Dnmt3a (NM_001003958) Rat Untagged Clone

CAT#: RN200037

Dnmt3a (untagged ORF) - Rat DNA (cytosine-5-)-methyltransferase 3 alpha (Dnmt3a), transcript variant 1, (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Dnmt3a
• Synonyms: MGC109195
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN200037 representing NM_001003958
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCTCCAGCGGCCCCGGGGACACCAGCATCTCCTCTCTGGAGCGGGAGGATGATCGAAAGGAAGGAG
  AGGAACAGGAGGAGAACCGTGGTAAGGAAGAGCGTCAGGAGCCCAGCGCCACGGCCCGGAAAGTGGGGAG
  GCCTGGCCGGAAGCGCAAGCACCCACCGGTGGAAAGCAGTGACACCCCCAAGGACCCAGCGGTGACCACC
  AAGTCTCAGCCCACAGCCCAGGACTCTGGGCCCTCAGATCTGCTACCCAATGGAGACTTGGAGAAGCGGA
  GTGAACCCCAACCTGAGGAGGGGAGCCCAGCTGCAGGGCAGAAGGGTGGGGCCCCAGCTGAAGGAGAGGG
  AACTGAGACCCCACCAGAAGCCTCCAGAGCAGTGGAGAATGGCTGCTGCGTAACCAAGGAAGGCCGTGGA
  GCCTCTGCGGGAGAGGGCAAAGAACAGAAGCAGACCAACATCGAATCCATGAAAATGGAGGGCTCCCGGG
  GCCGACTGCGTGGTGGCTTGGGGTGGGAGTCCAGCCTCCGTCAGCGGCCCATGCCAAGACTCACCTTCCA
  GGCAGGGGACCCCTACTACATCAGCAAACGGAAGCGGGATGAGTGGCTGGCACGTTGGAAAAGGGAGGCT
  GAGAAGAAAGCCAAGGTGATTGCAGTAATGAATGCTGTGGAGGAAAGCCAGGCCTCTGGGGAGTCCCAGA
  AGGTGGAGGAGGCCAGCCCTCCTGCAGTGCAGCAGCCCACGGACCCTGCATCTCCCACTGTGGCCACCAC
  CCCTGAGCCAGTAGGGGCTGATGCTGGGGACAAGAATGCTACCAAGGCAGCTGATGACGAGCCCGAGTAT
  GAGGATGGCCGGGGCTTTGGCATTGGAGAGCTGGTGTGGGGGAAACTCCGGGGCTTCTCCTGGTGGCCAG
  GCCGAATTGTGTCTTGGTGGATGACAGGCCGGAGCCGAGCAGCCGAAGGCACTCGCTGGGTCATGTGGTT
  CGGAGATGGCAAATTCTCAGTGGTGTGTGTCGAGAAGCTCATGCCCCTGAGCTCCTTCTGCAGTGCGTTC
  CACCAGGCCACCTACAACAAGCAGCCCATGTACCGCAAAGCCATCTACGAAGTCCTCCAGGTGGCCAGCA
  GCCGTGCAGGGAAGCTGTTCCCAGCATGTCATGACAGCGATGAAAGTGACACTGGCAAGGCTGTGGAGGT
  GCAGAACAAGCAGATGATTGAGTGGGCCCTTGGCGGCTTCCAGCCCTCTGGTCCCAAAGGCCTGGAGCCA
  CCAGAAGAGGAGAAGAATCCTTACAAGGAAGTTTACACCGACATGTGGGTGGAGCCTGAGGCAGCTGCTT
  ATGCCCCACCCCCACCAGCCAAGAAACCCAGAAAGAGCACAACAGAGAAACCCAAGGTCAAGGAGATCAT
  TGATGAACGCACAAGAGAACGGCTCGTGTATGAGGTGCGCCAGAAGTGCCGAAACATCGAGGACATTTGT
  ATCTCATGTGGGAGCCTCAATGTTACCCTGGAGCACCCACTCTTCATTGGTGGAATGTGCCAGAACTGTA
  AGAACTGCTTCTTGGAGTGTGCTTACCAATACGATGACGATGGGTACCAGTCCTACTGTACCATCTGCTG
  TGGGGGGCGCGAAGTGCTCATGTGTGGGAACAACAACTGCTGCAGGTGCTTTTGTGTGGAGTGTGTGGAT
  CTCTTGGTGGGGCCAGGGGCTGCCCAAGCAGCCATTAAGGAAGACCCCTGGAACTGCTACATGTGTGGGC
  ACAAGGGCACGTATGGGCTGCTGCGGAGACGGGAGGACTGGCCTTCTCGACTCCAGATGTTCTTCGCCAA
  TAACCACGACCAGGAATTTGACCCCCCGAAGGTTTACCCACCTGTGCCAGCTGAGAAGAGGAAGCCCATC
  CGGGTGCTATCTCTCTTTGATGGGATTGCTACAGGGCTCCTGGTGCTGAAGGACCTGGGCATCCAAGTGG
  ACCGCTACATCGCCTCCGAGGTGTGCGAGGACTCCATCACAGTGGGCATGGTGCGGCACCAGGGAAAGAT
  CATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGGAGTGGGGCCCATTCGATCTGGTG
  ATTGGGGGCAGTCCCTGCAATGACCTCTCCATCGTCAACCCTGCCCGCAAGGGACTTTACGAGGGCACGG
  GCCGCCTCTTCTTTGAGTTCTACCGCCTCCTGCATGACGCGCGGCCCAAGGAGGGAGATGATCGCCCCTT
  CTTCTGGCTTTTTGAGAATGTGGTGGCCATGGGCGTTAGTGACAAGAGGGACATCTCGAGATTTCTTGAG
  TCTAACCCCGTGATGATCGACGCCAAAGAAGTGTCTGCTGCACACAGGGCCCGTTATTTCTGGGGTAACC
  TTCCTGGCATGAACAGGCCATTGGCATCCACTGTGAATGATAAGCTGGAGTTGCAAGAGTGTCTGGAACA
  CGGCAGAATAGCCAAGTTCAGCAAAGTGAGGACCATTACCACCAGGTCAAACTCCATAAAGCAGGGCAAA
  GACCAGCATTTCCCTGTCTTCATGAATGAGAAGGAAGACATCCTCTGGTGCACTGAAATGGAAAGGGTGT
  TTGGCTTCCCTGTCCACTACACAGACGTCTCCAACATGAGCCGCTTGGCGAGGCAGAGACTGCTGGGCCG
  ATCGTGGAGCGTGCCAGTCATCCGCCACCTCTTCGCTCCGCTGAAGGAATATTTTGCTTGTGTGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001003958
• Insert Size: 2727 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001003958.1, NP_001003958.1
• RefSeq Size: 4173 bp
• RefSeq ORF: 2727 bp
• Locus ID: 444984
• Cytogenetics: 6q14
• Gene Summary: member of a family of DNA methyltransferases [RGD, Feb 2006]