKCBP1; PRO2893; RACK7
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG239976 representing NM_001281776.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGCATCCACAGAGCTTGGCTGAAGAGGAAATAAAAACAGAACAGGAGGTGGTAGAGGGCATGGATATC
  TCTACTCGCTCCAAAGATCCTGGCTCTGCAGAGAGAACAGCCCAGAAAAGAAAGTTCCCCAGCCCTCCA
  CATTCTTCCAATGGCCACTCGCCGCAGGACACATCAACAAGCCCCATTAAAAAGAAAAAGAAACCTGGC
  TTACTGAACAGTAACAATAAGGAGCAGTCAGAACTAAGACATGGTCCGTTTTACTATATGAAGCAGCCA
  CTCACCACAGACCCTGTTGATGTTGTACCGCAGGATGGACGGAATGATTTCTACTGCTGGGTTTGTCAC
  CGGGAAGGCCAAGTCCTTTGCTGTGAGCTCTGTCCCCGGGTTTATCACGCTAAGTGTCTGAGACTGACA
  TCGGAACCAGAGGGGGACTGGTTTTGTCCTGAATGTGAGAAAATTACAGTAGCAGAATGCATCGAGACC
  CAGAGTAAAGCCATGACAATGCTCACCATTGAACAGTTATCCTACCTGCTCAAGTTTGCCATTCAGAAA
  ATGAAACAGCCAGGGACAGATGCATTCCAGAAGCCCGTTCCATTGGAACAGCACCCTGACTATGCGGAA
  TACATCTTCCATCCAATGGACCTTTGTACATTGGAAAAGAATGCGAAAAAGAAAATGTATGGCTGCACA
  GAAGCCTTCCTGGCTGATGCAAAGTGGATTTTGCACAACTGCATCATTTATAATGGGGGAAATCACAAA
  TTGACGCAAATAGCGAAAGTAGTCATCAAAATCTGTGAACATGAGATGAATGAAATCGAAGTATGTCCA
  GAATGTTATCTAGCTGCTTGCCAAAAACGAGATAACTGGTTTTGTGAGCCTTGTAGCAATCCACATCCT
  TTGGTCTGGGCCAAACTGAAGGGGTTTCCATTCTGGCCTGCAAAAGCTCTAAGGGATAAAGACGGGCAG
  GTCGATGCCCGATTCTTTGGACAACATGACAGGGCCTGGGTTCCAATAAATAATTGCTACCTCATGTCT
  AAAGAAATTCCTTTTTCTGTGAAAAAGACTAAGAGCATCTTCAACAGTGCCATGCAAGAGATGGAGGTT
  TACGTGGAGAACATCCGCAGGAAGTTTGGGGTTTTTAATTACTCTCCATTTAGGACACCCTACACACCC
  AACAGCCAGTATCAAATGCTGCTCGATCCCACCAACCCCAGCGCCGGCACTGCCAAGATAGACAAGCAG
  GAGAAGGTCAAGCTCAACTTTGACATGACGGCATCCCCCAAGATCCTGATGAGCAAGCCTGTGCTGAGT
  GGGGGCACAGGCCGCCGGATTTCCTTGTCGGATATGCCGCGCTCCCCCATGAGCACAAACTCTTCTGTG
  CACACGGGCTCCGACGTGGAGCAGGATGCTGAGAAGAAGGCCACGTCGAGCCACTTCAGTGCGAGCGAG
  GAGTCCATGGACTTCCTGGATAAGAGCACAGCTTCACCAGCCTCCACCAAGACGGGACAAGCAGGGAGT
  TTATCCGGCAGCCCAAAGCCCTTCTCTCCTCAACTGTCAGCTCCTATCACGACGAAAACGGACAAAACC
  TCCACCACCGGCAGCATCCTGAATCTTAACCTGGATCGAAGCAAAGCTGAGATGGATTTGAAGGAGCTG
  AGCGAGTCGGTCCAGCAACAGTCCACCCCTGTTCCTCTCATCTCTCCCAAGCGCCAGATTCGTAGCAGG
  TTCCAGCTGAATCTTGACAAGACCATAGAGAGTTGCAAAGCACAATTAGGCATAAATGAAATCTCGGAA
  GATGTCTATACGGCCGTAGAGCACAGCGATTCGGAGGATTCTGAGAAGTCAGATAGTAGCGATAGTGAG
  TATATCAGTGATGATGAGCAGAAGTCTAAGAACGAGCCAGAAGACACAGAGGACAAAGAAGGTTGTCAG
  ATGGACAAAGAGCCATCTGCTGTTAAAAAAAAGCCCAAGCCTACAAACCCAGTGGAGATTAAAGAGGAG
  CTGAAAAGCACGTCACCAGCCAGCGAGAAGGCAGACCCTGGAGCAGTCAAGGACAAGGCCAGCCCTGAG
  CCTGAGAAGGACTTTTCCGAAAAGGCAAAACCTTCACCTCACCCCATAAAGGATAAACTGAAGGGAAAA
  GATGAGACGGATTCCCCAACAGTCCATTTGGGCCTGGACTCTGATTCAGAGAGCGAACTTGTCATAGAT
  TTAGGAGAAGACCATTCTGGGCGGGAGGGTCGAAAAAATAAGAAGGAACCCAAAGAACCATCTCCCAAA
  CAGGATGCTGTCCAGCAGAAGGAGATCACACAGAGCCCATCCACGTCCACCATCACCCTGGTGACCAGC
  ACACAGTCATCGCCCCTGGTCACCAGCTCGGGGTCCATGAGCACCCTTGTGTCCTCAGTCAACGCTGAC
  CTGCCCATCGCCACTGCCTCAGCTGATGTCGCCGCTGATATTGCCAAGTACACTAGCAAAATGATGGAT
  GCAATAAAAGGAACAATGACAGAAATATACAACGATCTTTCTAAAAACACTACTGGAAGCACAATAGCT
  GAGATTCGCAGGCTGAGGATCGAGATAGAGAAGCTCCAGTGGCTGCACCAGCAAGAGCTCTCCGAAATG
  AAACACAACTTAGAGCTGACCATGGCGGAGATGCGGCAGAGCCTGGAGCAGGAGCGGGACCGGCTCATC
  GCCGAGGTGAAGAAGCAGCTGGAGTTGGAGAAGCAGCAGGCGGTGGATGAGACCAAGAAGAAGCAGTGG
  TGCGCCAACTGCAAGAAGGAGGCCATCTTTTACTGCTGTTGGAACACCAGCTACTGTGACTACCCCTGC
  CAGCAAGCCCACTGGCCTGAGCACATGAAGTCCTGCACCCAGTCAGCTACTGCTCCTCAGCAGGAAGCG
  GATGCTGAGGTGAACACAGAAACACTAAATAAGTCCTCCCAGGGGAGCTCCTCGAGCACACAATCAGCA
  CCTTCAGAAACGGCCAGCGCCTCCAAAGAGAAGGAGACGTCAGCTGAGAAAAGCAAGGAGAGTGGCTCG
  ACCCTTGACCTTTCTGGCTCCAGAGAGACGCCCTCCTCCATTCTCTTAGGCTCCAACCAAGGCTCTGTT
  AGCAAAAGGTGTGACAAGCAACCTGCCTATGCCCCAACCACCACAGACCACCAGCCGCACCCCAACTAC
  CCCGCCCAGAAGTACCATTCCCGGAGTAATAAATCCAGTTGGAGCAGCAGTGATGAGAAGAGGGGATCG
  ACACGTTCCGATCACAACACCAGTACCAGCACGAAGAGCCTCCTCCCGAAAGAGTCTCGGCTGGACACC
  TTCTGGGAC
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG239976
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MHPQSLAEEEIKTEQEVVEGMDISTRSKDPGSAERTAQKRKFPSPPHSSNGHSPQDTSTSPIKKKKKPG
  LLNSNNKEQSELRHGPFYYMKQPLTTDPVDVVPQDGRNDFYCWVCHREGQVLCCELCPRVYHAKCLRLT
  SEPEGDWFCPECEKITVAECIETQSKAMTMLTIEQLSYLLKFAIQKMKQPGTDAFQKPVPLEQHPDYAE
  YIFHPMDLCTLEKNAKKKMYGCTEAFLADAKWILHNCIIYNGGNHKLTQIAKVVIKICEHEMNEIEVCP
  ECYLAACQKRDNWFCEPCSNPHPLVWAKLKGFPFWPAKALRDKDGQVDARFFGQHDRAWVPINNCYLMS
  KEIPFSVKKTKSIFNSAMQEMEVYVENIRRKFGVFNYSPFRTPYTPNSQYQMLLDPTNPSAGTAKIDKQ
  EKVKLNFDMTASPKILMSKPVLSGGTGRRISLSDMPRSPMSTNSSVHTGSDVEQDAEKKATSSHFSASE
  ESMDFLDKSTASPASTKTGQAGSLSGSPKPFSPQLSAPITTKTDKTSTTGSILNLNLDRSKAEMDLKEL
  SESVQQQSTPVPLISPKRQIRSRFQLNLDKTIESCKAQLGINEISEDVYTAVEHSDSEDSEKSDSSDSE
  YISDDEQKSKNEPEDTEDKEGCQMDKEPSAVKKKPKPTNPVEIKEELKSTSPASEKADPGAVKDKASPE
  PEKDFSEKAKPSPHPIKDKLKGKDETDSPTVHLGLDSDSESELVIDLGEDHSGREGRKNKKEPKEPSPK
  QDAVQQKEITQSPSTSTITLVTSTQSSPLVTSSGSMSTLVSSVNADLPIATASADVAADIAKYTSKMMD
  AIKGTMTEIYNDLSKNTTGSTIAEIRRLRIEIEKLQWLHQQELSEMKHNLELTMAEMRQSLEQERDRLI
  AEVKKQLELEKQQAVDETKKKQWCANCKKEAIFYCCWNTSYCDYPCQQAHWPEHMKSCTQSATAPQQEA
  DAEVNTETLNKSSQGSSSSTQSAPSETASASKEKETSAEKSKESGSTLDLSGSRETPSSILLGSNQGSV
  SKRCDKQPAYAPTTTDHQPHPNYPAQKYHSRSNKSSWSSSDEKRGSTRSDHNTSTSTKSLLPKESRLDT
  FWD
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001281776
• ORF Size: 3321 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001281776.3
• RefSeq Size: 5090 bp
• RefSeq ORF: 3324 bp
• Locus ID: 23613
• Cytogenetics: 20q13.12
• Protein Families: Druggable Genome, Transcription Factors
• MW: 124 kDa
• Gene Summary: The protein encoded by this gene is a receptor for activated C-kinase (RACK) protein. The encoded protein has been shown to bind in vitro to activated protein kinase C beta I. In addition, this protein is a cutaneous T-cell lymphoma-associated antigen. Finally, the protein contains a bromodomain and two zinc fingers, and is thought to be a transcriptional regulator. Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]