Bicd2 (NM_001033674) Rat Untagged Clone

CAT#: RN200101

Bicd2 (untagged ORF) - Rat bicaudal D homolog 2 (Drosophila) (Bicd2), transcript variant 1, (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Bicd2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN200101 representing NM_001033674
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCGCGCCGTCGGAGGAGGAGGAGTATGCGCGTCTGGTGATGGAGGCGCAGCCGGAGTGGCTGCGCG
  CCGAGGTGAAGCGACTGTCCCACGAGCTGGCCGAAACCACGCGTGAGAAGATCCAGGCGGCCGAGTATGG
  ACTGGCGGTGCTTGAGGAGAAGCACCAGCTCAAGCTGCAGTTCGAGGAGCTCGAGGTGGACTATGAGGCC
  ATCCGCAGCGAGATGGAGCAGCTCAAAGAGGCATTTGGCCAGGCACACACAAACCACAAGAAGGTGGCTG
  CTGATGGAGAGAGCCGGGAGGAGAGCCTGATCCAGGAGTCCGCCTCTAAGGAGCAGTACTATGTGCGGAA
  GGTGCTGGAGCTGCAGACAGAGCTGAAACAGCTGCGCAATGTCCTTATCAACACTCAGTCTGAGAATGAG
  CGCCTCACGTCTGTGGCCCAGGAGCTGAAAGAGATCAACCAGAATGTGGAGATTCAGCGTGGTCGCCTGC
  GAGATGACATCAAGGAGTACAAGTTCCGGGAGGCCCGTCTACTTCAGGACTACTCTGAGCTGGAGGAGGA
  GAACATTAGCCTGCAGAAACAAGTGTCTGTGCTCAGGCAGAACCAGGTGGAATTTGAGGGCCTCAAGCAT
  GAAATCAAGCGTCTGGAGGAGGAGACAGAGTACCTTAACAGCCAGCTGGAGGATGCCATCCGGCTTAAGG
  AGATCTCTGAACGGCAGTTGGAGGAAGCGCTGGAGACGTTGAAGACAGAGCGAGAGCAGAAGAACAACCT
  ACGTAAGGAGTTGTCGCACTACATGAGCATCAACGATTCCTTCTATACCAGCCACCTGCAGGTATCCTTA
  GATGGCCTCAAGTTCAGTGATGATGCTGTCACTGCTGAGCCCAACAACGACGCCGAAGCCCTGGTCAATG
  GCTTTGAGCACAGCGGCTTGGTCAAAGCGTCGTTGGACAACAAGACATCCACACCCAGAAAGGATGGCCT
  GGCTCCGCCCTCCCCCAGCCTTGTTTCCGACCTGCTCAGTGAGCTCCACATATCTGAGATCCAGAAGCTG
  AAGCAGCAGCTGGTGCAGATGGAGCGGGAGAAGGTGGGTCTGCTGGCGACATTACAGGACACACAGAAGC
  AGCTAGAGCAGGCTCGGGGAACCCTCTCTGAGCAACATGAGAAGGTGAGTCGTCTCACAGAGAACCTTAG
  TGCCCTCCGGCGCCTGCAGGCTGGCAAGGAGCGACAGACTTCCCTGGATAACGAAAAGGACCGAGATAGC
  CATGAAGACGGTGACTACTATGAGGTTGACATCAATGGGCCTGAGATCCTGTCCTGCAAGTACCATGTAG
  CTGTGGCCGAGGCTGGCGAGCTCCGGGAGCAGCTCAAGGCCCTGCACAGCACACATGAAGCTCGGGAAGC
  CCAGCTTGCAGAAGAAAAGGGCCGCTATGAGGCTGAGGTCCAGGCTCTCACTGAGAAGGTGTCACTGCTG
  GAGAAGGCTAGCCACCAGGACCAAGAGCTGCTGGCCCAGCTGGAAAAGGAGCTGAGGAAGGTGAGTGACG
  TGGCTGGTGAGACCCAGGGCAGCCTGAATGTGGCTCAGGATGAGTTGGTGACCTTCAGCGAGGAGCTGGC
  CAATCTCTACCACCATGTGTGCATGTGCAACAACGAGACACCCAACCGTGTCATGCTGGACTATTACCGT
  GAGGGGCAGGGCAAGGCTGGCCGCACCAGCCCAGAAGGCCGCGGGCGCCGCTCGCCTGTCCTTTTGCCCA
  AGGGGCTGTTGTCGACAGAGATCGGCCGAGCAGATGGTGGGACTGGGGACAACAGCCCTTCACCCAGCTC
  TTCACTGCCATCACCATTGAGTGACCCCCGCCGGGAACCTATGAACATCTACAACCTGATCGCCATCATC
  CGAGACCAGATCAAGCACCTGCAGGCAGCCGTAGATCGTACAACCGAGCTGTCCCGGCAGCGGATTGCCT
  CACAGGAGCTGGGCCCTGCTGTGGATAAGGACAAGGAAGCACTCATGGAGGAGATCCTCAAGCTGAAGTC
  ACTGCTGAGTACCAAGAGGGAACAGATCACCACACTTCGTACTGTGCTCAAGGCTAACAAGCAGACGGCA
  GAGGTGGCCCTCGCCAACCTGAAGAGCAAGTATGAGAATGAGAAGGCCATGGTGACCGAGACCATGATGA
  AGCTGCGGAACGAGCTGAAGGCCCTCAAGGAGGACGCAGCCACCTTCTCCTCCCTGCGTGCCATGTTCGC
  CACCAGGTGTGATGAGTACATCACGCAGCTGGATGAGATGCAGCGGCAGCTGGCGGCTGCCGAGGATGAG
  AAGAAGACTCTTAATTCCCTGCTGCGCATGGCCATCCAGCAGAAGCTGGCACTCACCCAGCGGCTGGAGC
  TGCTGGAGCTGGACCATGAGCAGACCCGCAGGGGCCGTAACAAGGCCACCTCCAAGGCCAAGCCAGCCAC
  ACCGAGCGTAAGTCACACCTGCGCCTGTGCCAGCGAGAGGGCGGAGGGCACCGGGCTGGCCAACCAGGTG
  TTCTGCAGCGAGAAGCACAGCATTTACTGTGATTAG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_001033674
• Insert Size: 2556 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001033674.2, NP_001028846.1
• RefSeq Size: 6324 bp
• RefSeq ORF: 2556 bp
• Locus ID: 306809
• Cytogenetics: 17p14
• Gene Summary: This gene encodes a dynein-dynactin adaptor protein. In mouse, the protein primarily localizes to the Golgi complex as well as to microtubule plus ends through dynactin. Overexpression studies suggest that the N-terminus mediates the dynein-dynactin interaction, while the C-terminus is responsible for Golgi targeting. Knockout of this gene in mouse results in a defect in cerebellar granule cell migration. In human, mutations in this gene are associated with spinal muscular dystrophy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2015]
  Transcript Variant: This variant (1) represents the longer variant and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.