GARNL1 (RALGAPA1) (NM_001283043) Human Tagged ORF Clone

CAT#: RG240229

RALGAPA1 (GFP-tagged) - Human Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant 3

Specifications

Product Data

• Tag: TurboGFP
• Symbol: RALGAPA1
• Synonyms: GARNL1; GRIPE; p240; RalGAPalpha1; TULIP1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG240229 representing NM_001283043.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGTTCTCCAAGAAGCCGCACGGGGACGTGAAGAAGTCCACCCAGAAGGTGCTAGACACCAAGAAGGAC
  GCACTGACTCGCCTCAAGCACCTGCGCATCGTCATCGAGAATGCAGAATCTATTGATCTTAAACAGTTT
  TTCGACCAACATTTTTCACATATATACTATGTGTTCTTTGAAAATTTTGTGACTATTGAAGCTAGTCTT
  AAACAGAAAGGTCACAAGTCTCAAAGAGAGGAATTGGATGCTATACTTTTTATTTTTGAGAAAATTTTA
  CAACTTCTTCCAGAAAGAATTCATCAGCGATGGCAGTTTCATAGTATTGGATTGATTTTAAAGAAGCTA
  CTTCACACAGGAAACTCCTTAAAGATTAGGCGGGAAGGTGTTCGTCTTTTCTTACTATGGTTGCAAGCT
  CTTCAGAATAACTGTAGCAAAGAACAGCTCTGGATGTTTTCATGCTTAATCCCTGGATTTTCAGCACCA
  CAGTCTGAACATGGACCTCGAACTTTAGATAATCTCATTAATCCTCCACTCAACCTTCAAGAAACTCAA
  GTCACTATAGAAGAAATCACTCCTCTTGTCCCCCCACAATCAGGAGATAAAGGGCAAGAAGATCTCACA
  AGCTATTTTCTTGAAGCACTTCTAAAATACATAGTCATTCAGGTAAAAAGTTTAGAATGGAAGAACAAA
  GAAAACCAAGAAAGGGGATTTTCATTTTTGTTTTCACATTTTAAGAAATATTACTTGCCTTATATTTTT
  CCAAACATCTGTAAGGAAAACAGTTTATATCATCCTATACTTGACATCCCGCAGATGAGACCAAAGCCA
  CATTATGTCGTGATAAAGAAAGATGCTGAAACCAATGAAGCAATCTATTGTACAAAGGAGCCTTTCATT
  AAGGCTCGTGTTATTGTCATTCGTTGGCTGGTTTCTTTCTGGCTGGAGCCAAAACCACATACAGGACCT
  CATATTCCTGGGATGGAAGGTGAAGTCTTGCCAAAGAATATTCAGAGAGCAGCTGCTAGTTTAGTATCC
  AGAGAAGAAAGCAAAAATGATAATGCTGATAAAACAGACAGAACTACAGAACCCGAACAGTCTCATTCC
  AATACAAGCACTCTCACGGAGCGAGAACCTAGCTCATCTAGTCTCTGTAGTATTGATGAAGAACATCTC
  ACAGACATTGAAATAGTTCGCAGAGTTTTTTCTTCTAAAAGGAGTAATGTAAACTTTGTGACAGAGATA
  TTTCGTCAGGCATTTTTATTACCAATTTGTGAAGCAGCAGCTATGAGAAAAGTGGTAAAAGTATATCAA
  GAATGGATCCAACAAGAGGAAAAACCTTTGTTCATGCAAGAGCCTGAAGAAATTGTGATCACTTCTTCA
  GACCTCCCTTGCATTGAAAATGTCACAGACCATGATATTTCAATGGAAGAAGGAGAAAAAAGAGAAGAG
  GAAAATGGGACCAATACTGCTGATCATGTTCGAAATTCCAGTTGGGCAAAAAACGGCTCCTACCAAGGT
  GCTCTTCATAACGCCTCTGAAGAAGCCACAGAACAAAACATACGAGCTGGTACCCAGGCAGTTTTGCAG
  GTGTTTATTATAAACTCATCAAATATATTTCTTCTTGAACCTGCAAATGAAATAAAAAATCTTCTGGAT
  GAACACACAGATATGTGTAAACGCATTCTTAACATTTATCGGTACATGGTTGTACAAGTATCAATGGAC
  AAAAAGACTTGGGAACAGATGCTGCTTGTGTTGCTCAGAGTCACGGAATCTGTACTGAAGATGCCATCA
  CAAGCTTTTCTACAGTTCCAAGGGAAAAAAAATATGACCTTGGCAGGTCGACTTGCAGGACCACTTTTC
  CAGACCCTTATAGTTGCCTGGATCAAAGCAAACCTAAATGTGTACATCTCCCGAGAACTTTGGGATGAC
  TTACTGTCAGTATTGTCATCGCTGACCTATTGGGAAGAGTTGGCCACTGAGTGGTCACTGACTATGGAG
  ACATTAACTAAAGTTTTAGCTAGGAATTTATATAGTTTGGATCTCAGTGATTTACCATTGGATAAGCTG
  AGTGAACAGAAACAAAAAAAGCACAAAGGGAAAGGAGTTGGACATGAATTTCAGAAAGTTTCAGTTGAC
  AAGTCATTTTCTAGAGGATGGAGTCGTGATCAGCCTGGCCAAGCCCCAATGAGACAGAGGAGTGCAACA
  ACCACTGGTTCTCCAGGAACCGAAAAGGCGAGGAGTATAGTACGGCAAAAAACTGTCGATATTGATGAT
  GCTCAAATACTTCCCCGCTCAACTAGAGTCAGACATTTTTCACAAAGTGAAGAAACTGGAAATGAAGTT
  TTTGGTGCTTTGAATGAGGAGCAGCCATTGCCTCGAAGTAGCAGCACTTCTGACATCTTGGAACCATTC
  ACTGTTGAACGAGCCAAAGAGGCAGAACAGAATGCCACCAGAGGCTCCACTGAGGGATCTGTACAGTCA
  TGTAATGGGCTATTTTGGAAGGAAAGCTGCAGTGATATTGGCGGCAGCAGTGCTAATGTTCCTGATCTG
  ATGGATGAGTTTATAGCAGAACGACTTCGAAGTGGTAATGCCTCGACTATGACAAGAAGAGGAAGTAGT
  CCAGGCAGCCTGGAAATTCCCAAAGACCTCCCTGATATTCTAAACAAGCAGAACCAGATGCGCCCTATT
  GATGACCCAGGTGTGCCCTCAGAATGGACTTCTCCTGCCAGTGCAGGGAGCAGTGATCTTATCAGCTCA
  GATAGTCATTCGGATTCTTTCAGCGCTTTCCAATATGATGGCCGAAAATTTGACAATTTTGGCTTTGGA
  ACCGACACTGGGGTTACGTCCTCTGCTGATGTGGATTCAGGTTCTGGCCATCATCAGAGTGCTGAAGAG
  CAGGAAGTGGCTAGTCTAACTACTCTTCATATAGATTCTGAAACAAGCAGTCTTAATCAGCAAGCTTTC
  TCTGCTGAAGTTGCAACTATTACTGGTTCAGAAAGTGCTTCCCCAGTCCACTCACCTCTGGGCTCCAGG
  TCACAGACTCCTTCCCCTTCTACATTGAATATAGATCACATGGAACAGAAGGATCTGCAGCTCGACGAG
  AAGCTCCACCACTCTGTTCTTCAGACACCAGATGATCTAGAAATTAGTGAATTTCCATCAGAATGTTGT
  AGTGTGATGGCAGGAGGTACTCTGACTGGATGGCATGCTGATGTTGCTACTGTAATGTGGCGAAGAATG
  CTAGGCATTTTGGGAGATGTAAATTCAATCATGGATCCTGAAATACATGCTCAAGTTTTTGATTACCTC
  TGTGAACTTTGGCAGAATCTAGCTAAGATTAGAGATAACCTTGGCATTTCAACTGATAACCTGACCTCC
  CCTTCTCCACCAGTTTTAATTCCTCCACTGAGAATTCTTACACCTTGGCTTTTTAAGGCAACCATGTTG
  ACTGATAAATATAAACAAGGTAAATTACATGCATATAAACTTATTTGTAATACAATGAAAAGAAGACAA
  GATGTTTCTCCAAATAGAGATTTTCTAACACATTTCTACAATATAATGCATTGTGGATTACTTCATATT
  GACCAGGATATTGTCAATACAATCATCAAACACTGCTCACCTCAATTTTTTTCACTTGGTTTGCCTGGT
  GCCACAATGCTTATTATGGATTTTATTGTAGCAGCTGGTAGAGTGGCTTCTTCAGCTTTTCTCAATGCA
  CCAAGAGTAGAAGCACAAGTTCTTCTGGGATCTTTGGTTTGCTTTCCCAACTTATATTGTGAACTGCCT
  TCTCTTCATCCCAACATTCCTGATGTTGCTGTGTCTCAGTTTACAGATGTTAAGGAACTTATAATCAAA
  ACTGTATTAAGCTCGGCAAGAGATGAGCCCTCTGGTCCTGCACGATGTGTAGCACTTTGTAGTTTAGGT
  ATTTGGATTTGTGAAGAACTAGTCCATGAGTCTCATCATCCTCAAATTAAGGAAGCTCTGAATGTGATT
  TGTGTTTCCTTAAAGTTTACTAATAAAACAGTAGCCCACGTAGCTTGTAACATGCTTCACATGCTGGTT
  CATTATGTACCTAGACTTCAGATTTACCAGCCTGATTCTCCCTTGAAAATTATTCAAATCCTAATAGCT
  ACCATCACCCATCTTTTACCAAGTACAGAGGCTTCATCTTATGAAATGGACAAGAGGTTGGTAGTATCT
  TTACTTCTCTGCCTTCTGGACTGGATCATGGCCTTACCTCTAAAGACACTGCTCCAACCATTTCATGCT
  ACGGGAGCAGAAAGCGATAAAACAGAAAAATCTGTTCTCAATTGCATTTATAAGGTTTTACATGGGTGT
  GTTTATGGAGCTCAGTGTTTTAGCAATCCAAGGTATTTTCCCATGAGCCTCTCTGATTTGGCATCTGTA
  GATTATGATCCTTTTATGCATTTGGAAAGTCTGAAAGAGCCTGAGCCTCTGCACTCTCCTGACTCAGAA
  CGATCTTCTAAACTCCAGCCAGTAACAGAAGTGAAAACTCAAATGCAGCATGGATTAATCTCTATAGCA
  GCCCGCACTGTTATTACACATCTGGTAAATCACCTGGGCCATTATCCAATGAGCGGTGGTCCTGCTATG
  CTAACAAGTCAGGTGTGTGAAAATCACGACAATCATTACAGTGAAAGTACTGAACTTTCTCCTGAACTC
  TTTGAGAGTCCAAATATCCAGTTCTTTGTGTTAAATAATACAACCTTAGTGTCCTGTATCCAGATCAGA
  TCAGAAGAGAATATGCCTGGAGGAGGTTTATCTGCTGGCCTTGCATCAGCCAATTCAAATGTCAGAATC
  ATAGTACGTGATCTCTCTGGAAAATATTCATGGGATTCTGCTATACTGTATGGCCCACCTCCTGTAAGT
  GGCTTGTCAGAACCTACATCTTTCATGCTTTCATTGTCTCACCAAGAGAAGCCAGAAGAGCCTCCGACA
  TCTAATGAATGCTTAGAAGATATAACCGTAAAAGATGGACTTTCTCTCCAGTTTAAAAGATTTAGAGAA
  ACTGTACCAACTTGGGATACAATAAGAGATGAAGAAGATGTTCTTGATGAGCTCTTGCAGTATTTGGGT
  GTTACTAGTCCTGAATGCTTACAGAGAACTGGAATCTCACTTAATATTCCTGCTCCACAACCTGTGTGC
  ATTTCTGAAAAACAAGAAAATGATGTTATTAATGCTATCCTTAAGCAACATACAGAAGAAAAAGAATTT
  GTTGAGAAGCACTTTAATGACTTAAACATGAAAGCTGTGGAACAAGATGAACCAATACCTCAAAAACCT
  CAGTCAGCATTTTATTATTGCAGATTGCTTCTTAGTATATTGGGAATGAATTCCTGGGACAAACGGAGG
  AGCTTTCATCTCCTGAAGAAAAATGAAAAGCTACTTAGAGAACTTAGGAACTTGGATTCAAGGCAGTGC
  CGAGAGACACACAAGATTGCAGTATTTTATGTTGCTGAAGGACAAGAAGACAAACACTCCATTCTCACC
  AATACAGGAGGAAGTCAAGCATATGAAGATTTTGTAGCTGGTCTTGGTTGGGAGGTAAATCTTACAAAC
  CATTGTGGTTTTATGGGAGGACTACAAAAAAACAAAAGCACTGGATTGACCACTCCATATTTTGCTACC
  TCTACAGTAGAGGTAATATTTCACGTGTCAACAAGAATGCCTTCTGATTCTGATGATTCTTTGACCAAA
  AAATTGAGACATTTGGGAAATGATGAAGTGCACATTGTTTGGTCAGAGCATACTAGAGACTACAGGAGA
  GGAATTATTCCCACAGAATTTGGTGATGTCCTTATTGTAATATATCCAATGAAAAATCACATGTTCAGT
  ATTCAGATAATGAAAAAACCAGAGGTTCCCTTCTTTGGTCCCCTTTTTGATGGTGCTATTGTGAATGGA
  AAGGTTCTACCCATTATGGTTAGAGCAACAGCTATAAATGCAAGCCGTGCTCTGAAATCTCTGATTCCA
  TTGTATCAAAACTTCTATGAGGAGAGAGCACGATACCTGCAAACAATTGTCCAGCACCACTTAGAACCA
  ACAACATTTGAAGATTTTGCAGCACAGGTTTTTTCTCCAGCTCCCTACCACCATTTACCATCTGATGCC
  GATCAT
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG240229
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MFSKKPHGDVKKSTQKVLDTKKDALTRLKHLRIVIENAESIDLKQFFDQHFSHIYYVFFENFVTIEASL
  KQKGHKSQREELDAILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQA
  LQNNCSKEQLWMFSCLIPGFSAPQSEHGPRTLDNLINPPLNLQETQVTIEEITPLVPPQSGDKGQEDLT
  SYFLEALLKYIVIQVKSLEWKNKENQERGFSFLFSHFKKYYLPYIFPNICKENSLYHPILDIPQMRPKP
  HYVVIKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHTGPHIPGMEGEVLPKNIQRAAASLVS
  REESKNDNADKTDRTTEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRRVFSSKRSNVNFVTEI
  FRQAFLLPICEAAAMRKVVKVYQEWIQQEEKPLFMQEPEEIVITSSDLPCIENVTDHDISMEEGEKREE
  ENGTNTADHVRNSSWAKNGSYQGALHNASEEATEQNIRAGTQAVLQVFIINSSNIFLLEPANEIKNLLD
  EHTDMCKRILNIYRYMVVQVSMDKKTWEQMLLVLLRVTESVLKMPSQAFLQFQGKKNMTLAGRLAGPLF
  QTLIVAWIKANLNVYISRELWDDLLSVLSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKL
  SEQKQKKHKGKGVGHEFQKVSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVDIDD
  AQILPRSTRVRHFSQSEETGNEVFGALNEEQPLPRSSSTSDILEPFTVERAKEAEQNATRGSTEGSVQS
  CNGLFWKESCSDIGGSSANVPDLMDEFIAERLRSGNASTMTRRGSSPGSLEIPKDLPDILNKQNQMRPI
  DDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFDNFGFGTDTGVTSSADVDSGSGHHQSAEE
  QEVASLTTLHIDSETSSLNQQAFSAEVATITGSESASPVHSPLGSRSQTPSPSTLNIDHMEQKDLQLDE
  KLHHSVLQTPDDLEISEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNSIMDPEIHAQVFDYL
  CELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICNTMKRRQ
  DVSPNRDFLTHFYNIMHCGLLHIDQDIVNTIIKHCSPQFFSLGLPGATMLIMDFIVAAGRVASSAFLNA
  PRVEAQVLLGSLVCFPNLYCELPSLHPNIPDVAVSQFTDVKELIIKTVLSSARDEPSGPARCVALCSLG
  IWICEELVHESHHPQIKEALNVICVSLKFTNKTVAHVACNMLHMLVHYVPRLQIYQPDSPLKIIQILIA
  TITHLLPSTEASSYEMDKRLVVSLLLCLLDWIMALPLKTLLQPFHATGAESDKTEKSVLNCIYKVLHGC
  VYGAQCFSNPRYFPMSLSDLASVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTQMQHGLISIA
  ARTVITHLVNHLGHYPMSGGPAMLTSQVCENHDNHYSESTELSPELFESPNIQFFVLNNTTLVSCIQIR
  SEENMPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPPPVSGLSEPTSFMLSLSHQEKPEEPPT
  SNECLEDITVKDGLSLQFKRFRETVPTWDTIRDEEDVLDELLQYLGVTSPECLQRTGISLNIPAPQPVC
  ISEKQENDVINAILKQHTEEKEFVEKHFNDLNMKAVEQDEPIPQKPQSAFYYCRLLLSILGMNSWDKRR
  SFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTN
  HCGFMGGLQKNKSTGLTTPYFATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRR
  GIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKVLPIMVRATAINASRALKSLIP
  LYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFSPAPYHHLPSDADH
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001283043
• ORF Size: 6147 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001283043.3
• RefSeq Size: 7921 bp
• RefSeq ORF: 6150 bp
• Locus ID: 253959
• Cytogenetics: 14q13.2
• MW: 231.6 kDa
• Gene Summary: This gene encodes a major subunit of the RAL-GTPase activating protein. A similar protein in mouse binds E12, a transcriptional regulator of immunoglobulin genes. The mouse protein also functions in skeletal muscle by binding to the regulatory 14-3-3 proteins upon stimulation with insulin or muscle contraction. A pseudogene of this gene has been identified on chromosome 9. [provided by RefSeq, Oct 2016]