Mcam (NM_001034009) Rat Untagged Clone

CAT#: RN200301

Mcam (untagged ORF) - Rat melanoma cell adhesion molecule (Mcam), transcript variant 2, (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Mcam
• Synonyms: CD146; Muc18
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN200301 representing NM_001034009
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGCTGCCCAGGCTGGTCTGCGCCTTCTTATTCGCTGCCTGCTGCTGCTGTCGCAGCGCCACGGGTG
  TGCCAGGAGAGGAAAAGCAGCCCACACCCACGCCGGACCCGGTGGAGGTAGAAGTGGGCAACACGGCCCT
  TCTGAAGTGTGGCCCCGCACATCCCTCAGGCAACTTCAGCCAAGTGGAATGGTTTTTGATTCACAAGGAG
  AGGCAGATACCCATTTTCCGTGTGCACCAGGGCAAGGGCCAGAGTGAACCTGGGGAATATGAACACCGCC
  TCAGCCTCCACGGCCCAGGAGCTACTCTGGCCCTGAGTCAAGTCACTCCCCATGATGATCGGATGTTCCT
  GTGTAAGAGCAAGCAGCCACGGCCCCAGGATCACTATGTTCAGCTGCAAGTCTACAAGGCTCCAGAGGAG
  CCAACTATCCAAGCCAACGTGCTGGGCATCCACGTGGACATACAAGAGCTTAAGGAGGTTGCTACCTGTG
  TGGGGAGGAATGGCTACCCCATTCCTCAAGTCATCTGGTACAAGAATGGCCGGCCCTTGCAAGAGGAGGA
  GAACCGCGTTCATATCCAGTCGTCACAGACCGTGGAGTCCAGTGGCTTGTACACCTTGAAGAGTGTTCTA
  AGTGCACGGCTAGTCAAGGAGGACAAAGATGCCCAGTTTTACTGTGAACTCAGCTACCGGCTGCCCAGTG
  GGAACCGCATGAAGGAATCTAAGGAGGTCACTGTCCCTGTCTTATACCCTGCAGAAAAAGTGTGGGTGGA
  GGTAGAACCCGTGGGACTGCTGAAGGAAGGGGACCATGTGAAGATCAGGTGTCTGACTGACGGCAACCCT
  CAACCCCACTTCACTATCAACAAGAAGAACCCCAGCACTGAGGAGATGGAAGAGGAAAGCACCGATGAAA
  ACGGTCTCCTGTCCTTGGAGCCCGCCCAGAAGCACCACAGTGGGGTCTACCAATGTCAGAGCCTAGACTT
  GGAAACTACAGTTATGCTGTCGAGTGACCCCCTGGAGCTGCTGGTGAACTATGTGTCTGATGTTCAAGTG
  GATCCAACTGCCCCTGAAGTCCAGGAAGGTGATAGCCTCACACTGACCTGTAAGGCAGAGAGTAACCAGG
  ACCTTGAGTTTGAGTGGCTGAGAGACAAGACAGGCCAGCTTCTGGGAAAGGGGCCCATCCTTCAGCTAAA
  CAACGTGAAAAGGGAAGCAGGGGGAAGATATCTCTGTGTGGCATCTGTCCCCAGCGTTCCTGGCTTGAAT
  CGCACACGGCGGGTCAGCGTGGGCATTTTTGGGTCCCCGTGGATGGCAGCAAAGGAGAGGAAGGTGTGGG
  CGCAAGAGAATGCAATGCTGAATCTGTCTTGTGAGGCTTCAGGCCATCCTCAGCCCACCATCTCCTGGAA
  TATCAATGGCTCGGCAACTGAATGGAACCCAGATCCTCAGACAGTAGTGAGCACCTTGAATGTCCTTGTG
  ACCCCAGAGCTTCTGGAGACCGGTGCAGAATGTACAGCCTCCAACTCCCTGGGCTCAAACACCACGGTCA
  TCATTCTGAAGCTCGTCACTTTAACCACCCTCACACCTGACTCCAGCCAAACCACTGGCCTCAGCACCCC
  CACAGTCAGTCCTCACAGCAGAGCCAACAGCACCTCCACAGAGAAAAAGCTGCCACAGCAGGAGAGCAAA
  GGTGTGGTCATCGTGGCTGTGATAGTATGTACGCTGGTGCTTGCTGTACTGGGCGCTACCCTCTATTACT
  TCTACAAGAAGGGCAAACTGCCTTGTGGACGCTCAGGAAAACAGGAGATGGAGAGAAATACATCGATCTG
  A
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001034009
• Insert Size: 1821 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001034009.1, NP_001029181.1
• RefSeq Size: 2815 bp
• RefSeq ORF: 1821 bp
• Locus ID: 78967
• Cytogenetics: 8q22
• Gene Summary: may act as a cell adhesion molecule; may play a role in tumor growth and invasion [RGD, Feb 2006]