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WNK2 (NM_001282394) Human Tagged ORF Clone
CAT#: RG240242
- TrueORF®
WNK2 (GFP-tagged) - Human WNK lysine deficient protein kinase 2 (WNK2), transcript variant 1
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Specifications
| Product Data | |
| Tag | TurboGFP |
| Symbol | WNK2 |
| Synonyms | NY-CO-43; P/OKcl.13; PRKWNK2; SDCCAG43 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG240242 representing NM_001282394.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGACGGCGATGGCGGCCGCCGAGACGTCCCCGGCACGCTGATGGAGCCCGGGCGCGGCGCGGGGCCC GCGGGCATGGCGGAGCCTCGGGCGAAGGCGGCGCGGCCGGGGCCCCAGCGCTTTCTGCGGCGCAGCGTG GTAGAGTCGGACCAGGAGGAGCCGCCGGGCTTGGAGGCAGCCGAGGCGCCGGGCCCGCAGCCCCCGCAG CCCCTGCAGCGCCGGGTGCTTCTGCTCTGCAAGACGCGCCGCCTCATCGCGGAGCGCGCCCGCGGACGC CCCGCCGCCCCCGCGCCCGCAGCGCTGGTAGCGCAGCCGGGAGCCCCCGGAGCCCCCGCGGACGCCGGC CCCGAGCCCGTGGGCACGCAGGAGCCCGGCCCGGACCCCATCGCAGCCGCTGTCGAAACCGCGCCTGCC CCCGACGGCGGCCCCAGGGAGGAGGCGGCGGCGACCGTGAGGAAGGAGGATGAGGGGGCGGCCGAGGCG AAGCCTGAGCCCGGGCGCACTCGCCGGGACGAGCCCGAAGAGGAGGAGGACGACGAGGACGACCTCAAG GCCGTGGCCACCTCTCTGGACGGCCGCTTCCTCAAGTTCGACATCGAGCTGGGCCGCGGTTCCTTCAAG ACGGTCTACAAGGGGCTGGACACGGAGACCTGGGTGGAGGTGGCCTGGTGTGAGCTGCAGGACCGGAAG CTCACCAAGCTGGAGCGGCAGCGGTTCAAGGAAGAGGCTGAGATGCTGAAAGGCCTGCAGCACCCCAAC ATCGTGCGCTTCTACGACTTCTGGGAGTCCAGCGCCAAGGGCAAGCGGTGCATTGTGCTGGTGACGGAG CTGATGACCTCAGGGACGCTGAAGACATACCTGAAGCGGTTCAAGGTGATGAAGCCCAAGGTTCTCCGC AGCTGGTGCCGGCAGATCCTGAAGGGCCTGCTGTTCCTGCACACAAGGACGCCACCCATCATCCACCGA GACCTGAAATGTGACAATATTTTCATCACCGGACCAACTGGGTCTGTGAAGATTGGCGACTTGGGCCTG GCCACTCTGAAAAGAGCGTCATTTGCCAAAAGTGTGATAGGTACTCCCGAGTTCATGGCGCCCGAGATG TACGAGGAGCACTACGATGAGTCCGTGGACGTCTATGCCTTTGGGATGTGCATGCTGGAGATGGCCACC TCGGAGTACCCCTACTCGGAGTGCCAGAATGCGGCCCAGATCTACCGCAAGGTCACCTGTGGTATCAAG CCGGCCAGCTTTGAGAAAGTGCACGATCCTGAAATCAAGGAGATTATTGGGGAGTGTATCTGCAAAAAC AAGGAGGAAAGGTACGAGATCAAAGACCTGCTGAGCCACGCCTTCTTCGCAGAGGACACAGGCGTGAGG GTGGAGCTCGCGGAGGAGGACCACGGCAGGAAGTCCACCATCGCCCTGAGGCTCTGGGTGGAAGACCCC AAGAAACTGAAGGGAAAGCCCAAGGACAATGGAGCCATAGAGTTCACCTTCGACCTGGAGAAGGAGACG CCGGATGAGGTGGCCCAAGAGATGATTGAGTCTGGATTCTTCCACGAGAGTGACGTCAAGATCGTGGCC AAGTCCATCCGTGACCGCGTGGCCTTGATCCAGTGGCGGCGGGAGAGGATCTGGCCCGCGCTGCAGCCC AAGGAGCAGCAGGATGTGGGCAGCCCGGACAAGGCCAGGGGTCCGCCGGTGCCCCTGCAGGTCCAGGTG ACCTACCATGCACAGGCTGGGCAGCCCGGGCCACCAGAGCCCGAGGAGCCGGAGGCCGACCAGCACCTC CTGCCACCTACGTTGCCGACCAGCGCCACCTCCCTGGCCTCGGACAGCACCTTCGACAGCGGCCAGGGC TCTACCGTGTACTCAGACTCGCAGAGCAGCCAGCAGAGCGTGATGCTTGGCTCCCTTGCCGACGCAGCG CCGTCCCCGGCCCAGTGTGTGTGCAGCCCCCCTGTGAGCGAGGGGCCCGTCCTGCCGCAGAGCCTGCCC TCGCTGGGGGCCTACCAGCAGCCCACGGCTGCACCTGGCTTGCCGGTGGGCTCTGTCCCGGCCCCCGCC TGCCCTCCGTCCCTCCAGCAGCACTTCCCGGATCCGGCCATGAGCTTCGCCCCCGTGCTGCCGCCGCCC AGCACCCCCATGCCCACGGGCCCAGGCCAGCCAGCACCCCCCGGCCAGCAGCCTCCTCCGCTGGCCCAG CCGACACCCCTGCCGCAGGTCCTGGCCCCACAGCCCGTGGTCCCCCTCCAGCCGGTTCCCCCCCACCTG CCACCGTACCTGGCTCCAGCCTCCCAGGTGGGGGCCCCCGCTCAGCTGAAGCCCCTCCAGATGCCACAG GCGCCCCTGCAGCCGCTTGCTCAAGTCCCTCCGCAGATGCCCCCGATTCCTGTTGTGCCCCCCATCACG CCCCTGGCGGGAATCGACGGCCTCCCTCCGGCCCTCCCAGACCTGCCGACCGCGACTGTGCCTCCCGTG CCACCACCTCAGTATTTCTCTCCAGCCGTGATCTTGCCGAGCCTCGCTGCCCCACTCCCCCCTGCGTCC CCAGCCTTGCCTCTGCAGGCTGTGAAGCTGCCCCACCCCCCTGGGGCGCCCCTGGCCATGCCCTGCCGG ACCATTGTGCCAAATGCACCGGCCACTATCCCCCTGCTGGCCGTAGCCCCACCGGGCGTGGCTGCCCTG TCCATTCATTCTGCCGTGGCCCAGCTCCCAGGCCAACCTGTGTACCCAGCGGCCTTCCCACAGATGGCG CCTACTGACGTCCCTCCTTCCCCCCATCACACGGTGCAGAATATGAGGGCCACCCCTCCACAGCCGGCA CTGCCTCCACAACCCACACTGCCCCCACAACCCGTGCTGCCCCCGCAACCCACGCTGCCCCCTCAACCT GTGTTGCCCCCGCAACCCACACGGCCCCCTCAACCTGTGCTGCCCCCGCAACCCATGCTGCCCCCACAA CCTGTGCTGCCCCCGCAGCCGGCACTGCCTGTGCGCCCTGAGCCCCTCCAGCCCCACCTTCCTGAACAA GCTGCTCCAGCTGCTACACCAGGGAGCCAGATTCTGCTTGGCCACCCAGCTCCCTATGCTGTGGACGTC GCCGCTCAGGTCCCCACCGTGCCTGTGCCACCGGCTGCGGTCCTCTCGCCGCCTCTGCCGGAAGTGCTG CTGCCTGCCGCCCCTGAGCTCCTGCCTCAGTTCCCCAGCTCCCTGGCCACGGTGTCTGCCTCTGTGCAG AGTGTGCCCACCCAGACTGCCACACTTCTGCCACCAGCAAACCCACCGCTGCCTGGCGGGCCCGGGATC GCCAGCCCTTGCCCAACTGTCCAGCTGACGGTGGAACCAGTCCAAGAGGAGCAGGCCTCACAGGACAAG CCGCCCGGCCTCCCGCAGAGCTGTGAGAGCTATGGAGGTTCTGATGTCACTTCTGGAAAAGAGCTGAGT GACAGCTGTGAAGGCGCCTTTGGAGGGGGCAGGCTGGAGGGCAGGGCAGCCCGAAAACACCACCGCAGG TCCACGCGTGCGCGCTCCCGGCAGGAGAGGGCCAGCCGGCCCCGGCTTACCATCTTGAACGTGTGCAAC ACTGGGGACAAGATGGTGGAGTGCCAGCTGGAGACGCACAACCACAAGATGGTGACCTTCAAGTTCGAC TTGGACGGGGACGCACCCGATGAAATTGCCACGTATATGGTGGAGCATGACTTTATCCTGCAGGCCGAG CGGGAAACGTTCATCGAGCAGATGAAGGATGTCATGGACAAGGCAGAGGACATGCTCAGCGAGGACACA GACGCCGACCGTGGCTCCGACCCAGGGACCAGCCCGCCACACCTCAGCACCTGCGGCCTGGGCACCGGG GAGGAGAGCCGACAATCCCAAGCCAACGCCCCCGTGTATCAGCAGAACGTCCTGCACACCGGGAAGAGG TGGTTCATCATCTGTCCGGTGGCTGAGCACCCCGCCCCCGAGGCCCCTGAATCTTCGCCCCCACTTCCT CTAAGCTCCCTGCCGCCAGAAGCCAGCCAAGGTCCATGCAGGGGGCTCACCCTGCCCTGTCTCCCATGG CGCAGAGCAGCCTGTGGGGCTGTGTTCCTATCCTTGTTTTCTGCTGAATCAGCTCAGTCAAAGCAGCCT CCAGATTCAGCGCCCTATAAAGACCAGCTGTCCTCGAAGGAACAACCCAGCTTTCTAGCCAGTCAGCAG CTCCTGAGCCAGGCGGGCCCCAGCAACCCTCCTGGGGCACCCCCAGCCCCTTTGGCCCCCTCCTCCCCT CCTGTGACTGCTCTGCCCCAAGATGGAGCAGCTCCAGCCACCAGCACCATGCCAGAGCCAGCGTCAGGA ACTGCCAGCCAGGCAGGGGGTCCAGGGACACCTCAGGGGCTGACCAGTGAGCTCGAGACGTCTCAGCCA CTAGCGGAGACTCACGAGGCCCCGCTTGCTGTGCAGCCCCTCGTGGTGGGCCTAGCACCTTGCACTCCA GCTCCAGAGGCTGCCTCAACCAGGGACGCCAGTGCCCCAAGGGAGCCCCTGCCACCTCCTGCACCTGAG CCCAGCCCCCACAGCGGGACCCCACAGCCCGCCTTGGGTCAACCTGCTCCCCTGCTTCCTGCCGCAGTG GGGGCCGTCAGCCTGGCCACCTCCCAGCTCCCAAGCCCACCCCTGGGGCCCACCGTCCCCCCACAGCCA CCCTCGGCCCTGGAGTCGGATGGGGAAGGGCCGCCCCCCAGGGTGGGCTTTGTGGACAGCACCATCAAG AGCCTGGACGAGAAGCTGCGGACTCTGCTCTACCAGGAGCACGTGCCCACCTCCTCAGCCTCAGCTGGG ACCCCTGTGGAGGTGGGCGACAGAGACTTCACCCTGGAGCCCCTGAGAGGGGACCAGCCCCGCTCAGAG GTCTGCGGGGGGGACCTGGCCCTGCCCCCAGTGCCTAAGGAGGCGGTCTCAGGGCGTGTCCAGCTGCCC CAGCCCTTGGTGGAGAAGTCAGAACTGGCCCCCACTCGAGGGGCCGTGATGGAGCAGGGCACGTCCTCG TCAATGACAGCAGAGTCGTCTCCCAGGAGTATGCTAGGCTATGACAGAGATGGAAGGCAGGTGGCCTCA GACTCCCATGTGGTCCCCAGCGTCCCCCAGGATGTACCTGCTTTTGTGAGACCTGCACGTGTGGAGCCC ACAGACAGGGATGGTGGAGAAGCTGGAGAAAGCTCGGCAGAGCCCCCGCCGAGTGACATGGGCACAGTG GGGGGCCAGGCTAGCCACCCCCAGACACTCGGCGCTCGAGCTTTGGGGTCCCCTCGGAAACGTCCAGAG CAGCAGGATGTCAGCTCACCAGCCAAGACTGTGGGCCGTTTCTCGGTGGTCAGCACTCAGGACGAGTGG ACCCTGGCCTCCCCCCACAGCCTGAGATACTCTGCCCCACCCGACGTCTACCTGGACGAGGCCCCCTCC AGCCCCGACGTGAAGCTGGCAGTGCGGCGGGCGCAGACGGCCTCCTCCATCGAGGTCGGCGTGGGCGAG CCCGTGTCCAGCGACTCTGGGGACGAGGGCCCTCGGGCGAGACCCCCGGTGCAGAAGCAGGCGTCCCTG CCCGTGAGTGGCAGCGTGGCTGGCGACTTCGTGAAGAAGGCCACCGCCTTCCTGCAGAGGCCTTCTCGG GCCGGCTCGCTGGGCCCCGAGACACCCAGCAGGGTGGGCATGAAGGTCCCCACGATCAGCGTGACCTCC TTCCATTCCCAGTCGTCCTACATCAGCAGCGACAATGATTCGGAGCTCGAGGATGCTGACATAAAGAAG GAGCTGCAGAGTCTGCGGGAGAAGCACCTGAAGGAGATCTCGGAGCTGCAGAGCCAGCAGAAGCAGGAG ATCGAAGCTCTGTACCGCCGCCTGGGCAAGCCACTGCCCCCCAACGTGGGCTTCTTCCACACGGCACCC CCCACTGGCCGCCGGAGAAAAACCAGCAAGAGCAAGCTGAAGGCAGGCAAGCTGCTAAATCCCCTGGTG CGGCAGCTCAAGGTCGTGGCCTCCAGCACAGGTCACTTGGCTGACTCCAGCAGAGGCCCTCCCGCTAAG GACCCTGCCCAAGCCAGTGTGGGGCTCACTGCAGACAGCACGGGCCTGAGCGGGAAGGCAGTGCAGACC CAGCAGCCCTGCTCCGTCCGGGCCTCCCTGTCTTCGGACATCTGCTCCGGCTTAGCCAGTGATGGAGGC GGAGCGCGTGGCCAAGGCTGGACGGTTTACCACCCAACGTCTGAGAGAGTGACCTATAAGTCTAGTAGC AAACCTCGTGCTCGATTCCTCAGTGGACCCGTATCTGTGTCCATCTGGTCTGCCCTGAAGCGTCTCTGC CTAGGCAAAGAACACAGCAGTAGGTCCTCCACCAGCAGCCTGGCCCCAGGCCCTGAGCCAGGCCCCCAG CCCGCCCTGCACGTCCAGGCGCAGGTGAACAACAGCAACAACAAGAAGGGTACCTTCACGGACGACCTG CACAAGCTGGTGGACGAGTGGACGAGCAAGACGGTGGGGGCCGCGCAGCTGAAGCCCACGCTCAACCAG CTGAAGCAGACCCAGAAGCTGCAAGACATGGAGGCCCAGGCAGGCTGGGCTGCCCCTGGCGAGGCGCGG GCTATGACCGCACCTCGAGCAGGAGTGGGGATGCCACGTCTGCCCCCAGCGCCCGGCCCTCTGTCCACC ACGGTCATTCCCGGAGCCGCCCCGACCCTGTCCGTGCCCACACCAGATGGCGCCCTCGGAACCGCCCGG AGAAACCAGGTGTGGTTTGGCCTCCGAGTCCCCCCCACCGCCTGCTGTGGGCACAGCACTCAGCCGCGA GGGGGACAGCGGGTGGGCAGCAAGACTGCTTCCTTTGCGGCTTCAGACCCTGTTCGCTCC AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC >Peptide sequence encoded by RG240242
Blue=ORF Red=Cloning site Green=Tag(s) MDGDGGRRDVPGTLMEPGRGAGPAGMAEPRAKAARPGPQRFLRRSVVESDQEEPPGLEAAEAPGPQPPQ PLQRRVLLLCKTRRLIAERARGRPAAPAPAALVAQPGAPGAPADAGPEPVGTQEPGPDPIAAAVETAPA PDGGPREEAAATVRKEDEGAAEAKPEPGRTRRDEPEEEEDDEDDLKAVATSLDGRFLKFDIELGRGSFK TVYKGLDTETWVEVAWCELQDRKLTKLERQRFKEEAEMLKGLQHPNIVRFYDFWESSAKGKRCIVLVTE LMTSGTLKTYLKRFKVMKPKVLRSWCRQILKGLLFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGL ATLKRASFAKSVIGTPEFMAPEMYEEHYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTCGIK PASFEKVHDPEIKEIIGECICKNKEERYEIKDLLSHAFFAEDTGVRVELAEEDHGRKSTIALRLWVEDP KKLKGKPKDNGAIEFTFDLEKETPDEVAQEMIESGFFHESDVKIVAKSIRDRVALIQWRRERIWPALQP KEQQDVGSPDKARGPPVPLQVQVTYHAQAGQPGPPEPEEPEADQHLLPPTLPTSATSLASDSTFDSGQG STVYSDSQSSQQSVMLGSLADAAPSPAQCVCSPPVSEGPVLPQSLPSLGAYQQPTAAPGLPVGSVPAPA CPPSLQQHFPDPAMSFAPVLPPPSTPMPTGPGQPAPPGQQPPPLAQPTPLPQVLAPQPVVPLQPVPPHL PPYLAPASQVGAPAQLKPLQMPQAPLQPLAQVPPQMPPIPVVPPITPLAGIDGLPPALPDLPTATVPPV PPPQYFSPAVILPSLAAPLPPASPALPLQAVKLPHPPGAPLAMPCRTIVPNAPATIPLLAVAPPGVAAL SIHSAVAQLPGQPVYPAAFPQMAPTDVPPSPHHTVQNMRATPPQPALPPQPTLPPQPVLPPQPTLPPQP VLPPQPTRPPQPVLPPQPMLPPQPVLPPQPALPVRPEPLQPHLPEQAAPAATPGSQILLGHPAPYAVDV AAQVPTVPVPPAAVLSPPLPEVLLPAAPELLPQFPSSLATVSASVQSVPTQTATLLPPANPPLPGGPGI ASPCPTVQLTVEPVQEEQASQDKPPGLPQSCESYGGSDVTSGKELSDSCEGAFGGGRLEGRAARKHHRR STRARSRQERASRPRLTILNVCNTGDKMVECQLETHNHKMVTFKFDLDGDAPDEIATYMVEHDFILQAE RETFIEQMKDVMDKAEDMLSEDTDADRGSDPGTSPPHLSTCGLGTGEESRQSQANAPVYQQNVLHTGKR WFIICPVAEHPAPEAPESSPPLPLSSLPPEASQGPCRGLTLPCLPWRRAACGAVFLSLFSAESAQSKQP PDSAPYKDQLSSKEQPSFLASQQLLSQAGPSNPPGAPPAPLAPSSPPVTALPQDGAAPATSTMPEPASG TASQAGGPGTPQGLTSELETSQPLAETHEAPLAVQPLVVGLAPCTPAPEAASTRDASAPREPLPPPAPE PSPHSGTPQPALGQPAPLLPAAVGAVSLATSQLPSPPLGPTVPPQPPSALESDGEGPPPRVGFVDSTIK SLDEKLRTLLYQEHVPTSSASAGTPVEVGDRDFTLEPLRGDQPRSEVCGGDLALPPVPKEAVSGRVQLP QPLVEKSELAPTRGAVMEQGTSSSMTAESSPRSMLGYDRDGRQVASDSHVVPSVPQDVPAFVRPARVEP TDRDGGEAGESSAEPPPSDMGTVGGQASHPQTLGARALGSPRKRPEQQDVSSPAKTVGRFSVVSTQDEW TLASPHSLRYSAPPDVYLDEAPSSPDVKLAVRRAQTASSIEVGVGEPVSSDSGDEGPRARPPVQKQASL PVSGSVAGDFVKKATAFLQRPSRAGSLGPETPSRVGMKVPTISVTSFHSQSSYISSDNDSELEDADIKK ELQSLREKHLKEISELQSQQKQEIEALYRRLGKPLPPNVGFFHTAPPTGRRRKTSKSKLKAGKLLNPLV RQLKVVASSTGHLADSSRGPPAKDPAQASVGLTADSTGLSGKAVQTQQPCSVRASLSSDICSGLASDGG GARGQGWTVYHPTSERVTYKSSSKPRARFLSGPVSVSIWSALKRLCLGKEHSSRSSTSSLAPGPEPGPQ PALHVQAQVNNSNNKKGTFTDDLHKLVDEWTSKTVGAAQLKPTLNQLKQTQKLQDMEAQAGWAAPGEAR AMTAPRAGVGMPRLPPAPGPLSTTVIPGAAPTLSVPTPDGALGTARRNQVWFGLRVPPTACCGHSTQPR GGQRVGSKTASFAASDPVRS SGPTRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLL SHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGF PEDSVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGP MFAFRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001282394 |
| ORF Size | 6891 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
| Reference Data | |
| RefSeq | NM_001282394.1, NP_001269323.1 |
| RefSeq Size | 8240 bp |
| RefSeq ORF | 6894 bp |
| Locus ID | 65268 |
| Cytogenetics | 9q22.31 |
| Protein Families | Druggable Genome, Protein Kinase |
| MW | 243.1 kDa |
| Gene Summary | The protein encoded by this gene is a cytoplasmic serine-threonine kinase that belongs to the protein kinase superfamily. The protein plays an important role in the regulation of electrolyte homeostasis, cell signaling survival, and proliferation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013] |
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