Thra (NM_001017960) Rat Untagged Clone

CAT#: RN200271

Thra (untagged ORF) - Rat thyroid hormone receptor alpha (Thra), transcript variant TRalpha1, (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Thra
• Synonyms: c-erbA-1; ERBA1; Thra1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN200271 representing NM_001017960
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAACAGAAGCCAAGCAAGGTGGAGTGTGGGTCAGACCCAGAGGAGAACAGTGCCAGGTCACCAGATG
  GAAAGCGAAAAAGGAAGAACGGCCAATGTCCCCTGAAAAGCAGCATGTCAGGGTATATCCCTAGTTACCT
  GGACAAAGACGAGCAGTGTGTCGTGTGTGGGGACAAGGCCACCGGTTATCACTACCGCTGTATCACTTGT
  GAGGGCTGCAAGGGCTTCTTTCGCCGTACAATCCAGAAGAACCTCCATCCCACCTATTCCTGCAAATATG
  ACAGCTGCTGCGTCATCGACAAGATCACCCGGAATCAGTGCCAGTTGTGCCGCTTCAAGAAGTGCATCGC
  TGTGGGCATGGCCATGGACCTGGTTCTAGACGATTCAAAGCGGGTGGCCAAACGCAAGCTGATTGAGCAG
  AACCGGGAGAGGAGGCGAAAGGAGGAGATGATCCGCTCGCTGCAGCAACGACCAGAGCCCACTCCTGAAG
  AGTGGGATCTGATCCACGTTGCTACAGAGGCCCATCGCAGCACCAATGCCCAGGGCAGCCATTGGAAACA
  GAGGCGAAAATTCCTGCCGGATGACATTGGCCAGTCACCTATTGTCTCCATGCCGGACGGAGACAAGGTA
  GACCTAGAGGCCTTCAGCGAGTTTACCAAGATCATCACCCCGGCCATCACCCGCGTGGTGGACTTTGCCA
  AAAAACTGCCCATGTTCTCCGAGCTGCCTTGTGAAGACCAGATCATCCTCCTGAAGGGCTGCTGCATGGA
  GATCATGTCCCTGCGGGCAGCTGTCCGCTATGACCCTGAGAGCGACACCCTGACCCTGAGTGGGGAGATG
  GCGGTTAAGCGGGAGCAGCTCAAGAATGGTGGCTTGGGTGTAGTCTCCGACGCCATCTTTGAACTGGGCA
  AGTCACTCTCTGCCTTTAACCTGGATGATACGGAAGTGGCTCTGCTGCAGGCTGTGCTGTTAATGTCAAC
  AGACCGCTCTGGCCTGCTGTGTGTGGACAAGATCGAGAAGAGTCAGGAGGCCTACCTGCTGGCGTTTGAG
  CACTACGTCAACCACCGCAAACACAACATTCCGCACTTCTGGCCCAAGCTGCTGATGAAGGTGACTGACC
  TCCGCATGATCGGGGCCTGCCACGCCAGCCGCTTCCTCCACATGAAAGTCGAGTGCCCCACCGAACTCTT
  CCCCCCACTCTTCCTGGAGGTCTTTGAGGATCAGGAAGTCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001017960
• Insert Size: 1233 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001017960.1, NP_001017960.1
• RefSeq Size: 2243 bp
• RefSeq ORF: 1233 bp
• Locus ID: 81812
• Cytogenetics: 10q31
• Gene Summary: binds the promoter of the Na+/H+ exchanger NHE1 and mediates thyroid hormone induced transcriptional activation [RGD, Feb 2006]
  Transcript Variant: This variant (TRalpha1) differs in the 3' UTR and coding region compared to TRalpha2, resulting in a shorter isoform (TRalpha1) with a distinct C-terminus. The TRalpha1 isoform, also known as c-erbAalpha1, functions as a thyroid hormone-dependent transcription factor.