AIP1 (MAGI2) (NM_001301128) Human Tagged ORF Clone

CAT#: RG240148

MAGI2 (GFP-tagged) - Human membrane associated guanylate kinase, WW and PDZ domain containing 2 (MAGI2), transcript variant 2

Specifications

Product Data

• Tag: TurboGFP
• Symbol: MAGI2
• Synonyms: ACVRIP1; AIP-1; AIP1; ARIP1; MAGI-2; NPHS15; SSCAM
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG240148 representing NM_001301128.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGTCCAAAAGCTTGAAAAAGAAAAGCCACTGGACTAGCAAAGTCCATGAGAGTGTCATTGGCAGGAAC
  CCGGAGGGCCAGCTGGGCTTTGAACTGAAGGGGGGCGCCGAGAATGGACAGTTCCCCTACCTGGGGGAG
  GTGAAGCCCGGCAAGGTGGCCTATGAGAGCGGCAGCAAATTGGTGTCGGAGGAGCTGCTGCTGGAGGTG
  AACGAGACCCCCGTGGCGGGGCTCACCATCAGGGACGTGCTGGCCGTGATCAAACACTGCAAGGACCCC
  CTCCGGCTCAAGTGTGTCAAGCAAGGAGGAATTGTTGATAAAGACCTTCGTCACTACCTCAACTTACGA
  TTTCAAAAGGGTTCTGTGGACCATGAGCTTCAGCAAATCATTCGTGACAACCTCTACCTCCGCACGGTG
  CCATGCACCACAAGGCCACATAAGGAGGGTGAGGTCCCTGGAGTGGATTATATTTTCATCACTGTTGAA
  GATTTTATGGAATTGGAGAAAAGTGGTGCTCTCCTAGAAAGTGGGACTTATGAAGACAATTACTACGGT
  ACCCCAAAGCCGCCAGCAGAACCAGCACCATTATTGTTAAATGTAACAGACCAGATACTTCCAGGAGCC
  ACTCCAAGTGCTGAAGGAAAACGGAAGAGGAATAAATCAGTGAGCAACATGGAGAAAGCCAGTATAGAG
  CCTCCTGAGGAGGAAGAGGAAGAGAGGCCTGTGGTCAATGGAAATGGAGTAGTAGTAACACCAGAATCC
  AGTGAACATGAAGACAAAAGTGCAGGTGCCTCAGGGGAGATGCCCTCCCAGCCTTATCCTGCACCAGTG
  TACAGTCAGCCTGAGGAGCTGAAGGAGCAGATGGATGACACAAAGCCAACTAAACCTGAAGACAATGAG
  GAACCAGACCCATTGCCTGATAACTGGGAAATGGCCTATACAGAGAAGGGCGAAGTCTACTTCATTGAC
  CATAACACAAAGACAACATCATGGCTGGATCCACGACTTGCGAAAAAGGCTAAACCTCCAGAAGAGTGC
  AAAGAAAATGAGCTTCCATATGGCTGGGAAAAAATCGATGATCCCATTTATGGCACTTATTATGTTGAC
  CACATAAATAGAAGAACACAGTTTGAAAATCCTGTCCTGGAAGCAAAAAGGAAGCTACAGCAACATAAC
  ATGCCCCACACAGAACTTGGAACAAAGCCCCTGCAGGCCCCAGGTTTCCGAGAAAAACCACTCTTCACC
  CGGGATGCATCCCAGTTGAAGGGAACATTCCTCAGCACCACCCTAAAAAAGAGCAACATGGGCTTTGGA
  TTTACCATCATTGGTGGAGACGAGCCTGATGAGTTTCTGCAGGTGAAAAGTGTGATTCCGGATGGGCCT
  GCAGCACAGGATGGAAAAATGGAAACAGGTGATGTCATTGTCTATATTAATGAAGTTTGTGTCCTTGGA
  CACACTCATGCAGATGTTGTCAAACTTTTCCAGTCTGTTCCTATTGGTCAGAGTGTCAACCTGGTGTTG
  TGTCGTGGCTACCCTTTGCCCTTTGATCCTGAAGACCCTGCTAACAGCATGGTGCCACCCCTTGCAATA
  ATGGAGAGGCCACCTCCAGTGATGGTCAATGGAAGACACAACTATGAAACATATTTGGAGTACATTTCT
  CGGACCTCACAGTCAGTTCCAGATATAACAGATCGGCCGCCTCATTCTCTGCACTCCATGCCAACTGAT
  GGTCAGCTAGACGGCACGTATCCACCGCCCGTCCATGATGACAATGTGTCTATGGCTTCATCTGGGGCC
  ACCCAAGCTGAACTTATGACCTTAACCATTGTGAAAGGTGCCCAGGGCTTCGGCTTCACTATTGCCGAC
  AGTCCTACAGGACAGCGGGTGAAACAAATACTTGACATTCAGGGATGCCCTGGCCTGTGTGAAGGCGAC
  CTCATTGTTGAGATCAACCAGCAGAATGTACAGAACCTGAGCCATACAGAAGTAGTGGATATACTTAAG
  GACTGTCCCATTGGAAGTGAAACTTCTTTGATTATCCATCGAGGAGGTTTCTTTTCTCCATGGAAAACT
  CCAAAGCCTATAATGGACCGATGGGAGAATCAAGGCAGTCCTCAAACGAGTTTATCTGCTCCGGCCATA
  CCGCAGAACCTGCCCTTCCCACCTGCCCTTCACAGGAGCTCCTTTCCTGACTCAACAGAGGCCTTTGAC
  CCACGGAAGCCTGATCCATATGAGCTCTACGAGAAATCTAGGGCCATTTATGAAAGTAGGCGTCCAGAT
  TATAAGGAATTGGATGTTCATCTTCGGAGGATGGAGTCTGGATTTGGCTTCAGAATCCTCGGGGGAGAT
  GAGCCTGGACAGCCTATTTTGATTGGAGCTGTCATTGCCATGGGCTCAGCCGACAGAGATGGCCGCCTT
  CACCCAGGAGATGAGCTTGTGTATGTTGATGGGATTCCAGTAGCCGGCAAAACCCACCGCTATGTCATC
  GACCTCATGCACCACGCAGCCCGCAATGGGCAGGTCAACCTCACTGTGAGAAGAAAGGTGCTATGTGGA
  GGGGAGCCCTGCCCAGAGAACGGGAGAAGTCCAGGCTCTGTATCCACCCACCACAGCTCTCCACGCAGT
  GACTACGCAACCTACACCAACAGCAACCACGCTGCCCCCAGTAGCAATGCCTCTCCCCCTGAAGGCTTC
  GCCTCCCACAGCCTGCAGACCAGTGATGTGGTCATTCACCGCAAAGAGAATGAGGGCTTCGGCTTTGTC
  ATCATCAGCTCCCTGAACAGGCCTGAGTCTGGATCCACTATAACTGTGCCCCATAAAATCGGACGCATC
  ATTGATGGGAGTCCTGCAGATCGCTGTGCAAAACTAAAAGTGGGAGACCGGATCCTAGCAGTGAATGGC
  CAGTCTATCATCAACATGCCTCACGCTGACATCGTGAAGCTCATCAAGGATGCAGGTCTTAGTGTCACC
  CTTCGCATCATTCCTCAGGAGGAGCTCAACAGCCCCACCTCGGCACCCAGCTCAGAGAAGCAGAGTCCC
  ATGGCGCAGCAGAGTCCCCTGGCACAGCAGAGTCCCCTGGCCCAGCCAAGCCCAGCCACCCCCAACAGC
  CCCATCGCCCAGCCAGCACCACCTCAACCACTTCAGCTGCAAGGACACGAAAATAGTTACAGGTCGGAA
  GTGAAAGCAAGGCAAGATGTGAAACCAGACATCCGACAGCCTCCATTCACAGACTACAGGCAGCCCCCG
  CTGGATTACAGGCAACCCCCAGGAGGGGACTACCAGCAGCCCCCACCCTTGGACTACAGGCAGCCTCCC
  CTACTGGACTACAGGCAGCACTCCCCCGACACCAGGCAGTACCCTCTGTCGGACTACAGACAACCCCAG
  GATTTTGATTATTTCACTGTGGACATGGAGAAAGGAGCCAAAGGATTTGGATTCAGCATTCGTGGAGGA
  AGGGAATACAAAATGGATTTGTATGTGTTGAGACTGGCAGAAGATGGACCAGCAATAAGGAATGGGAGG
  ATGAGGGTAGGAGATCAAATCATTGAAATCAATGGGGAAAGCACAAGGGACATGACACATGCCAGAGCA
  ATAGAACTCATCAAATCTGGAGGAAGACGAGTGAGGCTGCTGCTCAAGAGAGGCACGGGACAGGTCCCA
  GAATATGACGAACCCGCCCCCTGGAGTTCTCCCGCTGCCGCCGCCCCAGGTCTGCCGGAAGTAGGCGTC
  TCCCTGGACGACGGCCTCGCTCCATTCTCTCCATCACATCCAGCCCCACCCTCCGACCCTTCCCACCAG
  ATAAGCCCAGGCCCAACTTGGGATATCAAACGGGAACACGACGTTAGGAAACCAAAGGAGCTTTCAGCC
  TGCGGCCAGAAGAAGCAGCGCCTCGGGGAGCAGAGGGAGCGCTCGGCGAGTCCGCAGAGGGCCGCGCGG
  CCGAGGCTCGAGGAGGCGCCCGGCGGCCAGGGGCGGCCCGAGGCCGGCAGGCCCGCCTCGGAGGCCAGG
  GCGCCCGGGCTCGCGGCGGCAGACGCGGCGGACGCGGCGCGGGCGGGCGGGAAGGAGGCGCCCCGTGCG
  GCGGCGGGCTCCGAGCTCTGCCGGCGCGAAGGCCCGGGGGCTGCGCCGGCGTTTGCCGGCCCGGGCGGC
  GGCGGCAGCGGCGCGCTGGAGGCCGAGGGCAGGGCGGGTGCGCGCGCGGGTCCCCGGCCGGGCCCGCGA
  CCCCCGGGGGGCGCCCCGGCGCGCAAGGCCGCCGTCGCGCCGGGGCCCTGGAAGGTGCCGGGTTCTGAC
  AAGCTGCCCAGCGTCCTCAAACCCGGCGCCTCGGCCGCCAGCAGA
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG240148
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MSKSLKKKSHWTSKVHESVIGRNPEGQLGFELKGGAENGQFPYLGEVKPGKVAYESGSKLVSEELLLEV
  NETPVAGLTIRDVLAVIKHCKDPLRLKCVKQGGIVDKDLRHYLNLRFQKGSVDHELQQIIRDNLYLRTV
  PCTTRPHKEGEVPGVDYIFITVEDFMELEKSGALLESGTYEDNYYGTPKPPAEPAPLLLNVTDQILPGA
  TPSAEGKRKRNKSVSNMEKASIEPPEEEEEERPVVNGNGVVVTPESSEHEDKSAGASGEMPSQPYPAPV
  YSQPEELKEQMDDTKPTKPEDNEEPDPLPDNWEMAYTEKGEVYFIDHNTKTTSWLDPRLAKKAKPPEEC
  KENELPYGWEKIDDPIYGTYYVDHINRRTQFENPVLEAKRKLQQHNMPHTELGTKPLQAPGFREKPLFT
  RDASQLKGTFLSTTLKKSNMGFGFTIIGGDEPDEFLQVKSVIPDGPAAQDGKMETGDVIVYINEVCVLG
  HTHADVVKLFQSVPIGQSVNLVLCRGYPLPFDPEDPANSMVPPLAIMERPPPVMVNGRHNYETYLEYIS
  RTSQSVPDITDRPPHSLHSMPTDGQLDGTYPPPVHDDNVSMASSGATQAELMTLTIVKGAQGFGFTIAD
  SPTGQRVKQILDIQGCPGLCEGDLIVEINQQNVQNLSHTEVVDILKDCPIGSETSLIIHRGGFFSPWKT
  PKPIMDRWENQGSPQTSLSAPAIPQNLPFPPALHRSSFPDSTEAFDPRKPDPYELYEKSRAIYESRRPD
  YKELDVHLRRMESGFGFRILGGDEPGQPILIGAVIAMGSADRDGRLHPGDELVYVDGIPVAGKTHRYVI
  DLMHHAARNGQVNLTVRRKVLCGGEPCPENGRSPGSVSTHHSSPRSDYATYTNSNHAAPSSNASPPEGF
  ASHSLQTSDVVIHRKENEGFGFVIISSLNRPESGSTITVPHKIGRIIDGSPADRCAKLKVGDRILAVNG
  QSIINMPHADIVKLIKDAGLSVTLRIIPQEELNSPTSAPSSEKQSPMAQQSPLAQQSPLAQPSPATPNS
  PIAQPAPPQPLQLQGHENSYRSEVKARQDVKPDIRQPPFTDYRQPPLDYRQPPGGDYQQPPPLDYRQPP
  LLDYRQHSPDTRQYPLSDYRQPQDFDYFTVDMEKGAKGFGFSIRGGREYKMDLYVLRLAEDGPAIRNGR
  MRVGDQIIEINGESTRDMTHARAIELIKSGGRRVRLLLKRGTGQVPEYDEPAPWSSPAAAAPGLPEVGV
  SLDDGLAPFSPSHPAPPSDPSHQISPGPTWDIKREHDVRKPKELSACGQKKQRLGEQRERSASPQRAAR
  PRLEEAPGGQGRPEAGRPASEARAPGLAAADAADAARAGGKEAPRAAAGSELCRREGPGAAPAFAGPGG
  GGSGALEAEGRAGARAGPRPGPRPPGGAPARKAAVAPGPWKVPGSDKLPSVLKPGASAASR
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001301128
• ORF Size: 4323 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001301128.2
• RefSeq Size: 6845 bp
• RefSeq ORF: 4326 bp
• Locus ID: 9863
• Cytogenetics: 7q21.11
• Protein Families: Druggable Genome
• Protein Pathways: Tight junction
• MW: 157.7 kDa
• Gene Summary: The protein encoded by this gene interacts with atrophin-1. Atrophin-1 contains a polyglutamine repeat, expansion of which is responsible for dentatorubral and pallidoluysian atrophy. This encoded protein is characterized by two WW domains, a guanylate kinase-like domain, and multiple PDZ domains. It has structural similarity to the membrane-associated guanylate kinase homologue (MAGUK) family. [provided by RefSeq, Jul 2008]