l Afadin (AFDN) (NM_001291964) Human Tagged ORF Clone

CAT#: RG240207

MLLT4 (GFP-tagged) - Human myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila), translocated to, 4 (MLLT4), transcript variant 4

Specifications

Product Data

• Tag: TurboGFP
• Symbol: AFDN
• Synonyms: AF6; l-afadin; MLL-AF6; MLLT4
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG240207 representing NM_001291964.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGAGATTTTATTTTCAAGATAAAGCTGCTGGAAACTTTGCAACAAAATGTATTCGGGTCTCTAGTACT
  GCCACCACTCAAGATGTAATCGAAACGCTCGCGGAGAAATTTCGACCTGATATGCGAATGCTGTCCTCT
  CCCAAGTATTCACTCTATGAAGTGCATGTCAGCGGAGAAGAAAGAAGATTGGATATAGATGAGAAACCT
  CTAGTTGTACAACTGAATTGGAACAAAGATGATCGGGAAGGCAGATTTGTTCTTAAGAATGAGAATGAC
  GCCATTCCTCCTAAGAAGGCTCAAAGTAATGGACCTGAAAAGCAGGAAAAAGAAGGGGTTATCCAGAAC
  TTCAAGAGAACTCTCTCAAAGAAAGAAAAGAAGGAAAAAAAGAAGAGAGAAAAAGAGGCATTGCGACAG
  GCATCTGATAAAGATGATAGACCTTTCCAAGGGGAGGATGTTGAAAATTCTCGACTGGCTGCTGAGGTT
  TACAAAGACATGCCGGAAACCAGCTTTACTCGAACCATTTCTAATCCTGAGGTGGTTATGAAACGACGG
  AGGCAGCAAAAATTGGAAAAGAGAATGCAGGAATTTCGGAGCTCAGATGGGCGGCCTGATTCAGGTGGA
  ACATTGAGAATTTATGCAGATAGTTTAAAACCAAATATTCCCTACAAGACAATCCTGCTGTCTACTACA
  GATCCTGCAGACTTTGCTGTGGCTGAAGCTTTAGAGAAGTATGGTCTGGAAAAAGAAAACCCTAAGGAT
  TACTGCATCGCCCGGGTTATGCTTCCTCCTGGAGCCCAGCATTCTGATGAAAAGGGTGCTAAAGAAATT
  ATTCTTGATGATGATGAGTGTCCTTTACAAATCTTCAGGGAATGGCCAAGTGACAAAGGGATTTTAGTC
  TTTCAGTTGAAGAGGAGGCCACCAGACCACATCCCAAAGAAAACCAAGAAACACTTGGAAGGCAAGACA
  CCCAAGGGAAAGGAGAGAGCTGACGGGTCTGGCTATGGCTCCACCCTTCCTCCGGAGAAGCTGCCCTAT
  TTAGTAGAGTTAAGCCCAGGGAGAAGGAATCACTTTGCCTACTACAACTATCACACTTACGAAGATGGT
  TCTGACTCTAGAGATAAGCCAAAGCTTTACCGCCTTCAGTTAAGTGTTACTGAAGTTGGGACAGAAAAG
  TTGGATGACAACTCTATCCAGTTGTTTGGCCCAGGAATTCAGCCCCATCACTGTGACCTTACCAACATG
  GATGGAGTGGTCACTGTGACGCCCAGAAGTATGGACGCAGAAACCTACGTGGAAGGCCAGCGCATCTCA
  GAAACCACCATGCTGCAGAGTGGCATGAAAGTGCAGTTTGGGGCGTCCCATGTATTTAAGTTTGTGGAC
  CCCAGTCAGGATCATGCTCTTGCAAAAAGATCTGTGGATGGAGGCCTGATGGTTAAGGGCCCAAGACAT
  AAACCTGGAATTGTTCAGGAGACAACTTTTGATTTGGGAGGAGATATTCATAGTGGGACAGCATTACCG
  ACAAGCAAGAGCACCACTAGGCTGGACAGCGACAGAGTGTCGTCTGCCTCTAGCACAGCCGAGCGGGGA
  ATGGTGAAGCCGATGATCAGAGTAGAACAGCAGCCAGATTATCGCAGGCAAGAAAGCAGAACACAGGAT
  GCTTCTGGGCCTGAGCTGATACTACCTGCAAGCATTGAATTCAGGGAAAGTTCTGAAGATTCATTTTTG
  TCTGCCATTATAAATTATACTAATAGCTCTACAGTCCACTTTAAGTTGTCCCCTACATATGTATTATAT
  ATGGCATGCCGGTATGTATTGTCCAACCAGTACAGACCTGACATCAGCCCTACAGAGCGCACACATAAA
  GTCATTGCAGTCGTCAACAAGATGGTGAGCATGATGGAGGGTGTCATCCAGAAACAGAAGAATATTGCA
  GGGGCACTTGCCTTCTGGATGGCAAATGCATCTGAACTTCTCAACTTCATTAAGCAAGACCGAGACCTT
  AGTCGGATCACACTGGATGCTCAAGATGTTTTAGCACATTTGGTTCAAATGGCATTTAAATACTTGGTT
  CACTGTCTTCAATCAGAACTTAATAATTACATGCCAGCCTTTCTAGATGACCCTGAAGAGAACAGTCTG
  CAACGACCAAAAATAGATGATGTGCTGCACACGCTCACAGGAGCCATGTCCTTGCTACGACGCTGCAGA
  GTCAATGCCGCCCTGACCATCCAGCTCTTCTCTCAGCTCTTCCACTTCATCAATATGTGGCTGTTCAAT
  AGATTGGTGACCGACCCAGATTCGGGGCTGTGCTCCCATTACTGGGGTGCGATTATCCGTCAGCAGTTG
  GGCCATATTGAAGCCTGGGCTGAGAAGCAGGGGCTGGAACTGGCTGCGGACTGTCATCTGAGCAGGATC
  GTGCAGGCAACGACTTTGCTTACCATGGATAAGTATGCACCTGATGACATTCCAAATATAAACAGCACC
  TGCTTTAAGTTAAATTCATTACAACTTCAAGCCTTATTACAGAACTATCACTGTGCACCTGATGAGCCT
  TTTATCCCAACGGATCTTATAGAAAATGTAGTGACTGTGGCTGAAAACACTGCCGATGAGCTGGCCCGC
  AGTGATGGAAGGGAAGTGCAGTTGGAGGAGGATCCTGATCTGCAGCTGCCGTTTCTTTTGCCAGAAGAT
  GGTTATTCTTGTGATGTTGTCAGAAACATTCCAAATGGTTTACAAGAATTTTTAGACCCTCTGTGCCAG
  AGAGGATTTTGCAGGTTAATTCCTCACACACGTTCACCAGGTACTTGGACAATATATTTTGAAGGTGCA
  GATTATGAAAGTCACCTTCTGCGTGAGAACACAGAGCTGGCTCAGCCTCTGAGGAAAGAACCTGAAATA
  ATCACTGTGACCCTAAAAAAGCAGAATGGAATGGGCCTTAGCATTGTTGCAGCAAAGGGTGCTGGTCAA
  GATAAACTAGGAATCTATGTGAAGTCGGTTGTGAAAGGAGGTGCTGCAGATGTGGATGGACGTCTAGCT
  GCAGGTGATCAGCTCCTCAGTGTGGATGGACGAAGTCTGGTTGGACTCTCTCAGGAAAGGGCGGCAGAA
  CTCATGACAAGAACAAGCTCTGTGGTGACACTGGAAGTAGCAAAGCAGGGTGCCATCTACCACGGTCTG
  GCCACCCTTCTCAATCAGCCATCCCCCATGATGCAGAGAATTTCAGATCGTCGTGGCTCAGGTAAACCC
  CGACCAAAGAGTGAAGGCTTTGAGCTCTATAATAATTCAACTCAAAATGGGTCTCCTGAGAGTCCTCAG
  CTGCCTTGGGCAGAATATAGTGAACCAAAGAAATTGCCTGGTGATGACAGACTGATGAAAAATAGAGCT
  GATCACCGTTCCAGCCCCAACGTAGCAAATCAGCCTCCTAGTCCTGGAGGGAAAAGTGCATATGCCTCT
  GGAACAACAGCGAAGATAACATCTGTCTCTACTGGAAACCTCTGCACTGAGGAGCAGACGCCTCCGCCT
  AGACCTGAAGCCTACCCCATCCCCACTCAGACGTACACCAGAGAGTATTTTACCTTCCCAGCTTCCAAA
  TCCCAGGATCGGATGGCTCCTCCTCAGAACCAGTGGCCAAATTATGAGGAAAAGCCACATATGCACACA
  GATAGTAATCATTCCAGTATTGCAATTCAGCGTGTTACACGTTCCCAAGAAGAACTTCGAGAAGATAAA
  GCTTACCAACTTGAGCGGCATCGAATAGAGGCAGCTATGGACCGAAAGTCTGATAGTGATATGTGGATA
  AATCAGAGCTCCTCACTGGACTCCAGTACCTCTAGCCAGGAGCATCTGAACCATTCCTCTAAGTCGGTC
  ACCCCTGCTTCCACACTGACCAAAAGTGGCCCTGGCCGTTGGAAAACACCAGCAGCCATACCGGCCACC
  CCTGTGGCCGTCTCCCAGCCAATCCGAACAGACCTGCCTCCGCCACCCCCGCCACCTCCAGTCCACTAT
  GCCGGTGATTTCGATGGAATGTCCATGGATTTGCCTCTCCCACCACCCCCTTCCGCCAACCAGATAGGG
  CTGCCGTCTGCGCAGGTGGCTGCTGCTGAACGGAGAAAGAGAGAAGAACATCAGCGTTGGTATGAGAAG
  GAGAAGGCCCGCCTGGAGGAGGAGCGGGAGAGGAAGCGGAGAGAGCAGGAGAGGAAGTTGGGCCAGATG
  CGCACTCAGTCCTTAAACCCTGCTCCGTTTTCTCCCCTGACTGCACAGCAGATGAAGCCCGAAAAGCCT
  TCCACACTCCAGCGGCCACAGGAAACAGTCATTCGGGAGCTGCAGCCTCAGCAGCAGCCCCGCACGATC
  GAGCGCAGAGACTTGCAGTACATTACAGTCAGCAAAGAGGAGCTTTCCTCGGGGGACAGTCTGTCCCCC
  GACCCGTGGAAGCGGGACGCCAAGGAGAAGCTGGAGAAGCAGCAGCAGATGCACATCGTGGACATGCTG
  AGCAAGGAGATCCAGGAGCTCCAGAGCAAACCGGACCGCAGCGCCGAGGAGAGCGACCGGCTGCGCAAG
  CTCATGCTGGAGTGGCAGTTCCAGAAGAGACTCCAGGAGTCGAAGCAGAAGGACGAAGATGACGAGGAG
  GAGGAGGACGATGATGTGGACACCATGCTGATCATGCAGCGCCTGGAGGCTGAACGAAGAGCGAGGGAC
  GAGGAGCGGAGGCGGCAGCAGCAGTTAGAAGAGATGCGCAAGCGGGAAGCGGAAGACCGAGCGAGGCAA
  GAGGAAGAGCGCCGGCGGCAGGAGGAGGAGCGAACAAAACGAGACGCTGAAGAAAAGAGGCGACAGGAA
  GAAGGGTATTACAGCCGCCTGGAAGCCGAGAGGCGCAGACAGCACGACGAGGCGGCGCGCAGGTTGCTG
  GAGCCCGAGGCGCCCGGTCTGTGCCGCCCTCCGCTTCCCCGGGACTACGAGCCCCCGTCCCCGTCCCCC
  GCGCCCGGCGCCCCTCCTCCCCCGCCTCAGCGAAACGCCTCCTACCTCAAAACACAGGTCCTCTCCCCC
  GACTCGCTGTTCACTGCCAAGTTTGTTGCATACAATGAGGAGGAGGAGGAGGAGGACTGCAGCCTAGCA
  GGACCAAACTCTTACCCAGGATCTACTGGAGCAGCTGTTGGAGCCCATGACGCCTGTCGGGATGCAAAA
  GAGAAGCGCTCTAAAAGCCAAGATGCAGATTCACCTGGAAGTTCTGGGGCCCCTGAAAACTTGACATTC
  AAGGAACGCCAGCGTCTTTTTTCACAAGGTCAAGATGTATCCAATAAAGTGAAAGCTTCTCGTAAATTA
  ACAGAACTGGAGAATGAACTGAACACAAAG
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG240207
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKP
  LVVQLNWNKDDREGRFVLKNENDAIPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQ
  ASDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGG
  TLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEI
  ILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKTPKGKERADGSGYGSTLPPEKLPY
  LVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHCDLTNM
  DGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLMVKGPRH
  KPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASSTAERGMVKPMIRVEQQPDYRRQESRTQD
  ASGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHK
  VIAVVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLV
  HCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFN
  RLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINST
  CFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVTVAENTADELARSDGREVQLEEDPDLQLPFLLPED
  GYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHTRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEI
  ITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAE
  LMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSTQNGSPESPQ
  LPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYASGTTAKITSVSTGNLCTEEQTPPP
  RPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHTDSNHSSIAIQRVTRSQEELREDK
  AYQLERHRIEAAMDRKSDSDMWINQSSSLDSSTSSQEHLNHSSKSVTPASTLTKSGPGRWKTPAAIPAT
  PVAVSQPIRTDLPPPPPPPPVHYAGDFDGMSMDLPLPPPPSANQIGLPSAQVAAAERRKREEHQRWYEK
  EKARLEEERERKRREQERKLGQMRTQSLNPAPFSPLTAQQMKPEKPSTLQRPQETVIRELQPQQQPRTI
  ERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRLRK
  LMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARDEERRRQQQLEEMRKREAEDRARQ
  EEERRRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHDEAARRLLEPEAPGLCRPPLPRDYEPPSPSP
  APGAPPPPPQRNASYLKTQVLSPDSLFTAKFVAYNEEEEEEDCSLAGPNSYPGSTGAAVGAHDACRDAK
  EKRSKSQDADSPGSSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001291964
• ORF Size: 5343 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001291964.1, NP_001278893.1
• RefSeq Size: 7750 bp
• RefSeq ORF: 5346 bp
• Locus ID: 4301
• Cytogenetics: 6q27
• Protein Families: Druggable Genome
• Protein Pathways: Adherens junction, Leukocyte transendothelial migration, Tight junction
• MW: 202.3 kDa
• Gene Summary: 'This gene encodes a multi-domain protein involved in signaling and organization of cell junctions during embryogenesis. It has also been identified as the fusion partner of acute lymphoblastic leukemia (ALL-1) gene, involved in acute myeloid leukemias with t(6;11)(q27;q23) translocation. Alternatively spliced transcript variants encoding different isoforms have been described for this gene, however, not all have been fully characterized.[provided by RefSeq, May 2011]'