Asic2 (NM_001034014) Rat Untagged Clone

CAT#: RN200428

Asic2 (untagged ORF) - Rat amiloride-sensitive cation channel 1, neuronal (Accn1), transcript variant 2, (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Asic2
• Synonyms: Accn1; BNaC1; BNC1k; MDEG; MDEG1; MDEG2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN200428 representing NM_001034014
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACCTCAAGGAGAGCCCCAGTGAGGGCAGCCTGCAACCTTCCAGTATCCAGATCTTCGCCAATACCT
  CCACTCTCCATGGCATCCGCCACATCTTCGTGTATGGGCCGCTGACCATCCGGCGTGTGCTTTGGGCAGT
  GGCCTTCGTCGGATCCCTGGGCCTGCTGCTGGTGGAGAGCTCGGAGAGGGTGTCCTACTATTTCTCTTAT
  CAGCATGTTACCAAGGTGGATGAAGTGGTGGCCCAGAGCCTGGTCTTCCCAGCTGTGACCCTCTGCAACC
  TCAATGGCTTCCGGTTCTCCAGGCTTACCACCAACGACTTGTACCACGCTGGGGAGTTGCTGGCCCTGCT
  GGATGTCAACCTACAGATTCCCGACCCGCATCTGGCAGACCCCACGGTGCTGGAGGCCCTCCGACAGAAG
  GCCAACTTCAAACACTACAAACCGAAGCAGTTCAGCATGCTGGAGTTCCTGCACCGGGTAGGCCATGACC
  TGAAGGATATGATGCTCTACTGCAAGTTCAAGGGGCAGGAGTGTGGGCATCAAGACTTCACCACAGTGTT
  TACAAAATACGGGAAGTGTTACATGTTTAACTCAGGCGAGGATGGCAAGCCGCTGCTCACCACGGTCAAG
  GGGGGGACGGGCAACGGGCTGGAGATCATGCTGGACATTCAGCAAGATGAGTACCTGCCCATCTGGGGAG
  AGACAGAGGAAACAACGTTTGAAGCAGGAGTGAAGGTTCAGATCCACAGTCAGTCTGAGCCGCCTTTCAT
  CCAAGAGCTGGGCTTTGGGGTGGCTCCGGGGTTCCAGACCTTCGTGGCCACACAAGAGCAGAGGCTCACA
  TATCTGCCCCCACCATGGGGGGAGTGCCGGTCCTCAGAGATGGGACTCGACTTCTTTCCTGTTTACAGCA
  TCACAGCCTGTCGGATTGACTGTGAGACCCGCTACATCGTGGAGAACTGTAACTGCCGCATGGTCCACAT
  GCCAGGGGACGCCCCTTTCTGCACCCCTGAGCAGCACAAGGAGTGTGCAGAGCCTGCCCTCGGTCTACTG
  GCAGAAAAGGACAGCAATTACTGTCTCTGCAGGACACCCTGCAACCTGACACGCTACAACAAAGAGCTCT
  CCATGGTGAAGATCCCCAGCAAGACGTCAGCCAAGTACTTAGAGAAGAAATTTAACAAATCGGAAAAATA
  TATCTCAGAGAACATTCTTGTTCTGGACATATTTTTTGAGGCGCTCAATTACGAAACAATTGAACAGAAG
  AAGGCGTATGAAGTTGCTGCCTTACTTGGTGACATCGGTGGTCAGATGGGACTGTTCATTGGTGCTAGTC
  TCCTCACAATACTAGAGCTCTTTGATTATATTTATGAGCTGATCAAAGAGAAGCTATTAGACCTGCTTGG
  CAAAGAAGAAGAGGAAGGGAGCCACGATGAGAACATGAGCACCTGTGACACAATGCCAAACCACTCTGAA
  ACCATCAGCCACACTGTGAACGTGCCCCTGCAGACAGCTTTGGGCACCCTGGAGGAGATTGCCTGCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001034014
• Insert Size: 1539 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001034014.1, NP_001029186.1
• RefSeq Size: 2565 bp
• RefSeq ORF: 1539 bp
• Locus ID: 25364
• Cytogenetics: 10q26
• Gene Summary: member of the degenerin/epithelial Na(+) channel superfamily; permeable for Na(+), K(+) and Li(+); mutations may lead to a form of hereditary neurodegeneration; may be important for pain sensation [RGD, Feb 2006]
  Transcript Variant: This variant (MDEG1) uses an alternate 5' terminal exon compared to variant MDEG2. The resulting protein (isoform MDGEG1) is shorter and has a distinct N-terminus compared to isoform MDEG2.