SLIT2 (NM_001289136) Human Tagged ORF Clone

CAT#: RG240170

SLIT2 (GFP-tagged) - Human slit homolog 2 (Drosophila) (SLIT2), transcript variant 3

Specifications

Product Data

• Tag: TurboGFP
• Symbol: SLIT2
• Synonyms: SLIL3; Slit-2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG240170 representing NM_001289136.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGCGCGGCGTTGGCTGGCAGATGCTGTCCCTGTCGCTGGGGTTAGTGCTGGCGATCCTGAACAAGGTG
  GCACCGCAGGCGTGCCCGGCGCAGTGCTCTTGCTCGGGCAGCACAGTGGACTGTCACGGGCTGGCGCTG
  CGCAGCGTGCCCAGGAATATCCCCCGCAACACCGAGAGACTGGATTTAAATGGAAATAACATCACAAGA
  ATTACGAAGACAGATTTTGCTGGTCTTAGACATCTAAGAGTTCTTCAGCTTATGGAGAATAAGATTAGC
  ACCATTGAAAGAGGAGCATTCCAGGATCTTAAAGAACTAGAGAGACTGCGTTTAAACAGAAATCACCTT
  CAGCTGTTTCCTGAGTTGCTGTTTCTTGGGACTGCGAAGCTATACAGGCTTGATCTCAGTGAAAACCAA
  ATTCAGGCAATCCCAAGGAAAGCTTTCCGTGGGGCAGTTGACATAAAAAATTTGCAACTGGATTACAAC
  CAGATCAGCTGTATTGAAGATGGGGCATTCAGGGCTCTCCGGGACCTGGAAGTGCTCACTCTCAACAAT
  AACAACATTACTAGACTTTCTGTGGCAAGTTTCAACCATATGCCTAAACTTAGGACTTTTCGACTGCAT
  TCAAACAACCTGTATTGTGACTGCCACCTGGCCTGGCTCTCCGACTGGCTTCGCCAAAGGCCTCGGGTT
  GGTCTGTACACTCAGTGTATGGGCCCCTCCCACCTGAGAGGCCATAATGTAGCCGAGGTTCAAAAACGA
  GAATTTGTCTGCAGTGGTCACCAGTCATTTATGGCTCCTTCTTGTAGTGTTTTGCACTGCCCTGCCGCC
  TGTACCTGTAGCAACAATATCGTAGACTGTCGTGGGAAAGGTCTCACTGAGATCCCCACAAATCTTCCA
  GAGACCATCACAGAAATACGTTTGGAACAGAACACAATCAAAGTCATCCCTCCTGGAGCTTTCTCACCA
  TATAAAAAGCTTAGACGAATTGACCTGAGCAATAATCAGATCTCTGAACTTGCACCAGATGCTTTCCAA
  GGACTACGCTCTCTGAATTCACTTGTCCTCTATGGAAATAAAATCACAGAACTCCCCAAAAGTTTATTT
  GAAGGACTGTTTTCCTTACAGCTCCTATTATTGAATGCCAACAAGATAAACTGCCTTCGGGTAGATGCT
  TTTCAGGATCTCCACAACTTGAACCTTCTCTCCCTATATGACAACAAGCTTCAGACCATCGCCAAGGGG
  ACCTTTTCACCTCTTCGGGCCATTCAAACTATGCATTTGGCCCAGAACCCCTTTATTTGTGACTGCCAT
  CTCAAGTGGCTAGCGGATTATCTCCATACCAACCCGATTGAGACCAGTGGTGCCCGTTGCACCAGCCCC
  CGCCGCCTGGCAAACAAAAGAATTGGACAGATCAAAAGCAAGAAATTCCGTTGTTCAGGTACAGAAGAT
  TATCGATCAAAATTAAGTGGAGACTGCTTTGCGGATCTGGCTTGCCCTGAAAAGTGTCGCTGTGAAGGA
  ACCACAGTAGATTGCTCTAATCAAAAGCTCAACAAAATCCCGGAGCACATTCCCCAGTACACTGCAGAG
  TTGCGTCTCAATAATAATGAATTTACCGTGTTGGAAGCCACAGGAATCTTTAAGAAACTTCCTCAATTA
  CGTAAAATAAACTTTAGCAACAATAAGATCACAGATATTGAGGAGGGAGCATTTGAAGGAGCATCTGGT
  GTAAATGAAATACTTCTTACGAGTAATCGTTTGGAAAATGTGCAGCATAAGATGTTCAAGGGATTGGAA
  AGCCTCAAAACTTTGATGTTGAGAAGCAATCGAATAACCTGTGTGGGGAATGACAGTTTCATAGGACTC
  AGTTCTGTGCGTTTGCTTTCTTTGTATGATAATCAAATTACTACAGTTGCACCAGGGGCATTTGATACT
  CTCCATTCTTTATCTACTCTAAACCTCTTGGCCAATCCTTTTAACTGTAACTGCTACCTGGCTTGGTTG
  GGAGAGTGGCTGAGAAAGAAGAGAATTGTCACGGGAAATCCTAGATGTCAAAAACCATACTTCCTGAAA
  GAAATACCCATCCAGGATGTGGCCATTCAGGACTTCACTTGTGATGACGGAAATGATGACAATAGTTGC
  TCCCCACTTTCTCGCTGTCCTACTGAATGTACTTGCTTGGATACAGTCGTCCGATGTAGCAACAAGGGT
  TTGAAGGTCTTGCCGAAAGGTATTCCAAGAGATGTCACAGAGTTGTATCTGGATGGAAACCAATTTACA
  CTGGTTCCCAAGGAACTCTCCAACTACAAACATTTAACACTTATAGACTTAAGTAACAACAGAATAAGC
  ACGCTTTCTAATCAGAGCTTCAGCAACATGACCCAGCTCCTCACCTTAATTCTTAGTTACAACCGTCTG
  AGATGTATTCCTCCTCGCACCTTTGATGGATTAAAGTCTCTTCGATTACTTTCTCTACATGGAAATGAC
  ATTTCTGTTGTGCCTGAAGGTGCTTTCAATGATCTTTCTGCATTATCACATCTAGCAATTGGAGCCAAC
  CCTCTTTACTGTGATTGTAACATGCAGTGGTTATCCGACTGGGTGAAGTCGGAATATAAGGAGCCTGGA
  ATTGCTCGTTGTGCTGGTCCTGGAGAAATGGCAGATAAACTTTTACTCACAACTCCCTCCAAAAAATTT
  ACCTGTCAAGGTCCTGTGGATGTCAATATTCTAGCTAAGTGTAACCCCTGCCTATCAAATCCGTGTAAA
  AATGATGGCACATGTAATAGTGATCCAGTTGACTTTTACCGATGCACCTGTCCATATGGTTTCAAGGGG
  CAGGACTGTGATGTCCCAATTCATGCCTGCATCAGTAACCCATGTAAACATGGAGGAACTTGCCACTTA
  AAGGAAGGAGAAGAAGATGGATTCTGGTGTATTTGTGCTGATGGATTTGAAGGAGAAAATTGTGAAGTC
  AACGTTGATGATTGTGAAGATAATGACTGTGAAAATAATTCTACATGTGTCGATGGCATTAATAACTAC
  ACATGCCTTTGCCCACCTGAGTATACAGGTGAGTTGTGTGAGGAGAAGCTGGACTTCTGTGCCCAGGAC
  CTGAACCCCTGCCAGCACGATTCAAAGTGCATCCTAACTCCAAAGGGATTCAAATGTGACTGCACACCA
  GGGTACGTAGGTGAACACTGCGACATCGATTTTGACGACTGCCAAGACAACAAGTGTAAAAACGGAGCC
  CACTGCACAGATGCAGTGAACGGCTATACGTGCATATGCCCCGAAGGTTACAGTGGCTTGTTCTGTGAG
  TTTTCTCCACCCATGGTCCTCCCTCGTACCAGCCCCTGTGATAATTTTGATTGTCAGAATGGAGCTCAG
  TGTATCGTCAGAATAAATGAGCCAATATGTCAGTGTTTGCCTGGCTATCAGGGAGAAAAGTGTGAAAAA
  TTGGTTAGTGTGAATTTTATAAACAAAGAGTCTTATCTTCAGATTCCTTCAGCCAAGGTTCGGCCTCAG
  ACGAACATAACACTTCAGATTGCCACAGATGAAGACAGCGGAATCCTCCTGTATAAGGGTGACAAAGAC
  CATATCGCGGTAGAACTCTATCGGGGGCGTGTTCGTGCCAGCTATGACACCGGCTCTCATCCAGCTTCT
  GCCATTTACAGTGTGGAGACAATCAATGATGGAAACTTCCACATTGTGGAACTACTTGCCTTGGATCAG
  AGTCTCTCTTTGTCCGTGGATGGTGGGAACCCCAAAATCATCACTAACTTGTCAAAGCAGTCCACTCTG
  AATTTTGACTCTCCACTCTATGTAGGAGGCATGCCAGGGAAGAGTAACGTGGCATCTCTGCGCCAGGCC
  CCTGGGCAGAACGGAACCAGCTTCCACGGCTGCATCCGGAACCTTTACATCAACAGTGAGCTGCAGGAC
  TTCCAGAAGGTGCCGATGCAAACAGGCATTTTGCCTGGCTGTGAGCCATGCCACAAGAAGGTGTGTGCC
  CATGGCACATGCCAGCCCAGCAGCCAGGCAGGCTTCACCTGCGAGTGCCAGGAAGGATGGATGGGGCCC
  CTCTGTGACCAACGGACCAATGACCCTTGCCTTGGAAATAAATGCGTACATGGCACCTGCTTGCCCATC
  AATGCGTTCTCCTACAGCTGTAAGTGCTTGGAGGGCCATGGAGGTGTCCTCTGTGATGAAGAGGAGGAT
  CTGTTTAACCCATGCCAGGCGATCAAGTGCAAGCATGGGAAGTGCAGGCTTTCAGGTCTGGGGCAGCCC
  TACTGTGAATGCAGCAGTGGATACACGGGGGACAGCTGTGATCGAGAAATCTCTTGTCGAGGGGAAAGG
  ATAAGAGATTATTACCAAAAGCAGCAGGGCTATGCTGCTTGCCAAACAACCAAGAAGGTGTCCCGATTA
  GAGTGCAGAGGTGGGTGTGCAGGAGGGCAGTGCTGTGGACCGCTGAGGAGCAAGCGGCGGAAATACTCT
  TTCGAATGCACTGACGGCTCCTCCTTTGTGGACGAGGTTGAGAAAGTGGTGAAGTGCGGCTGTACGAGG
  TGTGTGTCC
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG240170
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MRGVGWQMLSLSLGLVLAILNKVAPQACPAQCSCSGSTVDCHGLALRSVPRNIPRNTERLDLNGNNITR
  ITKTDFAGLRHLRVLQLMENKISTIERGAFQDLKELERLRLNRNHLQLFPELLFLGTAKLYRLDLSENQ
  IQAIPRKAFRGAVDIKNLQLDYNQISCIEDGAFRALRDLEVLTLNNNNITRLSVASFNHMPKLRTFRLH
  SNNLYCDCHLAWLSDWLRQRPRVGLYTQCMGPSHLRGHNVAEVQKREFVCSGHQSFMAPSCSVLHCPAA
  CTCSNNIVDCRGKGLTEIPTNLPETITEIRLEQNTIKVIPPGAFSPYKKLRRIDLSNNQISELAPDAFQ
  GLRSLNSLVLYGNKITELPKSLFEGLFSLQLLLLNANKINCLRVDAFQDLHNLNLLSLYDNKLQTIAKG
  TFSPLRAIQTMHLAQNPFICDCHLKWLADYLHTNPIETSGARCTSPRRLANKRIGQIKSKKFRCSGTED
  YRSKLSGDCFADLACPEKCRCEGTTVDCSNQKLNKIPEHIPQYTAELRLNNNEFTVLEATGIFKKLPQL
  RKINFSNNKITDIEEGAFEGASGVNEILLTSNRLENVQHKMFKGLESLKTLMLRSNRITCVGNDSFIGL
  SSVRLLSLYDNQITTVAPGAFDTLHSLSTLNLLANPFNCNCYLAWLGEWLRKKRIVTGNPRCQKPYFLK
  EIPIQDVAIQDFTCDDGNDDNSCSPLSRCPTECTCLDTVVRCSNKGLKVLPKGIPRDVTELYLDGNQFT
  LVPKELSNYKHLTLIDLSNNRISTLSNQSFSNMTQLLTLILSYNRLRCIPPRTFDGLKSLRLLSLHGND
  ISVVPEGAFNDLSALSHLAIGANPLYCDCNMQWLSDWVKSEYKEPGIARCAGPGEMADKLLLTTPSKKF
  TCQGPVDVNILAKCNPCLSNPCKNDGTCNSDPVDFYRCTCPYGFKGQDCDVPIHACISNPCKHGGTCHL
  KEGEEDGFWCICADGFEGENCEVNVDDCEDNDCENNSTCVDGINNYTCLCPPEYTGELCEEKLDFCAQD
  LNPCQHDSKCILTPKGFKCDCTPGYVGEHCDIDFDDCQDNKCKNGAHCTDAVNGYTCICPEGYSGLFCE
  FSPPMVLPRTSPCDNFDCQNGAQCIVRINEPICQCLPGYQGEKCEKLVSVNFINKESYLQIPSAKVRPQ
  TNITLQIATDEDSGILLYKGDKDHIAVELYRGRVRASYDTGSHPASAIYSVETINDGNFHIVELLALDQ
  SLSLSVDGGNPKIITNLSKQSTLNFDSPLYVGGMPGKSNVASLRQAPGQNGTSFHGCIRNLYINSELQD
  FQKVPMQTGILPGCEPCHKKVCAHGTCQPSSQAGFTCECQEGWMGPLCDQRTNDPCLGNKCVHGTCLPI
  NAFSYSCKCLEGHGGVLCDEEEDLFNPCQAIKCKHGKCRLSGLGQPYCECSSGYTGDSCDREISCRGER
  IRDYYQKQQGYAACQTTKKVSRLECRGGCAGGQCCGPLRSKRRKYSFECTDGSSFVDEVEKVVKCGCTR
  CVS
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001289136
• ORF Size: 4563 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001289136.3
• RefSeq Size: 6374 bp
• RefSeq ORF: 4566 bp
• Locus ID: 9353
• Cytogenetics: 4p15.31
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Axon guidance
• MW: 169.3 kDa
• Gene Summary: This gene encodes a member of the slit family of secreted glycoproteins, which are ligands for the Robo family of immunoglobulin receptors. Slit proteins play highly conserved roles in axon guidance and neuronal migration and may also have functions during other cell migration processes including leukocyte migration. Members of the slit family are characterized by an N-terminal signal peptide, four leucine-rich repeats, nine epidermal growth factor repeats, and a C-terminal cysteine knot. Proteolytic processing of this protein gives rise to an N-terminal fragment that contains the four leucine-rich repeats and five epidermal growth factor repeats and a C-terminal fragment that contains four epidermal growth factor repeats and the cysteine knot. Both full length and cleaved proteins are secreted extracellularly and can function in axon repulsion as well as other specific processes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]